Abstract

Compounds tested during drug development may have adverse effects on the heart; therefore all new chemical entities have to undergo extensive preclinical assessment for cardiac liability. Conventional intensity-based imaging techniques are not robust enough to provide detailed information for cell structure and the captured images result in low-contrast, especially to cell with semi-transparent or transparent feature, which would affect the cell analysis. In this paper we show, for the first time, that digital holographic microscopy (DHM) integrated with information processing algorithms automatically provide dynamic quantitative phase profiles of beating cardiomyocytes. We experimentally demonstrate that relevant parameters of cardiomyocytes can be obtained by our automated algorithm based on DHM phase signal analysis and used to characterize the physiological state of resting cardiomyocytes. Our study opens the possibility of automated quantitative analysis of cardiomyocyte dynamics suitable for further drug safety testing and compounds selection as a new paradigm in drug toxicity screens.

© 2015 Optical Society of America

Full Article  |  PDF Article
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References

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2015 (1)

F. Yi, I. Moon, and Y. H. Lee, “Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy,” J. Biomed. Opt. 20(1), 016005 (2015).
[Crossref] [PubMed]

2014 (1)

B. Rappaz, B. Breton, E. Shaffer, and G. Turcatti, “Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening,” Comb. Chem. High Throughput Screen. 17(1), 80–88 (2014).
[Crossref] [PubMed]

2013 (8)

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

A. El Mallahi, C. Minetti, and F. Dubois, “Automated three-dimensional detection and classification of living organisms using digital holographic microscopy with partial spatial coherent source: application to the monitoring of drinking water resources,” Appl. Opt. 52(1), A68–A80 (2013).
[Crossref] [PubMed]

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
[Crossref] [PubMed]

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
[Crossref] [PubMed]

O. Sirenko, E. F. Cromwell, C. Crittenden, J. A. Wignall, F. A. Wright, and I. Rusyn, “Assessment of beating parameters in human induced pluripotent stem cells enables quantitative in vitro screening for cardiotoxicity,” Toxicol. Appl. Pharmacol. 273(3), 500–507 (2013).
[Crossref] [PubMed]

D. Alsteens, H. Trabelsi, P. Soumillion, and Y. F. Dufrêne, “Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria,” Nat. Commun. 4, 2926 (2013).
[Crossref] [PubMed]

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

2012 (3)

S. M. Wilson and A. Bacic, “Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling of carbohydrate and protein epitopes,” Nat. Protoc. 7(9), 1716–1727 (2012).
[Crossref] [PubMed]

I. Moon, B. Javidi, F. Yi, D. Boss, and P. Marquet, “Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells,” Opt. Express 20(9), 10295–10309 (2012).
[Crossref] [PubMed]

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
[Crossref] [PubMed]

2011 (3)

B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
[Crossref] [PubMed]

M. K. Jonsson, Q. D. Wang, and B. Becker, “Impedance-based detection of beating rhythm and proarrhythmic effects of compounds on stem cell-derived cardiomyocytes,” Assay Drug Dev. Technol. 9(6), 589–599 (2011).
[Crossref] [PubMed]

B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
[Crossref] [PubMed]

2010 (4)

S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
[Crossref] [PubMed]

N. T. Shaked, L. L. Satterwhite, N. Bursac, and A. Wax, “Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy,” Biomed. Opt. Express 1(2), 706–719 (2010).
[Crossref] [PubMed]

E. U. Azeloglu and K. D. Costa, “Cross-bridge cycling gives rise to spatiotemporal heterogeneity of dynamic subcellular mechanics in cardiac myocytes probed with atomic force microscopy,” Am. J. Physiol. Heart Circ. Physiol. 298(3), H853–H860 (2010).
[Crossref] [PubMed]

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
[Crossref] [PubMed]

2009 (6)

I. Moon, M. Daneshpanah, B. Javidi, and A. Stern, “Automated three-dimensional identification and tracking of micro/nanobiological organisms by computational holographic microscopy,” Proc. IEEE 97(6), 990–1010 (2009).
[Crossref]

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

C. L. Hsieh, R. Grange, Y. Pu, and D. Psaltis, “Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging,” Opt. Express 17(4), 2880–2891 (2009).
[Crossref] [PubMed]

