Abstract

Light-sheet fluorescence microscopy (LSFM) helps investigate small structures in developing cells and tissue for three-dimensional localization microscopy and large-field brain imaging in neuroscience. Lattice light-sheet microscopy is a recent development with great potential to improve axial resolution and usable field sizes, thus improving imaging speed. In contrast to the commonly employed Gaussian beams for light-sheet generation in conventional LSFM, in lattice light-sheet microscopy an array of low diverging Bessel beams with a suppressed side lobe structure is used. We developed a facile elementary lattice light-sheet microscope using a micro-fabricated fixed ring mask for lattice light-sheet generation. In our setup, optical hardware elements enable a stable and simple illumination path without the need for spatial light modulators. This setup, in combination with long-working distance objectives and the possibility for simultaneous dual-color imaging, provides optimal conditions for imaging extended optically cleared tissue samples. We here present experimental data of fluorescently stained neurons and neurites from mouse hippocampus following tissue expansion and demonstrate the high homogeneous resolution throughout the entire imaged volume. Utilizing our purpose-built lattice light-sheet microscope, we reached a homogeneous excitation and an axial resolution of 1.2 µm over a field of view of (333 µm)2.

© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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  1. R. M. Power and J. Huisken, “A guide to light-sheet fluorescence microscopy for multiscale imaging,” Nat. Methods 14(4), 360–373 (2017).
    [Crossref]
  2. A.-K. Gustavsson, P. N. Petrov, and W. E. Moerner, “Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited],” Opt. Express 26(10), 13122–13147 (2018).
    [Crossref]
  3. S. Corsetti, F. Gunn-Moore, and K. Dholakia, “Light sheet fluorescence microscopy for neuroscience,” J. Neurosci. Methods 319, 16–27 (2019).
    [Crossref]
  4. E. Baumgart and U. Kubitscheck, “Scanned light sheet microscopy with confocal slit detection,” Opt. Express 20(19), 21805–21814 (2012).
    [Crossref]
  5. L. Silvestri, A. Bria, L. Sacconi, G. Iannello, and F. S. Pavone, “Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain,” Opt. Express 20(18), 20582–20598 (2012).
    [Crossref]
  6. P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
    [Crossref]
  7. T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8(9), 757–760 (2011).
    [Crossref]
  8. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
    [Crossref]
  9. F. O. Fahrbach and A. Rohrbach, “Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media,” Nat. Commun. 3(1), 632 (2012).
    [Crossref]
  10. T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
    [Crossref]
  11. M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
    [Crossref]
  12. Y. Wan, K. McDole, and P. J. Keller, “Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes,” Annu. Rev. Cell Dev. Biol. 35(1), 655–681 (2019).
    [Crossref]
  13. B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
    [Crossref]
  14. J. Bürgers, I. Pavlova, J. E. Rodriguez-Gatica, C. Henneberger, M. Oeller, J. A. Ruland, J. P. Siebrasse, U. Kubitscheck, and M. K. Schwarz, “Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution,” Neurophotonics 6(01), 1 (2019).
    [Crossref]
  15. K. L. Ellefsen and I. Parker, “Dynamic Ca2+ imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca2+ puffs,” Cell Calcium 71, 34–44 (2018).
    [Crossref]
  16. J. G. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16(10), 7142–7152 (2008).
    [Crossref]
  17. A. Jain, A. H. J. Yang, and D. Erickson, “Gel-based optical waveguides with live cell encapsulation and integrated microfluidics,” Opt. Lett. 37(9), 1472–1474 (2012).
    [Crossref]
  18. J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
    [Crossref]
  19. F. Chen, P. W. Tillberg, and E. S. Boyden, “Optical imaging. Expansion microscopy,” Science 347(6221), 543–548 (2015).
    [Crossref]
  20. T. J. Chozinski, A. R. Halpern, H. Okawa, H.-J. Kim, G. J. Tremel, R. O. L. Wong, and J. C. Vaughan, “Expansion microscopy with conventional antibodies and fluorescent proteins,” Nat. Methods 13(6), 485–488 (2016).
    [Crossref]

2019 (3)

