Abstract

We developed a new full off-axis red-green-blue (RGB) digital holographic microscope with an LED illumination. A decisive advantage of the use of LED illumination is a large image quality improvement due to its partially coherent nature. The off-axis configuration enables the fast recording of the holographic data in each spectral channel. The digital holographic refocusing and the optical phase map computation are successfully demonstrated. The multiwavelength operation provides a significant improvement of the collected information for colored samples.

© 2012 Optical Society of America

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  1. F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
    [CrossRef]
  2. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
    [CrossRef]
  3. N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
    [CrossRef]
  4. T. Zhang and I. Yamaguchi, Opt. Lett. 23, 1221 (1998).
    [CrossRef]
  5. B. Javidi, I. Moon, S. Yeom, and E. Carapezza, Opt. Express 13, 4492 (2005).
    [CrossRef]
  6. M. Paturzo and P. Ferraro, Opt. Lett. 34, 3650 (2009).
    [CrossRef]
  7. F. Dubois, L. Joannes, and J.-C. Legros, Appl. Opt. 38, 7085 (1999).
    [CrossRef]
  8. H. Ding and G. Popescu, Opt. Express 18, 1569 (2010).
    [CrossRef]
  9. P. Kolman and R. Chmelík, Opt. Express 18, 21990 (2010).
    [CrossRef]
  10. Z. Monemhaghdoust, F. Montfort, Y. Emery, C. Depeursinge, and C. Moser, Opt. Express 19, 24005 (2011).
    [CrossRef]
  11. J. Min, B. Yao, P. Gao, R. Guo, B. Ma, J. Zheng, M. Lei, S. Yan, D. Dan, T. Duan, Y. Yang, and T. Ye, Appl. Opt. 51, 191 (2012).
    [CrossRef]
  12. A. Calabuig, V. Micó, J. Garcia, Z. Zalevsky, and C. Ferreira, Opt. Lett. 36, 885 (2011).
    [CrossRef]
  13. J.-I. Kato, I. Yamaguchi, and T. Matsumura, Opt. Lett. 27, 1403 (2002).
    [CrossRef]
  14. P. Picart, P. Tankam, D. Mounier, Z. Peng, and J. Li, Opt. Express 17, 9145 (2009).
    [CrossRef]
  15. S. De Nicola, A. Finizio, G. Pierattini, D. Alfieri, S. Grilli, L. Sansone, and P. Ferraro, Opt. Lett. 30, 2706 (2005).
    [CrossRef]
  16. P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. De Nicola, A. Finizio, and B. Javidi, J. Display Tech. 4, 97 (2008).
    [CrossRef]
  17. P. Ferraro, L. Miccio, S. Grilli, M. Paturzo, S. De Nicola, A. Finizio, R. Osellame, and P. Laporta, Opt. Express 15, 14591 (2007).
    [CrossRef]

2012

2011

2010

2009

2008

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. De Nicola, A. Finizio, and B. Javidi, J. Display Tech. 4, 97 (2008).
[CrossRef]

2007

2006

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

2005

2002

1999

1998

Alfieri, D.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. De Nicola, A. Finizio, and B. Javidi, J. Display Tech. 4, 97 (2008).
[CrossRef]

S. De Nicola, A. Finizio, G. Pierattini, D. Alfieri, S. Grilli, L. Sansone, and P. Ferraro, Opt. Lett. 30, 2706 (2005).
[CrossRef]

Allano, D.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Biomed, J.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Calabuig, A.

Carapezza, E.

Chmelík, R.

Coëtmellec, S.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Colomb, T.

Cuche, E.

Dan, D.

De Nicola, S.

Debeir, O.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Decaestecker, C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Depeursinge, C.

Ding, H.

Duan, T.

Dubois, F.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

F. Dubois, L. Joannes, and J.-C. Legros, Appl. Opt. 38, 7085 (1999).
[CrossRef]

Emery, Y.

Ferraro, P.

Ferreira, C.

Finizio, A.

Gao, P.

Garcia, J.

Godard, G.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Grilli, S.

Guo, R.

Javidi, B.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. De Nicola, A. Finizio, and B. Javidi, J. Display Tech. 4, 97 (2008).
[CrossRef]

B. Javidi, I. Moon, S. Yeom, and E. Carapezza, Opt. Express 13, 4492 (2005).
[CrossRef]

Joannes, L.

Kato, J.-I.

Kiss, R.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Kolman, P.

Laporta, P.

Lebrun, D.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Legros, J.-C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

F. Dubois, L. Joannes, and J.-C. Legros, Appl. Opt. 38, 7085 (1999).
[CrossRef]

Lei, M.

Li, J.

Ma, B.

Magistretti, P. J.

Marquet, P.

Matsumura, T.

Miccio, L.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. De Nicola, A. Finizio, and B. Javidi, J. Display Tech. 4, 97 (2008).
[CrossRef]

P. Ferraro, L. Miccio, S. Grilli, M. Paturzo, S. De Nicola, A. Finizio, R. Osellame, and P. Laporta, Opt. Express 15, 14591 (2007).
[CrossRef]

Micó, V.

Min, J.

Monemhaghdoust, Z.

Monnom, O.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Montfort, F.

Moon, I.

Moser, C.

Mounier, D.

Osellame, R.

Paranthoën, P.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Paturzo, M.

Peng, Z.

Picart, P.

Pierattini, G.

Popescu, G.

Rappaz, B.

Salah, N.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Sansone, L.

Tankam, P.

Van Ham, Ph.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Yamaguchi, I.

Yan, S.

Yang, Y.

Yao, B.

Ye, T.

Yeom, S.

Yourassowsky, C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Zalevsky, Z.

Zhang, T.

Zheng, J.

Appl. Opt.

J. Display Tech.

P. Ferraro, S. Grilli, L. Miccio, D. Alfieri, S. De Nicola, A. Finizio, and B. Javidi, J. Display Tech. 4, 97 (2008).
[CrossRef]

Meas. Sci. Technol.

N. Salah, G. Godard, D. Lebrun, P. Paranthoën, D. Allano, and S. Coëtmellec, Meas. Sci. Technol. 19, 074001 (2008).
[CrossRef]

Opt.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, Ph. Van Ham, R. Kiss, C. Decaestecker, and J. Biomed, Opt. 11–5, 054032 (2006).
[CrossRef]

Opt. Express

Opt. Lett.

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Figures (4)

Fig. 1.
Fig. 1.

The optical setup. SO: The RGB LED module, LG: liquid light guide, L1–L7: achromat lenses, P: aperture, M1–M4: mirrors, BS1 and BS2: broadband plate beamsplitters, ML1, ML2: microscope lenses, SA: sample, C: optical path compensator, G: grating, S: optical stop, RA: rotation assembly, CCD: camera. ND: neutral density filter to adjust the beam intensity ratio.

Fig. 2.
Fig. 2.

Parts of holograms of a red stained wood tissue slice, recorded unfocused: (a) red; (b) green; (c) blue. The scale bar=100μm. They are 350×350 pixel zones cropped in 1344×1024 holograms.

Fig. 3.
Fig. 3.

(a) Out of focus recorded RGB intensity image; (b) refocused RGB intensity image by digital holography.

Fig. 4.
Fig. 4.

Phase images of the refocused holograms of the images of Fig. 2: (a) red; (b) green; (c) blue.

Equations (2)

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go(x,y,ν)=Aexp{jk2(f6+f7)}exp{jKf6f7x}×gi(f6f7x,f6f7x)s(ν),
go(x,y,t)=Aδ(t2(f6+f7)c)exp{jKf6f7x}×gi(f6f7x,f6f7x).

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