Abstract

Lensless quantitative phase imaging is of high interest for obtaining a large field of view (FOV), typically the size of the camera chip, to observe biological cell material with high contrast. It has the potential to be widely spread due to its inherent simplicity. However, the tradeoff is the added complexity due to the illumination. Current illumination systems are several centimeters away from the sample, use mechanics to obtain super resolution (i.e., smaller than the detector pixel size) or different illumination directions, and block the view to the sample. In this paper, we propose and demonstrate a side illumination system which reduces the height by an order of magnitude while providing an unobstructed view of the sample. We achieve this by shaping the illumination using multiplexed analog holograms that produce 9 illumination angles. We demonstrate experimentally imaging of phase samples with a FOV of ~17mm2.

© 2017 Optical Society of America

Full Article  |  PDF Article
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References

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2016 (2)

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

F. Soulez, É. Thiébaut, A. Schutz, A. Ferrari, F. Courbin, and M. Unser, “Proximity operators for phase retrieval,” Appl. Opt. 55(26), 7412–7421 (2016).
[Crossref] [PubMed]

2015 (2)

2014 (3)

2013 (2)

G. Zheng, R. Horstmeyer, and C. Yang, “Wide-field, high-resolution Fourier ptychographic microscopy,” Nat. Photonics 7(9), 739–745 (2013).
[Crossref] [PubMed]

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

2012 (3)

2011 (2)

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

2010 (4)

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

W. Bishara, T.-W. Su, A. F. Coskun, and A. Ozcan, “Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution,” Opt. Express 18(11), 11181–11191 (2010).
[Crossref] [PubMed]

2009 (1)

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

2008 (2)

S. Seo, T.-W. Su, A. Erlinger, and A. Ozcan, “Multi-color LUCAS: Lensfree On-chip Cytometry Using Tunable Monochromatic Illumination and Digital Noise Reduction,” Cell. Mol. Bioeng. 1(2–3), 146–156 (2008).
[Crossref]

M. Guizar-Sicairos, S. T. Thurman, and J. R. Fienup, “Efficient subpixel image registration algorithms,” Opt. Lett. 33(2), 156–158 (2008).
[Crossref] [PubMed]

2004 (1)

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

1996 (3)

1993 (1)

1987 (2)

Anand, A.

Barbastathis, G.

Bhaduri, B.

Bishara, W.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

W. Bishara, T.-W. Su, A. F. Coskun, and A. Ozcan, “Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution,” Opt. Express 18(11), 11181–11191 (2010).
[Crossref] [PubMed]

Bostan, E.

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

Chen, Q.

Cho, C.

Choi, B.

Claus, R. A.

Coskun, A. F.

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

W. Bishara, T.-W. Su, A. F. Coskun, and A. Ozcan, “Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution,” Opt. Express 18(11), 11181–11191 (2010).
[Crossref] [PubMed]

Courbin, F.

Dauwels, J.

Eberhardt, W.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Edwards, C.

Eisebitt, S.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Erlinger, A.

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

S. Seo, T.-W. Su, A. Erlinger, and A. Ozcan, “Multi-color LUCAS: Lensfree On-chip Cytometry Using Tunable Monochromatic Illumination and Digital Noise Reduction,” Cell. Mol. Bioeng. 1(2–3), 146–156 (2008).
[Crossref]

Feng, S.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

Ferrari, A.

Fienup, J. R.

Froustey, E.

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

Goddard, L. L.

Göröcs, Z.

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

Greenbaum, A.

W. Luo, A. Greenbaum, Y. Zhang, and A. Ozcan, “Synthetic aperture-based on-chip microscopy,” Light Sci. Appl. 4(3), e261 (2015).
[Crossref]

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

Griffin, B. G.

Guizar-Sicairos, M.

Hellwig, O.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Hennelly, B. M.

B. M. Hennelly, D. P. Kelly, N. Pandey, and D. Monaghan, “Review of Twin Reduction and Twin Removal Techniques in Holography,” in Proceedings of the China–Ireland Information and Communications Technologies Conference, National University of Ireland Maynooth, Ireland, (2009), pp. 241–245).

Horstmeyer, R.

G. Zheng, R. Horstmeyer, and C. Yang, “Wide-field, high-resolution Fourier ptychographic microscopy,” Nat. Photonics 7(9), 739–745 (2013).
[Crossref] [PubMed]

Hu, Y.

Isikman, S. O.

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

Javidi, B.

