Abstract

Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but it sacrifices chemical specificity in samples with overlapping bands compared to broadband (multiplex) excitation. We develop a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multicolor SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.

© 2013 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Generation of synchronized picosecond pulses by a 1.06-µm gain-switched laser diode for stimulated Raman scattering microscopy

Kyoya Tokunaga, Yi-Cheng Fang, Hiroyuki Yokoyama, and Yasuyuki Ozeki
Opt. Express 24(9) 9617-9628 (2016)

Intracavity wavelength modulation of an optical parametric oscillator for coherent Raman microscopy

Brian G. Saar, Gary R. Holtom, Christian W. Freudiger, Chrisita Ackermann, Winfield Hill, and X. Sunney Xie
Opt. Express 17(15) 12532-12539 (2009)

Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging

Zi Wang, Wei Zheng, and Zhiwei Huang
Opt. Lett. 41(17) 3960-3963 (2016)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription