Abstract

A novel hyperspectral single pixel system was used to compare different compressive basis patterns for intensity imaging, lifetime imaging, and FRET quantification. Six popular basis patterns were compared experimentally in a phantom containing two fluorescent dyes. The basis patterns that performed best for lifetime quantification were used to measure FRET occurrence in well-plate samples with varying acceptor–donor ratios. The ABS-WP approach using Haar patterns and the compressive sensing approach with Hadamard Ranked patterns displayed the best overall performances at a 50% compression ratio.

© 2018 Optical Society of America

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Supplementary Material (6)

NameDescription
» Visualization 1       Visualization 1.
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» Visualization 4       Visualization 4.
» Visualization 5       Visualization 5.
» Visualization 6       Visualization 6.

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