Abstract

We present a method to increase the number of simultaneously imaged focal planes in diffractive multi-plane imaging. We exploit the chromatic properties of diffraction by using multicolor LED illumination and demonstrate time-synchronous imaging of up to 21 focal planes.We discuss the possibilities and limits given by the use of a liquid crystal spatial light modulator to display the diffractive patterns. The method is suitable for wide-field transmission and reflection microscopy.

© 2013 osa

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2013 (2)

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

P. S. Salter, Z. Iqbal, and M. J. Booth, “Analysis of the three-dimensional focal positioning capability of adaptive optic elements,” Int. J. Optomechatronics7, 1–14 (2013).
[CrossRef]

2012 (3)

2011 (2)

M. D. Lew, S. F. Lee, M. Badieirostami, and W. E. Moerner, “Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects,” Opt. Lett.36, 202–204 (2011).
[CrossRef] [PubMed]

Y. Luo, I. K. Zervantonakis, S. B. Oh, R. D. Kamm, and G. Barbastathis, “Spectrally resolved multidepth fluorescence imaging,” J. Biomed. Opt.16, 096015 (2011).
[CrossRef] [PubMed]

2010 (2)

2009 (2)

L. Golan, I. Reutsky, N. Farah, and S. Shoham, “Design and characteristics of holographic neural photo-stimulation systems,” J Neural Eng.6, 066004 (2009).
[CrossRef] [PubMed]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

2008 (2)

Y. Luo, P. J. Gelsinger-Austin, J. M. