Fluorescence lifetime imaging (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. There has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed endoscopy systems. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of endoscopy systems that are suitable for in vivo tissue imaging. We present a compact wide-field time-gated FLIM flexible endoscope capable of continuous lifetime imaging of up to three fluorescence emission bands simultaneously. This endoscope design will facilitate the evaluation of FLIM for in vivo applications.
© 2013 Optical Society of AmericaFull Article | PDF Article
Jan Siegel, Daniel S. Elson, Stephen E. D. Webb, K. C. Benny Lee, Alexis Vlandas, Giovanni L. Gambaruto, Sandrine Lévêque-Fort, M. John Lever, Paul J. Tadrous, Gordon W. H. Stamp, Andrew L. Wallace, Ann Sandison, Tim F. Watson, Fernando Alvarez, and Paul M. W. French
Appl. Opt. 42(16) 2995-3004 (2003)
Sun Young Park, Tom Collier, Jesse Aaron, Mia K. Markey, Rebecca Richards-Kortum, Konstantin Sokolov, Nick Mackinnon, Calum MacAulay, Lezlee Coghlan, Andrea Milbourne, and Michele Follen
Opt. Express 13(3) 749-762 (2005)
Yinghua Sun, Jennifer Phipps, Daniel S. Elson, Heather Stoy, Steven Tinling, Jeremy Meier, Brian Poirier, Frank S. Chuang, D. Gregory Farwell, and Laura Marcu
Opt. Lett. 34(13) 2081-2083 (2009)