Abstract

Multiplexed imaging is a powerful tool for studying complex interactions inside biological systems. Spectral imaging methods that capture multiple fluorescent markers synchronously without sacrificing the imaging speed or resolution are most suitable for live imaging. We describe spectral-encoded structured illumination (spectral-SIM) light-sheet microscopy, which enables parallel multi-excitation-channel imaging in 3D. Spectral-SIM encodes the excitation wavelength as the phase of the illumination pattern, and allows synchronous image capture over multiple excitation channels at the same speed and spatial resolution as mono-channel structured light-sheet imaging. The technique retains structured light-sheet microscopy’s ability in removing out-of-focus and scattered emission background, and generates clear 3D multiplexed images in thick tissue. The capability of this technique was demonstrated by the imaging of live triple-labeled transgenic zebrafish to over 300 μm deep with 0.5μm-by-2μm (lateral-by-axial) resolution.

© 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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  1. J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods 8, 811–819 (2011).
    [Crossref] [PubMed]
  2. J. Huisken, “Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy,” Science 305, 1007–1009 (2004).
    [Crossref] [PubMed]
  3. P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
    [Crossref] [PubMed]
  4. T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
    [Crossref] [PubMed]
  5. U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
    [Crossref] [PubMed]
  6. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
    [Crossref] [PubMed]
  7. T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
    [Crossref] [PubMed]
  8. P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
    [Crossref] [PubMed]
  9. B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
    [Crossref] [PubMed]
  10. M. Zhao, H. Zhang, Y. Li, A. Ashok, R. Liang, W. Zhou, and L. Peng, “Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy,” Biomed. Opt. Express 5, 1296–1308 (2014).
    [Crossref] [PubMed]
  11. L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
    [Crossref] [PubMed]
  12. G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
    [Crossref] [PubMed]
  13. P. Zhang, M. E. Phipps, P. M. Goodwin, and J. H. Werner, “Confocal line scanning of a Bessel beam for fast 3D imaging,” Opt. Lett. 39, 3682–3685 (2014).
    [Crossref] [PubMed]
  14. T. T. Zimmermann, J. J. Rietdorf, and R. R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546, 87–92 (2003).
    [Crossref] [PubMed]
  15. P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
    [Crossref] [PubMed]
  16. Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
    [Crossref] [PubMed]
  17. E. Baumgart and U. Kubitscheck, “Scanned light sheet microscopy with confocal slit detection,” Opt. Express 20, 21805–21814 (2012).
    [Crossref] [PubMed]
  18. W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
    [Crossref]
  19. M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198, 82 (2000).
    [Crossref] [PubMed]
  20. M. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” PNAS 102, 13081–13086 (2005).
    [Crossref] [PubMed]
  21. M. A. Neil, R. Juskaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Opt. Lett. 22, 1905–1907 (1997).
    [Crossref]
  22. S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
    [Crossref]
  23. D. Li and E. Betzig, “Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
    [Crossref]
  24. Y. Garini, I. T. Young, and G. McNamara, “Spectral Imaging: Principles and Applications,” Cytom. Part A 69A, 735–747 (2006).
    [Crossref]
  25. F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
    [Crossref] [PubMed]
  26. J. Leach, G. M. Gibson, M. J. Padgett, E. Esposito, G. McConnell, A. J. Wright, and J. M. Girkin, “Generation of achromatic Bessel beams using a compensated spatial light modulator,” Opt. Express 14, 5581–5587 (2006).
    [Crossref] [PubMed]
  27. D. Xu, W. Zhou, and L. Peng, “Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam,” Biomed. Opt. Express 8, 570–578 (2017).
    [Crossref] [PubMed]
  28. A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
    [Crossref] [PubMed]
  29. F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21, 21010–21026 (2013).
    [Crossref] [PubMed]

2017 (2)

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

D. Xu, W. Zhou, and L. Peng, “Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam,” Biomed. Opt. Express 8, 570–578 (2017).
[Crossref] [PubMed]

2016 (3)

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

D. Li and E. Betzig, “Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

2015 (2)

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

2014 (5)

M. Zhao, H. Zhang, Y. Li, A. Ashok, R. Liang, W. Zhou, and L. Peng, “Cellular imaging of deep organ using two-photon Bessel light-sheet nonlinear structured illumination microscopy,” Biomed. Opt. Express 5, 1296–1308 (2014).
[Crossref] [PubMed]

P. Zhang, M. E. Phipps, P. M. Goodwin, and J. H. Werner, “Confocal line scanning of a Bessel beam for fast 3D imaging,” Opt. Lett. 39, 3682–3685 (2014).
[Crossref] [PubMed]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
[Crossref] [PubMed]

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

2013 (1)

2012 (4)

E. Baumgart and U. Kubitscheck, “Scanned light sheet microscopy with confocal slit detection,” Opt. Express 20, 21805–21814 (2012).
[Crossref] [PubMed]

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

2011 (3)

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods 8, 811–819 (2011).
[Crossref] [PubMed]

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

2010 (1)

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

2008 (1)

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
[Crossref] [PubMed]

2006 (2)

2005 (1)

M. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” PNAS 102, 13081–13086 (2005).
[Crossref] [PubMed]

2004 (1)

J. Huisken, “Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

2003 (1)

T. T. Zimmermann, J. J. Rietdorf, and R. R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546, 87–92 (2003).
[Crossref] [PubMed]

2000 (1)

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198, 82 (2000).
[Crossref] [PubMed]

1997 (1)

Albert, M.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Artiga, M. S.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

Ashok, A.

Balzarotti, F.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Bao, Z.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

Baumgart, E.

Beaurepaire, E.

P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
[Crossref] [PubMed]

Bembenek, J. N.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Betzig, E.

D. Li and E. Betzig, “Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Bohme, R.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Chen, B. C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Chiu, C.-L.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

Chmyrov, A.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Choi, J. M.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

Cizmar, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Coll-Llado, C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Cutrale, F.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

Dalgarno, H. I. C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Davidson, M. W.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Dholakia, K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Dwight, J.

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Egner, A.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

English, B. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Esposito, E.

Fahrbach, F. O.

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21, 21010–21026 (2013).
[Crossref] [PubMed]

Ferrier, D. E. K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Fiuza, U.-M.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Fraser, S. E.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

Fritz-Laylin, L.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Galbraith, C. G.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Galbraith, J. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Gao, L.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Garini, Y.

Y. Garini, I. T. Young, and G. McNamara, “Spectral Imaging: Principles and Applications,” Cytom. Part A 69A, 735–747 (2006).
[Crossref]

Gibson, G. M.

Girkin, J. M.

Goldstein, B.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Goodwin, P. M.

Grill, S. W.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Grotjohann, T.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Gunn-Moore, F. J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Gunther, S.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

Gustafsson, M.

M. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” PNAS 102, 13081–13086 (2005).
[Crossref] [PubMed]

Gustafsson, M. G. L.

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198, 82 (2000).
[Crossref] [PubMed]

Hammer, J. A.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Helmchen, F.

Higgins, C. D.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Hiiragi, T.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Hufnagel, L.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

Huisken, J.

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21, 21010–21026 (2013).
[Crossref] [PubMed]

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[Crossref] [PubMed]

J. Huisken, “Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Jahr, W.

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

Jakobs, S.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Janetopoulos, C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Juskaitis, R.

Kaufmann, A.

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[Crossref] [PubMed]

Keller, P. J.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
[Crossref] [PubMed]

Keller-Findeisen, J.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Khairy, K.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

Kiehart, D. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Koos, D. S.

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

Krzic, U.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

Kubitscheck, U.

Lavagnino, Z.

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Lavoie-Cardinal, F.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Leach, J.

Legant, W. R.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Leutenegger, M.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Li, D.

D. Li and E. Betzig, “Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Li, Y.

Liang, R.

Lippincott-Schwartz, J.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Liu, Z.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Mahou, P.

P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
[Crossref] [PubMed]

McConnell, G.

McNamara, G.

Y. Garini, I. T. Young, and G. McNamara, “Spectral Imaging: Principles and Applications,” Cytom. Part A 69A, 735–747 (2006).
[Crossref]

Medeiros, G. d.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Mertz, J.

J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods 8, 811–819 (2011).
[Crossref] [PubMed]

Mickoleit, M.

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[Crossref] [PubMed]

Milkie, D. E.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Mimori-Kiyosue, Y.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Mitchell, D. M.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Mullins, R. D.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Neil, M. A.

Nguyen, T.-U.

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Norlin, N.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Nylk, J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Padgett, M. J.

Panavaite, L.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Peifer, M.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Peng, L.

Pepperkok, R. R.

T. T. Zimmermann, J. J. Rietdorf, and R. R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546, 87–92 (2003).
[Crossref] [PubMed]

Peri, F.

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Phipps, M. E.

Piston, D. W.

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Planchon, T. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Poulton, J. S.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Reymann, A. C.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Rietdorf, J. J.

T. T. Zimmermann, J. J. Rietdorf, and R. R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546, 87–92 (2003).
[Crossref] [PubMed]

Ritter, A. T.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Romero, D. P.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Sahl, S. J.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Santella, A.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

Saunders, T. E.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

Schmid, B.

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21, 21010–21026 (2013).
[Crossref] [PubMed]

Schmidt, A. D.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
[Crossref] [PubMed]

Schmied, C.

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

Seydoux, G.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Shao, L.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Stelzer, E. H. K.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
[Crossref] [PubMed]

Streichan, S. J.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

Supatto, W.

P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
[Crossref] [PubMed]

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

Tkaczyk, T. S.

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Trinh, L. A.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

Trivedi, V.

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

Truong, T. V.

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

Tulu, U. S.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Ustione, A.

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Vermot, J.

P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
[Crossref] [PubMed]

Vettenburg, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Voigt, F. F.

Wang, J. T.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Wang, K.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Weber, M.

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[Crossref] [PubMed]

Werner, J. H.

Westphal, V.

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

Wilson, T.

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
[Crossref] [PubMed]

Wright, A. J.

Wu, X.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Wu, X. S.

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

Xu, D.

Young, I. T.

Y. Garini, I. T. Young, and G. McNamara, “Spectral Imaging: Principles and Applications,” Cytom. Part A 69A, 735–747 (2006).
[Crossref]

Zhang, H.

Zhang, P.

Zhao, M.

Zhou, W.

Zimmermann, T. T.

T. T. Zimmermann, J. J. Rietdorf, and R. R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546, 87–92 (2003).
[Crossref] [PubMed]

Biomed. Opt. Express (2)

Biophys. J. (1)

Z. Lavagnino, J. Dwight, A. Ustione, T.-U. Nguyen, T. S. Tkaczyk, and D. W. Piston, “Snapshot Hyperspectral Light-Sheet Imaging of Signal Transduction in Live Pancreatic Islets,” Biophys. J. 111, 409–417 (2016).
[Crossref] [PubMed]

Cell (1)

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens,” Cell 151, 1370–1385 (2012).
[Crossref] [PubMed]

Cytom. Part A (1)

Y. Garini, I. T. Young, and G. McNamara, “Spectral Imaging: Principles and Applications,” Cytom. Part A 69A, 735–747 (2006).
[Crossref]

Development (1)

A. Kaufmann, M. Mickoleit, M. Weber, and J. Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[Crossref] [PubMed]

FEBS Lett. (1)

T. T. Zimmermann, J. J. Rietdorf, and R. R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett. 546, 87–92 (2003).
[Crossref] [PubMed]

J. Microsc. (1)

M. G. L. Gustafsson, “Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy,” J. Microsc. 198, 82 (2000).
[Crossref] [PubMed]

Nat. Commun. (2)

W. Jahr, B. Schmid, C. Schmied, F. O. Fahrbach, and J. Huisken, “Hyperspectral light sheet microscopy,” Nat. Commun. 6, 7790 (2015).
[Crossref]

