Abstract

This Letter presents the τ interferometer, a portable and inexpensive device for obtaining spatial interferograms of microscopic biological samples without the strict stability and the highly coherent illumination that are usually required for interferometric microscopy setups. The device is built using off-the-shelf optical elements and can easily operate with low-coherence illumination, while being positioned in the output of a conventional inverted microscope. The interferograms are processed into the quantitative amplitude and phase profiles of the sample. Based on the phase profile, the optical-path-delay profile is obtained with temporal stability of 0.18 nm and spatial stability of 0.42 nm. Further experimental demonstration of using the τ interferometer for imaging the quantitative thickness profile of a live red blood cell is provided.

© 2012 Optical Society of America

Full Article  |  PDF Article
Related Articles
Diffraction phase and fluorescence microscopy

YongKeun Park, Gabriel Popescu, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld
Opt. Express 14(18) 8263-8268 (2006)

Single-shot Full-field reflection phase microscopy

Zahid Yaqoob, Toyohiko Yamauchi, Wonshik Choi, Dan Fu, Ramachandra R. Dasari, and Michael S. Feld
Opt. Express 19(8) 7587-7595 (2011)

Automated imaging, identification, and counting of similar cells from digital hologram reconstructions

Mona Mihailescu, Mihaela Scarlat, Alexandru Gheorghiu, Julia Costescu, Mihai Kusko, Irina Alexandra Paun, and Eugen Scarlat
Appl. Opt. 50(20) 3589-3597 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription