Abstract

Using femtosecond laser writing, optical waveguides were monolithically integrated into a commercial microfluidic lab-on-a-chip device, with the waveguides intersecting a microfluidic channel. Continuous-wave laser excitation through these optical waveguides confines the excitation window to a width of 12μm, enabling high-resolution monitoring of the passage of different types of fluorescent analytes when migrating and being separated in the microfluidic channel by microchip capillary electrophoresis. Furthermore, we demonstrate on-chip-integrated waveguide excitation and detection of a biologically relevant species, fluorescently labeled DNA molecules, during microchip capillary electrophoresis. Well-controlled plug formation as required for on-chip integrated capillary electrophoresis separation of DNA molecules, and the combination of waveguide excitation and a low limit of detection, will enable monitoring of extremely small quantities with high spatial resolution.

© 2008 Optical Society of America

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References

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  1. A. Manz, N. Graber, and H. M. Widmer, Sens. Actuators B 1, 244 (1990).
    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]
  9. R. Osellame, S. Taccheo, M. Marangoni, R. Ramponi, P. Laporta, D. Polli, S. De Silvestri, and G. Cerullo, J. Opt. Soc. Am. B 20, 1559 (2003).
    [CrossRef]
  10. G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
    [CrossRef] [PubMed]

2008

R. R. Gattass and E. Mazur, Nat. Photonics 2, 219 (2008).
[CrossRef]

2007

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

B. Kuswandi, Nuriman, J. Huskens, and W. Verboom, Anal. Chim. Acta 601, 141 (2007).
[CrossRef] [PubMed]

2006

P. V. Lambeck, Meas. Sci. Technol. 17, 93 (2006).
[CrossRef]

2003

J. P. Landers, Anal. Chem. 75, 2919 (2003).
[CrossRef] [PubMed]

R. Osellame, S. Taccheo, M. Marangoni, R. Ramponi, P. Laporta, D. Polli, S. De Silvestri, and G. Cerullo, J. Opt. Soc. Am. B 20, 1559 (2003).
[CrossRef]

S. Nolte, M. Will, J. Burghoff, and A. Tünnermann, Appl. Phys. A 77, 109 (2003).
[CrossRef]

2000

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

1990

A. Manz, N. Graber, and H. M. Widmer, Sens. Actuators B 1, 244 (1990).
[CrossRef]

Attiya, S.

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

Burghoff, J.

S. Nolte, M. Will, J. Burghoff, and A. Tünnermann, Appl. Phys. A 77, 109 (2003).
[CrossRef]

Cerullo, G.

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

R. Osellame, S. Taccheo, M. Marangoni, R. Ramponi, P. Laporta, D. Polli, S. De Silvestri, and G. Cerullo, J. Opt. Soc. Am. B 20, 1559 (2003).
[CrossRef]

De Silvestri, S.

Gattass, R. R.

R. R. Gattass and E. Mazur, Nat. Photonics 2, 219 (2008).
[CrossRef]

Graber, N.

A. Manz, N. Graber, and H. M. Widmer, Sens. Actuators B 1, 244 (1990).
[CrossRef]

Harrison, D. J.

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

Huskens, J.

B. Kuswandi, Nuriman, J. Huskens, and W. Verboom, Anal. Chim. Acta 601, 141 (2007).
[CrossRef] [PubMed]

Jiang, G.

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

Kuswandi, B.

B. Kuswandi, Nuriman, J. Huskens, and W. Verboom, Anal. Chim. Acta 601, 141 (2007).
[CrossRef] [PubMed]

Lambeck, P. V.

P. V. Lambeck, Meas. Sci. Technol. 17, 93 (2006).
[CrossRef]

Landers, J. P.

J. P. Landers, Anal. Chem. 75, 2919 (2003).
[CrossRef] [PubMed]

Laporta, P.

Lee, W. E.

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

Manz, A.

A. Manz, N. Graber, and H. M. Widmer, Sens. Actuators B 1, 244 (1990).
[CrossRef]

Marangoni, M.

Martinez-Vazquez, R.

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

Maselli, V.

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

Mazur, E.

