Abstract

A wide-angle 2D light scattering static cytometer is developed for the automatic classification of label-free acute and chronic myeloid leukemic cells. This wide-angle cytometer collects 2D light scattering signals in the angular range from 26 to 154 degrees, which is validated by good agreement between experimental and simulated 2D light scattering patterns from standard microspheres. By combining gray level differential statistics (GLDS) algorithm with support vector machine (SVM) for the 2D light scattering pattern analysis, HL-60 cells (acute myeloid leukemic cells) and K562 cells (chronic myeloid leukemic cells) can be automatically identified with a sensitivity of 92% and a specificity of 95%. Our wide-angle 2D light scattering label-free static cytometry may serve as a new method for the diagnosis and classification of leukemia subtypes.

© 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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References

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2017 (1)

R. L. Siegel, K. D. Miller, and A. Jemal, “Cancer Statistics, 2017,” CA Cancer J. Clin. 67(1), 7–30 (2017).
[PubMed]

2016 (7)

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

M. Lin, X. Qiao, Q. Liu, C. Shao, and X. Su, “Light-sheet-based 2D light scattering cytometry for label-free characterization of senescent cells,” Biomed. Opt. Express 7(12), 5170–5181 (2016).
[PubMed]

H. Shahin, M. Gupta, A. Janowska-Wieczorek, W. Rozmus, and Y. Y. Tsui, “Physical characterization of hematopoietic stem cells using multidirectional label-free light scatterings,” Opt. Express 24(25), 28877–28888 (2016).
[PubMed]

K. V. Gilev, M. A. Yurkin, E. S. Chernyshova, D. I. Strokotov, A. V. Chernyshev, and V. P. Maltsev, “Mature red blood cells: from optical model to inverse light-scattering problem,” Biomed. Opt. Express 7(4), 1305–1310 (2016).
[PubMed]

L. Zhang, X. Zhao, Z. Zhang, H. Zhao, W. Chen, and L. Yuan, “Relation between clinical mature and immature lymphocyte cells in human peripheral blood and their spatial label free scattering patterns,” Rev. Sci. Instrum. 87(7), 074301 (2016).
[PubMed]

L. Xie, Q. Liu, C. Shao, and X. Su, “Differentiation of normal and leukemic cells by 2D light scattering label-free static cytometry,” Opt. Express 24(19), 21700–21707 (2016).
[PubMed]

W. Moutier, L. Duforêt-Gaurier, M. Thyssen, H. Loisel, X. Mériaux, L. Courcot, D. Dessailly, and S. Alvain, “Scattering of individual particles from cytometry: tests on phytoplankton cultures,” Opt. Express 24(21), 24188–24212 (2016).
[PubMed]

2015 (3)

X. Su, S. Liu, X. Qiao, Y. Yang, K. Song, and B. Kong, “Pattern recognition cytometry for label-free cell classification by 2D light scattering measurements,” Opt. Express 23(21), 27558–27565 (2015).
[PubMed]

L. Xie, Y. Yang, X. Sun, X. Qiao, Q. Liu, K. Song, B. Kong, and X. Su, “2D light scattering static cytometry for label-free single cell analysis with submicron resolution,” Cytometry A 87(11), 1029–1037 (2015).
[PubMed]

M. C. Béné, D. Grimwade, C. Haferlach, T. Haferlach, G. Zini, and L. European, “Leukemia diagnosis: today and tomorrow,” Eur. J. Haematol. 95(4), 365–373 (2015).
[PubMed]

2014 (2)

2013 (1)

2012 (1)

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

2011 (4)

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

X. Su, S. E. Kirkwood, M. Gupta, L. Marquez-Curtis, Y. Qiu, A. Janowska-Wieczorek, W. Rozmus, and Y. Y. Tsui, “Microscope-based label-free microfluidic cytometry,” Opt. Express 19(1), 387–398 (2011).
[PubMed]

C. Greiner, M. Hunter, P. Huang, F. Rius, and I. Georgakoudi, “Confocal Backscattering Spectroscopy for Leukemic and Normal Blood Cell Discrimination,” Cytometry A 79(10), 866–873 (2011).
[PubMed]

