Abstract

We propose a compact and easy-to-align lateral shearing common-path digital holographic microscopy, which is based on a slightly trapezoid Sagnac interferometer to create two laterally sheared beams and form off-axis geometry. In this interferometer, the two beams pass through a set of identical optical elements in opposite directions and have nearly the same optical path difference. Without any vibration isolation, the temporal stability of the setup is found to be around 0.011 rad. Compared with highly simple lateral shearing interferometer, the off-axis angle of the setup can be easily adjusted and quantitatively controlled, meanwhile the image quality is not degraded. The experiments for measuring the static and dynamic specimens are performed to demonstrate the capability and applicability.

© 2017 Optical Society of America

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2016 (6)

2015 (3)

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

H. Bai, M. Shan, Z. Zhong, L. Guo, and Y. Zhang, “Common path interferometer based on the modified Michelson configuration using a reflective grating,” Opt. Lasers Eng. 75, 1–4 (2015).
[Crossref]

S. Mahajan, V. Trivedi, P. Vora, V. Chhaniwal, B. Javidi, and A. Anand, “Highly stable digital holographic microscope using Sagnac interferometer,” Opt. Lett. 40(16), 3743–3746 (2015).
[Crossref] [PubMed]

2014 (4)

2013 (1)

2012 (5)

2011 (1)

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

2009 (4)

2008 (2)

B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008).
[Crossref] [PubMed]

R. R. Gattass and E. Mazur, “Femtosecond laser micromachining in transparent materials,” Nat. Photonics 2(4), 219–225 (2008).
[Crossref]

2006 (3)

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett. 31(6), 775–777 (2006).
[Crossref] [PubMed]

Y. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Diffraction phase and fluorescence microscopy,” Opt. Express 14(18), 8263–8268 (2006).
[Crossref] [PubMed]

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

2005 (3)

2002 (1)

1999 (1)

Anand, A.

Arbabi, A.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

Asundi, A.

Badizadegan, K.

Baek, Y.

Bai, H.

H. Bai, M. Shan, Z. Zhong, L. Guo, and Y. Zhang, “Common path interferometer based on the modified Michelson configuration using a reflective grating,” Opt. Lasers Eng. 75, 1–4 (2015).
[Crossref]

Bhaduri, B.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett. 37(6), 1094–1096 (2012).
[Crossref] [PubMed]

Bhattacharya, K.

Bon, P.

Chhaniwal, V.

Chichkov, B. N.

Choi, Y.

Choo, C. O.

Colomb, T.

Cuche, E.

Dai, S.

Dasari, R. R.

Debeir, O.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Decaestecker, C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Depeursinge, C.

Di, J.

Dubois, F.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Edwards, C.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

Eldridge, W. J.

Emery, Y.

Feld, M. S.

Ganti, R.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

Gattass, R. R.

R. R. Gattass and E. Mazur, “Femtosecond laser micromachining in transparent materials,” Nat. Photonics 2(4), 219–225 (2008).
[Crossref]

Girshovitz, P.

Goddard, L. L.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

Guo, L.

H. Bai, M. Shan, Z. Zhong, L. Guo, and Y. Zhang, “Common path interferometer based on the modified Michelson configuration using a reflective grating,” Opt. Lasers Eng. 75, 1–4 (2015).
[Crossref]

Hosseini, P.

Hsu, W.-C.

Ikeda, T.

Javidi, B.

Kang, J. W.

Kemper, B.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008).
[Crossref] [PubMed]

Kim, K.

Kim, M.

Kiss, R.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Lee, K.

Legros, J.-C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Leitgeb, R. A.

Li, E.

Li, P.

Li, Y.

Liu, S.

Lo, C. M.

Ma, C.

Magistretti, P.

Magistretti, P. J.

Mahajan, S.

Mann, C.

Marquet, P.

Maucort, G.

Mazur, E.

R. R. Gattass and E. Mazur, “Femtosecond laser micromachining in transparent materials,” Nat. Photonics 2(4), 219–225 (2008).
[Crossref]

Mir, M.

Monneret, S.

Monnom, O.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Nguyen, T. H.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

Nolte, S.

Park, Y.

Peng, T.

Pham, H.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett. 37(6), 1094–1096 (2012).
[Crossref] [PubMed]

Popescu, G.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

B. Bhaduri, H. Pham, M. Mir, and G. Popescu, “Diffraction phase microscopy with white light,” Opt. Lett. 37(6), 1094–1096 (2012).
[Crossref] [PubMed]

Y. Park, G. Popescu, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Diffraction phase and fluorescence microscopy,” Opt. Express 14(18), 8263–8268 (2006).
[Crossref] [PubMed]

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett. 31(6), 775–777 (2006).
[Crossref] [PubMed]

Qu, W.

Rappaz, B.

Rinehart, M. T.

Roitshtain, D.

Rommel, C. E.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

Schnekenburger, J.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

Shaked, N. T.

Shan, M.

H. Bai, M. Shan, Z. Zhong, L. Guo, and Y. Zhang, “Common path interferometer based on the modified Michelson configuration using a reflective grating,” Opt. Lasers Eng. 75, 1–4 (2015).
[Crossref]

Sheinfeld, A.

