Abstract

Optical projection tomography is a new ex vivo imaging technique that allows imaging of whole organs in three dimensions at high spatial resolutions. In this Letter we demonstrate its capability to tomographically visualize molecular activity in whole organs of mice. In particular, eosinophil activity in asthmatic lungs is resolved using a Born-normalized fluorescence optical projection tomography and employing a near-IR molecular probe. The possibility to achieve molecularly sensitive imaging contrast in optical projection tomography by means of targeted and activatable imaging reporter agents adds a new range of capabilities for investigating molecular signatures of pathophysiological processes and a wide variety of diseases and their development.

© 2010 Optical Society of America

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  1. F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
    [CrossRef] [PubMed]
  2. F. Jaffer and R. Weissleder, Circ. Res. 94, 433 (2004).
    [CrossRef] [PubMed]
  3. R. Weissleder and C.-H. Tung, Nat. Biotechnol. 17, 375 (1999).
    [CrossRef] [PubMed]
  4. V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
    [CrossRef] [PubMed]
  5. C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
    [CrossRef]
  6. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
    [CrossRef]
  7. J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
    [CrossRef] [PubMed]
  8. C. Vinegoni, D. Razansky, J. Figuereido, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, Opt. Lett. 34, 319 (2009).
    [CrossRef] [PubMed]
  9. C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.
  10. C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
    [CrossRef]
  11. V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
    [CrossRef]

2009 (3)

2008 (2)

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

2007 (1)

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

2005 (1)

V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
[CrossRef] [PubMed]

2004 (1)

F. Jaffer and R. Weissleder, Circ. Res. 94, 433 (2004).
[CrossRef] [PubMed]

2002 (1)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

1999 (1)

R. Weissleder and C.-H. Tung, Nat. Biotechnol. 17, 375 (1999).
[CrossRef] [PubMed]

Ahlgren, U.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Aikawa, E.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Baldock, R.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Blois, J.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Davidson, D.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Distel, M.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Fexon, L.

C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
[CrossRef]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Figueiredo, J. L.

C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
[CrossRef]

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Figuereido, J.

Fumene Feruglio, P.

Hecksher-Sorensen, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Hill, B.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Jaffer, F.

F. Jaffer and R. Weissleder, Circ. Res. 94, 433 (2004).
[CrossRef] [PubMed]

Jaffer, F. A.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Josephson, L.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Kim, D. E.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Koester, R.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Kohler, R.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Kohler, R. H.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Libby, P.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Ma, R.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Nahrendorf, M.

C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
[CrossRef]

C. Vinegoni, D. Razansky, J. Figuereido, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, Opt. Lett. 34, 319 (2009).
[CrossRef] [PubMed]

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Newton, A. P.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Ntziachristos, V.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, D. Razansky, J. Figuereido, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, Opt. Lett. 34, 319 (2009).
[CrossRef] [PubMed]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
[CrossRef] [PubMed]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Pande, A. N.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Perrimon, N.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Perry, P.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Pitsouli, C.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Pittet, M. J.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Pivovarov, M.

C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
[CrossRef]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Pozzo, A.

Quinti, L.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Razansky, D.

C. Vinegoni, D. Razansky, J. Figuereido, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, Opt. Lett. 34, 319 (2009).
[CrossRef] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Retamozo, V.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Ripoll, J.

V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
[CrossRef] [PubMed]

Ross, A.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Sbarbati, A.

Sharpe, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Shi, G. P.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Smith, A.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Swirski, F. K.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Tung, C. H.

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

Tung, C. -H.

R. Weissleder and C.-H. Tung, Nat. Biotechnol. 17, 375 (1999).
[CrossRef] [PubMed]

Upadhyay, R.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Vinegoni, C.

C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
[CrossRef]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

C. Vinegoni, D. Razansky, J. Figuereido, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, Opt. Lett. 34, 319 (2009).
[CrossRef] [PubMed]

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Wang, L.

V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
[CrossRef] [PubMed]

Waterman, P.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Weissleder, R.