A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
[Crossref] [PubMed]

V. Salnikov, Y. O. Lukyanenko, W. J. Lederer, and V. Lukyanenko, “Distribution of ryanodine receptors in rat ventricular myocytes,” J. Muscle Res. Cell Motil. 30(3-4), 161–170 (2009).
[Crossref] [PubMed]

2008 (1)

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
[Crossref] [PubMed]

2007 (1)

J. A. Dykens and Y. Will, “The significance of mitochondrial toxicity testing in drug development,” Drug Discov. Today 12(17-18), 777–785 (2007).
[Crossref] [PubMed]

2006 (1)

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

2005 (3)

2003 (2)

D. J. Stephens and V. J. Allan, “Light microscopy techniques for live cell imaging,” Science 300(5616), 82–86 (2003).
[Crossref] [PubMed]

W. Moerner and D. Fromm, “Methods of single-molecule fluorescence spectroscopy and microscopy,” Rev. Sci. Instrum. 74(8), 3597–3619 (2003).
[Crossref]

2002 (1)

D. L. Hickson-Bick, G. C. Sparagna, L. M. Buja, and J. B. McMillin, “Palmitate-induced apoptosis in neonatal cardiomyocytes is not dependent on the generation of ROS,” Am. J. Physiol. Heart Circ. Physiol. 282(2), H656–H664 (2002).
[Crossref] [PubMed]

1953 (1)

R. Barer, “Determination of dry mass, thickness, solid and water concentration in living cells,” Nature 172(4389), 1097–1098 (1953).
[Crossref] [PubMed]

Abassi, Y. A.

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
[Crossref] [PubMed]

B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
[Crossref] [PubMed]

B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
[Crossref] [PubMed]

Alfieri, D.

Allan, V. J.

D. J. Stephens and V. J. Allan, “Light microscopy techniques for live cell imaging,” Science 300(5616), 82–86 (2003).
[Crossref] [PubMed]

Alsteens, D.

D. Alsteens, H. Trabelsi, P. Soumillion, and Y. F. Dufrêne, “Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria,” Nat. Commun. 4, 2926 (2013).
[Crossref] [PubMed]

Anson, B.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
[Crossref] [PubMed]

O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
[Crossref] [PubMed]

Aoyama, N.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
[Crossref] [PubMed]

Azeloglu, E. U.

E. U. Azeloglu and K. D. Costa, “Cross-bridge cycling gives rise to spatiotemporal heterogeneity of dynamic subcellular mechanics in cardiac myocytes probed with atomic force microscopy,” Am. J. Physiol. Heart Circ. Physiol. 298(3), H853–H860 (2010).
[Crossref] [PubMed]

Bacic, A.

S. M. Wilson and A. Bacic, “Preparation of plant cells for transmission electron microscopy to optimize immunogold labeling of carbohydrate and protein epitopes,” Nat. Protoc. 7(9), 1716–1727 (2012).
[Crossref] [PubMed]

Barbul, A.

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
[Crossref] [PubMed]

Barer, R.

R. Barer, “Determination of dry mass, thickness, solid and water concentration in living cells,” Nature 172(4389), 1097–1098 (1953).
[Crossref] [PubMed]

Becker, B.

M. K. Jonsson, Q. D. Wang, and B. Becker, “Impedance-based detection of beating rhythm and proarrhythmic effects of compounds on stem cell-derived cardiomyocytes,” Assay Drug Dev. Technol. 9(6), 589–599 (2011).
[Crossref] [PubMed]

Benke, A.

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
[Crossref] [PubMed]

Bernhardt, I.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
[Crossref] [PubMed]

Bohlen, H.

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
[Crossref] [PubMed]

Boss, D.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

I. Moon, B. Javidi, F. Yi, D. Boss, and P. Marquet, “Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells,” Opt. Express 20(9), 10295–10309 (2012).
[Crossref] [PubMed]

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
[Crossref] [PubMed]

Braam, S. R.

S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
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Breton, B.

