Abstract

We investigated experimentally the evanescent field enhancement based on dielectric thin films in total internal reflection microscopy. The sample employed two layers of Al2O3 and SiO2 deposited on an SF10 glass substrate. Field intensity enhancement measured by fluorescent excitation of microbeads relative to that of a control sample without dielectric films was polarization dependent, determined as 4.2 and 2.4 for TE and TM polarizations, respectively, and was in good agreement with numerical results. The thin-film-based field enhancement was also applied to live-cell imaging of quantum dots, which confirmed the sensitivity enhancement qualitatively.

© 2007 Optical Society of America

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  1. H. Niwa, K. Yamamura, and J. Miyazaki, Gene 108, 193 (1991).
    [CrossRef] [PubMed]
  2. A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
    [CrossRef]
  3. M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
    [CrossRef] [PubMed]
  4. T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
    [CrossRef]
  5. Y. Levy, Nouv. Rev. Opt. Appl. 3, 25 (1972).
    [CrossRef]
  6. R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
    [CrossRef]
  7. P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
    [CrossRef]
  8. S. J. Yoon and D. Kim, J. Opt. Soc. Am. A 24, 2543 (2007).
    [CrossRef]
  9. E.D.Palik, ed., Handbook of Optical Constants of Solids (Academic, 1985).
  10. D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
    [CrossRef] [PubMed]
  11. Y. Sako, S. Minoguchi, and T. Yanagida, Nat. Cell Biol. 2, 168 (2000).
    [CrossRef] [PubMed]

2007 (1)

2004 (1)

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

2002 (1)

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

2000 (1)

Y. Sako, S. Minoguchi, and T. Yanagida, Nat. Cell Biol. 2, 168 (2000).
[CrossRef] [PubMed]

1997 (2)

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

1996 (1)

A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
[CrossRef]

1994 (1)

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

1991 (1)

H. Niwa, K. Yamamura, and J. Miyazaki, Gene 108, 193 (1991).
[CrossRef] [PubMed]

1985 (1)

E.D.Palik, ed., Handbook of Optical Constants of Solids (Academic, 1985).

1972 (1)

Y. Levy, Nouv. Rev. Opt. Appl. 3, 25 (1972).
[CrossRef]

Arndt-Jovin, D. J.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Aspect, A.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Crameri, A.

A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
[CrossRef]

Gan, X. S.

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

Grecco, H. E.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Gu, M.

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

Heintzmann, R.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Ibata, K.

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Ikawa, M.

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

Jares-Erijman, E. A.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Jovin, T. M.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Kaiser, R.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Ke, P. C.

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

Kim, D.

Kominami, K.

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

Kubota, M.

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Leipold, D.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Levy, Y.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Y. Levy, Nouv. Rev. Opt. Appl. 3, 25 (1972).
[CrossRef]

Lidke, D. S.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Mikoshiba, K.

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Minoguchi, S.

Y. Sako, S. Minoguchi, and T. Yanagida, Nat. Cell Biol. 2, 168 (2000).
[CrossRef] [PubMed]

Miyawaki, A.

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Miyazaki, J.

H. Niwa, K. Yamamura, and J. Miyazaki, Gene 108, 193 (1991).
[CrossRef] [PubMed]

Mlynek, J.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Nagai, T.

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Nagy, P.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Nakanishi, T.

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

Nishimune, Y.

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

Niwa, H.

H. Niwa, K. Yamamura, and J. Miyazaki, Gene 108, 193 (1991).
[CrossRef] [PubMed]

Okabe, M.

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

Park, E. S.

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Post, J. N.

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

Sako, Y.

Y. Sako, S. Minoguchi, and T. Yanagida, Nat. Cell Biol. 2, 168 (2000).
[CrossRef] [PubMed]

Schilders, S.

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

Seifert, W.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Stemmer, W. P. C.

A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
[CrossRef]

Szajman, J.

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

Tate, E.

A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
[CrossRef]

Vansteenkiste, N.

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Whitehorn, E. A.

A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
[CrossRef]

Yamamura, K.

H. Niwa, K. Yamamura, and J. Miyazaki, Gene 108, 193 (1991).
[CrossRef] [PubMed]

Yanagida, T.

Y. Sako, S. Minoguchi, and T. Yanagida, Nat. Cell Biol. 2, 168 (2000).
[CrossRef] [PubMed]

Yoon, S. J.

Bioimaging (1)

P. C. Ke, X. S. Gan, J. Szajman, S. Schilders, and M. Gu, Bioimaging 5, 1 (1997).
[CrossRef]

FEBS Lett. (1)

M. Okabe, M. Ikawa, K. Kominami, T. Nakanishi, and Y. Nishimune, FEBS Lett. 407, 313 (1997).
[CrossRef] [PubMed]

Gene (1)

H. Niwa, K. Yamamura, and J. Miyazaki, Gene 108, 193 (1991).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A (1)

Nat. Biotechnol. (2)

D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, Nat. Biotechnol. 22, 198 (2004).
[CrossRef] [PubMed]

T. Nagai, K. Ibata, E. S. Park, M. Kubota, K. Mikoshiba, and A. Miyawaki, Nat. Biotechnol. 20, 87 (2002).
[CrossRef]

Nat. Cell Biol. (1)

Y. Sako, S. Minoguchi, and T. Yanagida, Nat. Cell Biol. 2, 168 (2000).
[CrossRef] [PubMed]

Nature (1)

A. Crameri, E. A. Whitehorn, E. Tate, and W. P. C. Stemmer, Nature 14, 315 (1996).
[CrossRef]

Nouv. Rev. Opt. Appl. (1)

Y. Levy, Nouv. Rev. Opt. Appl. 3, 25 (1972).
[CrossRef]

Opt. Commun. (1)

R. Kaiser, Y. Levy, N. Vansteenkiste, A. Aspect, W. Seifert, D. Leipold, and J. Mlynek, Opt. Commun. 104, 234 (1994).
[CrossRef]

Other (1)

E.D.Palik, ed., Handbook of Optical Constants of Solids (Academic, 1985).

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Figures (3)

Fig. 1
Fig. 1

Calculated electric field intensity with and without thin film layers as a function of an incidence angle: (a) TE and (b) TM polarizations. The inset shows respective RIE.

Fig. 2
Fig. 2

Fluorescence images using microbeads with a 5 × objective: (a) TE and (b) TM polarizations. Images were taken over the boundary between the regions with and without thin-film layers. (c) Fluorescence intensity profile across the lines shown in (a) and (b).

Fig. 3
Fig. 3

(a) Bright-field and (b) TIRF images of A431 cells on a thin-film sample for TE polarization, compared with those on a reference sample without thin films, respectively in (c) and (d).

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