Abstract

We perform field-based angle-resolved light-scattering measurements from single live cells. We use a laser interferometer to acquire phase and amplitude images of cells at the image plane. The angular scattering spectrum is calculated from the Fourier transform of the field transmitted through the cells. A concurrent 3D refractive index distribution of the same cells is measured using tomographic phase microscopy. By measuring transient increases in light scattering by single cells during exposure to acetic acid, we correlate the scattering properties of single cells with their refractive index distributions and show that results are in good agreement with a model based on the Born approximation.

© 2008 Optical Society of America

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    [CrossRef]

2008 (1)

2007 (3)

T. T. Wu, J. Y. Qu, and M. Xu, Opt. Lett. 32, 2324 (2007).
[CrossRef] [PubMed]

J. D. Wilson and T. H. Foster, J. Biomed. Opt. 12, 030503 (2007).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

2006 (1)

1999 (2)

K. Sokolov, R. Drezek, K. Gossage, and R. Richards-Kortum, Opt. Express 5, 302 (1999).
[CrossRef] [PubMed]

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

1998 (1)

1988 (1)

K. Creath, Prog. Opt. 26, 349 (1988).
[CrossRef]

1969 (1)

E. Wolf, Opt. Commun. 1, 153 (1969).
[CrossRef]

Abbas, A. K.

V. Kumar, A. K. Abbas, and N. Fausto, Robbins & Cotran Pathologic Basis of Disease, 7th ed. (2005).

Backman, V.

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Badizadegan, K.

W. Choi, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 33, 171 (2008).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

C. C. Yu, C. Lau, J. W. Tunnell, M. Hunter, M. Kalashnikov, C. Fang-Yen, S. F. Fulghum, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 3119 (2006).
[CrossRef] [PubMed]

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Choi, W.

W. Choi, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 33, 171 (2008).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Creath, K.

K. Creath, Prog. Opt. 26, 349 (1988).
[CrossRef]

Dasari, R. R.

W. Choi, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 33, 171 (2008).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

C. C. Yu, C. Lau, J. W. Tunnell, M. Hunter, M. Kalashnikov, C. Fang-Yen, S. F. Fulghum, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 3119 (2006).
[CrossRef] [PubMed]

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Drezek, R.

Eick, A. A.

Fang-Yen, C.

Fausto, N.

V. Kumar, A. K. Abbas, and N. Fausto, Robbins & Cotran Pathologic Basis of Disease, 7th ed. (2005).

Feld, M. S.

W. Choi, C. Fang-Yen, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 33, 171 (2008).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

C. C. Yu, C. Lau, J. W. Tunnell, M. Hunter, M. Kalashnikov, C. Fang-Yen, S. F. Fulghum, K. Badizadegan, R. R. Dasari, and M. S. Feld, Opt. Lett. 31, 3119 (2006).
[CrossRef] [PubMed]

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Foster, T. H.

J. D. Wilson and T. H. Foster, J. Biomed. Opt. 12, 030503 (2007).
[CrossRef] [PubMed]

Freyer, J. P.

Fulghum, S. F.

Gossage, K.

Gurjar, R.

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Hielscher, A. H.

Hunter, M.

Itzkan, L.

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Johnson, T. M.

Kak, A. C.

A. C. Kak, and M. Slaney, Principles of Computerized Tomographic Imaging (Academic, New York, 1999).

Kalashnikov, M.

Kumar, V.

V. Kumar, A. K. Abbas, and N. Fausto, Robbins & Cotran Pathologic Basis of Disease, 7th ed. (2005).

Lau, C.

Lue, N.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Mourant, J. R.

Oh, S.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Perelman, L. T.

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

Qu, J. Y.

Richards-Kortum, R.

Shen, D.

Slaney, M.

A. C. Kak, and M. Slaney, Principles of Computerized Tomographic Imaging (Academic, New York, 1999).

Sokolov, K.

Tunnell, J. W.

Wilson, J. D.

J. D. Wilson and T. H. Foster, J. Biomed. Opt. 12, 030503 (2007).
[CrossRef] [PubMed]

Wolf, E.

E. Wolf, Opt. Commun. 1, 153 (1969).
[CrossRef]

Wu, T. T.

Xu, M.

Yu, C. C.

Appl. Opt. (1)

IEEE J. Sel. Top. Quantum Electron. (1)

V. Backman, R. Gurjar, K. Badizadegan, L. Itzkan, R. R. Dasari, L. T. Perelman, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 5, 1019 (1999).
[CrossRef]

J. Biomed. Opt. (1)

J. D. Wilson and T. H. Foster, J. Biomed. Opt. 12, 030503 (2007).
[CrossRef] [PubMed]

Nat. Methods (1)

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, Nat. Methods 4, 717 (2007).
[CrossRef] [PubMed]

Opt. Commun. (1)

E. Wolf, Opt. Commun. 1, 153 (1969).
[CrossRef]

Opt. Express (1)

Opt. Lett. (3)

Prog. Opt. (1)

K. Creath, Prog. Opt. 26, 349 (1988).
[CrossRef]

Other (2)

A. C. Kak, and M. Slaney, Principles of Computerized Tomographic Imaging (Academic, New York, 1999).

V. Kumar, A. K. Abbas, and N. Fausto, Robbins & Cotran Pathologic Basis of Disease, 7th ed. (2005).

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Figures (3)

Fig. 1
Fig. 1

Schematic diagram of tomographic phase microscope: GM, galvanometer scanning mirror; L1, focal length f = 250 mm lens; BF, backfocal plane of the condenser lens; C, condenser lens; S, sample; OL, objective lens; L2, f = 200 mm lens; AOM1 and 2, acousto-optic modulators; BS1 and 2, beam splitters. The frequency-shifted reference laser beam is shown as darkned after AOM.

Fig. 2
Fig. 2

Angular distribution of light scattering for a 10 μ m polystyrene bead: (a) amplitude image of the bead, (b) quantitative phase image of the bead (the scale bar indicates 10 μ m , and the color bar indicates the phase in radians); (c) angular scattering distribution, E ̃ ( ν x , ν y ) 2 (the 1 μ m 1 scale indicates the base-10 logarithm of the intensity); and (d) angular scattering distribution obtained from Mie theory (dashed-dotted curve), tomogram (dotted curve) and field image (solid curve), respectively.

Fig. 3
Fig. 3

Angular-scattering distribution for a live HeLa cell: (a) refractive index tomograms in normal culture medium; (b) medium containing 0.5% acetic acid and (c) after replacing the original culture medium; (d) angular-scattering distributions, I s ( σ ) , measured from the field image under three different conditions; and (e) angular-scattering distributions calculated from refractive index tomograms [(a)–(c)] measured by TPM.

Equations (1)

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E ̃ s ( ν x , ν y ) = π i ν 0 2 ν x 2 ν y 2 O ̃ ( ν x , ν y , ν 0 2 ν x 2 ν y 2 ν 0 ) ,

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