B. Rappaz, B. Breton, E. Shaffer, and G. Turcatti, “Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening,” Comb. Chem. High Throughput Screen. 17(1), 80–88 (2014).
[Crossref] [PubMed]

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

Buja, L. M.

D. L. Hickson-Bick, G. C. Sparagna, L. M. Buja, and J. B. McMillin, “Palmitate-induced apoptosis in neonatal cardiomyocytes is not dependent on the generation of ROS,” Am. J. Physiol. Heart Circ. Physiol. 282(2), H656–H664 (2002).
[Crossref] [PubMed]

Bursac, N.

Callamaras, N.

O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
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Cano, E.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Carlson, C.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
[Crossref] [PubMed]

Chambon, M.

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

Chen, Y. W.

O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
[Crossref] [PubMed]

Colomb, T.

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005).
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Coppola, G.

Costa, K. D.

E. U. Azeloglu and K. D. Costa, “Cross-bridge cycling gives rise to spatiotemporal heterogeneity of dynamic subcellular mechanics in cardiac myocytes probed with atomic force microscopy,” Am. J. Physiol. Heart Circ. Physiol. 298(3), H853–H860 (2010).
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Cotte, Y.

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Crittenden, C.

O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
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O. Sirenko, E. F. Cromwell, C. Crittenden, J. A. Wignall, F. A. Wright, and I. Rusyn, “Assessment of beating parameters in human induced pluripotent stem cells enables quantitative in vitro screening for cardiotoxicity,” Toxicol. Appl. Pharmacol. 273(3), 500–507 (2013).
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Cromwell, E. F.

O. Sirenko, E. F. Cromwell, C. Crittenden, J. A. Wignall, F. A. Wright, and I. Rusyn, “Assessment of beating parameters in human induced pluripotent stem cells enables quantitative in vitro screening for cardiotoxicity,” Toxicol. Appl. Pharmacol. 273(3), 500–507 (2013).
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O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
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Cuche, E.

Daneshpanah, M.

I. Moon, M. Daneshpanah, B. Javidi, and A. Stern, “Automated three-dimensional identification and tracking of micro/nanobiological organisms by computational holographic microscopy,” Proc. IEEE 97(6), 990–1010 (2009).
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De Nicola, S.

Debeir, O.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Decaestecker, C.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Depeursinge, C.

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
[Crossref] [PubMed]

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005).
[Crossref] [PubMed]

Dirksen, D.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
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A. El Mallahi, C. Minetti, and F. Dubois, “Automated three-dimensional detection and classification of living organisms using digital holographic microscopy with partial spatial coherent source: application to the monitoring of drinking water resources,” Appl. Opt. 52(1), A68–A80 (2013).
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F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
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D. Alsteens, H. Trabelsi, P. Soumillion, and Y. F. Dufrêne, “Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria,” Nat. Commun. 4, 2926 (2013).
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J. A. Dykens and Y. Will, “The significance of mitochondrial toxicity testing in drug development,” Drug Discov. Today 12(17-18), 777–785 (2007).
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Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
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Einhorn, S.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
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El Mallahi, A.

Emery, Y.

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
[Crossref] [PubMed]

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005).
[Crossref] [PubMed]

Ferraro, P.

Fiene, S.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
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O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
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Georgiev, G.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
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Grange, R.

Grilli, S.

Hesley, J.

O. Sirenko, C. Crittenden, N. Callamaras, J. Hesley, Y. W. Chen, C. Funes, I. Rusyn, B. Anson, and E. F. Cromwell, “Multiparameter in vitro assessment of compound effects on cardiomyocyte physiology using iPSC cells,” J. Biomol. Screen. 18(1), 39–53 (2013).
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Hickson-Bick, D. L.

D. L. Hickson-Bick, G. C. Sparagna, L. M. Buja, and J. B. McMillin, “Palmitate-induced apoptosis in neonatal cardiomyocytes is not dependent on the generation of ROS,” Am. J. Physiol. Heart Circ. Physiol. 282(2), H656–H664 (2002).
[Crossref] [PubMed]

Hsieh, C. L.

Ivanova, L.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
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Javidi, B.

Jonsson, M. K.

