Abstract

Depth-resolved NADH autofluorescence images are shown to differentiate between normal and precancerous engineered tissues. An inverse power law behavior of the power spectral density (PSD) of these images is observed, indicating a self-affine organization of mitochondrial NADH at length scales 1–10μm. Power exponents of the PSD functions vary significantly with tissue depth and precancerous state, giving insight into the morphological changes associated with precancerous lesions and providing substantial potential for noninvasive clinical diagnosis of squamous epithelial lesions and tumors.

© 2007 Optical Society of America

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References

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2006 (1)

M. Hunter, V. Backman, G. Popescu, M. Kalashnikov, C. W. Boone, A. Wax, V. Gopal, K. Badizadegan, G. D. Stoner, and M. S. Feld, Phys. Rev. Lett. 97, 138102 (2006).
[CrossRef] [PubMed]

2002 (1)

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

2001 (1)

V. Backman, V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 7, 887 (2001).
[CrossRef]

2000 (1)

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, Nature 406, 35 (2000).
[CrossRef] [PubMed]

1998 (1)

A. J. Einstein, H.-S. Wu, and J. Gil, Phys. Rev. Lett. 80, 397 (1998).
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1997 (1)

C. Meyers, T. J. Mayer, and M. A. Ozbun, J. Virol. 71, 7381 (1997).
[PubMed]

1996 (2)

1995 (1)

K. E. Creek, G. Geslani, A. Batova, and L. Pirisi, Adv. Exp. Med. Biol. 375, 117 (1995).
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1988 (1)

D. J. McCance, R. Kopan, E. Fuchs, and L. A. Laimins, Proc. Natl. Acad. Sci. U.S.A. 85, 7169 (1988).
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1986 (2)

D. Asselineau, B. A. Bernard, C. Bailly, M. Darmon, and M. Pruniéras, J. Invest. Dermatol. 86, 181 (1986).
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[CrossRef]

Adv. Exp. Med. Biol. (1)

K. E. Creek, G. Geslani, A. Batova, and L. Pirisi, Adv. Exp. Med. Biol. 375, 117 (1995).
[PubMed]

Biophys. J. (1)

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, Biophys. J. 82, 2256 (2002).
[CrossRef] [PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

V. Backman, V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld, IEEE J. Sel. Top. Quantum Electron. 7, 887 (2001).
[CrossRef]

J. Invest. Dermatol. (1)

D. Asselineau, B. A. Bernard, C. Bailly, M. Darmon, and M. Pruniéras, J. Invest. Dermatol. 86, 181 (1986).
[CrossRef] [PubMed]

J. Virol. (1)

C. Meyers, T. J. Mayer, and M. A. Ozbun, J. Virol. 71, 7381 (1997).
[PubMed]

Nature (1)

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Muller, Q. Zhang, G. Zonios, E. Kline, T. McGillican, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, Nature 406, 35 (2000).
[CrossRef] [PubMed]

Opt. Commun. (1)

C. J. R. Sheppard, Opt. Commun. 122, 178 (1996).
[CrossRef]

Opt. Lett. (1)

Phys. Rev. Lett. (2)

A. J. Einstein, H.-S. Wu, and J. Gil, Phys. Rev. Lett. 80, 397 (1998).
[CrossRef]

M. Hunter, V. Backman, G. Popescu, M. Kalashnikov, C. W. Boone, A. Wax, V. Gopal, K. Badizadegan, G. D. Stoner, and M. S. Feld, Phys. Rev. Lett. 97, 138102 (2006).
[CrossRef] [PubMed]

Phys. Scr., T (1)

R. F. Voss, Phys. Scr., T 13, 27 (1986).
[CrossRef]

Proc. Natl. Acad. Sci. U.S.A. (1)

D. J. McCance, R. Kopan, E. Fuchs, and L. A. Laimins, Proc. Natl. Acad. Sci. U.S.A. 85, 7169 (1988).
[CrossRef] [PubMed]

Other (1)

B. B. Mandelbrot, The Fractal Geometry of Nature (W. H. Freeman, 2000).

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Figures (3)

Fig. 1
Fig. 1

PSD depth profiles for representative A, HFK and B, HPV rafts. C, The mean ± standard error of the variance of the PSD across the epithelial layer from three normal (green) and three precancerous (blue) engineered tissues.

Fig. 2
Fig. 2

NADH TPEF depth images from a single HFK raft at the superficial (A), intermediate (B), and basal layers (C), and corresponding PSD calculations with power law fits — see text for details.

Fig. 3
Fig. 3

NADH TPEF depth images from a single HPV raft at the A, superficial, B, intermediate, C, basal layers, and corresponding PSD calculations with power law fits — see text for details.

Tables (1)

Tables Icon

Table 1 PSD Power Exponents and Fractal Dimension of Normal and Precancerous Model Tissue TPEF Images, in High Spatial Frequency Domain ( 0.1 < κ < 1 μ m 1 )

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