G. d. Medeiros, N. Norlin, S. Gunther, M. Albert, L. Panavaite, U.-M. Fiuza, F. Peri, T. Hiiragi, U. Krzic, and L. Hufnagel, “Confocal multiview light-sheet microscopy,” Nat. Commun. 6, 8881 (2015).
[Crossref] [PubMed]

Nat. Methods (8)

F. Cutrale, V. Trivedi, L. A. Trinh, C.-L. Chiu, J. M. Choi, M. S. Artiga, and S. E. Fraser, “Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging,” Nat. Methods 14, 149–152 (2017).
[Crossref] [PubMed]

P. Mahou, J. Vermot, E. Beaurepaire, and W. Supatto, “Multicolor two-photon light-sheet microscopy,” Nat. Methods 11, 600–601 (2014).
[Crossref] [PubMed]

J. Mertz, “Optical sectioning microscopy with planar or structured illumination,” Nat. Methods 8, 811–819 (2011).
[Crossref] [PubMed]

T. V. Truong, W. Supatto, D. S. Koos, J. M. Choi, and S. E. Fraser, “Deep and fast live imaging with two-photon scanned light-sheet microscopy,” Nat. Methods 8, 757 (2011).
[Crossref] [PubMed]

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9, 730–733 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Cizmar, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, A. Santella, K. Khairy, Z. Bao, J. Wittbrodt, and E. H. K. Stelzer, “Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy,” Nat. Methods 7, 637–642 (2010).
[Crossref] [PubMed]

Opt. Express (3)

Opt. Lett. (2)

PNAS (1)

M. Gustafsson, “Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution,” PNAS 102, 13081–13086 (2005).
[Crossref] [PubMed]

Science (5)

S. J. Sahl, F. Balzarotti, J. Keller-Findeisen, M. Leutenegger, V. Westphal, A. Egner, F. Lavoie-Cardinal, A. Chmyrov, T. Grotjohann, and S. Jakobs, “Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

D. Li and E. Betzig, “Response to Comment on “Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics”,” Science 352, 527 (2016).
[Crossref]

B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Ritter, J. Lippincott-Schwartz, L. Fritz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, and E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).
[Crossref] [PubMed]

J. Huisken, “Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy,” Science 322, 1065–1069 (2008).
[Crossref] [PubMed]

Supplementary Material (2)

NameDescription
» Visualization 1       3D rendered triple excitation channel image stack of live Tg(kdrl:GFP; fli1a:Gal4; UAS:nfsB-mCherry) embryo stained by Syto60 at 2dpf
» Visualization 2       Slice fly-through of triple excitation channel image stack of live Tg(kdrl:GFP; fli1a:Gal4; UAS:nfsB-mCherry) embryo stained by Syto60 at 2dpf

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Figures (7)

Fig. 1
Fig. 1 Simulation comparison of uniform light-sheet, confocal slit-scanning light-sheet and SIM light-sheet. The simulation assumes the image objective lens has NA =0.8, and the digital camera has an equivalent pixels size of 0.25 μm. The laser line has a width of 2 μm, which is typical in light-sheet imaging. The step size of SIM pattern shift is 1 μm. Poisson noise was added to simulated raw images. Scale bars: 10 μm. (a) The high-resolution target used for simulation. The confocal slit method and SIM methods are simulated under the same total photon budget and the same degree of signal diffusion. (b) Image taken with a uniform light-sheet in the present of strong scattering. The simulation assumes 90% of the signal is scattered and forms a diffused background. (c–f) Confocal scanning-slit light sheet imaging. The laser line illumination c sweeps across the field in synchronization with the confocal slit and generates a diffused line-shape image on the camera d. The effect of slit-detection is simulated by keeping signal within the slit area (red rectangle) and zeroing out signal outside of the slit area. The final image was the sum of multiple frames simulated with the sweeping laser line. A wide slit of 2 μm results in a brighter image e, but more background signal is leaked into the image. The image simulated with a 0.25 μm slit width f contains less background signal, but the desired image signal also drops significantly. (g–i) Light-sheet imaging with the linear SIM (3-frame) method. Multiple laser lines are spaced in narrow spacing within the field, forming a periodic illumination pattern g. After taken 3 frames wide-field exposures h under shifted illumination patterns, SIM image processing yields an image i that is background-free. (j–l) Light-sheet imaging with the NSIM method. Using a wide-spaced periodic illumination pattern j and 11-frame exposures k, the resulting image l has higher signals and better SNR than the 3-frame result, and is still mostly background-free. (m) Image signal detection efficiency and background signal leaking percentage vs. the slit width in the confocal slit method. (n) Image signal detection efficiency and background signal leaking percentage vs. the step number of NSIM.
Fig. 2
Fig. 2 Removing the residue background signal in NSIM. (a) In Fourier image n(kx, ky), the desired in-focus and un-scattered signal is shifted in frequency, but residue of the background signal remains near the zero frequency. (b) Numerically shifting the image back to the original frequency and applying a half-sided filter will generate a background-free image.
Fig. 3
Fig. 3 Simulation results demonstrating the spectral-SIM. (a) The object has three labels, shown in red, green and blue pseudo colors. The nonlinear excitation pattern is the superposition of three excitation patterns, each having the same spatial period but a different initial phase and exciting a label shown in the matching pseudo color. (b) An example of I (x, y, ϕi), the raw spatial image obtained under the triple-excitation structure illumination and the absolute value of its Fourier image, obtained through 2D FFT. (c) Complex images IFFT [R̃″n(kx, ky)] whose absolute values represents intensities (left) and phases contain encoded excitation spectral information (right). (d) Images of each excitation channel after wavelength decoding (left). The merged pseudo image (right) matches with the original object used for simulation.
Fig. 4
Fig. 4 Schematics of a Bessel spectral-SIM light-sheet microscope. (a) The beam scanning configuration of Bessel spectral-SIM light-sheet microscope. The camera plane (not shown in the schematics) is parallel to the paper plane. SLM, spatial light modulator. (b) Illumination pattern captured with the EMCCD camera. The grating pair introduces a linear shifting between each different laser lines. The actual illumination pattern used in the imaging is the superposition of multiple excitation patterns.
Fig. 5
Fig. 5 Spectral-SIM processing of dual excitation-channel (488 nm and 561 nm) image. Raw images were taken from a caudal trunk of Tg(kdrl:GFP; fli1a:Gal4; UAS:nfsB-mCherry) triple transgenic fish (ventral right, dorsal left). GFP and mCherry were co-expressed in vascular endothelial cells. The SIM pattern period was 14 μm. 11 step exposures were used. Scale bar: 20 μm. (a) Reconstructed zero-order component image, IFFT [R̃′0(kx, ky)]. contains both in-focus ballistic signals and out-of-focus and scattered signals. (b) The absolute value of the complex image, IFFT [R̃′1(kx, ky)], is background free. (c) The phase value of the complex image, IFFT [R̃′1(kx, ky)], encodes the sources of excitation. The arrow points to an area with a phase different from the majority of the map. (d) The dual excitation image represented in pseudo colors (green: emission from 488 nm laser, mainly emitted by GFP; red: emission from 561 nm laser, mainly emitted by mCherry). The image was obtained by spectral decoding Rn(x, y). The same area pointed by the arrow has stronger 488nm excitation (stronger GFP expression) than the rest of the image.
Fig. 6
Fig. 6 Reconstructed dual-excitation-channel images taken with one laser off. Results show negligible channel bleedthrough. Scale bar: 20 μm.
Fig. 7
Fig. 7 3D triple excitation channel imaging of live Tg(kdrl:GFP; fli1a:Gal4; UAS:nfsB-mCherry) embryo stained by Syto60 at 2dpf. 3D Rendered spectral-SIM image and slice fly-through movie can be viewed in Visualization 1 and Visualization 2. The SIM pattern period was 14 μm. 11 step exposures were used. Scale bar: 20 μm. (a) 3D rendered mono-channel image taken with a plane illumination. Arrows mark cross section locations. (b) Cross section at 126 μm deep, showing a strong background that blurs fine structures. (c) Cross section of vasculature at 181 μm deep. (d) Lateral cross section. (e) 3D rendered triple-excitation-channel image taken with spectral-SIM. The image is free of diffused background. Blue: 640 nm excitation, mostly emitted by Syto60. Green: 488 nm excitation, mostly emitted by GFP emission. Red: 561 excitation, mostly emitted by mCherry. (f) Cross section of 3D spectral-SIM image at 126 μm deep, showing Syto60 stained nasal epithelium cells can be excited by both 561 nm and 640 nm laser lines. The image is free of diffused background. (g) Cross section of vasculature, captured by spectral-SIM at 181 μm deep, showing co-expressed GFP and mCherry, excited by 488 and 561 nm respectively. (h) Lateral cross section of 3D spectral-SIM image.

