Abstract

We have developed diffraction phase microscopy as a new technique for quantitative phase imaging of biological structures. The method combines the principles of common path interferometry and single-shot phase imaging and is characterized by subnanometer path-length stability and millisecond-scale acquisition time. The potential of the technique for quantifying nanoscale motions in live cells is demonstrated by experiments on red blood cells.

© 2006 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. D. J. Stephens and V. J. Allan, Science 300, 82 (2003).
    [CrossRef] [PubMed]
  2. A. Zilker, H. Engelhardt, and E. Sackmann, J. Phys. (Paris) 48, 2139 (1987).
    [CrossRef]
  3. R. Nowakowski, P. Luckham, and P. Winlove, Biochim. Biophys. Acta 1514, 170 (2001).
    [CrossRef] [PubMed]
  4. P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
    [PubMed]
  5. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, Opt. Lett. 29, 2503 (2004).
    [CrossRef] [PubMed]
  6. T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, Opt. Lett. 30, 1165 (2005).
    [CrossRef] [PubMed]
  7. G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. Lett. 10, 060503 (2005).
    [CrossRef]
  8. M. Hammer, D. Schweitzer, B. Michel, E. Thamm, and A. Kolb, Appl. Opt. 37, 7410 (1998).
    [CrossRef]
  9. F. Brochard and J. F. Lennon, J. Phys. (Paris) 36, 1035 (1975).
    [CrossRef]
  10. R. Lipowski and M. Girardet, Phys. Rev. Lett. 65, 2893 (1990).
    [CrossRef]
  11. N. Gov, A. G. Zilman, and S. Safran, Phys. Rev. Lett. 90, 228101 (2003).
    [CrossRef] [PubMed]
  12. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, Opt. Lett. 30, 468 (2005).
    [CrossRef] [PubMed]
  13. G.A.Dunn and D.Zicha, eds., Using DRIMAPS System of Transmission Interference Microscopy to Study Cell Behavior (Academic, 1997).
  14. M. A. Choma, A. K. Ellerbee, C. H. Yang, T. L. Creazzo, and J. A. Izatt, Opt. Lett. 30, 1162 (2005).
    [CrossRef] [PubMed]
  15. C. Joo, T. Akkin, B. Cense, B. H. Park, and J. E. de Boer, Opt. Lett. 30, 2131 (2005).
    [CrossRef] [PubMed]

2005

2004

2003

N. Gov, A. G. Zilman, and S. Safran, Phys. Rev. Lett. 90, 228101 (2003).
[CrossRef] [PubMed]

D. J. Stephens and V. J. Allan, Science 300, 82 (2003).
[CrossRef] [PubMed]

2001

R. Nowakowski, P. Luckham, and P. Winlove, Biochim. Biophys. Acta 1514, 170 (2001).
[CrossRef] [PubMed]

1998

1990

R. Lipowski and M. Girardet, Phys. Rev. Lett. 65, 2893 (1990).
[CrossRef]

1987

A. Zilker, H. Engelhardt, and E. Sackmann, J. Phys. (Paris) 48, 2139 (1987).
[CrossRef]

1975

F. Brochard and J. F. Lennon, J. Phys. (Paris) 36, 1035 (1975).
[CrossRef]

Akkin, T.

Allan, V. J.

D. J. Stephens and V. J. Allan, Science 300, 82 (2003).
[CrossRef] [PubMed]

Badizadegan, K.

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. Lett. 10, 060503 (2005).
[CrossRef]

G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, Opt. Lett. 29, 2503 (2004).
[CrossRef] [PubMed]

Best, C. A.

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. Lett. 10, 060503 (2005).
[CrossRef]

Brochard, F.

F. Brochard and J. F. Lennon, J. Phys. (Paris) 36, 1035 (1975).
[CrossRef]

Cense, B.

Choma, M. A.

Colomb, T.

Creazzo, T. L.

Cuche, E.

Dasari, R. R.

de Boer, J. E.

Deflores, L. P.

Depeursinge, C.

Ellerbee, A. K.

Emery, Y.

Engelhardt, H.

A. Zilker, H. Engelhardt, and E. Sackmann, J. Phys. (Paris) 48, 2139 (1987).
[CrossRef]

Feld, M. S.

Gambardella, L.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Girardet, M.

R. Lipowski and M. Girardet, Phys. Rev. Lett. 65, 2893 (1990).
[CrossRef]

Gov, N.

N. Gov, A. G. Zilman, and S. Safran, Phys. Rev. Lett. 90, 228101 (2003).
[CrossRef] [PubMed]

Hammer, M.

Ikeda, T.

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. Lett. 10, 060503 (2005).
[CrossRef]

T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, Opt. Lett. 30, 1165 (2005).
[CrossRef] [PubMed]

Iwai, H.

