Abstract

Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein monolayers adsorbed on metal surfaces when surface plasmon resonance effects are utilized. The measured data, expressed in ellipsometric angles ψ and Δ are of several orders of magnitude larger in comparison with those from similar experiments performed with traditional ellipsometry. TIRE in spectral mode opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer microstructure.

© 2007 Optical Society of America

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References

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  1. M. Poksinski and H. Arwin, are preparing a paper to be called "Total internal reflection ellipsometry--Design of experimental setup."
  2. H. Arwin, M. Poksinski, and K. Johansen, Appl. Opt. 43, 3028 (2004).
    [CrossRef] [PubMed]
  3. R. M. A. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, 1987).
  4. H. G. Tompkins and E. A. Irene, eds., Handbook of Ellipsometry (Springer-Verlag, 2005).
    [CrossRef]
  5. S. Rekveld, Ellipsometric Studies of Protein Adsorption onto Hard Surfaces in a Flow Cell (Fedobruk, 1997).
  6. M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.
  7. P. Westphal and A. Bornmann, Sens. Actuators B 84, 278 (2002).
    [CrossRef]
  8. M. Poksinski and H. Arwin, Thin Solid Films 455-456, 716 (2004).
    [CrossRef]
  9. H. Arwin, in Handbook of Ellipsometry, H.G.Tompkins and E.A.Irene, eds. (Springer-Verlag, 2005), p. 799.
    [CrossRef]
  10. J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
    [CrossRef]

2004 (2)

2002 (1)

P. Westphal and A. Bornmann, Sens. Actuators B 84, 278 (2002).
[CrossRef]

1995 (1)

J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
[CrossRef]

Arwin, H.

H. Arwin, M. Poksinski, and K. Johansen, Appl. Opt. 43, 3028 (2004).
[CrossRef] [PubMed]

M. Poksinski and H. Arwin, Thin Solid Films 455-456, 716 (2004).
[CrossRef]

J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
[CrossRef]

M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.

M. Poksinski and H. Arwin, are preparing a paper to be called "Total internal reflection ellipsometry--Design of experimental setup."

H. Arwin, in Handbook of Ellipsometry, H.G.Tompkins and E.A.Irene, eds. (Springer-Verlag, 2005), p. 799.
[CrossRef]

Azzam, R. M. A.

R. M. A. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, 1987).

Bashara, N. M.

R. M. A. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, 1987).

Bornmann, A.

P. Westphal and A. Bornmann, Sens. Actuators B 84, 278 (2002).
[CrossRef]

Dzuho, H.

M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.

Irene, E. A.

H. G. Tompkins and E. A. Irene, eds., Handbook of Ellipsometry (Springer-Verlag, 2005).
[CrossRef]

Järrhed, J.-O.

M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.

Johansen, K.

Lundström, I.

J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
[CrossRef]

Mårtensson, J.

J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
[CrossRef]

Nygren, H.

J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
[CrossRef]

Poksinski, M.

M. Poksinski and H. Arwin, Thin Solid Films 455-456, 716 (2004).
[CrossRef]

H. Arwin, M. Poksinski, and K. Johansen, Appl. Opt. 43, 3028 (2004).
[CrossRef] [PubMed]

M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.

M. Poksinski and H. Arwin, are preparing a paper to be called "Total internal reflection ellipsometry--Design of experimental setup."

Rekveld, S.

S. Rekveld, Ellipsometric Studies of Protein Adsorption onto Hard Surfaces in a Flow Cell (Fedobruk, 1997).

Tompkins, H. G.

H. G. Tompkins and E. A. Irene, eds., Handbook of Ellipsometry (Springer-Verlag, 2005).
[CrossRef]

Westphal, P.

P. Westphal and A. Bornmann, Sens. Actuators B 84, 278 (2002).
[CrossRef]

Appl. Opt. (1)

J. Colloid Interface Sci. (1)

J. Mårtensson, H. Arwin, H. Nygren, and I. Lundström, J. Colloid Interface Sci. 174, 79 (1995).
[CrossRef]

Sens. Actuators B (1)

P. Westphal and A. Bornmann, Sens. Actuators B 84, 278 (2002).
[CrossRef]

Thin Solid Films (1)

M. Poksinski and H. Arwin, Thin Solid Films 455-456, 716 (2004).
[CrossRef]

Other (6)

H. Arwin, in Handbook of Ellipsometry, H.G.Tompkins and E.A.Irene, eds. (Springer-Verlag, 2005), p. 799.
[CrossRef]

M. Poksinski and H. Arwin, are preparing a paper to be called "Total internal reflection ellipsometry--Design of experimental setup."

R. M. A. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, 1987).

H. G. Tompkins and E. A. Irene, eds., Handbook of Ellipsometry (Springer-Verlag, 2005).
[CrossRef]

S. Rekveld, Ellipsometric Studies of Protein Adsorption onto Hard Surfaces in a Flow Cell (Fedobruk, 1997).

M. Poksinski, H. Dzuho, J.-O. Järrhed, and H. Arwin, in Eurosensors XIV, R.de Reus and S.Bouwstra, eds., (MIC, 2000), p. 239.

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Figures (7)

Fig. 1
Fig. 1

Schematic overview of the TIRE setup.

Fig. 2
Fig. 2

Kinetics after addition of HSA to a concentration of 1 mg ml recorded at a wavelength of 650 nm . HSA was added after 5 min .

Fig. 3
Fig. 3

Kinetics after addition of fibrinogen to a concentration of 1 mg ml recorded at a wavelength of 657 nm . Fibrinogen was added after 5 min .

Fig. 4
Fig. 4

Spectral changes due to adsorption of a monolayer of HSA.

Fig. 5
Fig. 5

Magnification of Fig. 4 for the range near the SPR resonance. Observe the difference in the spectra at 650 nm , where kinetics (Fig. 2) has been recorded.

Fig. 6
Fig. 6

Spectral changes due to the adsorption of a monolayer of fibrinogen.

Fig. 7
Fig. 7

Magnification of Fig. 6 for the range near the SPR resonance. Observe the difference in the spectra at 657 nm , where kinetics (Fig. 3) has been recorded.

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