Abstract

We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.

© 1997 of America

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  1. T. Wilson and C. J.R. Sheppard, in Theory and Practice of Scanning Optical Microscopy, Academic, London, 1984
  2. , ed., in Confocal Microscopy, Academic, London, 1990
  3. B. Neumann, in Proc. SPIE, 1989
  4. C. G. Morgan, January 10, 1995, U.S. patent 5,381,236
  5. A. B. Carlson, in Communication Systems, McGraw-Hill, New York, 1988
  6. P. A. Stokseth, J. Opt. Soc. Am., 59, 1314, 1969
  7. T. Wilson and R. Juskaitis, Bioimaging, 3, 35, 1995

1995

T. Wilson and R. Juskaitis, Bioimaging, 3, 35, 1995

1990

, ed., in Confocal Microscopy, Academic, London, 1990

1988

A. B. Carlson, in Communication Systems, McGraw-Hill, New York, 1988

1984

T. Wilson and C. J.R. Sheppard, in Theory and Practice of Scanning Optical Microscopy, Academic, London, 1984

1969

Carlson, A. B.

A. B. Carlson, in Communication Systems, McGraw-Hill, New York, 1988

Juskaitis, R.

T. Wilson and R. Juskaitis, Bioimaging, 3, 35, 1995

Morgan, C. G.

C. G. Morgan, January 10, 1995, U.S. patent 5,381,236

Neumann, B.

B. Neumann, in Proc. SPIE, 1989

Sheppard, C. J.R.

T. Wilson and C. J.R. Sheppard, in Theory and Practice of Scanning Optical Microscopy, Academic, London, 1984

Stokseth, P. A.

Wilson, T.

T. Wilson and R. Juskaitis, Bioimaging, 3, 35, 1995

T. Wilson and C. J.R. Sheppard, in Theory and Practice of Scanning Optical Microscopy, Academic, London, 1984

Bioimaging

T. Wilson and R. Juskaitis, Bioimaging, 3, 35, 1995

J. Opt. Soc. Am.

Other

T. Wilson and C. J.R. Sheppard, in Theory and Practice of Scanning Optical Microscopy, Academic, London, 1984

, ed., in Confocal Microscopy, Academic, London, 1990

B. Neumann, in Proc. SPIE, 1989

C. G. Morgan, January 10, 1995, U.S. patent 5,381,236

A. B. Carlson, in Communication Systems, McGraw-Hill, New York, 1988

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