M. K. Jonsson, Q. D. Wang, and B. Becker, “Impedance-based detection of beating rhythm and proarrhythmic effects of compounds on stem cell-derived cardiomyocytes,” Assay Drug Dev. Technol. 9(6), 589–599 (2011).
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Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
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Kamgoué, A.

A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
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Kattman, S.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
[Crossref] [PubMed]

Kemper, B.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
[Crossref] [PubMed]

Ketelhut, S.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
[Crossref] [PubMed]

Kettenhofen, R.

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
[Crossref] [PubMed]

Kiss, R.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Kolossov, E.

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
[Crossref] [PubMed]

Koonce, C.

C. Carlson, C. Koonce, N. Aoyama, S. Einhorn, S. Fiene, A. Thompson, B. Swanson, B. Anson, and S. Kattman, “Phenotypic screening with human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy,” J. Biomol. Screen. 18(10), 1203–1211 (2013).
[Crossref] [PubMed]

Korenstein, R.

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
[Crossref] [PubMed]

Kühn, J.

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

Langehanenberg, P.

P. Langehanenberg, L. Ivanova, I. Bernhardt, S. Ketelhut, A. Vollmer, D. Dirksen, G. Georgiev, G. von Bally, and B. Kemper, “Automated three-dimensional tracking of living cells by digital holographic microscopy,” J. Biomed. Opt. 14(1), 014018 (2009).
[Crossref] [PubMed]

Lederer, W. J.

V. Salnikov, Y. O. Lukyanenko, W. J. Lederer, and V. Lukyanenko, “Distribution of ryanodine receptors in rat ventricular myocytes,” J. Muscle Res. Cell Motil. 30(3-4), 161–170 (2009).
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Lee, Y. H.

F. Yi, I. Moon, and Y. H. Lee, “Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy,” J. Biomed. Opt. 20(1), 016005 (2015).
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Legros, J. C.

F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[Crossref] [PubMed]

Li, N.

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
[Crossref] [PubMed]

B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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Lukyanenko, V.

V. Salnikov, Y. O. Lukyanenko, W. J. Lederer, and V. Lukyanenko, “Distribution of ryanodine receptors in rat ventricular myocytes,” J. Muscle Res. Cell Motil. 30(3-4), 161–170 (2009).
[Crossref] [PubMed]

Lukyanenko, Y. O.

V. Salnikov, Y. O. Lukyanenko, W. J. Lederer, and V. Lukyanenko, “Distribution of ryanodine receptors in rat ventricular myocytes,” J. Muscle Res. Cell Motil. 30(3-4), 161–170 (2009).
[Crossref] [PubMed]

Magistretti, P.

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

Magistretti, P. J.

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
[Crossref] [PubMed]

B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
[Crossref] [PubMed]

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005).
[Crossref] [PubMed]

Marquet, P.

D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy,” J. Biomed. Opt. 18(3), 036007 (2013).
[Crossref] [PubMed]

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
[Crossref]

I. Moon, B. Javidi, F. Yi, D. Boss, and P. Marquet, “Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells,” Opt. Express 20(9), 10295–10309 (2012).
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B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
[Crossref] [PubMed]

B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
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P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005).
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D. L. Hickson-Bick, G. C. Sparagna, L. M. Buja, and J. B. McMillin, “Palmitate-induced apoptosis in neonatal cardiomyocytes is not dependent on the generation of ROS,” Am. J. Physiol. Heart Circ. Physiol. 282(2), H656–H664 (2002).
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S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
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F. Yi, I. Moon, and Y. H. Lee, “Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy,” J. Biomed. Opt. 20(1), 016005 (2015).
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I. Moon, B. Javidi, F. Yi, D. Boss, and P. Marquet, “Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells,” Opt. Express 20(9), 10295–10309 (2012).
[Crossref] [PubMed]

I. Moon, M. Daneshpanah, B. Javidi, and A. Stern, “Automated three-dimensional identification and tracking of micro/nanobiological organisms by computational holographic microscopy,” Proc. IEEE 97(6), 990–1010 (2009).
[Crossref]

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N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
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S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
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A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
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S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
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N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
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[Crossref] [PubMed]

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[Crossref] [PubMed]