S. Corsetti, F. Gunn-Moore, and K. Dholakia, “Light sheet fluorescence microscopy for neuroscience,” J. Neurosci. Methods 319, 16–27 (2019).
[Crossref]

Y. Wan, K. McDole, and P. J. Keller, “Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes,” Annu. Rev. Cell Dev. Biol. 35(1), 655–681 (2019).
[Crossref]

J. Bürgers, I. Pavlova, J. E. Rodriguez-Gatica, C. Henneberger, M. Oeller, J. A. Ruland, J. P. Siebrasse, U. Kubitscheck, and M. K. Schwarz, “Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution,” Neurophotonics 6(01), 1 (2019).
[Crossref]

2018 (2)

K. L. Ellefsen and I. Parker, “Dynamic Ca2+ imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca2+ puffs,” Cell Calcium 71, 34–44 (2018).
[Crossref]

A.-K. Gustavsson, P. N. Petrov, and W. E. Moerner, “Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited],” Opt. Express 26(10), 13122–13147 (2018).
[Crossref]

2017 (1)

R. M. Power and J. Huisken, “A guide to light-sheet fluorescence microscopy for multiscale imaging,” Nat. Methods 14(4), 360–373 (2017).
[Crossref]

2016 (1)

T. J. Chozinski, A. R. Halpern, H. Okawa, H.-J. Kim, G. J. Tremel, R. O. L. Wong, and J. C. Vaughan, “Expansion microscopy with conventional antibodies and fluorescent proteins,” Nat. Methods 13(6), 485–488 (2016).
[Crossref]

2015 (1)

F. Chen, P. W. Tillberg, and E. S. Boyden, “Optical imaging. Expansion microscopy,” Science 347(6221), 543–548 (2015).
[Crossref]

2014 (2)

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

2012 (5)

A. Jain, A. H. J. Yang, and D. Erickson, “Gel-based optical waveguides with live cell encapsulation and integrated microfluidics,” Opt. Lett. 37(9), 1472–1474 (2012).
[Crossref]

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

E. Baumgart and U. Kubitscheck, “Scanned light sheet microscopy with confocal slit detection,” Opt. Express 20(19), 21805–21814 (2012).
[Crossref]

L. Silvestri, A. Bria, L. Sacconi, G. Iannello, and F. S. Pavone, “Confocal light sheet microscopy: micron-scale neuroanatomy of the entire mouse brain,” Opt. Express 20(18), 20582–20598 (2012).
[Crossref]

F. O. Fahrbach and A. Rohrbach, “Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media,” Nat. Commun. 3(1), 632 (2012).
[Crossref]

2011 (3)

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8(9), 757–760 (2011).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

2008 (2)

J. G. Ritter, R. Veith, J. P. Siebrasse, and U. Kubitscheck, “High-contrast single-particle tracking by selective focal plane illumination microscopy,” Opt. Express 16(10), 7142–7152 (2008).
[Crossref]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref]

Arganda-Carreras, I.

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

Baumgart, E.

Bembenek, J. N.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Betzig, E.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Böhme, R.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Boyden, E. S.

F. Chen, P. W. Tillberg, and E. S. Boyden, “Optical imaging. Expansion microscopy,” Science 347(6221), 543–548 (2015).
[Crossref]

Bria, A.

Bürgers, J.

J. Bürgers, I. Pavlova, J. E. Rodriguez-Gatica, C. Henneberger, M. Oeller, J. A. Ruland, J. P. Siebrasse, U. Kubitscheck, and M. K. Schwarz, “Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution,” Neurophotonics 6(01), 1 (2019).
[Crossref]

Cardona, A.

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

Chen, B.-C.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Chen, F.

F. Chen, P. W. Tillberg, and E. S. Boyden, “Optical imaging. Expansion microscopy,” Science 347(6221), 543–548 (2015).
[Crossref]

Choi, J. M.

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8(9), 757–760 (2011).
[Crossref]

Chozinski, T. J.

T. J. Chozinski, A. R. Halpern, H. Okawa, H.-J. Kim, G. J. Tremel, R. O. L. Wong, and J. C. Vaughan, “Expansion microscopy with conventional antibodies and fluorescent proteins,” Nat. Methods 13(6), 485–488 (2016).
[Crossref]

Cižmár, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Coll-Lladó, C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Corsetti, S.

