Abstract

Resolution in potential three-photon fluorescence scanning microscopy is discussed in terms of the three-dimensional optical transfer function. Images of layers and sharp edges are presented for a comparison of the resolution with that in two-photon fluorescence microscopic imaging. For the same fluorescence wavelength the resolution is almost the same in both cases. However, for a given illumination wavelength the resolution for imaging a thick object in the case of three-photon fluorescence imaging can be improved by as much as 40–50% relative to that in two-photon imaging.

© 1996 Optical Society of America

Resolution in three-photon fluorescence scanning microscopy: errata

Min Gu
Opt. Lett. 21(17) 1414-1414 (1996)

Three-dimensional optical-transfer-function analysis of fiber-optical two-photon fluorescence microscopy

Min Gu and Damian Bird
J. Opt. Soc. Am. A 20(5) 941-947 (2003)

Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation

J. T. Frohn, H. F. Knapp, and A. Stemmer
Opt. Lett. 26(11) 828-830 (2001)

Optical transfer functions for confocal theta fluorescence microscopy

Steffen Lindek and Ernst H. K. Stelzer
J. Opt. Soc. Am. A 13(3) 479-482 (1996)

Image formation in image scanning microscopy, including the case of two-photon excitation

Colin J. R. Sheppard, Marco Castello, Giorgio Tortarolo, Giuseppe Vicidomini, and Alberto Diaspro
J. Opt. Soc. Am. A 34(8) 1339-1350 (2017)