We demonstrate efficient generation of wide-field fluorescence signals in two-photon microscopy exploiting diffractive optical elements and short pulses by using a dispersion-compensated beam delivery optics module. Computer-generated holograms are codified onto a phase-only spatial light modulator, which allows for arbitrary single-shot patterning of the sample. Spatiotemporal shaping of the pulse is mandatory to overcome spatial chirp and pulse-front tilt effects that spread both in space and time the irradiance patterns, thus limiting not only the spatial resolution but also the signal-to-noise ratio in two-photon microscopy. By using a multipass amplifier delivering 30 fs, 0.8 mJ pulses at 1 kHz repetition rate, we experimentally demonstrated arbitrary single-shot fluorescence irradiance patterns in Rhodamine B.
© 2013 Optical Society of AmericaFull Article | PDF Article
Haifeng Wang, Terry B. Huff, and Ji-Xin Cheng
Opt. Lett. 31(10) 1417-1419 (2006)
Michael E. Durst, Guanghao Zhu, and Chris Xu
Opt. Express 14(25) 12243-12254 (2006)
Adam M. Larson and Alvin T. Yeh
Opt. Express 16(19) 14723-14730 (2008)