A. Hsiao, M. Hunter, C. Greiner, S. Gupta, and I. Georgakoudi, “Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy,” J. Biomed. Opt. 16(3), 037007 (2011).
[PubMed]

2010 (1)

2008 (1)

F. E. Craig and K. A. Foon, “Flow cytometric immunophenotyping for hematologic neoplasms,” Blood 111(8), 3941–3967 (2008).
[PubMed]

2007 (2)

X. T. Su, C. Capjack, W. Rozmus, and C. Backhouse, “2D light scattering patterns of mitochondria in single cells,” Opt. Express 15(17), 10562–10575 (2007).
[PubMed]

S. An, W. Liu, and S. Venkatesh, “Fast cross-validation algorithms for least squares support vector machine and kernel ridge regression,” Pattern Recognit. 40(8), 2154–2162 (2007).

2004 (1)

D. Watson, N. Hagen, J. Diver, P. Marchand, and M. Chachisvilis, “Elastic light scattering from single cells: orientational dynamics in optical trap,” Biophys. J. 87(2), 1298–1306 (2004).
[PubMed]

2002 (1)

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular organization and substructure measured using angle-resolved low-coherence interferometry,” Biophys. J. 82(4), 2256–2264 (2002).
[PubMed]

2000 (1)

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

1999 (3)

R. Drezek, A. Dunn, and R. Richards-Kortum, “Light scattering from cells: finite-difference time-domain simulations and goniometric measurements,” Appl. Opt. 38(16), 3651–3661 (1999).
[PubMed]

D. Campana and E. Coustan-Smith, “Detection of minimal residual disease in acute leukemia by flow cytometry,” Cytometry 38(4), 139–152 (1999).
[PubMed]

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

1998 (2)

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

J. R. Mourant, J. P. Freyer, A. H. Hielscher, A. A. Eick, D. Shen, and T. M. Johnson, “Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics,” Appl. Opt. 37(16), 3586–3593 (1998).
[PubMed]

1993 (1)

J. Shao, “Linear-model selection by cross-validation,” J. Am. Stat. Assoc. 88(422), 486–494 (1993).

1975 (1)

G. C. Salzman, J. M. Crowell, J. C. Martin, T. T. Trujillo, A. Romero, P. F. Mullaney, and P. M. LaBauve, “Cell classification by laser light scattering: identification and separation of unstained leukocytes,” Acta Cytol. 19(4), 374–377 (1975).
[PubMed]

Alvain, S.

An, S.

S. An, W. Liu, and S. Venkatesh, “Fast cross-validation algorithms for least squares support vector machine and kernel ridge regression,” Pattern Recognit. 40(8), 2154–2162 (2007).

Arendt, J. T.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Arifler, D.

Arnold, R.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Backhouse, C.

Backman, V.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular organization and substructure measured using angle-resolved low-coherence interferometry,” Biophys. J. 82(4), 2256–2264 (2002).
[PubMed]

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Badizadegan, K.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular organization and substructure measured using angle-resolved low-coherence interferometry,” Biophys. J. 82(4), 2256–2264 (2002).
[PubMed]

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Baldoni, S.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Bardi, A.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Bassan, R.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Béné, M. C.

M. C. Béné, D. Grimwade, C. Haferlach, T. Haferlach, G. Zini, and L. European, “Leukemia diagnosis: today and tomorrow,” Eur. J. Haematol. 95(4), 365–373 (2015).
[PubMed]

Bergmann, L.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Bloomfield, C. D.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Boone, C. W.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular organization and substructure measured using angle-resolved low-coherence interferometry,” Biophys. J. 82(4), 2256–2264 (2002).
[PubMed]

Bullinger, L.

V. Shivarov and L. Bullinger, “Expression profiling of leukemia patients: Key lessons and future directions,” Exp. Hematol. 42(8), 651–660 (2014).
[PubMed]

Campana, D.

D. Campana and E. Coustan-Smith, “Detection of minimal residual disease in acute leukemia by flow cytometry,” Cytometry 38(4), 139–152 (1999).
[PubMed]

Capjack, C.

Cavazzini, F.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Chachisvilis, M.

D. Watson, N. Hagen, J. Diver, P. Marchand, and M. Chachisvilis, “Elastic light scattering from single cells: orientational dynamics in optical trap,” Biophys. J. 87(2), 1298–1306 (2004).
[PubMed]

Chan, J.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Chen, W.

