Abstract

This Letter reports on the use of a supercontinuum light source to achieve ultrahigh resolution and ultrahigh sensitive optical microangiography (OMAG) imaging of microcirculations within tissue beds in vivo. After passing through a specially designed optical filter with a passband of 120nm centered on 800nm, the light source is coupled into an optic-fiber-based OMAG system that provides a measured axial resolution of 3μm over a ranging distance of 2mm. Within this ranging distance, the system gives an averaged signal-to-noise ratio of 87dB and a sensitivity roll-off of 7dB at an A-scan rate of 70kHz. We demonstrate the capability of the system to visualize a detailed microvascular perfusion map, including the single red blood cells within the capillaries, by imaging a mouse ear flap in vivo.

© 2011 Optical Society of America

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2010

2009

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1999

1991

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Adler, D.

An, L.

Apolonski, A.

Bajraszewski, T.

Bizheva, K.

Boppart, S. A.

Bornemann, J.

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Cimalla, P.

Cuevas, M.

Drexler, W.

Fercher, A.

Fercher, A. F.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Forst, M.

Fujimoto, J.

Fujimoto, J. G.

W. Drexler, U. Morgner, F. X. Kärtner, C. Pitris, S. A. Boppart, X. D. Li, E. P. Ippen, and J. G. Fujimoto, Opt. Lett. 24, 1221 (1999).
[CrossRef]

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Gruber, A.

Grychtol, P.

Hanson, S. R.

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hermann, B.

Hermes, B.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Hurst, S.

Ippen, E. P.

Jacques, S. L.

Jia, Y.

Jung, Y.

Kärtner, F. X.

Knight, J. C.

Ko, T.

Koch, E.

Kray, S.

Kurz, H.

Le, T.

Leitgeb, R.

Li, X. D.

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Ma, Z.

Mamedov, D.

Mehner, M.

Morgner, U.

Pitris, C.

Povazay, B.

Prokhorov, V.

Puliafito, C. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Qin, J.

Russell, P. St. J.

Sattmann, H.

Scherzer, E.

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Shidlovski, V.

Spoler, F.

Stingl, A.

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

Unterhuber, A.

Vetterlein, M.

Wadsworth, W. J.

Walther, J.

Wang, R. K.

Yakubovich, S.

Zhi, Z.

Biomed. Opt. Express

Opt. Express

Opt. Lett.

Science

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991).
[CrossRef] [PubMed]

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Figures (3)

Fig. 1
Fig. 1

Schematics of the system and characterization of its performance. (a) System setup, SD, spectral filter. (b) Sensitivity falling-off curve along the whole designed imaging depth and (c) the falling-off curve within the meaningful depth range of 2 mm [i.e., zoomed from the region outlined in (b)]. (d) Evaluated free-space axial resolution. Horizontal dashed line is the mean value.

Fig. 2
Fig. 2

(a) Cross-sectional structural image of mouse pinna in vivo: A, auricular cartilage; E, epidermis; H, hair follicle; F, fatty subcutaneous tissue; and (b) corresponding blood flow image. (c) 2D projection view of a 3D OMAG image of blood vessels within a 500 μm × 500 μm area and (d) a 3D view rendered by merging the structure with the blood vessels.

Fig. 3
Fig. 3

Whole-ear vascular network of a living mouse imaged by an SC light source coupled UHS-OMAG system. Insert is a picture of the mouse ear.

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