S. Corsetti, F. Gunn-Moore, and K. Dholakia, “Light sheet fluorescence microscopy for neuroscience,” J. Neurosci. Methods 319, 16–27 (2019).
[Crossref]

Dalgarno, H. I. C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Davidson, M. W.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Dholakia, K.

S. Corsetti, F. Gunn-Moore, and K. Dholakia, “Light sheet fluorescence microscopy for neuroscience,” J. Neurosci. Methods 319, 16–27 (2019).
[Crossref]

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Eliceiri, K.

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

Ellefsen, K. L.

K. L. Ellefsen and I. Parker, “Dynamic Ca2+ imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca2+ puffs,” Cell Calcium 71, 34–44 (2018).
[Crossref]

English, B. P.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Erickson, D.

Ermolayev, V.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Fahrbach, F. O.

F. O. Fahrbach and A. Rohrbach, “Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media,” Nat. Commun. 3(1), 632 (2012).
[Crossref]

Ferrier, D. E. K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Fraser, S. E.

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8(9), 757–760 (2011).
[Crossref]

Friedrich, M.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Frise, E.

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

Fritz-Laylin, L.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Galbraith, C. G.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Galbraith, J. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Gan, Q.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Gao, L.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

Grill, S. W.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Gunn-Moore, F.

S. Corsetti, F. Gunn-Moore, and K. Dholakia, “Light sheet fluorescence microscopy for neuroscience,” J. Neurosci. Methods 319, 16–27 (2019).
[Crossref]

Gunn-Moore, F. J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Gustavsson, A.-K.

Halpern, A. R.

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B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
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Veith, R.

Vettenburg, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

Wan, Y.

Y. Wan, K. McDole, and P. J. Keller, “Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes,” Annu. Rev. Cell Dev. Biol. 35(1), 655–681 (2019).
[Crossref]

Wang, J. T.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Wang, K.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

White, D. J.

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref]

Wong, R. O. L.

T. J. Chozinski, A. R. Halpern, H. Okawa, H.-J. Kim, G. J. Tremel, R. O. L. Wong, and J. C. Vaughan, “Expansion microscopy with conventional antibodies and fluorescent proteins,” Nat. Methods 13(6), 485–488 (2016).
[Crossref]

Wu, X. S.

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

Yang, A. H. J.

Annu. Rev. Cell Dev. Biol. (1)

Y. Wan, K. McDole, and P. J. Keller, “Light-Sheet Microscopy and Its Potential for Understanding Developmental Processes,” Annu. Rev. Cell Dev. Biol. 35(1), 655–681 (2019).
[Crossref]

Biophys. J. (1)

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref]

Cell Calcium (1)

K. L. Ellefsen and I. Parker, “Dynamic Ca2+ imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca2+ puffs,” Cell Calcium 71, 34–44 (2018).
[Crossref]

J. Neurosci. Methods (1)

S. Corsetti, F. Gunn-Moore, and K. Dholakia, “Light sheet fluorescence microscopy for neuroscience,” J. Neurosci. Methods 319, 16–27 (2019).
[Crossref]

Nat. Commun. (1)

F. O. Fahrbach and A. Rohrbach, “Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media,” Nat. Commun. 3(1), 632 (2012).
[Crossref]

Nat. Methods (6)

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref]

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8(9), 757–760 (2011).
[Crossref]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref]

J. Schindelin, I. Arganda-Carreras, E. Frise, V. Kaynig, M. Longair, T. Pietzsch, S. Preibisch, C. Rueden, S. Saalfeld, B. Schmid, J.-Y. Tinevez, D. J. White, V. Hartenstein, K. Eliceiri, P. Tomancak, and A. Cardona, “Fiji: an open-source platform for biological-image analysis,” Nat. Methods 9(7), 676–682 (2012).
[Crossref]

R. M. Power and J. Huisken, “A guide to light-sheet fluorescence microscopy for multiscale imaging,” Nat. Methods 14(4), 360–373 (2017).
[Crossref]

T. J. Chozinski, A. R. Halpern, H. Okawa, H.-J. Kim, G. J. Tremel, R. O. L. Wong, and J. C. Vaughan, “Expansion microscopy with conventional antibodies and fluorescent proteins,” Nat. Methods 13(6), 485–488 (2016).
[Crossref]

