Abstract

Direct visualization of microvasculature provides significant insights in microcirculation and critically impacts the diagnosis and treatment of microcirculatory diseases. Recently, we discovered that the high-energy Soret fluorescence of hemoglobin peaked at 438nm with an extremely short lifetime becomes strongly visible under two-photon excitation. Based on the distinct spectral and temporal characteristics of hemoglobin fluorescence, we demonstrated that two-photon fluorescence microscopy could become a powerful tool for label-free in vivo imaging of microvasculature in tissue.

© 2011 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
Two-photon excited hemoglobin fluorescence

Wei Zheng, Dong Li, Yan Zeng, Yi Luo, and Jianan Y. Qu
Biomed. Opt. Express 2(1) 71-79 (2011)

Time-resolved detection enables standard two-photon fluorescence microscopy for in vivo label-free imaging of microvasculature in tissue

Dong Li, Wei Zheng, Wei Zhang, Seng Khoon Teh, Yan Zeng, Yi Luo, and Jianan Y. Qu
Opt. Lett. 36(14) 2638-2640 (2011)

In vivo micro-vascular imaging and flow cytometry in zebrafish using two-photon excited endogenous fluorescence

Yan Zeng, Bo Yan, Qiqi Sun, Sicong He, Jun Jiang, Zilong Wen, and Jianan Y. Qu
Biomed. Opt. Express 5(3) 653-663 (2014)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Supplementary Material (3)

» Media 1: AVI (868 KB)     
» Media 2: AVI (869 KB)     
» Media 3: AVI (2737 KB)     

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription