Membrane permeabilization is imperative for gene and drug delivery systems, along with other cell manipulation methods, since the average eukaryotic cell membrane is not permeable to polar and large nonpolar molecules. Antibody conjugated optically absorbing gold nanospheres are targeted to the cell membrane of T47D breast cancer cell line and irradiated with 5 ns pulse, 20 Hz, 532 nm light to increase membrane permeability. Up to 90% permeabilization with less than 6% death is reported at radiant exposures up to 10 times lower than those of other comparable studies.
© 2012 Optical Society of AmericaFull Article | PDF Article
D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T. A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, and K. Dholakia
Opt. Express 14(16) 7125-7133 (2006)
Srivalleesha Mallidi, Timothy Larson, Jesse Aaron, Konstantin Sokolov, and Stanislav Emelianov
Opt. Express 15(11) 6583-6588 (2007)
J. Baumgart, W. Bintig, A. Ngezahayo, S. Willenbrock, H. Murua Escobar, W. Ertmer, H. Lubatschowski, and A. Heisterkamp
Opt. Express 16(5) 3021-3031 (2008)