L. Zhang, X. Zhao, Z. Zhang, H. Zhao, W. Chen, and L. Yuan, “Relation between clinical mature and immature lymphocyte cells in human peripheral blood and their spatial label free scattering patterns,” Rev. Sci. Instrum. 87(7), 074301 (2016).
[PubMed]

Chernyshev, A. V.

K. V. Gilev, M. A. Yurkin, E. S. Chernyshova, D. I. Strokotov, A. V. Chernyshev, and V. P. Maltsev, “Mature red blood cells: from optical model to inverse light-scattering problem,” Biomed. Opt. Express 7(4), 1305–1310 (2016).
[PubMed]

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Chernyshova, E. S.

Cibien, F.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Ciccone, M.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Courcot, L.

Coustan-Smith, E.

D. Campana and E. Coustan-Smith, “Detection of minimal residual disease in acute leukemia by flow cytometry,” Cytometry 38(4), 139–152 (1999).
[PubMed]

Craig, F. E.

F. E. Craig and K. A. Foon, “Flow cytometric immunophenotyping for hematologic neoplasms,” Blood 111(8), 3941–3967 (2008).
[PubMed]

Crawford, J. M.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Crowell, J. M.

G. C. Salzman, J. M. Crowell, J. C. Martin, T. T. Trujillo, A. Romero, P. F. Mullaney, and P. M. LaBauve, “Cell classification by laser light scattering: identification and separation of unstained leukocytes,” Acta Cytol. 19(4), 374–377 (1975).
[PubMed]

Cuneo, A.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Dalsass, A.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Dasari, R. R.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular organization and substructure measured using angle-resolved low-coherence interferometry,” Biophys. J. 82(4), 2256–2264 (2002).
[PubMed]

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

De Carolis, L.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Dessailly, D.

Di Raimondo, F.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Diebold, J.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Diver, J.

D. Watson, N. Hagen, J. Diver, P. Marchand, and M. Chachisvilis, “Elastic light scattering from single cells: orientational dynamics in optical trap,” Biophys. J. 87(2), 1298–1306 (2004).
[PubMed]

Dong, L.

Drezek, R.

Duforêt-Gaurier, L.

Dugas, M.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Dunn, A.

Eick, A. A.

European, L.

M. C. Béné, D. Grimwade, C. Haferlach, T. Haferlach, G. Zini, and L. European, “Leukemia diagnosis: today and tomorrow,” Eur. J. Haematol. 95(4), 365–373 (2015).
[PubMed]

Fabbiano, F.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Falini, B.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Fanin, R.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Farinelli, L.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Feld, M. S.

A. Wax, C. Yang, V. Backman, K. Badizadegan, C. W. Boone, R. R. Dasari, and M. S. Feld, “Cellular organization and substructure measured using angle-resolved low-coherence interferometry,” Biophys. J. 82(4), 2256–2264 (2002).
[PubMed]

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Feng, Y.

Fitzmaurice, M.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Flandrin, G.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Follen, M.

Foon, K. A.

F. E. Craig and K. A. Foon, “Flow cytometric immunophenotyping for hematologic neoplasms,” Blood 111(8), 3941–3967 (2008).
[PubMed]

Formigaro, L.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Freyer, J. P.

Galieni, P.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Ganser, A.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Georgakoudi, I.

A. Hsiao, M. Hunter, C. Greiner, S. Gupta, and I. Georgakoudi, “Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy,” J. Biomed. Opt. 16(3), 037007 (2011).
[PubMed]

C. Greiner, M. Hunter, P. Huang, F. Rius, and I. Georgakoudi, “Confocal Backscattering Spectroscopy for Leukemic and Normal Blood Cell Discrimination,” Cytometry A 79(10), 866–873 (2011).
[PubMed]

Giacomelli, M.

Gilev, K. V.

Goetze, K.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Greiner, C.

