Abstract

Genetically-encoded calcium indicators (GECIs) have revolutionized neuroimaging by enabling mapping of the activity of entire neuronal populations in vivo. Visualization of these powerful activity sensors has to date been limited to depth-restricted microscopic studies due to intense light scattering in the brain. We demonstrate, for the first time, in vivo real-time volumetric optoacoustic monitoring of calcium transients in adult transgenic zebrafish expressing the GCaMP5G calcium indicator. Fast changes in optoacoustic traces associated with GCaMP5G activity were detectable in the presence of other strongly absorbing endogenous chromophores, such as hemoglobin. The new functional optoacoustic neuroimaging method can visualize neural activity at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques.

© 2017 Optical Society of America

Hybrid system for in vivo epifluorescence and 4D optoacoustic imaging

Zhenyue Chen, Xosé Luis Deán-Ben, Sven Gottschalk, and Daniel Razansky
Opt. Lett. 42(22) 4577-4580 (2017)

In vivo three-dimensional characterization of the adult zebrafish brain using a 1325 nm spectral-domain optical coherence tomography system with the 27 frame/s video rate

Jian Zhang, Wei Ge, and Zhen Yuan
Biomed. Opt. Express 6(10) 3932-3940 (2015)

In vivo three dimensional dual wavelength photoacoustic tomography imaging of the far red fluorescent protein E2-Crimson expressed in adult zebrafish

Mengyang Liu, Nicole Schmitner, Michelle G. Sandrian, Behrooz Zabihian, Boris Hermann, Willi Salvenmoser, Dirk Meyer, and Wolfgang Drexler
Biomed. Opt. Express 4(10) 1846-1855 (2013)

Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy

Sean Quirin, Nikita Vladimirov, Chao-Tsung Yang, Darcy S. Peterka, Rafael Yuste, and Misha B. Ahrens
Opt. Lett. 41(5) 855-858 (2016)

Functional in vivo imaging using fluorescence lifetime light-sheet microscopy

Claire A. Mitchell, Simon P. Poland, James Seyforth, Jakub Nedbal, Thomas Gelot, Tahiyat Huq, Gerhard Holst, Robert D. Knight, and Simon M. Ameer-Beg
Opt. Lett. 42(7) 1269-1272 (2017)