Abstract

Due to its sectioning capability, large field of view, and minimal light exposure, selective plane illumination microscopy has become the preferred choice for 3D time lapse imaging. Single cells in a dish can be conveniently imaged using an upright/inverted configuration. However, for measurements on long time scales (hours to days), mechanical drift is a problem; especially for studies of mammalian cells that typically require heating to 37°C which causes a thermal gradient across the instrument. Since the light sheet diverges towards the edges of the field of view, such a drift leads to a decrease in axial resolution over time. Or, even worse, the specimen could move out of the imaging volume. Here, we present a simple, cost-effective way to stabilize the axial position using the microscope camera to track the sample position. Thereby, sample loss is prevented and an optimal axial resolution is maintained by keeping the sample at the position where the light sheet is at its thinnest. We demonstrate the virtue of our approach by measurements of the light sheet thickness and 3D time lapse imaging of a cell monolayer at physiological conditions.

© 2015 Optical Society of America

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  1. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
    [Crossref] [PubMed]
  2. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
    [Crossref] [PubMed]
  3. P. N. Hedde, M. Stakic, and E. Gratton, “Rapid measurement of molecular transport and interaction inside living cells using single plane illumination,” Sci. Rep. 4(7048), 7048 (2014).
    [Crossref] [PubMed]
  4. U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
    [Crossref] [PubMed]
  5. A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
    [Crossref]
  6. N. O. Deakin and C. E. Turner, “Paxillin comes of age,” J. Cell Sci. 121(15), 2435–2444 (2008).
    [Crossref] [PubMed]
  7. M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
    [Crossref] [PubMed]
  8. M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
    [Crossref] [PubMed]
  9. L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
    [Crossref] [PubMed]
  10. E. Baumgart and U. Kubitscheck, “Scanned light sheet microscopy with confocal slit detection,” Opt. Express 20(19), 21805–21814 (2012).
    [Crossref] [PubMed]
  11. T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
    [Crossref] [PubMed]
  12. T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
    [Crossref] [PubMed]
  13. M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
    [Crossref] [PubMed]
  14. L. Gao, “Extend the field of view of selective plan illumination microscopy by tiling the excitation light sheet,” Opt. Express 23(5), 6102–6111 (2015).
    [Crossref] [PubMed]

2015 (1)

2014 (3)

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

P. N. Hedde, M. Stakic, and E. Gratton, “Rapid measurement of molecular transport and interaction inside living cells using single plane illumination,” Sci. Rep. 4(7048), 7048 (2014).
[Crossref] [PubMed]

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

2012 (2)

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
[Crossref] [PubMed]

E. Baumgart and U. Kubitscheck, “Scanned light sheet microscopy with confocal slit detection,” Opt. Express 20(19), 21805–21814 (2012).
[Crossref] [PubMed]

2011 (4)

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref] [PubMed]

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

2009 (1)

M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
[Crossref] [PubMed]

2008 (2)

M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
[Crossref] [PubMed]

N. O. Deakin and C. E. Turner, “Paxillin comes of age,” J. Cell Sci. 121(15), 2435–2444 (2008).
[Crossref] [PubMed]

2004 (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Amodaj, N.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

Baumgart, E.

Betzig, E.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Cižmár, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Coll-Lladó, C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Dalal, R.

M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
[Crossref] [PubMed]

Dalgarno, H. I. C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Davidson, M. W.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Deakin, N. O.

N. O. Deakin and C. E. Turner, “Paxillin comes of age,” J. Cell Sci. 121(15), 2435–2444 (2008).
[Crossref] [PubMed]

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Dholakia, K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Digman, M. A.

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
[Crossref] [PubMed]

M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
[Crossref] [PubMed]

Edelstein, A. D.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

Ermolayev, V.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref] [PubMed]

Ferrier, D. E. K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Friedrich, M.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref] [PubMed]

Fwu, P.

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

Galbraith, C. G.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Galbraith, J. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Gan, Q.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref] [PubMed]

Gao, L.

L. Gao, “Extend the field of view of selective plan illumination microscopy by tiling the excitation light sheet,” Opt. Express 23(5), 6102–6111 (2015).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Giral, H.

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

Gratton, E.

P. N. Hedde, M. Stakic, and E. Gratton, “Rapid measurement of molecular transport and interaction inside living cells using single plane illumination,” Sci. Rep. 4(7048), 7048 (2014).
[Crossref] [PubMed]

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
[Crossref] [PubMed]

M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
[Crossref] [PubMed]

Gunn-Moore, F. J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Gunther, S.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
[Crossref] [PubMed]

Harms, G. S.