Jingshan, Z.

Joyeux, D.

Kang, H.

Kelly, D. P.

B. M. Hennelly, D. P. Kelly, N. Pandey, and D. Monaghan, “Review of Twin Reduction and Twin Removal Techniques in Holography,” in Proceedings of the China–Ireland Information and Communications Technologies Conference, National University of Ireland Maynooth, Ireland, (2009), pp. 241–245).

Khademhosseini, B.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

Khademhosseinieh, B.

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

Kim, T.

Koren, G.

Lau, R.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

Lee, K.

Lee, S.

Leitgeb, R. A.

Levene, M.

Liu, G.

Lörgen, M.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Luckhart, S.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

Lüning, J.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Luo, W.

W. Luo, A. Greenbaum, Y. Zhang, and A. Ozcan, “Synthetic aperture-based on-chip microscopy,” Light Sci. Appl. 4(3), e261 (2015).
[Crossref]

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

Mavandadi, S.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

Monaghan, D.

B. M. Hennelly, D. P. Kelly, N. Pandey, and D. Monaghan, “Review of Twin Reduction and Twin Removal Techniques in Holography,” in Proceedings of the China–Ireland Information and Communications Technologies Conference, National University of Ireland Maynooth, Ireland, (2009), pp. 241–245).

Mudanyali, O.

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

Nguyen, T.

Nilchian, M.

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

Oh, C.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

Ozcan, A.

W. Luo, A. Greenbaum, Y. Zhang, and A. Ozcan, “Synthetic aperture-based on-chip microscopy,” Light Sci. Appl. 4(3), e261 (2015).
[Crossref]

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

W. Bishara, T.-W. Su, A. F. Coskun, and A. Ozcan, “Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution,” Opt. Express 18(11), 11181–11191 (2010).
[Crossref] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

S. Seo, T.-W. Su, A. Erlinger, and A. Ozcan, “Multi-color LUCAS: Lensfree On-chip Cytometry Using Tunable Monochromatic Illumination and Digital Noise Reduction,” Cell. Mol. Bioeng. 1(2–3), 146–156 (2008).
[Crossref]

Oztoprak, C.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

Pandey, N.

B. M. Hennelly, D. P. Kelly, N. Pandey, and D. Monaghan, “Review of Twin Reduction and Twin Removal Techniques in Holography,” in Proceedings of the China–Ireland Information and Communications Technologies Conference, National University of Ireland Maynooth, Ireland, (2009), pp. 241–245).

Park, Y.

Pham, H.

Polack, F.

Popescu, G.

Psaltis, D.

Pu, A.

A. Pu and D. Psaltis, “High-density recording in photopolymer-based holographic three-dimensional disks,” Appl. Opt. 35(14), 2389–2398 (1996).
[Crossref] [PubMed]

A. Pu and M. Spie, “Exposure schedule for multiplexing holograms in photopolymer films,” Opt. Eng. 35, 2824–2829 (1996).

Sage, D.

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

Schlotter, W. F.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Schutz, A.

Scott, P. D.

Sencan, I.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

Seo, S.

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

S. Seo, T.-W. Su, A. Erlinger, and A. Ozcan, “Multi-color LUCAS: Lensfree On-chip Cytometry Using Tunable Monochromatic Illumination and Digital Noise Reduction,” Cell. Mol. Bioeng. 1(2–3), 146–156 (2008).
[Crossref]

Sikora, U.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

Singh, A. S. G.

Soulez, F.

Spie, M.

A. Pu and M. Spie, “Exposure schedule for multiplexing holograms in photopolymer films,” Opt. Eng. 35, 2824–2829 (1996).

Stöhr, J.

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Su, T.-W.

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
[Crossref]

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

W. Bishara, T.-W. Su, A. F. Coskun, and A. Ozcan, “Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution,” Opt. Express 18(11), 11181–11191 (2010).
[Crossref] [PubMed]

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

S. Seo, T.-W. Su, A. Erlinger, and A. Ozcan, “Multi-color LUCAS: Lensfree On-chip Cytometry Using Tunable Monochromatic Illumination and Digital Noise Reduction,” Cell. Mol. Bioeng. 1(2–3), 146–156 (2008).
[Crossref]

Sun, J.

Thiébaut, É.

Thurman, S. T.

Tian, L.

Tseng, D.

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

Tseng, D. K.

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

Unser, M.