Equations (15)

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I ex ( x , y , ϕ i ) = f [ cos ( k 0 x + a ϕ i + ϕ 0 ) ]
I ex ( x , y , ϕ i ) = n = N , N a n exp ( in k 0 x + in ϕ i + in ϕ 0 )
I ( x , y , ϕ i ) = O ( x , y ) I ex ( x , y , ϕ i ) PSF ( x , y )
I ˜ ( k x , k y , ϕ i ) = n = N , N R ˜ n ( k x , k y ) exp ( in ϕ i )
R ˜ n ( k x , k y ) = a n O ˜ ( k x + n k 0 , k y ) exp ( in ϕ 0 ) × OTF ( k x , k y ) .
R ˜ n ( k x , k y ) = a n O ˜ ( k x , k y ) exp ( in ϕ 0 ) × OTF ( k x n k 0 , k y )
R ˜ n ( k x , k y ) a n O ˜ ( k x , k y ) exp ( in ϕ 0 ) × OTF ( k x , k y )
I ex ( x , y , ϕ i ) = λ n = N , N a n I λ exp [ in k 0 x + in ϕ i + in ϕ ( λ ) ]
I ˜ ( x , y , ϕ i ) = λ O ( x , y , λ ) I ex ( x , y , ϕ i , λ ) PSF ( x , y )
R ˜ n ( k x , k y ) a n λ O ˜ ( k x , k y , λ ) exp [ in ϕ ( λ ) ] × OTF ( k x , k y )
I ( x , y , ϕ i ) = λ O ( x , y , λ ) I ex ( x , y , ϕ i , λ ) PSF ( x , y ) + λ O BG ( x , y , λ ) I ex ( x , y , ϕ i , λ ) D ( x , y ) PSF ( x , y )
R ˜ n ( k x , k y ) = a n λ O ˜ ( k x + n k 0 , k y , λ ) exp [ in ϕ ( λ ) ] × OTF ( k x , k y ) + a n λ O ˜ BG ( k x + n k 0 , k y , λ ) exp [ in ϕ ( λ ) ] × D ˜ ( k x , k y ) × OTF ( k x , k y )
R ˜ n ( k x , k y ) a n λ O ˜ ( k x + k y , λ ) exp [ in ϕ ( λ ) ] × OTF ( k x , k y ) + a n λ O ˜ BG ( k x , k y , λ ) exp [ in ϕ ( λ ) ] × D ˜ ( k x , n k 0 , k y ) × OTF ( k x , k y )
R ˜ n ( k x , k y ) = { R ˜ n ( k x , k y ) u ( k x ) , when n > 0 R ˜ n ( k x , k y ) u ( k x ) , when n < 0 { a n λ O ˜ ( k x , k y , λ ) exp [ in ϕ ( λ ) ] u ( k x ) OTF ( k x , k y ) when n > 0 a n λ O ˜ ( k x , k y , λ ) exp [ in ϕ ( λ ) ] u ( k x ) OTF ( k x , k y ) when n < 0
R n ( x , y ) = { Re { IFFT [ R ˜ n ( k x , k y ) ] } , when n 0 STD i [ I ( x , y , ϕ i ) ] , when n = 0 a n λ O ( x , y , λ ) cos [ n ϕ ( λ ) ] PSF ( x , y )

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