P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, “Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy,” Opt. Lett. 30(5), 468–470 (2005).
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A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
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B. Rappaz, B. Breton, E. Shaffer, and G. Turcatti, “Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening,” Comb. Chem. High Throughput Screen. 17(1), 80–88 (2014).
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D. L. Hickson-Bick, G. C. Sparagna, L. M. Buja, and J. B. McMillin, “Palmitate-induced apoptosis in neonatal cardiomyocytes is not dependent on the generation of ROS,” Am. J. Physiol. Heart Circ. Physiol. 282(2), H656–H664 (2002).
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S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
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Y. Cotte, F. Toy, P. Jourdain, N. Pavillon, D. Boss, P. Magistretti, P. Marquet, and C. Depeursinge, “Marker-free phase nanoscopy,” Nat. Photonics 7(2), 113–117 (2013).
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D. Alsteens, H. Trabelsi, P. Soumillion, and Y. F. Dufrêne, “Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria,” Nat. Commun. 4, 2926 (2013).
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A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
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B. Rappaz, B. Breton, E. Shaffer, and G. Turcatti, “Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening,” Comb. Chem. High Throughput Screen. 17(1), 80–88 (2014).
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A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
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S. R. Braam, L. Tertoolen, A. van de Stolpe, T. Meyer, R. Passier, and C. L. Mummery, “Prediction of drug-induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes,” Stem Cell Res. (Amst.) 4(2), 107–116 (2010).
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F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
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Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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F. Dubois, C. Yourassowsky, O. Monnom, J. C. Legros, O. Debeir, P. Van Ham, R. Kiss, and C. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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Am. J. Physiol. Heart Circ. Physiol. (2)

D. L. Hickson-Bick, G. C. Sparagna, L. M. Buja, and J. B. McMillin, “Palmitate-induced apoptosis in neonatal cardiomyocytes is not dependent on the generation of ROS,” Am. J. Physiol. Heart Circ. Physiol. 282(2), H656–H664 (2002).
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Appl. Opt. (1)

Assay Drug Dev. Technol. (2)

J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti, “Label-free cytotoxicity screening assay by digital holographic microscopy,” Assay Drug Dev. Technol. 11(2), 101–107 (2013).
[Crossref] [PubMed]

M. K. Jonsson, Q. D. Wang, and B. Becker, “Impedance-based detection of beating rhythm and proarrhythmic effects of compounds on stem cell-derived cardiomyocytes,” Assay Drug Dev. Technol. 9(6), 589–599 (2011).
[Crossref] [PubMed]

Biomed. Opt. Express (1)

Br. J. Pharmacol. (1)

Y. A. Abassi, B. Xi, N. Li, W. Ouyang, A. Seiler, M. Watzele, R. Kettenhofen, H. Bohlen, A. Ehlich, E. Kolossov, X. Wang, and X. Xu, “Dynamic monitoring of beating periodicity of stem cell-derived cardiomyocytes as a predictive tool for preclinical safety assessment,” Br. J. Pharmacol. 165(5), 1424–1441 (2012).
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Comb. Chem. High Throughput Screen. (1)

B. Rappaz, B. Breton, E. Shaffer, and G. Turcatti, “Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening,” Comb. Chem. High Throughput Screen. 17(1), 80–88 (2014).
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Cytometry A (2)

A. Kamgoué, J. Ohayon, Y. Usson, L. Riou, and P. Tracqui, “Quantification of cardiomyocyte contraction based on image correlation analysis,” Cytometry A 75(4), 298–308 (2009).
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B. Rappaz, A. Barbul, Y. Emery, R. Korenstein, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer,” Cytometry A 73(10), 895–903 (2008).
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Drug Discov. Today (1)

J. A. Dykens and Y. Will, “The significance of mitochondrial toxicity testing in drug development,” Drug Discov. Today 12(17-18), 777–785 (2007).
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B. Xi, T. Wang, N. Li, W. Ouyang, W. Zhang, J. Wu, X. Xu, X. Wang, and Y. A. Abassi, “Functional cardiotoxicity profiling and screening using the xCELLigence RTCA Cardio System,” J Lab Autom 16(6), 415–421 (2011).
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J. Biomed. Opt. (5)

