Abstract

We comment on a Letter by Toytman et al. [Opt. Lett. 32, 1941 (2007)] in which a novel setup for wide-field imaging in coherent anti-Stokes Raman scattering (CARS) microscopy is demonstrated. There the authors state that our phase-matching implementation of a wide-field CARS system [Appl. Phys. Lett. 84, 816 (2004) ; New J. Phys. 8, 36 (2006) ] suffers from a strong background from the bulk medium. However, our results show quite the contrary, i.e., that our setup provides a very good signal contrast, due to an almost vanishing background level from the bulk solvent.

© 2007 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |

  1. I. Toytman, K. Cohn, T. Smith, D. Simanovskii, and D. Palanker, Opt. Lett. 32, 1941 (2007).
    [CrossRef] [PubMed]
  2. C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
    [CrossRef]
  3. C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
    [CrossRef]
  4. J.-X. Cheng and S. Xie, J. Phys. Chem. B 108, 827 (2004).
    [CrossRef]
  5. J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 105, 1277 (2001).
    [CrossRef]

2007

2006

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

2004

J.-X. Cheng and S. Xie, J. Phys. Chem. B 108, 827 (2004).
[CrossRef]

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

2001

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 105, 1277 (2001).
[CrossRef]

Appl. Phys. Lett.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, Appl. Phys. Lett. 84, 816 (2004).
[CrossRef]

J. Phys. Chem. B

J.-X. Cheng and S. Xie, J. Phys. Chem. B 108, 827 (2004).
[CrossRef]

J.-X. Cheng, A. Volkmer, L. D. Book, and X. S. Xie, J. Phys. Chem. B 105, 1277 (2001).
[CrossRef]

New J. Phys.

C. Heinrich, S. Bernet, and M. Ritsch-Marte, New J. Phys. 8, 36 (2006).
[CrossRef]

Opt. Lett.

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (1)

Fig. 1
Fig. 1

Examples of wide-field CARS images (no image processing): A, 3 μ m polystyrene beads in water, imaged at the 3050 cm 1 aromatic resonance ( λ Pump = 803.3 nm , λ Stokes = 1064 nm ), using 100 pulse pairs. The contrast between regions 1 and 2 is 24:1. B, Same image section as in A, but with the pump beam detuned to λ Pump = 800.4 nm . The squares at 1 and 3 have a contrast of 40:1. C, Dark-field image of a living alveolar type II cell. D, CARS image of the same cell ( 10 3 pulses). Vesicles with a diameter between 300 and 1000 nm generate a CARS signal at 2850 cm 1 ( λ Pump = 816.4 nm , λ Stokes = 1064 nm ). The contrast between the areas 4 and 5 is 5:1, the contrast between resonant and off-resonant ( λ Pump = 804 nm ) signals is 7:1.

Metrics