Abstract

Pre-chirp technique was used in an Nd-doped fiber amplifier to optimize high-quality 910 nm pulses with the pulses width of 114 fs and pulse energy of 4.4 nJ. The in vivo zebrafish imaging results from our totally home-made microscopy proves our femtosecond Nd fiber laser an ideal source in two-photon microscopic imaging.

© 2016 Optical Society of America

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References

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    [Crossref]

2014 (2)

2013 (1)

2012 (1)

2006 (2)

2005 (2)

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

2003 (1)

J. L. Schwartz, N. A. Bonnet, and G. H. Patterson, “Photobleaching and photoactivation: following protein dynamics in living cells,” Nature Cell Biology 9, 7–14 (2003).

2001 (1)

J. Nakai, M. Ohkura, and K. Imoto, “A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein,” Nat. Biotechnol. 19(2), 137–141 (2001).
[Crossref] [PubMed]

1999 (1)

1996 (2)

C. Xu and W. W. Webb, “Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt.Soc.Am. B 13(3), 481–491 (1996).
[Crossref]

P. D. Dragic and G. C. Papen, “Efficient Amplification Using the 4F3/2 − 4I9/2 Transition in Nd-Doped Silica Fiber,” IEEE Photonics Technol. Lett. 11(12), 1593–1595 (1996).
[Crossref]

1987 (1)

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

1986 (1)

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Ainslie, B. J.

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

Arai, K.

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Barber, D. J.

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

Bonnet, N. A.

J. L. Schwartz, N. A. Bonnet, and G. H. Patterson, “Photobleaching and photoactivation: following protein dynamics in living cells,” Nature Cell Biology 9, 7–14 (2003).

Chang, G.

Chen, B.

Chen, H. W.

Craig, S. P.

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

Davey, S. T.

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

Denk, W.

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

Dragic, P. D.

P. D. Dragic and G. C. Papen, “Efficient Amplification Using the 4F3/2 − 4I9/2 Transition in Nd-Doped Silica Fiber,” IEEE Photonics Technol. Lett. 11(12), 1593–1595 (1996).
[Crossref]

Fittinghoff, D. N.

Gao, X.

George, A. K.

Gomes, A. S. L.

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

Haider, Z.

Handa, T.

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Helmchen, F.

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

Hideur, A.

Honda, T.

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Huang, S. W.

Hui, R.

Imoto, K.

J. Nakai, M. Ohkura, and K. Imoto, “A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein,” Nat. Biotechnol. 19(2), 137–141 (2001).
[Crossref] [PubMed]

Ishii, Y.

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Johnson, C. K.

Jung, Y.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Kärtner, F. X.

Kim, J.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Knight, J. C.

Kumata, K.

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Laroche, M.

Lee, J. W.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Li, C.

Lim, J.

Liu, Y.

Millard, A. C.

Molla, R. G.

Müller, M.

Nakai, J.

J. Nakai, M. Ohkura, and K. Imoto, “A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein,” Nat. Biotechnol. 19(2), 137–141 (2001).
[Crossref] [PubMed]

Namikawa, H.

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

Nilsson, J.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Oh, K.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Ohkura, M.

J. Nakai, M. Ohkura, and K. Imoto, “A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein,” Nat. Biotechnol. 19(2), 137–141 (2001).
[Crossref] [PubMed]

Papen, G. C.

P. D. Dragic and G. C. Papen, “Efficient Amplification Using the 4F3/2 − 4I9/2 Transition in Nd-Doped Silica Fiber,” IEEE Photonics Technol. Lett. 11(12), 1593–1595 (1996).
[Crossref]

Patterson, G. H.

J. L. Schwartz, N. A. Bonnet, and G. H. Patterson, “Photobleaching and photoactivation: following protein dynamics in living cells,” Nature Cell Biology 9, 7–14 (2003).

Price, E. S.

Qian, K.

Sahu, J. K.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Schimpf, D. N.

Schwartz, J. L.

J. L. Schwartz, N. A. Bonnet, and G. H. Patterson, “Photobleaching and photoactivation: following protein dynamics in living cells,” Nature Cell Biology 9, 7–14 (2003).

Soh, D. B. S.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Song, Y.