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

F. Soulez, É. Thiébaut, A. Schutz, A. Ferrari, F. Courbin, and M. Unser, “Proximity operators for phase retrieval,” Appl. Opt. 55(26), 7412–7421 (2016).
[Crossref] [PubMed]

Waller, L.

Xue, L.

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

Yaglidere, O.

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

Yang, C.

G. Zheng, R. Horstmeyer, and C. Yang, “Wide-field, high-resolution Fourier ptychographic microscopy,” Nat. Photonics 7(9), 739–745 (2013).
[Crossref] [PubMed]

Yu, F. W.

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

Zhang, J.

Zhang, Y.

W. Luo, A. Greenbaum, Y. Zhang, and A. Ozcan, “Synthetic aperture-based on-chip microscopy,” Light Sci. Appl. 4(3), e261 (2015).
[Crossref]

Zheng, G.

G. Zheng, R. Horstmeyer, and C. Yang, “Wide-field, high-resolution Fourier ptychographic microscopy,” Nat. Photonics 7(9), 739–745 (2013).
[Crossref] [PubMed]

Zuo, C.

Appl. Opt. (3)

Cell. Mol. Bioeng. (1)

S. Seo, T.-W. Su, A. Erlinger, and A. Ozcan, “Multi-color LUCAS: Lensfree On-chip Cytometry Using Tunable Monochromatic Illumination and Digital Noise Reduction,” Cell. Mol. Bioeng. 1(2–3), 146–156 (2008).
[Crossref]

IEEE Trans. Image Process. (1)

E. Bostan, E. Froustey, M. Nilchian, D. Sage, and M. Unser, “Variational Phase Imaging Using the Transport-of-Intensity Equation,” IEEE Trans. Image Process. 25(2), 807–817 (2016).
[Crossref] [PubMed]

J. Opt. Soc. Am. A (3)

Lab Chip (4)

D. Tseng, O. Mudanyali, C. Oztoprak, S. O. Isikman, I. Sencan, O. Yaglidere, and A. Ozcan, “Lensfree microscopy on a cellphone,” Lab Chip 10(14), 1787–1792 (2010).
[Crossref] [PubMed]

S. Seo, T.-W. Su, D. K. Tseng, A. Erlinger, and A. Ozcan, “Lensfree holographic imaging for on-chip cytometry and diagnostics,” Lab Chip 9(6), 777–787 (2009).
[Crossref] [PubMed]

O. Mudanyali, D. Tseng, C. Oh, S. O. Isikman, I. Sencan, W. Bishara, C. Oztoprak, S. Seo, B. Khademhosseini, and A. Ozcan, “Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications,” Lab Chip 10(11), 1417–1428 (2010).
[Crossref] [PubMed]

W. Bishara, U. Sikora, O. Mudanyali, T.-W. Su, O. Yaglidere, S. Luckhart, and A. Ozcan, “Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array,” Lab Chip 11(7), 1276–1279 (2011).
[Crossref] [PubMed]

Light Sci. Appl. (1)

W. Luo, A. Greenbaum, Y. Zhang, and A. Ozcan, “Synthetic aperture-based on-chip microscopy,” Light Sci. Appl. 4(3), e261 (2015).
[Crossref]

Nat. Methods (1)

A. Greenbaum, W. Luo, T.-W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, and A. Ozcan, “Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy,” Nat. Methods 9(9), 889–895 (2012).
[Crossref] [PubMed]

Nat. Photonics (1)

G. Zheng, R. Horstmeyer, and C. Yang, “Wide-field, high-resolution Fourier ptychographic microscopy,” Nat. Photonics 7(9), 739–745 (2013).
[Crossref] [PubMed]

Nature (1)

S. Eisebitt, J. Lüning, W. F. Schlotter, M. Lörgen, O. Hellwig, W. Eberhardt, and J. Stöhr, “Lensless imaging of magnetic nanostructures by X-ray spectro-holography,” Nature 432(7019), 885–888 (2004).
[Crossref] [PubMed]

Opt. Eng. (1)

A. Pu and M. Spie, “Exposure schedule for multiplexing holograms in photopolymer films,” Opt. Eng. 35, 2824–2829 (1996).

Opt. Express (7)