Neurophotonics (1)

J. Bürgers, I. Pavlova, J. E. Rodriguez-Gatica, C. Henneberger, M. Oeller, J. A. Ruland, J. P. Siebrasse, U. Kubitscheck, and M. K. Schwarz, “Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution,” Neurophotonics 6(01), 1 (2019).
[Crossref]

Opt. Express (4)

Opt. Lett. (1)

Science (3)

F. Chen, P. W. Tillberg, and E. S. Boyden, “Optical imaging. Expansion microscopy,” Science 347(6221), 543–548 (2015).
[Crossref]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref]

B.-C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A.-C. Reymann, R. Böhme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution,” Science 346(6208), 1257998 (2014).
[Crossref]

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Figures (9)

Fig. 1.
Fig. 1. Mask with three slits. (a) Definition of mask parameters. (b) Sketch of the mask with D1=7.5 mm, d=0.8 mm, width T=100 µm. (c) Simulated intensity distribution in the central maximum of the diffraction pattern.
Fig. 2.
Fig. 2. Setup of the lattice light-sheet microscope. (a) Top view of the illumination beam path comprising the mask (upper section and left) and the Gaussian illumination (right-hand side). Conjugate planes were marked in red. (b) Side view of the instrument showing the detection beam path.
Fig. 3.
Fig. 3. Optical lattice produced in the sample chamber. (a) Image of the lattice over a field of (333 µm)2. (b) Theoretically calculated intensity distribution in y-direction. (c) Intensity profile in y-direction from Fig. 3(a).
Fig. 4.
Fig. 4. Optical lattices produced in the sample chamber using different excitation wavelengths over a field of (100 µm)2 for an excitation wavelength of (a) 488 nm, (b) 561 nm, and (c) 638 nm.
Fig. 5.
Fig. 5. Images of the various illumination fields imaged in fluorescein: (a) Narrow Gaussian beam, (b) wide Gaussian beam. The narrow beam resulted from a two-fold expansion of the wide Gaussian beam before the illumination objective, what results in a thinner beam waist in the sample plane. (c) Intensity profiles in illumination direction for the two Gaussian beams and the lattice illumination. (red, lattice; green, wide Gaussian beam; blue, narrow Gaussian beam)
Fig. 6.
Fig. 6. Axial resolution. (a) Determination of FWHM values of an exemplary bead (blue dotted line, normalized intensity values along the detection axis; green dotted line, normalized intensity values in lateral direction; full line, results of fitting a Gaussian function; FWHM indicated by the black line). (b) Axial resolution as a function of position along the illumination direction. The colored curves show the mean values of N=10 beads in each x-interval, the shaded regions indicate the standard deviations (red, lattice; green, wide Gaussian beam; blue, narrow Gaussian beam)
Fig. 7.
Fig. 7. Antibody stained expanded mouse brain section imaged with the lattice light-sheet. (a) Large stitched volume showing a neuron inside the mouse hippocampus. (b) The close-up shows a rendered dendrite where fine details are resolved. (d) Close up of the dendritic spines marked by the red box in (c). (e) Intensity profile of the spine neck marked with the red line in (e). The black line marks the FWHM with a length of 640 nm. All scales refer to expanded samples.
Fig. 8.
Fig. 8. Two-color imaging of a set of granule cells in an expanded mouse brain hippocampus section. (a) Large volume showing immunostained EGFP-expressing granule cells and dendrites (green) as well as cell nuclei stained by DAPI (pink) excited with 488 and 405 nm, respectively. (b) A single frame of the stack showing the overlay of the cell nucleus and the soma.
Fig. 9.
Fig. 9. Maximum intensity projections of dendrites in an expanded mouse hippocampus. (a, c, e): Lattice, (b, d, f): Gaussian. The divergence of the Gaussian beam generates blurred areas at image edges (red box). In the image center the Gaussian beam illumination reaches the resolution of the lattice (blue box).

Tables (1)

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Table 1. Comparison of beam waists, field of view and measured axial resolution.

Equations (1)

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L R = n π ω 0 2 λ ,

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