Singh, A. S.

So, P. T. C.

Su, J.-W.

Sung, K.-B.

Trivedi, V.

Tseng, T.-Y.

Tünnermann, A.

Turko, N. A.

Van Ham, P.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Vollmer, A.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

von Bally, G.

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

B. Kemper and G. von Bally, “Digital holographic microscopy for live cell applications and technical inspection,” Appl. Opt. 47(4), A52–A61 (2008).
[Crossref] [PubMed]

Vora, P.

Wang, X.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

Wattellier, B.

Wax, A.

Weijuan, Q.

Will, M.

Xi, T.

Xie, M.

Yaqoob, Z.

Yingjie, Y.

Yodh, A. G.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

Yoon, J.

Yourassowsky, C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Yu, L.

Yu, Y.

Yunker, P. J.

C. Edwards, A. Arbabi, B. Bhaduri, X. Wang, R. Ganti, P. J. Yunker, A. G. Yodh, G. Popescu, and L. L. Goddard, “Measuring the non-uniform evaporation dynamics of sprayed sessile microdroplets with quantitative phase imaging,” Langmuir 31(40), 11020–11032 (2015).
[Crossref] [PubMed]

Zhang, J.

Zhang, Y.

H. Bai, M. Shan, Z. Zhong, L. Guo, and Y. Zhang, “Common path interferometer based on the modified Michelson configuration using a reflective grating,” Opt. Lasers Eng. 75, 1–4 (2015).
[Crossref]

Zhao, J.

Zhong, Z.

H. Bai, M. Shan, Z. Zhong, L. Guo, and Y. Zhang, “Common path interferometer based on the modified Michelson configuration using a reflective grating,” Opt. Lasers Eng. 75, 1–4 (2015).
[Crossref]

Zhou, R.

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

Adv. Opt. Photonics (1)

B. Bhaduri, C. Edwards, H. Pham, R. Zhou, T. H. Nguyen, L. L. Goddard, and G. Popescu, “Diffraction phase microscopy: principles and applications in materials and life sciences,” Adv. Opt. Photonics 6(1), 57–119 (2014).
[Crossref]

Appl. Opt. (6)

J. Biomed. Opt. (2)

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

B. Kemper, A. Vollmer, C. E. Rommel, J. Schnekenburger, and G. von Bally, “Simplified approach for quantitative digital holographic phase contrast imaging of living cells,” J. Biomed. Opt. 16(2), 026014 (2011).
[Crossref] [PubMed]

Langmuir (1)

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Supplementary Material (1)

NameDescription
» Visualization 1: MP4 (1312 KB)      3D topography variation

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Figures (7)

Fig. 1
Fig. 1

Experimental setup of the lateral shearing common-path DHM based on a slightly trapezoid Sagnac interferometer; BE: beam expander; L1, L2: lenses; P1, P2: polarizers; M1-M5: mirrors; MO: microscope objective; PBS: polarized beam splitter.

Fig. 2
Fig. 2

Schematic of light beams in the trapezoid Sagnac interferometer. (a) p-polarized component; (b) s-polarized component.

Fig. 3
Fig. 3

Experiment results for demonstration that the off-axis angle of the setup is adjustable by slightly rotating the PBS. (a1)-(a3) Digital holograms recoded at different off-axis angles; (b1)-(b3) Spatial spectra corresponding in 3(a1)-3(a3); (c1)-(c3) Reconstructed Intensity images corresponding in 3(a1)-3(a3).

Fig. 4
Fig. 4

Measurement results of the two pits ablated by high power laser irradiation on the silica glass surface. (a), (d) Phase images of the first and second pits; (b), (e) 3D topographies of the first and second pits; (c), (f) 1D depth profiles along the horizontal line crossing the center of the first and second pits.

Fig. 5
Fig. 5

Measurement results of a tiny deionized water droplet during evaporation process. (a)-(d) Reconstructed phase images at t = 0 s, t = 2 s, t = 4 s, t = 6 s, respectively; (e) 1D thickness profiles along the horizontal line crossing the center of the water droplet corresponding in Figs. 5(a)-5(d). The 3D topography variation during the whole evaporation process can be seen in Visualization 1.

Fig. 6
Fig. 6

Measurement results of a HeLa cell. (a) Quantitative phase image; (b) 3D phase image.

Fig. 7
Fig. 7

Temporal stability of the proposed setup and DHM based on a traditional Mach–Zehnder interferometer. (a) Histogram of standard deviation of the proposed setup; (b) Histogram of standard deviation of the DHM based on a traditional Mach–Zehnder interferometer.

Equations (6)

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θ=2( θ 1 + θ 2 + θ 3 )π.
θ ' =2( θ 1 ' + θ 2 ' + θ 3 ' )π.
θ 1 ' = θ 1 ( θ2 θ 0 ' ),
θ 2 ' = θ 2 +( θ2 θ 0 ' ),
θ 3 ' = θ 3 ( θ2 θ 0 ' ),
Δθ= θ ' θ=4[ ( θ 0 ' + π 2 θ 1 θ 2 θ 3 ) ].

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