Abstract

We demonstrate rapid vibrational imaging based on sum frequency generation (SFG) microscopy with a collinear excitation geometry. Using the tunable picosecond pulses from a high-repetition-rate optical parametric oscillator, vibrationally selective imaging of collagen fibers is achieved with submicrometer lateral resolution. We furthermore show simultaneous SFG and second harmonic generation imaging to emphasize the compatibility of the microscope with other nonlinear optical modalities.

© 2011 Optical Society of America

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    [CrossRef]

2011 (2)

L. Fu, J. Liu, and E. C. S. Yan, J. Am. Chem. Soc. 133, 8094 (2011).
[CrossRef] [PubMed]

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

2010 (1)

2009 (3)

K. Locharoenrat, H. Sano, and G. Mizutani, Phys. Status Solidi C 6, 304 (2009).
[CrossRef]

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

2008 (1)

I. V. Stiopkin, H. D. Jayathilake, A. N. Bordenyuk, and A. V. Benderskii, J. Am. Chem. Soc. 130, 2271 (2008).
[CrossRef] [PubMed]

2007 (1)

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

2002 (3)

Z. Chen, Y. R. Shen, and G. A. Somorjai, Annu. Rev. Phys. Chem. 53, 437 (2002).
[CrossRef] [PubMed]

D. M. P. Hoffmann, K. Kuhnke, and K. Kern, Rev. Sci. Instrum. 73, 3221 (2002).
[CrossRef]

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

1999 (1)

M. Flörsheimer, C. Brillert, and H. Fuchs, Langmuir 15, 5437 (1999).
[CrossRef]

1989 (1)

Y. R. Shen, Nature 337, 519 (1989).
[CrossRef]

Baldelli, S.

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Benderskii, A. V.

I. V. Stiopkin, H. D. Jayathilake, A. N. Bordenyuk, and A. V. Benderskii, J. Am. Chem. Soc. 130, 2271 (2008).
[CrossRef] [PubMed]

Bordenyuk, A. N.

I. V. Stiopkin, H. D. Jayathilake, A. N. Bordenyuk, and A. V. Benderskii, J. Am. Chem. Soc. 130, 2271 (2008).
[CrossRef] [PubMed]

Boyd, R. W.

R. W. Boyd, Nonlinear Optics (Academic Press, 2003).

Brillert, C.

M. Flörsheimer, C. Brillert, and H. Fuchs, Langmuir 15, 5437 (1999).
[CrossRef]

Campagnola, P. J.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Chen, Z.

Z. Chen, Y. R. Shen, and G. A. Somorjai, Annu. Rev. Phys. Chem. 53, 437 (2002).
[CrossRef] [PubMed]

Chinwangso, P.

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Cimatu, K.

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Flörsheimer, M.

M. Flörsheimer, C. Brillert, and H. Fuchs, Langmuir 15, 5437 (1999).
[CrossRef]

Frank, C. W.

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

Fu, L.

L. Fu, J. Liu, and E. C. S. Yan, J. Am. Chem. Soc. 133, 8094 (2011).
[CrossRef] [PubMed]

Fuchs, H.

M. Flörsheimer, C. Brillert, and H. Fuchs, Langmuir 15, 5437 (1999).
[CrossRef]

Fujii, M.

Hernandez, M.

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Hoffmann, D. M. P.

D. M. P. Hoffmann, K. Kuhnke, and K. Kern, Rev. Sci. Instrum. 73, 3221 (2002).
[CrossRef]

Hoppe, P. E.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Inoue, K.

Jayathilake, H. D.

I. V. Stiopkin, H. D. Jayathilake, A. N. Bordenyuk, and A. V. Benderskii, J. Am. Chem. Soc. 130, 2271 (2008).
[CrossRef] [PubMed]

Kern, K.

D. M. P. Hoffmann, K. Kuhnke, and K. Kern, Rev. Sci. Instrum. 73, 3221 (2002).
[CrossRef]

Knoesen, A.

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

Kuhnke, K.

D. M. P. Hoffmann, K. Kuhnke, and K. Kern, Rev. Sci. Instrum. 73, 3221 (2002).
[CrossRef]

Lee, T. R.

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Liu, J.

L. Fu, J. Liu, and E. C. S. Yan, J. Am. Chem. Soc. 133, 8094 (2011).
[CrossRef] [PubMed]

Locharoenrat, K.

K. Locharoenrat, H. Sano, and G. Mizutani, Phys. Status Solidi C 6, 304 (2009).
[CrossRef]

Malone, C. J.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Matsumoto, Y.

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Mendoza, I. R.

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

Millard, A. C.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Miyauchi, Y.

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

Mizutani, G.

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

K. Locharoenrat, H. Sano, and G. Mizutani, Phys. Status Solidi C 6, 304 (2009).
[CrossRef]

Mohler, W. A.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Nakai, I. F.

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Okada, J.

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

Reiser, K. M.

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

Sakai, M.

