Abstract

We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue.

© 2004 Optical Society of America

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2003 (3)

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

M. Both, M. Vogel, R. H. A. Fink, and D. Uttenweiler, Proc. SPIE 5139, 112 (2003).
[CrossRef]

S.-W. Chu, T.-M. Liu, C.-K. Sun, C.-Y. Lin, and H. J. Tsai, Opt. Express 11, 933 (2003), http://www.opticsexpress.org .
[CrossRef] [PubMed]

2002 (4)

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

R. Littlefield and V. M. Fowler, Biophys. J. 82, 2548 (2002).
[CrossRef] [PubMed]

M.-P. Sauviat, A. Colas, and N. Pages, BMC Pharmacol. 2, 15 (2002).
[CrossRef]

R. E. Thompson, D. R. Larson, and W. W. Webb, Biophys. J. 82, 2775 (2002).
[CrossRef] [PubMed]

2000 (2)

1999 (1)

P. J. Campagnola, M.-de Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

1998 (1)

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

1997 (2)

Alfano, R. R.

Blanchard-Desce, M.

Both, M.

M. Both, M. Vogel, R. H. A. Fink, and D. Uttenweiler, Proc. SPIE 5139, 112 (2003).
[CrossRef]

Brandt, P. W.

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

Campagnola, P. J.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

P. J. Campagnola, M.-de Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Christie, R.

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

Chu, S.-W.

Colas, A.

M.-P. Sauviat, A. Colas, and N. Pages, BMC Pharmacol. 2, 15 (2002).
[CrossRef]

Colomo, F.

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

Fink, R. H. A.

M. Both, M. Vogel, R. H. A. Fink, and D. Uttenweiler, Proc. SPIE 5139, 112 (2003).
[CrossRef]

Fowler, V. M.

R. Littlefield and V. M. Fowler, Biophys. J. 82, 2548 (2002).
[CrossRef] [PubMed]

Guo, Y.

Harris, D.

Ho, P. P.

Hoppe, P. E.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Hyman, B. T.

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

Larson, D. R.

R. E. Thompson, D. R. Larson, and W. W. Webb, Biophys. J. 82, 2775 (2002).
[CrossRef] [PubMed]

Lewis, A.

P. J. Campagnola, M.-de Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Lin, C.-Y.

Littlefield, R.

R. Littlefield and V. M. Fowler, Biophys. J. 82, 2548 (2002).
[CrossRef] [PubMed]

Liu, F.

Liu, T.-M.

Loew, L. M.

P. J. Campagnola, M.-de Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Malone, C. J.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Mertz, J.

Millard, A. C.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Mohler, W. A.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Moreaux, L.

Narahashi, T.

T. Narahashi, J. Pharmacol. Exp. Ther. 294, 1 (2000).
[PubMed]

Nikitin, A. Yu

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

Pages, N.

M.-P. Sauviat, A. Colas, and N. Pages, BMC Pharmacol. 2, 15 (2002).
[CrossRef]

Piroddi, N.

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

Poggesi, C.

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

Sacks, P.

Sandre, O.

Sauviat, M.-P.

M.-P. Sauviat, A. Colas, and N. Pages, BMC Pharmacol. 2, 15 (2002).
[CrossRef]

Savage, H.

Schantz, S.

Squire, J. M.

J. M. Squire, Curr. Opin. Struct. Biol. 7, 247 (1997).
[CrossRef] [PubMed]

Sun, C.-K.

Terasaki, M.

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

Tesi, C.

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

Thompson, R. E.

R. E. Thompson, D. R. Larson, and W. W. Webb, Biophys. J. 82, 2775 (2002).
[CrossRef] [PubMed]

Tsai, H. J.

Uttenweiler, D.

M. Both, M. Vogel, R. H. A. Fink, and D. Uttenweiler, Proc. SPIE 5139, 112 (2003).
[CrossRef]

Vogel, M.

M. Both, M. Vogel, R. H. A. Fink, and D. Uttenweiler, Proc. SPIE 5139, 112 (2003).
[CrossRef]

Webb, W. W.

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

R. E. Thompson, D. R. Larson, and W. W. Webb, Biophys. J. 82, 2775 (2002).
[CrossRef] [PubMed]

Wei, M.-de

P. J. Campagnola, M.-de Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

Williams, R. M.

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

Zhadin, N.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

Biophys. J. (5)

P. J. Campagnola, M.-de Wei, A. Lewis, and L. M. Loew, Biophys. J. 77, 3341 (1999).
[CrossRef] [PubMed]

R. Littlefield and V. M. Fowler, Biophys. J. 82, 2548 (2002).
[CrossRef] [PubMed]

R. E. Thompson, D. R. Larson, and W. W. Webb, Biophys. J. 82, 2775 (2002).
[CrossRef] [PubMed]

P. W. Brandt, F. Colomo, N. Piroddi, C. Poggesi, and C. Tesi, Biophys. J. 74, 1994 (1998).
[CrossRef] [PubMed]

P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, Biophys. J. 82, 493 (2002).
[CrossRef]

BMC Pharmacol. (1)

M.-P. Sauviat, A. Colas, and N. Pages, BMC Pharmacol. 2, 15 (2002).
[CrossRef]

Curr. Opin. Struct. Biol. (1)

J. M. Squire, Curr. Opin. Struct. Biol. 7, 247 (1997).
[CrossRef] [PubMed]

J. Pharmacol. Exp. Ther. (1)

T. Narahashi, J. Pharmacol. Exp. Ther. 294, 1 (2000).
[PubMed]

Opt. Express (1)

Opt. Lett. (2)

Proc. Natl. Acad. Sci. USA (1)

W. R. Zipfel, R. M. Williams, R. Christie, A. Yu Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003).
[CrossRef]

Proc. SPIE (1)

M. Both, M. Vogel, R. H. A. Fink, and D. Uttenweiler, Proc. SPIE 5139, 112 (2003).
[CrossRef]

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