F. Yi, I. Moon, and Y. H. Lee, “Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy,” J. Biomed. Opt. 20(1), 016005 (2015).
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B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti, and P. Marquet, “Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy,” J. Biomed. Opt. 14(3), 034049 (2009).
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N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy,” J. Biophotonics 3(7), 432–436 (2010).
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Figures (14)

Fig. 1
Fig. 1 The schematic of the off-axis digital holographic microscopy (DHM).
Fig. 2
Fig. 2 The optical path difference (OPD) [nm] images of cardiomyocytes captured at different times (among total 540 frames). (a) Cardiomyocyte OPD image at the minimum of peaks. (b) Cardiomyocyte OPD image at the maximum of peaks. (c) The difference image between (a) and (b).
Fig. 3
Fig. 3 Threshold cardiomyocyte image (red color denotes background after thresholding).
Fig. 4
Fig. 4 Beating activity of cardiomyocyte (inset shows a single beat).
Fig. 5
Fig. 5 Beating activity of cardiomyocyte under different threshold values.
Fig. 6
Fig. 6 Detected peaks in beating profile of cardiomyocytes. (a) Detected peaks on raw data. (b) Filtered peaks based on results of (a).
Fig. 7
Fig. 7 Illustration of the fitted curves with parameters within one beating period on cardiomyocytes beating profile.
Fig. 8
Fig. 8 Measured beating profile with variance information (inset shows a single beat).
Fig. 9
Fig. 9 Detected peaks on cardiomyocyte beating profile. (a) Detected multiple peaks. (b) Detected multiple peaks with some false peaks removed.
Fig. 10
Fig. 10 Illustration of the difference images. (a) Different image at the minimum of a beat. (b) Different image at the maximum of a beat.
Fig. 11
Fig. 11 Cardiomyocytes beating profile with contraction and relaxation information. (a) Raw data of cardiomyocytes beating profile. (b) Cardiomyocytes beating profile with contraction and relaxation peaks indicated (inset shows a single beat with contraction and relaxation peaks).
Fig. 12
Fig. 12 Detected peaks based on four more cardiomyocyte sequences with the method in the Section 3.1. (a) Cardiomyocyte image sequences acquired with difficult conditions. (b), (c), and (d) “noise-free” recordings (i.e. no debris interfering with the measurement) similar to the previous recording.
Fig. 13
Fig. 13 Detected peaks based on four more cardiomyocyte sequences with the method in the Section 3.2. (a) cardiomyocyte image sequences acquired with difficult conditions. (b), (c), and (d) “noise-free” recordings (i.e. no debris interfering with the measurement) similar to the previous recording.
Fig. 14
Fig. 14 Detected peaks based on four more cardiomyocyte sequences with the method in the Section 3.3. (a) cardiomyocyte image sequences acquired with difficult conditions. (b), (c), and (d) “noise-free” recordings (i.e. no debris interfering with the measurement) similar to the previous recording.

Tables (7)

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Table 1 Characteristic Parameters of Cardiomyocyte.

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Table 2 Measured Values for Multiple Parameters on Cardiomyocytes Beating Profile.

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Table 3 Measured Multiple Parameter on Cardiomyocyte Beating Profile.

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Table 4 Measured Parameters on Cardiomyocyte Contraction and Relaxation Curve.

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Table 5 Measured Values for Multiple Parameters on Cardiomyocytes Beating Profile on five sequences.

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Table 6 Measured Multiple Parameter on Cardiomyocyte Beating Profile on five sequences.

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Table 7 Measured Parameters on Cardiomyocyte Contraction and Relaxation Curve on five sequences.

Equations (4)

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OPD(x,y)=d(x,y)×[ n c (x,y) n m ],
opd ¯ (i) =average(opd_thresh (x,y) (i) ), withopd_thresh (x,y) (i) ={ opd (x,y) (i) ifopd (x,y) (i) max(opd (x,y) (i) )×0.10 0ifopd (x,y) (i) <max(opd (x,y) (i) )×0.10
f(x)= i=1 10 a i x 10i ,
δ opd (i) =variance[ opd (x,y) (i) opd ¯ temp ],

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