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref] [PubMed]

Hedde, P. N.

P. N. Hedde, M. Stakic, and E. Gratton, “Rapid measurement of molecular transport and interaction inside living cells using single plane illumination,” Sci. Rep. 4(7048), 7048 (2014).
[Crossref] [PubMed]

Horwitz, A. F.

M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
[Crossref] [PubMed]

Horwitz, A. R.

M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
[Crossref] [PubMed]

Hufnagel, L.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
[Crossref] [PubMed]

Huisken, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Krzic, U.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
[Crossref] [PubMed]

Kubitscheck, U.

Lanzano, L.

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

Levi, M.

L. Lanzano, M. A. Digman, P. Fwu, H. Giral, M. Levi, and E. Gratton, “Nanometer-scale imaging by the modulation tracking method,” J. Biophotonics 4(6), 415–424 (2011).
[Crossref] [PubMed]

Milkie, D. E.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Nylk, J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Pinkard, H.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

Planchon, T. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8(5), 417–423 (2011).
[Crossref] [PubMed]

Saunders, T. E.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
[Crossref] [PubMed]

Stakic, M.

P. N. Hedde, M. Stakic, and E. Gratton, “Rapid measurement of molecular transport and interaction inside living cells using single plane illumination,” Sci. Rep. 4(7048), 7048 (2014).
[Crossref] [PubMed]

Stelzer, E. H. K.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Streichan, S. J.

U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, “Multiview light-sheet microscope for rapid in toto imaging,” Nat. Methods 9(7), 730–733 (2012).
[Crossref] [PubMed]

Stuurman, N.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

Swoger, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Tsuchida, M. A.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

Turner, C. E.

N. O. Deakin and C. E. Turner, “Paxillin comes of age,” J. Cell Sci. 121(15), 2435–2444 (2008).
[Crossref] [PubMed]

Vale, R. D.

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

Vettenburg, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11(5), 541–544 (2014).
[Crossref] [PubMed]

Wiseman, P. W.

M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
[Crossref] [PubMed]

Wittbrodt, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Biophys. J. (3)

M. A. Digman, P. W. Wiseman, A. R. Horwitz, and E. Gratton, “Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method,” Biophys. J. 96(2), 707–716 (2009).
[Crossref] [PubMed]

M. A. Digman, R. Dalal, A. F. Horwitz, and E. Gratton, “Mapping the number of molecules and brightness in the laser scanning microscope,” Biophys. J. 94(6), 2320–2332 (2008).
[Crossref] [PubMed]

M. Friedrich, Q. Gan, V. Ermolayev, and G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).
[Crossref] [PubMed]

J. Biol. Methods (1)

A. D. Edelstein, M. A. Tsuchida, N. Amodaj, H. Pinkard, R. D. Vale, and N. Stuurman, “Advanced methods of microscope control using μManager software,” J. Biol. Methods 1(2), 10 (2014).
[Crossref]

J. Biophotonics (1)

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Figures (5)

Fig. 1
Fig. 1

Schematic of the active focus stabilization with camera-based detection. The light sheet is reflected off the bottom of the sample container and imaged onto the camera chip. From the position of the sheet on the image the z-position of the sample is calculated. Any displacement is corrected by moving the z-stage.

Fig. 2
Fig. 2

Schematic of the upright/inverted SPIM setup. See main text for description.

Fig. 3
Fig. 3

Flowchart of the 3D time lapse acquisition procedure including focus stabilization. See main text for description.

Fig. 4
Fig. 4

(a) Measurements of the z-position of the sample without (solid symbols) and with active focus stabilization (open symbols) at 25°C (circular symbols) and 37°C (star symbols) over a period of 18 h. (b) Corresponding thickness (1/e2 value) of the light sheet.

Fig. 5
Fig. 5

3D reconstruction of paxillin-EGFP expressing CHO-K1 cells at the indicated time points with (a,b) and without focus stabilization (c,d), both measured at 37°C. The axial displacement due to sample drift, Δz, is indicated in panel (d). Field of view, 178 × 252 × 32 µm3 (xyz). The images were reconstructed using ImageJ 3D Viewer, voxels were painted with 50% transparency.

Equations (2)

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s z = s j a 2 M ,
I( s )= I offset + I 0 exp( 2 ( s j s 0 ) 2 w 2 ),

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