C. Greiner, M. Hunter, P. Huang, F. Rius, and I. Georgakoudi, “Confocal Backscattering Spectroscopy for Leukemic and Normal Blood Cell Discrimination,” Cytometry A 79(10), 866–873 (2011).
[PubMed]

A. Hsiao, M. Hunter, C. Greiner, S. Gupta, and I. Georgakoudi, “Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy,” J. Biomed. Opt. 16(3), 037007 (2011).
[PubMed]

Grimwade, D.

M. C. Béné, D. Grimwade, C. Haferlach, T. Haferlach, G. Zini, and L. European, “Leukemia diagnosis: today and tomorrow,” Eur. J. Haematol. 95(4), 365–373 (2015).
[PubMed]

Grossmann, V.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Guillaud, M.

Gupta, M.

Gupta, S.

A. Hsiao, M. Hunter, C. Greiner, S. Gupta, and I. Georgakoudi, “Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy,” J. Biomed. Opt. 16(3), 037007 (2011).
[PubMed]

Gurjar, R.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Haferlach, C.

M. C. Béné, D. Grimwade, C. Haferlach, T. Haferlach, G. Zini, and L. European, “Leukemia diagnosis: today and tomorrow,” Eur. J. Haematol. 95(4), 365–373 (2015).
[PubMed]

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Haferlach, T.

M. C. Béné, D. Grimwade, C. Haferlach, T. Haferlach, G. Zini, and L. European, “Leukemia diagnosis: today and tomorrow,” Eur. J. Haematol. 95(4), 365–373 (2015).
[PubMed]

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Hagen, N.

D. Watson, N. Hagen, J. Diver, P. Marchand, and M. Chachisvilis, “Elastic light scattering from single cells: orientational dynamics in optical trap,” Biophys. J. 87(2), 1298–1306 (2004).
[PubMed]

Harris, N. L.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Heil, G.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Heinze, B.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Hielscher, A. H.

Hoelzer, D.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Holmes, A. B.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Hsiao, A.

A. Hsiao, M. Hunter, C. Greiner, S. Gupta, and I. Georgakoudi, “Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy,” J. Biomed. Opt. 16(3), 037007 (2011).
[PubMed]

Hu, X. H.

Huang, P.

C. Greiner, M. Hunter, P. Huang, F. Rius, and I. Georgakoudi, “Confocal Backscattering Spectroscopy for Leukemic and Normal Blood Cell Discrimination,” Cytometry A 79(10), 866–873 (2011).
[PubMed]

Hunter, M.

C. Greiner, M. Hunter, P. Huang, F. Rius, and I. Georgakoudi, “Confocal Backscattering Spectroscopy for Leukemic and Normal Blood Cell Discrimination,” Cytometry A 79(10), 866–873 (2011).
[PubMed]

A. Hsiao, M. Hunter, C. Greiner, S. Gupta, and I. Georgakoudi, “Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy,” J. Biomed. Opt. 16(3), 037007 (2011).
[PubMed]

Itzkan, I.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Jaffe, E. S.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Janowska-Wieczorek, A.

Jemal, A.

R. L. Siegel, K. D. Miller, and A. Jemal, “Cancer Statistics, 2017,” CA Cancer J. Clin. 67(1), 7–30 (2017).
[PubMed]

Johnson, T. M.

Kabani, S.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Karmadonova, N. A.

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Kern, W.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Kirchner, H.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Kirkwood, S. E.

Klein, H.-U.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Kline, E.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Kohlmann, A.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Kong, B.

L. Xie, Y. Yang, X. Sun, X. Qiao, Q. Liu, K. Song, B. Kong, and X. Su, “2D light scattering static cytometry for label-free single cell analysis with submicron resolution,” Cytometry A 87(11), 1029–1037 (2015).
[PubMed]

X. Su, S. Liu, X. Qiao, Y. Yang, K. Song, and B. Kong, “Pattern recognition cytometry for label-free cell classification by 2D light scattering measurements,” Opt. Express 23(21), 27558–27565 (2015).
[PubMed]

Konokhova, A. I.

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Krauter, J.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Kreuzer, K.-A.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

LaBauve, P. M.

G. C. Salzman, J. M. Crowell, J. C. Martin, T. T. Trujillo, A. Romero, P. F. Mullaney, and P. M. LaBauve, “Cell classification by laser light scattering: identification and separation of unstained leukocytes,” Acta Cytol. 19(4), 374–377 (1975).
[PubMed]

Lee, J.

