This Letter presents a technique for subdiffraction limit imaging termed Bessel beam microscopy (BBM). By placing a lens in series with an axicon in the optical path of a microscope, the diffraction-limited resolution of the base microscope is improved by one third. This improvement is demonstrated experimentally by resolving individual subdiffraction limit fluorescent beads in a close-pack arrangement. The behavior of the BBM system is explored using angular diffraction simulations, demonstrating the possibility of resolving features spaced as little as 110 nm apart when viewed with a objective. Unique among super-resolution techniques, BBM acquires subdiffraction limit information in a single image with broadband unstructured illumination using only static geometric optics placed between the microscope and camera.
© 2013 Optical Society of AmericaFull Article | PDF Article
OSA Recommended Articles
Opt. Lett. 38(14) 2550-2553 (2013)
Steve Wereley, Yuxing Zhang, Jian-Wei Khor, and Craig Snoeyink
Appl. Opt. 55(35) 10025-10029 (2016)
Kenneth W. Allen, Navid Farahi, Yangcheng Li, Nicholaos I. Limberopoulos, Dennis E. Walker, Augustine M. Urbas, and Vasily N. Astratov
Opt. Express 23(19) 24484-24496 (2015)