Abstract

We present measurements of biomolecular binding reactions, using a new type of integrated-optical biosensor based on a planar directional coupler structure. The device is fabricated by Ag+ –Na+ ion exchange in glass, and definition of the sensing region is achieved by use of transparent fluoropolymer isolation layers formed by thermal evaporation. The suitability of the sensor for application to the detection of environmental pollutants is considered.

© 1996 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. A. M. Hutchinson, Mol. Biotechnol. 3, 47 (1995).
    [CrossRef] [PubMed]
  2. P. V. Lambeck, Sensors Actuators B 8, 103 (1992).
    [CrossRef]
  3. R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.
  4. G. Gauglitz, J. Ingenhoff, Z. Fresenius, J. Anal., Chem. 349, 355 (1994).
  5. R. G. Heideman, R. P. H. Kooyman, J. Greve, Sensors Actuators B 10, 209 (1993).
    [CrossRef]
  6. A. Yariv, Optical Electronics, 4th ed.(Saunders, Philadelphia, Pa., 1991).
  7. J. H. Lowry, J. S. Mendlowitz, N. S. Subramanian, Opt. Eng. 31, 1982 (1992).
    [CrossRef]
  8. Dipartimento di Elettronica, Politecnico di Torino, Turin, Italy.
  9. B. J. Luff, R. D. Harris, J. S. Wilkinson, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.
  10. T. C. Nason, J. A. Moore, T.-M. Lu, Appl. Phys. Lett. 60, 1866 (1992).
    [CrossRef]
  11. J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).
  12. A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
    [CrossRef]

1995 (2)

A. M. Hutchinson, Mol. Biotechnol. 3, 47 (1995).
[CrossRef] [PubMed]

A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
[CrossRef]

1994 (1)

G. Gauglitz, J. Ingenhoff, Z. Fresenius, J. Anal., Chem. 349, 355 (1994).

1993 (2)

R. G. Heideman, R. P. H. Kooyman, J. Greve, Sensors Actuators B 10, 209 (1993).
[CrossRef]

J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).

1992 (3)

P. V. Lambeck, Sensors Actuators B 8, 103 (1992).
[CrossRef]

J. H. Lowry, J. S. Mendlowitz, N. S. Subramanian, Opt. Eng. 31, 1982 (1992).
[CrossRef]

T. C. Nason, J. A. Moore, T.-M. Lu, Appl. Phys. Lett. 60, 1866 (1992).
[CrossRef]

Brecht, A.

A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
[CrossRef]

Drapp, B.

J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).

Fresenius, Z.

G. Gauglitz, J. Ingenhoff, Z. Fresenius, J. Anal., Chem. 349, 355 (1994).

J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).

Gauglitz, G.

A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
[CrossRef]

G. Gauglitz, J. Ingenhoff, Z. Fresenius, J. Anal., Chem. 349, 355 (1994).

J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).

Greve, J.

R. G. Heideman, R. P. H. Kooyman, J. Greve, Sensors Actuators B 10, 209 (1993).
[CrossRef]

Harris, R. D.

B. J. Luff, R. D. Harris, J. S. Wilkinson, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Heideman, R. G.

R. G. Heideman, R. P. H. Kooyman, J. Greve, Sensors Actuators B 10, 209 (1993).
[CrossRef]

Hutchinson, A. M.

A. M. Hutchinson, Mol. Biotechnol. 3, 47 (1995).
[CrossRef] [PubMed]

Ingenhoff, J.

G. Gauglitz, J. Ingenhoff, Z. Fresenius, J. Anal., Chem. 349, 355 (1994).

J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).

Kooyman, R. P. H.

R. G. Heideman, R. P. H. Kooyman, J. Greve, Sensors Actuators B 10, 209 (1993).
[CrossRef]

Lambeck, P. V.

P. V. Lambeck, Sensors Actuators B 8, 103 (1992).
[CrossRef]

Lang, G.

A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
[CrossRef]

Lowry, J. H.

