Abstract

We develop light-driven optoelectronic tweezers based on the organic photoconductive material titanium oxide phthalocyanine. These tweezers function based on negative dielectrophoresis (nDEP). The dynamic manipulation of a single microparticle and cell patterning are demonstrated by using this light-driven optoelectronic DEP chip. The adaptive light patterns that drive the optoelectronic DEP onchip are designed by using Flash software to approach appropriate dynamic manipulation. This is also the first reported demonstration, to the best of our knowledge, for successfully patterning such delicate cells from human hepatocellular liver carcinoma cell line HepG2 by using any optoelectronic tweezers.

© 2010 Optical Society of America

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    [CrossRef]

2010

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

2009

2007

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

W. Choi, S. H. Kim, J. Jang, and J. K. Park, Microfluid. Nanofluid. 3, 217 (2007).
[CrossRef]

W. B. Wang, X. G. Li, S. R. Wang, and W. Hou, Dyes Pigm. 72, 38 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

2006

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

2005

P. Y. Chiou, A. T. Ohta, and M. C. Wu, Nature 436, 370 (2005).
[CrossRef] [PubMed]

2003

T. B. Jones, IEEE Eng. Med. Biol. Mag. 22, 33 (2003).
[CrossRef]

2000

C. J. Lee, J. H. Park, and J. Park, Chem. Phys. Lett. 323, 560 (2000).
[CrossRef]

1996

K. Ogawa, J. Yao, H. Yonehara, and C. Pac, J. Mater. Chem. 6, 143 (1996).
[CrossRef]

R. Pethig, Crit. Rev. Biotechnol. 16, 331 (1996).
[CrossRef]

1993

K. Y. Law, Chem. Rev. 93, 449 (1993).
[CrossRef]

1987

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

Ashkin, A.

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

Bhardwaj, U.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Chang, H. Y.

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

Chang, W. Y.

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

Chiou, P. Y.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, Nature 436, 370 (2005).
[CrossRef] [PubMed]

Chiou, P.-Y.

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

Choi, W.

W. Choi, S. H. Kim, J. Jang, and J. K. Park, Microfluid. Nanofluid. 3, 217 (2007).
[CrossRef]

Dziedzic, J. M.

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

Fuqu, Y.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Guan, R.-S.

Guo, T.-F.

Han, T. H.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Ho, C. T.

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

Hou, W.

W. B. Wang, X. G. Li, S. R. Wang, and W. Hou, Dyes Pigm. 72, 38 (2007).
[CrossRef]

Hsu, H. Y.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

Hsu, H.-Y.

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

Jamshidi, A.

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

Jang, J.

W. Choi, S. H. Kim, J. Jang, and J. K. Park, Microfluid. Nanofluid. 3, 217 (2007).
[CrossRef]

Jones, T. B.

T. B. Jones, IEEE Eng. Med. Biol. Mag. 22, 33 (2003).
[CrossRef]

Kim, S. H.

W. Choi, S. H. Kim, J. Jang, and J. K. Park, Microfluid. Nanofluid. 3, 217 (2007).
[CrossRef]

Lau, A. N. K.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

Law, K. Y.

K. Y. Law, Chem. Rev. 93, 449 (1993).
[CrossRef]

Lee, C. J.

C. J. Lee, J. H. Park, and J. Park, Chem. Phys. Lett. 323, 560 (2000).
[CrossRef]

Lee, G.-B.

Li, X. G.

W. B. Wang, X. G. Li, S. R. Wang, and W. Hou, Dyes Pigm. 72, 38 (2007).
[CrossRef]

Liao, J. C.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Lin, R. Z.

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

Lin, Ye.-H.

Liu, C. H.

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

McCabe, E. R. B.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Neale, S. L.

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

Ogawa, K.

K. Ogawa, J. Yao, H. Yonehara, and C. Pac, J. Mater. Chem. 6, 143 (1996).
[CrossRef]

Ohta, A. T.

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, Nature 436, 370 (2005).
[CrossRef] [PubMed]

Pac, C.

K. Ogawa, J. Yao, H. Yonehara, and C. Pac, J. Mater. Chem. 6, 143 (1996).
[CrossRef]

Park, J.

C. J. Lee, J. H. Park, and J. Park, Chem. Phys. Lett. 323, 560 (2000).
[CrossRef]

Park, J. H.

C. J. Lee, J. H. Park, and J. Park, Chem. Phys. Lett. 323, 560 (2000).
[CrossRef]

Park, J. K.

W. Choi, S. H. Kim, J. Jang, and J. K. Park, Microfluid. Nanofluid. 3, 217 (2007).
[CrossRef]

Pethig, R.

R. Pethig, Crit. Rev. Biotechnol. 16, 331 (1996).
[CrossRef]

Phan, H. L.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

Ren, S.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Sherwood, S. W.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

Wang, S. R.