Squier, J. A.

Stehno-Bittel, L.

Taylor, J. R.

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

Unruh, J. R.

Wang, A.

Wang, H.

Webb, W. W.

C. Xu and W. W. Webb, “Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt.Soc.Am. B 13(3), 481–491 (1996).
[Crossref]

Wilson, K. R.

Wiseman, P. W.

Xu, C.

C. Xu and W. W. Webb, “Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt.Soc.Am. B 13(3), 481–491 (1996).
[Crossref]

Xu, S.

Yang, Z.

Yoo, S.

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Zhang, J.

Zhang, Z.

Zong, W.

Appl. Opt. (1)

IEEE Photonics Technol. Lett. (1)

P. D. Dragic and G. C. Papen, “Efficient Amplification Using the 4F3/2 − 4I9/2 Transition in Nd-Doped Silica Fiber,” IEEE Photonics Technol. Lett. 11(12), 1593–1595 (1996).
[Crossref]

J. Appl. Phys. (1)

K. Arai, H. Namikawa, K. Kumata, T. Honda, Y. Ishii, and T. Handa, “Aluminum or phosphorus codoping effects on the fluorescence and structural properties of neodymium doped silica glass,” J. Appl. Phys. 59(10), 3430–3436 (1986).
[Crossref]

J. Mater. Sci. Lett. (1)

B. J. Ainslie, S. P. Craig, S. T. Davey, D. J. Barber, J. R. Taylor, and A. S. L. Gomes, “Optical and structural investigation of Nd3+ in silica-based fibres,” J. Mater. Sci. Lett. 6(11), 1361–1363 (1987).
[Crossref]

J. Opt.Soc.Am. B (1)

C. Xu and W. W. Webb, “Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm,” J. Opt.Soc.Am. B 13(3), 481–491 (1996).
[Crossref]

Nat. Biotechnol. (1)

J. Nakai, M. Ohkura, and K. Imoto, “A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein,” Nat. Biotechnol. 19(2), 137–141 (2001).
[Crossref] [PubMed]

Nat. Methods (1)

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref] [PubMed]

Nature Cell Biology (1)

J. L. Schwartz, N. A. Bonnet, and G. H. Patterson, “Photobleaching and photoactivation: following protein dynamics in living cells,” Nature Cell Biology 9, 7–14 (2003).

Opt. Commun. (1)

S. Yoo, D. B. S. Soh, J. Kim, Y. Jung, J. Nilsson, J. K. Sahu, J. W. Lee, and K. Oh, “Analysis of W-type waveguide for Nd-doped fiber laser operating near 940 nm,” Opt. Commun. 247(1-3), 153–162 (2005).
[Crossref]

Opt. Express (2)

Opt. Lett. (4)

Other (2)

B. Chen, T. Jiang, Z. Zhang, and A. Wang, “4.4 nJ, 114 fs Nd-doped fiber laser pulses at 920nm for in vivo two-photon microscopic imaging”, Conference on Laser and Electro-Optics (CLEO,2015), paper AM1J.4.
[Crossref]

M. Lang, “Multi-line fibre laser for multi-photon microscopy,” http://www.toptica.com/fileadmin/_migrated/content_uploads/toptica_lang_Photonik_International.pdf .

Supplementary Material (1)

NameDescription
» Visualization 1: AVI (15583 KB)      the blood vessel of a two-day-old zebrafish

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Figures (5)

Fig. 1
Fig. 1 Schematic of the Nd-doped fiber amplifier and the two-photon microscope. WDM, wavelength-division multiplexer; LD, laser diode; L, lens; SC, scanning system; SL, scan lens; TL, tube lens; DM, dichroic mirror; PMT, Photomultiplier tube.
Fig. 2
Fig. 2 The output pulse spectrum with the different pre-chirped GDD.
Fig. 3
Fig. 3 Pulse duration of compressed pulses.
Fig. 4
Fig. 4 Slope efficiency of Nd-doped fiber amplifier.
Fig. 5
Fig. 5 The blood vessel of an flk: GFP zebrafish (see Visualization 1).

Equations (1)

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F(t) 1 2 ϕ η 2 Cδ g p fτ 8n P(t) 2 πλ .

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