Abstract

Although ultrasound arrays have been exploited in photoacoustic imaging to improve imaging speed, ultrasound-array-based optical-resolution photoacoustic microscopy (OR-PAM) has never been achieved previously to our knowledge. Here we present our development of multifocal OR-PAM using a microlens array for optical illumination and an ultrasound array for photoacoustic detection. Our system is capable of imaging hemoglobin concentration and oxygenation in individual microvessels in vivo at high speed. Compared with a single focus, multiple foci reduce the scanning load and increase the imaging speed significantly. The current multifocal system can acquire 1000×500×200 voxels at 10μm lateral resolution within 4min.

© 2011 Optical Society of America

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2010 (3)

2009 (3)

2008 (3)

2005 (1)

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

2004 (1)

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

2003 (1)

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef] [PubMed]

1975 (1)

M. D. Stern, Nature 254, 56 (1975).
[CrossRef] [PubMed]

An, L.

Audinat, E.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef] [PubMed]

Bitton, R.

L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
[CrossRef] [PubMed]

R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, Opt. Express 16, 7915 (2008).
[CrossRef] [PubMed]

Chaigneau, E.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef] [PubMed]

Charpak, S.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef] [PubMed]

Frenz, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

Genet, M.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Hu, S.

Jaeger, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

Jiao, S. L.

Laemmel, E.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Le Gargasson, J. F.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Le Goualher, G.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Lemor, R.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

Li, L.

Maslov, K.

Niederhauser, J. J.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

Oheim, M.

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef] [PubMed]

Perchant, A.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Puliafito, C. A.

Rao, B.

Shung, K. K.

R. J. Zemp, L. Song, R. Bitton, K. K. Shung, and L. V. Wang, Opt. Express 16, 7915 (2008).
[CrossRef] [PubMed]

L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
[CrossRef] [PubMed]

Song, L.

Stern, M. D.

M. D. Stern, Nature 254, 56 (1975).
[CrossRef] [PubMed]

Vicaut, E.

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Wang, L. V.

Wang, R. K.

Weber, P.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

Xie, Z. X.

Zemp, R. J.

Zhang, H. F.

IEEE Trans. Med. Imaging (1)

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, IEEE Trans. Med. Imaging 24, 436 (2005).
[CrossRef] [PubMed]

J Biomed. Opt. (2)

L. Song, K. Maslov, R. Bitton, K. K. Shung, and L. V. Wang, J Biomed. Opt. 13, 054028 (2008).
[CrossRef] [PubMed]

S. Hu and L. V. Wang, J Biomed. Opt. 15, 011101 (2010).
[CrossRef] [PubMed]

J. Vasc. Res. (1)

E. Laemmel, M. Genet, G. Le Goualher, A. Perchant, J. F. Le Gargasson, and E. Vicaut, J. Vasc. Res. 41, 400 (2004).
[CrossRef] [PubMed]

Nature (1)

M. D. Stern, Nature 254, 56 (1975).
[CrossRef] [PubMed]

Opt. Express (3)

Opt. Lett. (4)

Proc. Natl. Acad. Sci. USA (1)

E. Chaigneau, M. Oheim, E. Audinat, and S. Charpak, Proc. Natl. Acad. Sci. USA 100, 13081 (2003).
[CrossRef] [PubMed]

Other (1)

American National Standard for Safe Use of Lasers, Tech. Rep. “Standard for the safe use of lasers,” ANSI Z136.1-2000 (American National Standards Institute, 2000).

Supplementary Material (1)

» Media 1: MPG (3356 KB)     

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Figures (3)

Fig. 1
Fig. 1

Schematic of the multifocal OR-PAM system. The widths of the slit and the aperture along y are 50 μm and 5 mm , respectively.

Fig. 2
Fig. 2

Lateral resolution of the multifocal OR-PAM system. (a) Photoacoustic (PA) MAP image of two crossed 6 μm diameter carbon fibers. (b) Distribution of the PA amplitude (dots) along the dashed line in (a); the solid line is a Gaussian fitted curve.

Fig. 3
Fig. 3

In vivo photoacoustic image of a mouse ear microvasculature. (a) MAP image acquired at 570 nm . (b) Snapshot of a 3D animation (Media 1) showing the microvasculature in (a). (c) Close-up of the vasculature in the dashed box in (a). (d) Oxygen saturation ( sO 2 ) mapping for vessels in (c).

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