Abstract

Recently it has been demonstrated that digital holography is a powerful means allowing imaging of both amplitude and phase objects in turbid flowing media. However, in quasi-static turbid microfluidics, multiple scattering contributions through the colloids superimpose coherently to the recording device, resulting in speckle noise and hindering a clear vision of the objects. In this Letter we exploit the Brownian motion of the colloidal particles to get multiple uncorrelated holograms, and we combine them to reduce the speckle contrast. In this way we get a multi-look gain without losing image resolution.

© 2012 Optical Society of America

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References

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2012 (2)

X. Yang, H. Chia-Lung, P. Ye, and D. Psaltis, Opt. Express 20, 2500 (2012).
[CrossRef]

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

2011 (1)

N. Pandey and B. Hennelly, Proc. SPIE 8074, 807404 (2011).
[CrossRef]

2010 (4)

W. Bishara, H. Zhu, and A. Ozcan, Opt. Express 18, 27499 (2010).
[CrossRef]

R. Srivastava, J. R. P. Gupta, and H. Partasarthy, Opt. Lasers Eng. 48, 626 (2010).
[CrossRef]

X. Cai, Optik 121, 394 (2010).
[CrossRef]

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

2008 (1)

F. Monroy, O. Rincon, Y. M. Torres, and J. G. Sucerquia, Opt. Commun. 281, 3454 (2008).
[CrossRef]

2006 (1)

2005 (1)

J. G. Sucerquia, J. A. H. Ramirez, and D. V. Prieto, Optik 116, 44 (2005).
[CrossRef]

1976 (1)

Balduzzi, D.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Baumbach, T.

Bishara, W.

Cai, X.

X. Cai, Optik 121, 394 (2010).
[CrossRef]

Chia-Lung, H.

Ferraro, P.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Finizio, A.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Galli, A.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Goodmann, J. W.

Gupta, J. R. P.

R. Srivastava, J. R. P. Gupta, and H. Partasarthy, Opt. Lasers Eng. 48, 626 (2010).
[CrossRef]

Hennelly, B.

N. Pandey and B. Hennelly, Proc. SPIE 8074, 807404 (2011).
[CrossRef]

Juptner, W.

Kebbel, V.

Kolenovic, E.

Li, R.

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

Liu, S.

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

Memmolo, P.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Monroy, F.

F. Monroy, O. Rincon, Y. M. Torres, and J. G. Sucerquia, Opt. Commun. 281, 3454 (2008).
[CrossRef]

Ozcan, A.

Pan, F.

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

Pandey, N.

N. Pandey and B. Hennelly, Proc. SPIE 8074, 807404 (2011).
[CrossRef]

Partasarthy, H.

R. Srivastava, J. R. P. Gupta, and H. Partasarthy, Opt. Lasers Eng. 48, 626 (2010).
[CrossRef]

Paturzo, M.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Prieto, D. V.

J. G. Sucerquia, J. A. H. Ramirez, and D. V. Prieto, Optik 116, 44 (2005).
[CrossRef]

Psaltis, D.

Puglisi, R.

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Ramirez, J. A. H.

J. G. Sucerquia, J. A. H. Ramirez, and D. V. Prieto, Optik 116, 44 (2005).
[CrossRef]

Rincon, O.

F. Monroy, O. Rincon, Y. M. Torres, and J. G. Sucerquia, Opt. Commun. 281, 3454 (2008).
[CrossRef]

Rong, L.

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

Srivastava, R.

R. Srivastava, J. R. P. Gupta, and H. Partasarthy, Opt. Lasers Eng. 48, 626 (2010).
[CrossRef]

Sucerquia, J. G.

F. Monroy, O. Rincon, Y. M. Torres, and J. G. Sucerquia, Opt. Commun. 281, 3454 (2008).
[CrossRef]

J. G. Sucerquia, J. A. H. Ramirez, and D. V. Prieto, Optik 116, 44 (2005).
[CrossRef]

Torres, Y. M.

F. Monroy, O. Rincon, Y. M. Torres, and J. G. Sucerquia, Opt. Commun. 281, 3454 (2008).
[CrossRef]

Xiao, W.

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

Yang, X.

Ye, P.

Zhu, H.

Appl. Opt. (1)

Chinese Opt. Lett. (1)

L. Rong, W. Xiao, F. Pan, S. Liu, and R. Li, Chinese Opt. Lett. 8, 653 (2010).
[CrossRef]

J. Opt. Soc. Am. (1)

Lab On Chip (1)

M. Paturzo, A. Finizio, P. Memmolo, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, Lab On Chip 12, 3073 (2012).
[CrossRef]

Opt. Commun. (1)

F. Monroy, O. Rincon, Y. M. Torres, and J. G. Sucerquia, Opt. Commun. 281, 3454 (2008).
[CrossRef]

Opt. Express (2)

Opt. Lasers Eng. (1)

R. Srivastava, J. R. P. Gupta, and H. Partasarthy, Opt. Lasers Eng. 48, 626 (2010).
[CrossRef]

Optik (2)

X. Cai, Optik 121, 394 (2010).
[CrossRef]

J. G. Sucerquia, J. A. H. Ramirez, and D. V. Prieto, Optik 116, 44 (2005).
[CrossRef]

Proc. SPIE (1)

N. Pandey and B. Hennelly, Proc. SPIE 8074, 807404 (2011).
[CrossRef]

Supplementary Material (4)

» Media 1: AVI (4097 KB)     
» Media 2: AVI (3511 KB)     
» Media 3: AVI (1858 KB)     
» Media 4: AVI (6914 KB)     

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Figures (6)

Fig. 1.
Fig. 1.

Amplitude images of spermatozoa in turbid medium. (a) N=1, v=0μm/s; (b) N=250, v=0μm/s; (c) N=25 (decimated), v=0μm/s (Media 1); and (d) N=1, v=330μm/s.

Fig. 2.
Fig. 2.

Normalized speckle contrast versus N.

Fig. 3.
Fig. 3.

ML improvement versus v.

Fig. 4.
Fig. 4.

Amplitude images. (a) v=0μm/s, N=1; (b) v=0μm/s, N=25; (c) v=30μm/s, N=1; and (d) v=30μm/s, N=25 (Media 2).

Fig. 5.
Fig. 5.

Image amplitudes in the box under test.

Fig. 6.
Fig. 6.

Phase images. (a) v=0μm/s, N=1; (b) v=0μm/s, N=50 (Media 3); (c) v=30μm/s, N=1; and (d) v=30μm/s, N=25 (Media 4).

Equations (2)

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I=|k=1K|Ok|exp(jψk)|2,
C=σμ,

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