W. B. Wang, X. G. Li, S. R. Wang, and W. Hou, Dyes Pigm. 72, 38 (2007).
[CrossRef]

Wang, W.

Wang, W. B.

W. B. Wang, X. G. Li, S. R. Wang, and W. Hou, Dyes Pigm. 72, 38 (2007).
[CrossRef]

Wen, T.-C.

Wu, M. C.

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, Nature 436, 370 (2005).
[CrossRef] [PubMed]

Yamane, T.

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

Yang, J. M.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

Yao, J.

K. Ogawa, J. Yao, H. Yonehara, and C. Pac, J. Mater. Chem. 6, 143 (1996).
[CrossRef]

Yonehara, H.

K. Ogawa, J. Yao, H. Yonehara, and C. Pac, J. Mater. Chem. 6, 143 (1996).
[CrossRef]

Chem. Phys. Lett.

C. J. Lee, J. H. Park, and J. Park, Chem. Phys. Lett. 323, 560 (2000).
[CrossRef]

Chem. Rev.

K. Y. Law, Chem. Rev. 93, 449 (1993).
[CrossRef]

Crit. Rev. Biotechnol.

R. Pethig, Crit. Rev. Biotechnol. 16, 331 (1996).
[CrossRef]

Dyes Pigm.

W. B. Wang, X. G. Li, S. R. Wang, and W. Hou, Dyes Pigm. 72, 38 (2007).
[CrossRef]

IEEE Eng. Med. Biol. Mag.

T. B. Jones, IEEE Eng. Med. Biol. Mag. 22, 33 (2003).
[CrossRef]

IEEE J. Sel. Top. Quantum Electron.

A. T. Ohta, P. Y. Chiou, H. L. Phan, S. W. Sherwood, J. M. Yang, A. N. K. Lau, H. Y. Hsu, A. Jamshidi, and M. C. Wu, IEEE J. Sel. Top. Quantum Electron. 13, 235 (2007).
[CrossRef]

J. Mater. Chem.

K. Ogawa, J. Yao, H. Yonehara, and C. Pac, J. Mater. Chem. 6, 143 (1996).
[CrossRef]

J. Microelectromech. Syst.

A. T. Ohta, P. Y. Chiou, T. H. Han, J. C. Liao, U. Bhardwaj, E. R. B. McCabe, Y. Fuqu, S. Ren, and M. C. Wu, J. Microelectromech. Syst. 16, 491 (2007).
[CrossRef]

Lab Chip

C. T. Ho, R. Z. Lin, W. Y. Chang, H. Y. Chang, and C. H. Liu, Lab Chip 6, 724 (2006).
[CrossRef] [PubMed]

H.-Y. Hsu, A. T. Ohta, P.-Y. Chiou, A. Jamshidi, S. L. Neale, and M. C. Wu, Lab Chip 10, 165 (2010).
[CrossRef] [PubMed]

Microfluid. Nanofluid.

W. Choi, S. H. Kim, J. Jang, and J. K. Park, Microfluid. Nanofluid. 3, 217 (2007).
[CrossRef]

Nature

A. Ashkin, J. M. Dziedzic, and T. Yamane, Nature 330, 769 (1987).
[CrossRef] [PubMed]

P. Y. Chiou, A. T. Ohta, and M. C. Wu, Nature 436, 370 (2005).
[CrossRef] [PubMed]

Opt. Express

Supplementary Material (2)

» Media 1: MOV (3951 KB)     
» Media 2: MOV (3853 KB)     

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Figures (5)

Fig. 1
Fig. 1

Operation principle of Ti-OET and simulation of the light-induced electric field.

Fig. 2
Fig. 2

Schematic diagram of the optical system setup for the light-driven optoelectronic tweezers.

Fig. 3
Fig. 3

(a) Beads are captured by the light- induced ring-shaped nDEP clamp. (b) The trapped beads are transported by following the arrow path to the entrance of the house-shaped light pattern. (c) and (d) The light-induced nDEP putter pushes the beads leftward. All the demonstrations are operated via Flash codes and cursor action. The scale bar is 100 μm (Media 1).

Fig. 4
Fig. 4

(a)–(c) Real-time image recording for our liver-cell patterning demonstration by using light-driven optoelectronic manipulation. (a) and (d) Fluorescent microscope images for on-chip light-induced cell patterning in the beginning and after 10 min , respectively. The scale bar is 100 μm . (Media 2 is demonstrated by using latex beads instead of cells for a more visible demonstration).

Fig. 5
Fig. 5

Characterized relationship between the light-induced DEP force and the applied voltage at 20 kHz for 15 μm polymer beads on our Ti-OET chip.

Equations (1)

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F Ti-OET = 2 π r ε m Re [ f CM ( ω ) ] E rms 2 ,

Metrics