Sano, H.

K. Locharoenrat, H. Sano, and G. Mizutani, Phys. Status Solidi C 6, 304 (2009).
[CrossRef]

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

Shen, Y. R.

Z. Chen, Y. R. Shen, and G. A. Somorjai, Annu. Rev. Phys. Chem. 53, 437 (2002).
[CrossRef] [PubMed]

Y. R. Shen, Nature 337, 519 (1989).
[CrossRef]

Somorjai, G. A.

Z. Chen, Y. R. Shen, and G. A. Somorjai, Annu. Rev. Phys. Chem. 53, 437 (2002).
[CrossRef] [PubMed]

Srisombat, L.

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Stiopkin, I. V.

I. V. Stiopkin, H. D. Jayathilake, A. N. Bordenyuk, and A. V. Benderskii, J. Am. Chem. Soc. 130, 2271 (2008).
[CrossRef] [PubMed]

Tachioka, M.

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Terasaki, M.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Ueda, T.

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Ugawa, A.

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Wang, M.

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

Watanabe, K.

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Yamashita, H.

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

Yan, E. C. S.

L. Fu, J. Liu, and E. C. S. Yan, J. Am. Chem. Soc. 133, 8094 (2011).
[CrossRef] [PubMed]

Yankelevich, D. R.

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

Annu. Rev. Phys. Chem. (1)

Z. Chen, Y. R. Shen, and G. A. Somorjai, Annu. Rev. Phys. Chem. 53, 437 (2002).
[CrossRef] [PubMed]

Appl. Phys. Lett. (1)

I. F. Nakai, M. Tachioka, A. Ugawa, T. Ueda, K. Watanabe, and Y. Matsumoto, Appl. Phys. Lett. 95, 243304 (2009).
[CrossRef]

Appl. Spectrosc. (1)

Biophys. J. (2)

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

I. R. Mendoza, D. R. Yankelevich, M. Wang, K. M. Reiser, C. W. Frank, and A. Knoesen, Biophys. J. 93, 4433 (2007).
[CrossRef]

J. Am. Chem. Soc. (2)

I. V. Stiopkin, H. D. Jayathilake, A. N. Bordenyuk, and A. V. Benderskii, J. Am. Chem. Soc. 130, 2271 (2008).
[CrossRef] [PubMed]

L. Fu, J. Liu, and E. C. S. Yan, J. Am. Chem. Soc. 133, 8094 (2011).
[CrossRef] [PubMed]

J. Phys. Chem. C (1)

M. Hernandez, P. Chinwangso, K. Cimatu, L. Srisombat, T. R. Lee, and S. Baldelli, J. Phys. Chem. C 115, 4688 (2011).
[CrossRef]

Langmuir (1)

M. Flörsheimer, C. Brillert, and H. Fuchs, Langmuir 15, 5437 (1999).
[CrossRef]

Nature (1)

Y. R. Shen, Nature 337, 519 (1989).
[CrossRef]

Phys. Status Solidi C (1)

K. Locharoenrat, H. Sano, and G. Mizutani, Phys. Status Solidi C 6, 304 (2009).
[CrossRef]

Rev. Sci. Instrum. (1)

D. M. P. Hoffmann, K. Kuhnke, and K. Kern, Rev. Sci. Instrum. 73, 3221 (2002).
[CrossRef]

Surf. Sci. (1)

Y. Miyauchi, H. Sano, J. Okada, H. Yamashita, and G. Mizutani, Surf. Sci. 603, 2972 (2009).
[CrossRef]

Other (1)

R. W. Boyd, Nonlinear Optics (Academic Press, 2003).

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Figures (4)

Fig. 1
Fig. 1

Experimental setup of VR-SFG microscope. The mid-IR and near-IR beams are combined and sent to an inverted microscope. The SFG and SHG signals are detected in the forward and epi directions, respectively. DC1, DC2, dichroic mirrors; M, metallic mirror; F, bandpass filters; COND, condenser; PMT, photomultiplier tube. The energy level diagrams for VR-SFG and SHG processes are shown in the figure.

Fig. 2
Fig. 2

(a) Power dependence of the SFG signal generated from a bulk GaP sample as a function of the mid-IR and near-IR power levels. The x and y axes are shown in log scale. (b) The SFG line scan of a 0.3 μm diameter barium titanate nanoparticle at focus. The inset shows the SFG image of a single nanoparticle.

Fig. 3
Fig. 3

Multimodal images of collagen from (a),(b) rat tail tendon and (c),(d) mouse kidney tissue section samples. (a),(c) VR-SFG image probing the 2945 cm 1 methylene mode and, (b),(d) SHG image of collagen. The scale bar is 20 μm .

Fig. 4
Fig. 4

Spectral scan images of collagen with the VR-SFG microscope. SFG images at (a) on-resonance, 2959 cm 1 , and (b) off-resonance, 2867 cm 1 . The scale bar is 20 μm .

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