Levin, H. S.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Lin, M.

Liso, A.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Lister, T. A.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Litvinenko, A. L.

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Liu, Q.

Liu, S.

Liu, W.

S. An, W. Liu, and S. Venkatesh, “Fast cross-validation algorithms for least squares support vector machine and kernel ridge regression,” Pattern Recognit. 40(8), 2154–2162 (2007).

Loisel, H.

Lu, J. Q.

Lübbert, M.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Macaulay, C.

Maltsev, V. P.

K. V. Gilev, M. A. Yurkin, E. S. Chernyshova, D. I. Strokotov, A. V. Chernyshev, and V. P. Maltsev, “Mature red blood cells: from optical model to inverse light-scattering problem,” Biomed. Opt. Express 7(4), 1305–1310 (2016).
[PubMed]

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Marchand, P.

D. Watson, N. Hagen, J. Diver, P. Marchand, and M. Chachisvilis, “Elastic light scattering from single cells: orientational dynamics in optical trap,” Biophys. J. 87(2), 1298–1306 (2004).
[PubMed]

Marquez-Curtis, L.

Martelli, M. P.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Martin, J. C.

G. C. Salzman, J. M. Crowell, J. C. Martin, T. T. Trujillo, A. Romero, P. F. Mullaney, and P. M. LaBauve, “Cell classification by laser light scattering: identification and separation of unstained leukocytes,” Acta Cytol. 19(4), 374–377 (1975).
[PubMed]

Martinelli, S.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

McGilligan, J. A.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Mériaux, X.

Mestichelli, F.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Miller, K. D.

R. L. Siegel, K. D. Miller, and A. Jemal, “Cancer Statistics, 2017,” CA Cancer J. Clin. 67(1), 7–30 (2017).
[PubMed]

Moran, M. S.

Moskalensky, A. E.

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Mourant, J. R.

Moutier, W.

Mullaney, P. F.

G. C. Salzman, J. M. Crowell, J. C. Martin, T. T. Trujillo, A. Romero, P. F. Mullaney, and P. M. LaBauve, “Cell classification by laser light scattering: identification and separation of unstained leukocytes,” Acta Cytol. 19(4), 374–377 (1975).
[PubMed]

Müller, M. G.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Muller-Hermelink, H. K.

N. L. Harris, E. S. Jaffe, J. Diebold, G. Flandrin, H. K. Muller-Hermelink, J. Vardiman, T. A. Lister, and C. D. Bloomfield, “World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: Report of the Clinical Advisory Committee meeting-Airlie House, Virginia, November 1997,” J. Clin. Oncol. 17(12), 3835–3849 (1999).
[PubMed]

Negrini, M.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Nekrasov, V. M.

A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
[PubMed]

Nichele, I.

G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
[PubMed]

Oruzio, D.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Pascheberg, U.

J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
[PubMed]

Pasqualucci, L.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Peceny, R.

V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
[PubMed]

Perelman, L. T.

V. Backman, M. B. Wallace, L. T. Perelman, J. T. Arendt, R. Gurjar, M. G. Müller, Q. Zhang, G. Zonios, E. Kline, J. A. McGilligan, S. Shapshay, T. Valdez, K. Badizadegan, J. M. Crawford, M. Fitzmaurice, S. Kabani, H. S. Levin, M. Seiler, R. R. Dasari, I. Itzkan, J. Van Dam, and M. S. Feld, “Detection of preinvasive cancer cells,” Nature 406(6791), 35–36 (2000).
[PubMed]

Peter, W.

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A. L. Litvinenko, A. E. Moskalensky, N. A. Karmadonova, V. M. Nekrasov, D. I. Strokotov, A. I. Konokhova, M. A. Yurkin, E. A. Pokushalov, A. V. Chernyshev, and V. P. Maltsev, “Fluorescence-Free Flow Cytometry for Measurement of Shape Index Distribution of Resting, Partially Activated, and Fully Activated Platelets,” Cytometry A 89(11), 1010–1016 (2016).
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J. Krauter, W. Peter, U. Pascheberg, B. Heinze, L. Bergmann, D. Hoelzer, M. Lübbert, G. Schlimok, R. Arnold, H. Kirchner, M. Port, A. Ganser, and G. Heil, “Detection of karyotypic aberrations in acute myeloblastic leukaemia: a prospective comparison between PCR/FISH and standard cytogenetics in 140 patients with de novo AML,” Br. J. Haematol. 103(1), 72–78 (1998).
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Qiu, Y.

