Abstract

Optoacoustic tomography can visualize optical contrast in tissues while capitalizing on the advantages of ultrasound, such as high spatial resolution and fast imaging capabilities. We report a novel multispectral optoacoustic tomography system for deep tissue small animal imaging. The previously undocumented capacity of whole-body optoacoustic tomography at a video rate has been demonstrated by visualizing mouse kidney perfusion using Indocyanine Green in vivo.

© 2010 Optical Society of America

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  1. V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
    [CrossRef]
  2. L. V. Wang, Med. Phys. 35, 5758 (2008).
    [CrossRef]
  3. D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
    [CrossRef]
  4. D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
    [CrossRef] [PubMed]
  5. R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
    [CrossRef] [PubMed]
  6. X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
    [CrossRef]
  7. S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
    [CrossRef] [PubMed]
  8. B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef] [PubMed]
  14. M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
    [PubMed]

2010

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

2009

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

2008

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

2007

2005

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

2004

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

2003

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

1976

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Aguirre, A.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Angelsen, B. A. J.

B. A. J. Angelsen, Ultrasound Imaging (Emantec AS, 2000).

Brecht, H. P.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Chen, Q.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Conjusteau, A.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Distel, M.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Ermilov, S. A.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Fronheiser, M.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Gamelin, J.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Guo, P. Y.

Huang, F.

Khamapirad, T.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Koster, R. W.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Ku, G.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Kwant, G.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Lacewell, R.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Landsman, M. L. J.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Leonard, M. H.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Li, C.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

Ma, R.

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Maurudis, A.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Mehta, K.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Miller, T.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

Mook, G. A.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

Ntziachristos, V.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

Oraevsky, A. A.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Pang, Y. J.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Perrimon, N.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Razansky, D.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

R. Ma, A. Taruttis, V. Ntziachristos, and D. Razansky, Opt. Express 17, 21414 (2009).
[CrossRef] [PubMed]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

Ripoll, J.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

Rosenthal, A.

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

Stoica, G.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Su, R.

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

Tan, Y.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Taruttis, A.

Vinegoni, C.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

D. Razansky, C. Vinegoni, and V. Ntziachristos, Opt. Lett. 32, 2891 (2007).
[CrossRef] [PubMed]

Wang, L. H. V.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Wang, L. V.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

Wang, X. D.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Wang, Y.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Weissleder, R.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

Xie, X. Y.

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Xing, D.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Yin, B. Z.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Zeng, Y. G.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Zhu, Q.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Y. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009).
[CrossRef] [PubMed]

Zijlstra, W. G.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

IEEE Trans. Med. Imaging

A. Rosenthal, D. Razansky, and V. Ntziachristos, IEEE Trans. Med. Imaging 28, 1997 (2009).
[CrossRef] [PubMed]

J. Appl. Physiol.

M. L. J. Landsman, G. Kwant, G. A. Mook, and W. G. Zijlstra, J. Appl. Physiol. 40, 575 (1976).
[PubMed]

J. Biomed. Opt.

S. A. Ermilov, T. Khamapirad, A. Conjusteau, M. H. Leonard, R. Lacewell, K. Mehta, T. Miller, and A. A. Oraevsky, J. Biomed. Opt. 14, 024007 (2009).
[CrossRef] [PubMed]

H. P. Brecht, R. Su, M. Fronheiser, S. A. Ermilov, A. Conjusteau, and A. A. Oraevsky, J. Biomed. Opt. 14, 064007 (2009).
[CrossRef]

J. Biomed. Opt. Lett.

C. Li, A. Aguirre, J. Gamelin, A. Maurudis, Q. Zhu, and L. V. Wang, J. Biomed. Opt. Lett. 15, 010509 (2010).
[CrossRef]

Med. Phys.

L. V. Wang, Med. Phys. 35, 5758 (2008).
[CrossRef]

Nat. Biotech.

V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, Nat. Biotech. 23, 313 (2005).
[CrossRef]

X. D. Wang, Y. J. Pang, G. Ku, X. Y. Xie, G. Stoica, and L. H. V. Wang, Nat. Biotech. 21, 803 (2003).
[CrossRef]

Nat. Photon.

D. Razansky, M. Distel, C. Vinegoni, R. Ma, N. Perrimon, R. W. Koster, and V. Ntziachristos, Nat. Photon. 3, 412(2009).
[CrossRef]

Opt. Express

Opt. Lett.

Phys. Med. Biol.

B. Z. Yin, D. Xing, Y. Wang, Y. G. Zeng, Y. G. Zeng, Y. Tan, and Q. Chen, Phys. Med. Biol. 49, 1339 (2004).
[CrossRef] [PubMed]

Other

B. A. J. Angelsen, Ultrasound Imaging (Emantec AS, 2000).

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Figures (4)

Fig. 1
Fig. 1

(a) Schematic representation of the MSOT system. A curved array of wideband and cylindrically focused ultrasound transducers enables parallel data acquisition. Optical fibers are used to homogeneously illuminate the object. A special animal holder with a transparent plastic membrane is used for animal positioning. (b) Picture of the mouse during a scan, showing the position of the mouse and illumination with respect to the array of focused wideband ultrasound transducers.

Fig. 2
Fig. 2

(a) Stack of representative slices of a 3D dataset of the pelvis and kidney region of a female eight-week-old CD1 mouse. (b) Cross-sectional optoacoustic image before injection of the slice in which the kinetics of ICG was monitored. (c) Photograph of a cross-sectional cryoslice at a height corresponding to that shown in (b): 1, vena cava; 2, portal vein; 3, kidneys; 4, spinal cord; 5, backbone muscles; and 6, spleen.

Fig. 3
Fig. 3

(a) Cross-sectional optoacoustic images at different time points of the kidneys of a female CD1 mouse illuminated at 800 nm after having injected 300 nmol of ICG. (b) Superposition of the absorption difference and the single- wavelength image before injection.

Fig. 4
Fig. 4

(a) Superposition of a single wavelength image ( 890 nm ) and the unmixed component corresponding to the ICG signal. (b) Molar extinction coefficient of ICG [14], oxygenized and deoxygenized hemoglobin [4].

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