C. Vinegoni, L. Fexon, P. Fumene Feruglio, M. Pivovarov, J. L. Figueiredo, M. Nahrendorf, A. Pozzo, A. Sbarbati, and R. Weissleder, Opt. Express 17, 22320 (2009).
[CrossRef]

C. Vinegoni, D. Razansky, J. Figuereido, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, Opt. Lett. 34, 319 (2009).
[CrossRef] [PubMed]

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
[CrossRef] [PubMed]

F. Jaffer and R. Weissleder, Circ. Res. 94, 433 (2004).
[CrossRef] [PubMed]

R. Weissleder and C.-H. Tung, Nat. Biotechnol. 17, 375 (1999).
[CrossRef] [PubMed]

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

Yuan, H.

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Circ. Res. (1)

F. Jaffer and R. Weissleder, Circ. Res. 94, 433 (2004).
[CrossRef] [PubMed]

Circulation (1)

F. A. Jaffer, D. E. Kim, L. Quinti, C. H. Tung, E. Aikawa, A. N. Pande, R. H. Kohler, G. P. Shi, P. Libby, and R. Weissleder, Circulation 115, 2292 (2007).
[CrossRef] [PubMed]

J. Clin. Invest. (1)

V. Retamozo, F. K. Swirski, P. Waterman, H. Yuan, J. L. Figueiredo, A. P. Newton, R. Upadhyay, C. Vinegoni, R. Kohler, J. Blois, A. Smith, M. Nahrendorf, L. Josephson, R. Weissleder, and M. J. Pittet, J. Clin. Invest. 118, 4058 (2008).
[CrossRef]

Nat. Biotechnol. (2)

R. Weissleder and C.-H. Tung, Nat. Biotechnol. 17, 375 (1999).
[CrossRef] [PubMed]

V. Ntziachristos, J. Ripoll, L. Wang, and R. Weissleder, Nat. Biotechnol. 23, 313 (2005).
[CrossRef] [PubMed]

Nat. Methods (1)

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, Nat. Methods 5, 45 (2008).
[CrossRef]

Nat. Photonics (1)

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. Koester, and V. Ntziachristos, Nat. Photonics 3, 412 (2009).
[CrossRef]

Opt. Express (1)

Opt. Lett. (1)

Science (1)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, Science 296, 541 (2002).
[CrossRef] [PubMed]

Other (1)

C. Vinegoni, D. Razansky, J. L. Figueiredo, L. Fexon, M. Pivovarov, M. Nahrendorf, V. Ntziachristos, and R. Weissleder, JoVE 28, http://www.jove.com/index/details.stp?id=1389.

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Figures (3)

Fig. 1
Fig. 1

(a) Experimental setup: LS, light source; SH, shutter; D, diffuser; ExBPF, excitation wavelength filters; S, sample; EmBPF, fluorescence filters; TL, telecentric lens; CCD, imaging camera. (b) The imaged lung is held fixed in BABB solution embedded in an agarose gel cylinder. (c) After exciting the fluorophore located at position x, light at the emission wavelength λ fl is filtered and collected by the CCD’s pixel located at position x d using a telecentric lensing system.

Fig. 2
Fig. 2

Eosinophil-mediated MMP activity in OVA-challenged mice in dissected and chemically cleared lungs. (a) White light and (b) fluorescence reflectivity image of explanted inflamed lungs injected with MMPsense. (c) Fluorescence transillumination image of cleared sample. (d)–(f) Corresponding images for a control mouse without MMPsense administration. The white bar corresponds to 5 mm. Color map scale in arbitrary units. (b) and (e) as well as (c) and (f) are normalized on the same scale. (g) Hemotoxylin and eosin staining of an inflamed lung section (20×; black bar corresponds to 0.1 mm). (h) Intravital microscopy image evidencing collagen structures and eosinophils. Location of (g) and (h) is marked by the white box in (c).

Fig. 3
Fig. 3

Normalized Born fluorescence reconstructions of an OVA-challenged mouse that received MMPsense 680. (a) Three-dimensional lung reconstruction; (b)–(d) coronal, axial, and saggital sections. The distribution of the molecular imaging contrast agent correlates well with the presence of eosinophils, the source of MMP activity, in lung parenchyma and conducting airways. White bar, 5 mm.

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