R. R. Gattass and E. Mazur, Nat. Photonics 2, 219 (2008).
[CrossRef]

Nolte, S.

S. Nolte, M. Will, J. Burghoff, and A. Tünnermann, Appl. Phys. A 77, 109 (2003).
[CrossRef]

Nuriman,

B. Kuswandi, Nuriman, J. Huskens, and W. Verboom, Anal. Chim. Acta 601, 141 (2007).
[CrossRef] [PubMed]

Ocvirk, G.

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

Osellame, R.

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

R. Osellame, S. Taccheo, M. Marangoni, R. Ramponi, P. Laporta, D. Polli, S. De Silvestri, and G. Cerullo, J. Opt. Soc. Am. B 20, 1559 (2003).
[CrossRef]

Polli, D.

Ramponi, R.

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

R. Osellame, S. Taccheo, M. Marangoni, R. Ramponi, P. Laporta, D. Polli, S. De Silvestri, and G. Cerullo, J. Opt. Soc. Am. B 20, 1559 (2003).
[CrossRef]

Taccheo, S.

Tünnermann, A.

S. Nolte, M. Will, J. Burghoff, and A. Tünnermann, Appl. Phys. A 77, 109 (2003).
[CrossRef]

Verboom, W.

B. Kuswandi, Nuriman, J. Huskens, and W. Verboom, Anal. Chim. Acta 601, 141 (2007).
[CrossRef] [PubMed]

Westbrook, C. A.

C. A. Westbrook, U.S. patent No. 6,025,126 (2000).

Widmer, H. M.

A. Manz, N. Graber, and H. M. Widmer, Sens. Actuators B 1, 244 (1990).
[CrossRef]

Will, M.

S. Nolte, M. Will, J. Burghoff, and A. Tünnermann, Appl. Phys. A 77, 109 (2003).
[CrossRef]

Anal. Chem.

J. P. Landers, Anal. Chem. 75, 2919 (2003).
[CrossRef] [PubMed]

Anal. Chim. Acta

B. Kuswandi, Nuriman, J. Huskens, and W. Verboom, Anal. Chim. Acta 601, 141 (2007).
[CrossRef] [PubMed]

Appl. Phys. A

S. Nolte, M. Will, J. Burghoff, and A. Tünnermann, Appl. Phys. A 77, 109 (2003).
[CrossRef]

Appl. Phys. Lett.

R. Osellame, V. Maselli, R. Martinez-Vazquez, R. Ramponi, and G. Cerullo, Appl. Phys. Lett. 90, 231118 (2007).
[CrossRef]

Biosens. Bioelectron.

G. Jiang, S. Attiya, G. Ocvirk, W. E. Lee, and D. J. Harrison, Biosens. Bioelectron. 14, 861 (2000).
[CrossRef] [PubMed]

J. Opt. Soc. Am. B

Meas. Sci. Technol.

P. V. Lambeck, Meas. Sci. Technol. 17, 93 (2006).
[CrossRef]

Nat. Photonics

R. R. Gattass and E. Mazur, Nat. Photonics 2, 219 (2008).
[CrossRef]

Sens. Actuators B

A. Manz, N. Graber, and H. M. Widmer, Sens. Actuators B 1, 244 (1990).
[CrossRef]

Other

C. A. Westbrook, U.S. patent No. 6,025,126 (2000).

Supplementary Material (2)

» Media 1: MOV (1782 KB)     
» Media 2: MOV (562 KB)     

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Figures (3)

Fig. 1
Fig. 1

Layout of the MF chip with an integrated optical WG and schematic of the MF chip showing the passage of a DNA plug being monitored by the integrated WG.

Fig. 2
Fig. 2

Passage in a MF channel, and across an excitation WG, of (a) fluorescently labeled DNA (Media 1) and (b) fluorescent dyes Rhodamine-6G and Rhodamine-B (Media 2).

Fig. 3
Fig. 3

Time-dependent fluorescence intensity at the WG-MF-channel intersection after injection into the separation channel for a sample containing (a) fluorescently labeled DNA molecules (amplicon and template) or (b) fluorescent dyes Rhodamine-6G and Rhodamine-B.

Metrics