Abstract

Imaging of cell nuclei plays a critical role in cancer diagnosis and prognosis. To image noninvasively cell nuclei in vivo without staining, we developed UV photoacoustic microscopy (UV-PAM), in which 266nm wavelength UV light excites unlabeled DNA and RNA in cell nuclei to produce photoacoustic waves. We applied UV-PAM to ex vivo imaging of cell nuclei in a mouse lip and a mouse small intestine and to in vivo imaging of the cell nuclei in the mouse skin. The UV-PAM images of unstained cell nuclei match the optical micrographs of the histologically stained cell nuclei. Given intrinsic optical contrast and high spatial resolution, in vivo label-free UV-PAM has potential for unique biological and clinical application.

© 2010 Optical Society of America

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  1. D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 4, 677 (2004).
    [CrossRef]
  2. D. W. Molavi, The Practice of Surgical Pathology: A Beginner’s Guide to the Diagnostic Process (Springer, 2008).
  3. S. Kaeb, M. Landthaler, and U. Hohenleutner, Lasers Med. Sci. 24, 819 (2009).
    [CrossRef]
  4. J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
    [CrossRef]
  5. J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
    [CrossRef]
  6. L. V. Wang, Nat. Photon. 3, 503 (2009).
    [CrossRef]
  7. S. Hu and L. V. Wang, J. Biomed. Opt. 15, 011101 (2010).
    [CrossRef]
  8. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
    [CrossRef]
  9. C. M. Stoscheck, Methods Enzymol. 182, 50 (1990).
    [CrossRef]
  10. J. McHowat, J. H. Jones, and M. H. Creer, J. Lipid Res. 37, 2450 (1996).

2010 (1)

S. Hu and L. V. Wang, J. Biomed. Opt. 15, 011101 (2010).
[CrossRef]

2009 (3)

S. Kaeb, M. Landthaler, and U. Hohenleutner, Lasers Med. Sci. 24, 819 (2009).
[CrossRef]

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

L. V. Wang, Nat. Photon. 3, 503 (2009).
[CrossRef]

2008 (2)

J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
[CrossRef]

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
[CrossRef]

2004 (1)

D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 4, 677 (2004).
[CrossRef]

1996 (1)

J. McHowat, J. H. Jones, and M. H. Creer, J. Lipid Res. 37, 2450 (1996).

1990 (1)

C. M. Stoscheck, Methods Enzymol. 182, 50 (1990).
[CrossRef]

Creer, M. H.

J. McHowat, J. H. Jones, and M. H. Creer, J. Lipid Res. 37, 2450 (1996).

Dusza, S. W.

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

Ericson, M. B.

J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
[CrossRef]

Fischer, A. H.

D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 4, 677 (2004).
[CrossRef]

Gareau, D. S.

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

Hohenleutner, U.

S. Kaeb, M. Landthaler, and U. Hohenleutner, Lasers Med. Sci. 24, 819 (2009).
[CrossRef]

Hu, S.

S. Hu and L. V. Wang, J. Biomed. Opt. 15, 011101 (2010).
[CrossRef]

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
[CrossRef]

Jones, J. H.

J. McHowat, J. H. Jones, and M. H. Creer, J. Lipid Res. 37, 2450 (1996).

Kaeb, S.

S. Kaeb, M. Landthaler, and U. Hohenleutner, Lasers Med. Sci. 24, 819 (2009).
[CrossRef]

Karen, J. K.

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

Landthaler, M.

S. Kaeb, M. Landthaler, and U. Hohenleutner, Lasers Med. Sci. 24, 819 (2009).
[CrossRef]

Maslov, K.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
[CrossRef]

McHowat, J.

J. McHowat, J. H. Jones, and M. H. Creer, J. Lipid Res. 37, 2450 (1996).

Molavi, D. W.

D. W. Molavi, The Practice of Surgical Pathology: A Beginner’s Guide to the Diagnostic Process (Springer, 2008).

Nehal, K. S.

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

Nickerson, J. A.

D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 4, 677 (2004).
[CrossRef]

Paoli, J.

J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
[CrossRef]

Rajadhyaksha, M.

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

Smedh, M.

J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
[CrossRef]

Stoscheck, C. M.

C. M. Stoscheck, Methods Enzymol. 182, 50 (1990).
[CrossRef]

Tudisco, M.

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

Wang, L. V.

S. Hu and L. V. Wang, J. Biomed. Opt. 15, 011101 (2010).
[CrossRef]

L. V. Wang, Nat. Photon. 3, 503 (2009).
[CrossRef]

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
[CrossRef]

Wennberg, A. M.

J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
[CrossRef]

Zhang, H. F.

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
[CrossRef]

Zink, D.

D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 4, 677 (2004).
[CrossRef]

Br. J. Dermatol. (1)

J. K. Karen, D. S. Gareau, S. W. Dusza, M. Tudisco, M. Rajadhyaksha, and K. S. Nehal, Br. J. Dermatol. 160, 1242(2009).
[CrossRef]

J. Biomed. Opt. (1)

S. Hu and L. V. Wang, J. Biomed. Opt. 15, 011101 (2010).
[CrossRef]

J. Invest. Dermatol. (1)

J. Paoli, M. Smedh, A. M. Wennberg, and M. B. Ericson, J. Invest. Dermatol. 128, 1248 (2008).
[CrossRef]

J. Lipid Res. (1)

J. McHowat, J. H. Jones, and M. H. Creer, J. Lipid Res. 37, 2450 (1996).

Lasers Med. Sci. (1)

S. Kaeb, M. Landthaler, and U. Hohenleutner, Lasers Med. Sci. 24, 819 (2009).
[CrossRef]

Methods Enzymol. (1)

C. M. Stoscheck, Methods Enzymol. 182, 50 (1990).
[CrossRef]

Nat. Photon. (1)

L. V. Wang, Nat. Photon. 3, 503 (2009).
[CrossRef]

Nat. Rev. Cancer (1)

D. Zink, A. H. Fischer, and J. A. Nickerson, Nat. Rev. Cancer 4, 677 (2004).
[CrossRef]

Proc. SPIE (1)

K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Proc. SPIE 6856, 68561I (2008).
[CrossRef]

Other (1)

D. W. Molavi, The Practice of Surgical Pathology: A Beginner’s Guide to the Diagnostic Process (Springer, 2008).

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