Abstract

Hilbert–Huang single-shot spatially multiplexed interferometric microscopy (H2S2MIM) is presented as the implementation of a robust, fast, and accurate single-shot phase estimation algorithm with an extremely simple, low-cost, and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. Altogether, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available nonholographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20×/0.46  NA objective in a regular Olympus BX-60 upright microscope for static, as well as dynamic, samples showing perfect agreement with the results retrieved from a temporal phase-shifting algorithm.

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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References

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2013 (3)

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[Crossref]

F. Merola, L. Miccio, M. Paturzo, A. Finizio, S. Grilli, and P. Ferraro, Opt. Lett. 36, 3079 (2011).
[Crossref]

2010 (3)

2009 (1)

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T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, Opt. Lett. 30, 1165 (2005).
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G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 10, 060503 (2005).
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2002 (1)

2001 (1)

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[Crossref]

Anand, A.

Badizadegan, K.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 10, 060503 (2005).
[Crossref]

Best, C.

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 10, 060503 (2005).
[Crossref]

Bhaduri, B.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Bon, P.

Burton, D. R.

Chen, Z.

Chhaniwal, V.

Choi, W.

Choi, Y.

Dan, D.

Dasari, R. R.

Dorn, A.

Edwards, C.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Ellenbogen, T.

Falaggis, K.

Feld, M. S.

Ferraro, P.

Ferreira, C.

J. A. Picazo-Bueno, Z. Zalevsky, J. García, C. Ferreira, and V. Micó, J. Bio. Opt. 21, 106007 (2016).
[Crossref]

V. Mico, C. Ferreira, Z. Zalevsky, and J. García, Opt. Express 22, 14929 (2014).
[Crossref]

Finizio, A.

Fu, D.

Gao, P.

Garcia, J.

García, J.

Gdeisat, M. A.

Girshovitz, P.

Goddard, L. L.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Grilli, S.

Guo, R.

Han, J.

Herráez, M. A.

Huang, N. E.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Ikeda, T.

T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, Opt. Lett. 30, 1165 (2005).
[Crossref]

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 10, 060503 (2005).
[Crossref]

Javidi, B.

Karepov, S.

Kemper, B.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[Crossref]

B. Kemper and G. von Bally, Appl. Opt. 47, A52 (2008).
[Crossref]

Kim, B. M.

Kim, H. J.

Kim, M. K.

M. K. Kim, SPIE Rev. 1, 018005 (2010).
[Crossref]

M. K. Kim, Digital Holographic Microscopy: Principles, Techniques, and Applications, 1st ed. (Springer, 2011).

Kozacki, T.

Lalor, M. J.

Larkin, K. G.

Lee, K. J.

Lei, M.

Leitgeb, R. A.

Liu, H. H.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Long, S. R.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Martinez-Carranza, J.

Maucort, G.

Merola, F.

Miccio, L.

Mico, V.

Micó, V.

J. Á. Picazo-Bueno, Z. Zalevsky, J. García, and V. Micó, Opt. Lett. 42, 927 (2017).
[Crossref]

J. A. Picazo-Bueno, Z. Zalevsky, J. García, C. Ferreira, and V. Micó, J. Bio. Opt. 21, 106007 (2016).
[Crossref]

Min, J.

Monneret, S.

Nguyen, T. H.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Oh, S.

Patorski, K.

Paturzo, M.

Pham, H.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Picazo-Bueno, J. A.

J. A. Picazo-Bueno, Z. Zalevsky, J. García, C. Ferreira, and V. Micó, J. Bio. Opt. 21, 106007 (2016).
[Crossref]

Picazo-Bueno, J. Á.

Popescu, G.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 10, 060503 (2005).
[Crossref]

T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, Opt. Lett. 30, 1165 (2005).
[Crossref]

G. Popescu, Quantitative Phase Imaging of Cells and Tissues (McGraw-Hill Biophotonics, 2011).

Roitshtain, D.

Rommel, C. E.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[Crossref]

Schnekenburger, J.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[Crossref]

Shaked, N. T.

Sheng, Z.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Shih, W. H.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Singh, A. S. G.

Sluzewski, L.

Tang, C.

Tearney, G. J.

Trusiak, M.

Tung, C. C.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Turko, N. A.

Vollmer, A.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[Crossref]

von Bally, G.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[Crossref]

B. Kemper and G. von Bally, Appl. Opt. 47, A52 (2008).
[Crossref]

Wattellier, B.

Wielgus, M.

