Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Super-resolved multispectral lensless microscopy via angle-tilted, wavelength-multiplexed ptychographic modulation

Abstract

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at five wavelengths simultaneously. A prism is added at the illumination path for spectral dispersion. Thus, lightwaves at different wavelengths hit the specimen at slightly different incident angles, breaking the ambiguities in mixed-state ptychographic reconstruction. At the detection path, we place a thin diffuser between the specimen and the monochromatic image sensor for encoding the spectral information into 2D intensity measurements. By scanning the sample to different $x\!-\!y$ positions, we acquire a sequence of monochromatic images for reconstructing the five complex object profiles at the five wavelengths. An up-sampling procedure is integrated into the recovery process to bypass the resolution limit imposed by the imager pixel size. We demonstrate a half-pitch resolution of 0.55 µm using an image sensor with 1.85 µm pixel size. We also demonstrate quantitative and high-quality multispectral reconstructions of stained tissue sections for digital pathology applications.

© 2020 Optical Society of America

Full Article  |  PDF Article
More Like This
High-throughput lensless whole slide imaging via continuous height-varying modulation of a tilted sensor

Shaowei Jiang, Chengfei Guo, Patrick Hu, Derek Hu, Pengming Song, Tianbo Wang, Zichao Bian, Zibang Zhang, and Guoan Zheng
Opt. Lett. 46(20) 5212-5215 (2021)

Field-portable quantitative lensless microscopy based on translated speckle illumination and sub-sampled ptychographic phase retrieval

He Zhang, Zichao Bian, Shaowei Jiang, Jian Liu, Pengming Song, and Guoan Zheng
Opt. Lett. 44(8) 1976-1979 (2019)

Super-resolution microscopy via ptychographic structured modulation of a diffuser

Pengming Song, Shaowei Jiang, He Zhang, Zichao Bian, Chengfei Guo, Kazunori Hoshino, and Guoan Zheng
Opt. Lett. 44(15) 3645-3648 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (4)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved