Abstract

Focal modulation microscopy is an emerging fluorescence microscopy technique for in vivo imaging of thick biological tissues. Here, we present a theoretical study to assess its performance. The scalar diffraction theory is combined with Monte Carlo simulation to evaluate the signal-to-background ratio at various depths. The performance of confocal microscopy with a similar optical setup is also evaluated for comparison.

© 2010 Optical Society of America

Full Article  |  PDF Article
OSA Recommended Articles
High-speed focal modulation microscopy using acousto-optical modulators

Shau Poh Chong, Chee Howe Wong, Kit Fei Wong, Colin J.R. Sheppard, and Nanguang Chen
Biomed. Opt. Express 1(3) 1026-1037 (2010)

Numerical studies of focal modulation microscopy in high-NA system

Bingzhao Zhu, Shuhao Shen, Yao Zheng, Wei Gong, and Ke Si
Opt. Express 24(17) 19138-19147 (2016)

Considerations of aperture configuration in focal modulation microscopy from the standpoint of modulation depth

Guangjun Gao, Shau Poh Chong, Colin J. R. Sheppard, and Nanguang Chen
J. Opt. Soc. Am. A 28(4) 496-501 (2011)

References

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Equations (8)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription

Metrics

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access OSA Member Subscription