T.-W. Su, S. O. Isikman, W. Bishara, D. Tseng, A. Erlinger, and A. Ozcan, “Multi-angle lensless digital holography for depth resolved imaging on a chip,” Opt. Express 18(9), 9690–9711 (2010).
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C. Zuo, J. Sun, J. Zhang, Y. Hu, and Q. Chen, “Lensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix,” Opt. Express 23(11), 14314–14328 (2015).
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Z. Jingshan, R. A. Claus, J. Dauwels, L. Tian, and L. Waller, “Transport of Intensity phase imaging by intensity spectrum fitting of exponentially spaced defocus planes,” Opt. Express 22(9), 10661–10674 (2014).
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C. Edwards, B. Bhaduri, T. Nguyen, B. G. Griffin, H. Pham, T. Kim, G. Popescu, and L. L. Goddard, “Effects of spatial coherence in diffraction phase microscopy,” Opt. Express 22(5), 5133–5146 (2014).
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C. Cho, B. Choi, H. Kang, and S. Lee, “Numerical twin image suppression by nonlinear segmentation mask in digital holography,” Opt. Express 20(20), 22454–22464 (2012).
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W. Bishara, T.-W. Su, A. F. Coskun, and A. Ozcan, “Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution,” Opt. Express 18(11), 11181–11191 (2010).
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A. S. G. Singh, A. Anand, R. A. Leitgeb, and B. Javidi, “Lateral shearing digital holographic imaging of small biological specimens,” Opt. Express 20(21), 23617–23622 (2012).
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Opt. Lett. (2)

Proc. Natl. Acad. Sci. U.S.A. (1)

S. O. Isikman, W. Bishara, S. Mavandadi, F. W. Yu, S. Feng, R. Lau, and A. Ozcan, “Lens-free optical tomographic microscope with a large imaging volume on a chip,” Proc. Natl. Acad. Sci. U.S.A. 108(18), 7296–7301 (2011).
[Crossref] [PubMed]

Sci. Rep. (1)

A. Greenbaum, W. Luo, B. Khademhosseinieh, T.-W. Su, A. F. Coskun, and A. Ozcan, “Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy,” Sci. Rep. 3, 1717 (2013).
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A. C. Sobieranski, F. Inci, H. C. Tekin, E. Comunello, A. Wangenheim, and U. Demirci, “Portable Digital in-Line Holography Platform for Sperm Cell Visualization and Quantification,” 2014 27th SIBGRAPI Conf. Graph. Patterns Images, 274–281 (2014).
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B. M. Hennelly, D. P. Kelly, N. Pandey, and D. Monaghan, “Review of Twin Reduction and Twin Removal Techniques in Holography,” in Proceedings of the China–Ireland Information and Communications Technologies Conference, National University of Ireland Maynooth, Ireland, (2009), pp. 241–245).

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[Crossref]

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Figures (4)

Fig. 1
Fig. 1 (a) 3D sketch of the prism with a laminated photopolymer film. (b) Side view showing multiplexing of two hologram gratings. Reference beam 1 and signal beam 1 interfere in the photopolymer film inducing index of refraction changes, which result in a phase volume grating. Then the prism is moved to have the reference beam at position 2 and the signal beam direction is changed to obtain angle 2. The two beams interfere in the photopolymer inducing another phase hologram grating.
Fig. 2
Fig. 2 2D sketch of the compact phase imager with a side illumination system composed of VCSELs illuminating the multiplexed hologram on a photopolymer (fabricated according to Fig. 1 (b). For clarity only two angles of illumination out of 9, corresponding to two VCSELs, are shown. All the beams overlap on a ~17mm2 FOV, corresponding to ~50% of the camera chip size.
Fig. 3
Fig. 3 (a) Normalized diffraction efficiency versus source position (φ = −11.5°, θ = −11.5°). The experimental curve is broader than what is expected by the theory. The deviation between the simulation and the measurement come from the effective thickness of photopolymer used to record the grating, which is smaller than the expected 70μm. (b) Diffraction efficiencies of 9 multiplexed hologram gratings versus source position along the entrance surface of the prism.
Fig. 4
Fig. 4 (a) Reconstructed phase with the proposed device and algorithm of the full FOV. Halos in the large flat regions are due to a noisy estimation of low frequencies phase. This effect is also present in transport of intensity experiments and other quantitative phase imaging techniques (b) Reconstructed phase using only one hologram backpropagated with the angular spectrum method (crop of 1.1x1.1mm). (c) Reconstructed phase with the proposed device and algorithm (crop of 1.1x1.1mm). (d) Reconstructed phase with a Digital Holographic Microscope (DHM) (crop of 1.1x1.1mm).

Equations (1)

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φ = arctan( xp z ), θ = arctan( yp z )

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