Izatt, J. A.

Joo, C.

Kolb, A.

Lennon, J. F.

F. Brochard and J. F. Lennon, J. Phys. (Paris) 36, 1035 (1975).
[CrossRef]

Lipowski, R.

R. Lipowski and M. Girardet, Phys. Rev. Lett. 65, 2893 (1990).
[CrossRef]

Luckham, P.

R. Nowakowski, P. Luckham, and P. Winlove, Biochim. Biophys. Acta 1514, 170 (2001).
[CrossRef] [PubMed]

Maccaglia, A.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Magistretti, P. J.

Malorni, W.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Marquet, P.

Matarrese, P.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Michel, B.

Minetti, M.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Nowakowski, R.

R. Nowakowski, P. Luckham, and P. Winlove, Biochim. Biophys. Acta 1514, 170 (2001).
[CrossRef] [PubMed]

Park, B. H.

Pietraforte, D.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Popescu, G.

Rappaz, B.

Sackmann, E.

A. Zilker, H. Engelhardt, and E. Sackmann, J. Phys. (Paris) 48, 2139 (1987).
[CrossRef]

Safran, S.

N. Gov, A. G. Zilman, and S. Safran, Phys. Rev. Lett. 90, 228101 (2003).
[CrossRef] [PubMed]

Schweitzer, D.

Stephens, D. J.

D. J. Stephens and V. J. Allan, Science 300, 82 (2003).
[CrossRef] [PubMed]

Straface, E.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Thamm, E.

Vaughan, J. C.

Vona, R.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

Winlove, P.

R. Nowakowski, P. Luckham, and P. Winlove, Biochim. Biophys. Acta 1514, 170 (2001).
[CrossRef] [PubMed]

Yang, C. H.

Zilker, A.

A. Zilker, H. Engelhardt, and E. Sackmann, J. Phys. (Paris) 48, 2139 (1987).
[CrossRef]

Zilman, A. G.

N. Gov, A. G. Zilman, and S. Safran, Phys. Rev. Lett. 90, 228101 (2003).
[CrossRef] [PubMed]

Appl. Opt.

Biochim. Biophys. Acta

R. Nowakowski, P. Luckham, and P. Winlove, Biochim. Biophys. Acta 1514, 170 (2001).
[CrossRef] [PubMed]

FASEB J.

P. Matarrese, E. Straface, D. Pietraforte, L. Gambardella, R. Vona, A. Maccaglia, M. Minetti, and W. Malorni, FASEB J. 19, 416 (2005).
[PubMed]

J. Biomed. Opt. Lett.

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, J. Biomed. Opt. Lett. 10, 060503 (2005).
[CrossRef]

J. Phys. (Paris)

A. Zilker, H. Engelhardt, and E. Sackmann, J. Phys. (Paris) 48, 2139 (1987).
[CrossRef]

F. Brochard and J. F. Lennon, J. Phys. (Paris) 36, 1035 (1975).
[CrossRef]

Opt. Lett.

Phys. Rev. Lett.

R. Lipowski and M. Girardet, Phys. Rev. Lett. 65, 2893 (1990).
[CrossRef]

N. Gov, A. G. Zilman, and S. Safran, Phys. Rev. Lett. 90, 228101 (2003).
[CrossRef] [PubMed]

Science

D. J. Stephens and V. J. Allan, Science 300, 82 (2003).
[CrossRef] [PubMed]

Other

G.A.Dunn and D.Zicha, eds., Using DRIMAPS System of Transmission Interference Microscopy to Study Cell Behavior (Academic, 1997).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (3)

Fig. 1
Fig. 1

Experimental setup. VPS, virtual source point; G, grating; IP, image plane; L 1 , 2 lenses ( f 1 , 2 , respective focal distances); SF, spatial filter (expanded in the inset).

Fig. 2
Fig. 2

a, Quantitative phase image of whole blood smear. The gradient bar represents the optical path length in nanometers. b, Temporal fluctuations of the spatial standard deviation associated with the no-sample field of view and of a single point ( P ) in the field of view, as indicated. The temporal standard deviations, σ, for these two signals are also shown.

Fig. 3
Fig. 3

Quantitative assessment of the single cell shape and dynamics. a, DPM image of a single RBC (the color bar represents the path length in nanometers). b, Thickness profile of the cell in a. c, Local thickness fluctuations at the three points on the cell indicated in a. The respective average thickness u and standard deviations σ are indicated.

Equations (2)

Equations on this page are rendered with MathJax. Learn more.

E ( x , y ) = E 0 e i [ ϕ 0 + β ( x + y ) ] + E 1 ( x , y ) e i ϕ ( x , y ) .
E 0 e i ϕ 0 1 A E 1 ( x , y ) e i ϕ ( x , y ) d x d y ,

Metrics