Abstract

Capillaries, the smallest blood vessels, are the distal end of the vasculature where oxygen and nutrients are exchanged between blood and tissue. Hence, noninvasive imaging of capillaries and function in vivo has long been desired as a window to studying fundamental physiology, such as neurovascular coupling. Existing imaging modalities cannot provide the required sensitivity and spatial resolution. We present in vivo imaging of the microvasculature including single capillaries in mice using optical-resolution photoacoustic microscopy (OR-PAM) developed in our laboratory. OR-PAM provides a lateral resolution of 5 μm and an imaging depth >0.7 mm.

© 2008 Optical Society of America

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  1. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
    [CrossRef]
  2. A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.
  3. K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
    [CrossRef]
  4. H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).
  5. A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).
  6. S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).
  7. D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).
  8. V. P. Zharov, E. I. Galanzha, E. V. Shashkov, N. G. Khlebtsov, and V. V. Tuchin, Opt. Lett. 31, 3623 (2006).
    [CrossRef]
  9. Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2000 (American National Standards Institute, Inc., 2000).

2007 (1)

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).

2006 (2)

2005 (1)

2001 (2)

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Arbeit, J. M.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Basu, S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Bliss, V. S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Dasgupta, P. S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Dovrak, H. F.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Eckelhoefer, I. A.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Elson, D. A.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Galanzha, E. I.

Ginzinger, D. G.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Guyton, A. C.

A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).

Hall, J. E.

A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).

Huang, L. E.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Johnson, R. S.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Karabutov, A. A.

A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Khlebtsov, N. G.

Manseau, E. J.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Maslov, K.

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
[CrossRef]

McDonald, D. M.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Mukhopadhyay, D.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Nagy, J. A.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Oraevsky, A. A.

A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Pal, S.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Shashkov, E. V.

Stoica, G.

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
[CrossRef]

Thurston, G.

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Tuchin, V. V.

Vasile, E.

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Wang, L. V.

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

K. Maslov, G. Stoica, and L. V. Wang, Opt. Lett. 30, 625 (2005).
[CrossRef]

Zhang, H. F.

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Zharov, V. P.

Genes Dev. (1)

D. A. Elson, G. Thurston, L. E. Huang, D. G. Ginzinger, D. M. McDonald, R. S. Johnson, and J. M. Arbeit, Genes Dev. 15, 2520 (2001).

Nat. Biotechnol. (1)

H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotechnol. 24, 848 (2006).
[CrossRef]

Nat. Med. (1)

S. Basu, J. A. Nagy, S. Pal, E. Vasile, I. A. Eckelhoefer, V. S. Bliss, E. J. Manseau, P. S. Dasgupta, H. F. Dovrak, and D. Mukhopadhyay, Nat. Med. 7, 569 (2001).

Nat. Protocols (1)

H. F. Zhang, K. Maslov, and L. V. Wang, Nat. Protocols 4, 797 (2007).

Opt. Lett. (2)

Other (3)

A. C. Guyton and J. E. Hall, Textbook of Medical Physiology (Saunders, 2000).

A. A. Oraevsky and A. A. Karabutov, “Optoacoustic tomography,” in Biomedical Photonics Handbook, T.Vo-Dinh, ed. (CRC Press, 2003), Vol. PM125, Chap. 34.

Laser Institute of America, American National Standard for Safe Use of Lasers ANSI Z136.1-2000 (American National Standards Institute, Inc., 2000).

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