J. H. Lowry, J. S. Mendlowitz, N. S. Subramanian, Opt. Eng. 31, 1982 (1992).
[CrossRef]

Lu, T.-M.

T. C. Nason, J. A. Moore, T.-M. Lu, Appl. Phys. Lett. 60, 1866 (1992).
[CrossRef]

Luff, B. J.

R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

B. J. Luff, R. D. Harris, J. S. Wilkinson, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Mendlowitz, J. S.

J. H. Lowry, J. S. Mendlowitz, N. S. Subramanian, Opt. Eng. 31, 1982 (1992).
[CrossRef]

Moore, J. A.

T. C. Nason, J. A. Moore, T.-M. Lu, Appl. Phys. Lett. 60, 1866 (1992).
[CrossRef]

Nason, T. C.

T. C. Nason, J. A. Moore, T.-M. Lu, Appl. Phys. Lett. 60, 1866 (1992).
[CrossRef]

Piehler, J.

A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
[CrossRef]

Schiffrin, D. J.

R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Subramanian, N. S.

J. H. Lowry, J. S. Mendlowitz, N. S. Subramanian, Opt. Eng. 31, 1982 (1992).
[CrossRef]

Wilkinson, J. S.

B. J. Luff, R. D. Harris, J. S. Wilkinson, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Wilson, R.

R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Yariv, A.

A. Yariv, Optical Electronics, 4th ed.(Saunders, Philadelphia, Pa., 1991).

Anal. Chim. Acta (1)

A. Brecht, J. Piehler, G. Lang, G. Gauglitz, Anal. Chim. Acta 311, 289 (1995).
[CrossRef]

Appl. Phys. Lett. (1)

T. C. Nason, J. A. Moore, T.-M. Lu, Appl. Phys. Lett. 60, 1866 (1992).
[CrossRef]

J. Anal, . Chem. (1)

J. Ingenhoff, B. Drapp, G. Gauglitz, Z. Fresenius, J. Anal, . Chem. 346, 580 (1993).

J. Anal., Chem. (1)

G. Gauglitz, J. Ingenhoff, Z. Fresenius, J. Anal., Chem. 349, 355 (1994).

Mol. Biotechnol. (1)

A. M. Hutchinson, Mol. Biotechnol. 3, 47 (1995).
[CrossRef] [PubMed]

Opt. Eng. (1)

J. H. Lowry, J. S. Mendlowitz, N. S. Subramanian, Opt. Eng. 31, 1982 (1992).
[CrossRef]

Sensors Actuators B (2)

P. V. Lambeck, Sensors Actuators B 8, 103 (1992).
[CrossRef]

R. G. Heideman, R. P. H. Kooyman, J. Greve, Sensors Actuators B 10, 209 (1993).
[CrossRef]

Other (4)

A. Yariv, Optical Electronics, 4th ed.(Saunders, Philadelphia, Pa., 1991).

R. D. Harris, B. J. Luff, J. S. Wilkinson, R. Wilson, D. J. Schiffrin, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Dipartimento di Elettronica, Politecnico di Torino, Turin, Italy.

B. J. Luff, R. D. Harris, J. S. Wilkinson, presented at the Seventh European Conference on Integrated Optics, Delft, The Netherlands, April 3–6, 1995.

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (5)

Fig. 1
Fig. 1

Directional coupler sensor configuration.

Fig. 2
Fig. 2

Measured variation of coupler outputs with the superstrate index.

Fig. 3
Fig. 3

Illustration of the buildup of biotinylated BSA– polystreptavidin biolayers.

Fig. 4
Fig. 4

(a) Change in coupler outputs P1 and P2s on addition of the tenth layer (polystreptavidin) and the eleventh layer (BSA); (b) sum and difference of the outputs shown in (a).

Fig. 5
Fig. 5

Response of the sensor to the addition of multiple biolayers.

Equations (1)

Equations on this page are rendered with MathJax. Learn more.

P 2 = sin 2 { [ ( κ L ) 2 + ( Δ β L / 2 ) 2 ] } 1 / 2 1 + ( Δ β / 2 κ ) 2 P 0 ,

Metrics