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V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
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Rigolin, G. M.

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V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
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V. Grossmann, E. Tiacci, A. B. Holmes, A. Kohlmann, M. P. Martelli, W. Kern, A. Spanhol-Rosseto, H.-U. Klein, M. Dugas, S. Schindela, V. Trifonov, S. Schnittger, C. Haferlach, R. Bassan, V. A. Wells, O. Spinelli, J. Chan, R. Rossi, S. Baldoni, L. De Carolis, K. Goetze, H. Serve, R. Peceny, K.-A. Kreuzer, D. Oruzio, G. Specchia, F. Di Raimondo, F. Fabbiano, M. Sborgia, A. Liso, L. Farinelli, A. Rambaldi, L. Pasqualucci, R. Rabadan, T. Haferlach, and B. Falini, “Whole-exome sequencing identifies somatic mutations of BCOR in acute myeloid leukemia with normal karyotype,” Blood 118(23), 6153–6163 (2011).
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Su, X. T.

Sun, X.

L. Xie, Y. Yang, X. Sun, X. Qiao, Q. Liu, K. Song, B. Kong, and X. Su, “2D light scattering static cytometry for label-free single cell analysis with submicron resolution,” Cytometry A 87(11), 1029–1037 (2015).
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G. M. Rigolin, F. Cibien, S. Martinelli, L. Formigaro, L. Rizzotto, E. Tammiso, E. Saccenti, A. Bardi, F. Cavazzini, M. Ciccone, I. Nichele, G. Pizzolo, F. Zaja, R. Fanin, P. Galieni, A. Dalsass, F. Mestichelli, N. Testa, M. Negrini, and A. Cuneo, “Brief report Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with “normal” FISH: correlations with clinicobiologic parameters,” Blood 119(10), 2310–2313 (2012).
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Figures (5)

Fig. 1
Fig. 1 Schematic of the wide-angle 2D light scattering static cytometer. The device consists of a diode pumped solid state (DPSS) laser as the excitation source, a sandwiched glass chip that holds single suspended microspheres or cells excited by an optical fiber, and a 2D light scattering pattern recording system that composes of a 100 × oil-immersion microscope objective and a CMOS sensor. The acquired experimental 2D patterns are sent to a computer for data analysis. The inset picture shows a cross-section illustration of the scattered light transportation from a scatterer to the objective.
Fig. 2
Fig. 2 Comparisons of wide-angle 2D light scattering patterns between experimental and simulated results of 3.87 μm and 4.19 μm microspheres. (a) and (b) are experimental and simulated 2D patterns from 3.87 μm (mean diameter) microspheres, respectively. (c) and (d) are experimental and simulated 2D patterns from 4.19 μm (mean diameter) microspheres, respectively. The 2D light scattering patterns from the same kind of microspheres (here 3.87 μm or 4.19 μm) are with various fringe distributions in wide-angle light scattering.
Fig. 3
Fig. 3 Comparisons of light scattering intensity distributions between experimental and simulated results of 3.87 μm and 4.19 μm microspheres. (a) shows a representative comparison of the wide-angle light scattering intensity distributions between experimental and simulated results for 3.87 μm microspheres, and (b) shows the comparison for 4.19 μm microspheres.
Fig. 4
Fig. 4 Experimental wide-angle 2D light scattering patterns from different single myeloid leukemic cells. (a), representative 2D patterns of HL-60 cells. (b), representative2D patterns of K562 cells.
Fig. 5
Fig. 5 Scatter plots in the 3D GLDS parameter space of 100 HL-60 cells and 100 K562 cells. The CON, ASM, and ENT texture features were extracted from the 2D patterns of HL-60 cells and K562 cells. Blue and green spherical signs indicate the mean values for these two groups of leukemic cells, and their SD values are illustrated by the blue and green ellipsoids, respectively.

Tables (1)

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Table 1 Classification of HL-60 cells and K562 cells by SVM with LOO-CV

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