M. Trusiak, M. Wielgus, and K. Patorski, Opt. Laser Eng. 52, 230 (2014).
[Crossref]

M. Trusiak, K. Patorski, and M. Wielgus, Opt. Express 20, 23463 (2012).
[Crossref]

Wu, M. C.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Yamauchi, T.

Yan, S.

Yang, C.

Yang, T. D.

Yang, Y.

Yao, B.

Yaqoob, Z.

Ye, T.

Yen, N. C.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Yu, X.

Zalevsky, Z.

Zeng, Q.

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

Zhou, R.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Zhu, X.

Adv. Opt. Photonics (1)

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, Adv. Opt. Photonics 6, 57 (2014).
[Crossref]

Appl. Opt. (4)

J. Bio. Opt. (1)

J. A. Picazo-Bueno, Z. Zalevsky, J. García, C. Ferreira, and V. Micó, J. Bio. Opt. 21, 106007 (2016).
[Crossref]

J. Biomed. Opt. (2)

G. Popescu, T. Ikeda, C. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. 10, 060503 (2005).
[Crossref]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, J. Biomed. Opt. 16, 026014 (2011).
[Crossref]

J. Opt. Soc. Am. A (1)

Opt. Express (8)

Opt. Laser Eng. (1)

M. Trusiak, M. Wielgus, and K. Patorski, Opt. Laser Eng. 52, 230 (2014).
[Crossref]

Opt. Lett. (10)

Proc. R. Soc. London A (1)

N. E. Huang, Z. Sheng, S. R. Long, M. C. Wu, W. H. Shih, Q. Zeng, N. C. Yen, C. C. Tung, and H. H. Liu, Proc. R. Soc. London A 454, 903 (1998).
[Crossref]

SPIE Rev. (1)

M. K. Kim, SPIE Rev. 1, 018005 (2010).
[Crossref]

Other (3)

M. K. Kim, Digital Holographic Microscopy: Principles, Techniques, and Applications, 1st ed. (Springer, 2011).

N. T. Shaked, Z. Zalevsky, and L. L. Satterwhite, eds., Biomedical Optical Phase Microscopy and Nanoscopy (Academic, 2012).

G. Popescu, Quantitative Phase Imaging of Cells and Tissues (McGraw-Hill Biophotonics, 2011).

Supplementary Material (5)

NameDescription
» Visualization 1       Digital slightly off-axis recording (static micro bead) from which temporal P-S and S2H2MIM algorithms are applied to retrieve QPI
» Visualization 2       Digital slightly off-axis recording (prostate cancer cells type I) from which temporal P-S and S2H2MIM algorithms are applied to retrieve QPI
» Visualization 3       Digital slightly off-axis recording (prostate cancer cells type II) from which temporal P-S and S2H2MIM algorithms are applied to retrieve QPI
» Visualization 4       Digital slightly off-axis recording from which S2H2MIM algorithm is applied to retrieve QPI
» Visualization 5       Retrieved phase from S2H2MIM technique for the dynamic sample

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Figures (4)

Fig. 1.
Fig. 1. Optical layout and ray tracing for H2S2MIM. Reconstructions come from the lower half of the CCD area where the O ( 0 th ) + R ( 1 st ) beams overlap.
Fig. 2.
Fig. 2. Calibration results for a 90 μm sphere: (a) single slightly off-axis interferogram (first frame of the full P-S sequence Visualization 1, 3.25 MB); (b) the FT of (a); (c) the complex pupil retrieved by a P-S algorithm; (d) the intensity image; (e)–(f) and (g)–(h) phase visualization modes from P-S and H2S2MIM, respectively (lateral scales represent optical phase in rads); and (i) the comparison plot of the 3D phase profiles at (f) and (h). The scale bars at the lower right corners are 50 μm, approximately.
Fig. 3.
Fig. 3. Experiments with prostate cancer cells: (a) and (d) full available FOVs; (b) and (e) 2D unwrapped phase distribution incoming from the P-S algorithm; and (c) and (f) the distribution from H2S2MIM. Reconstructions from the white solid-line ROIs in (a) and (d). The lateral scale represents the optical phase in radians, and the scale bars are 50 μm, approximately.
Fig. 4.
Fig. 4. Dynamic experimental validation of H2S2MIM using 45 μm flowing beads: (a) the first frame of the whole recorded P-S sequence (Visualization 4, 1.36 MB) and (b) the same (Visualization 5, 0.25 MB) concerning 2D unwrapped phase distribution retrieved from H2S2MIM. The lateral scale represents the optical phase in rads. The scale bars at lower right corners are 25 μm, approximately.

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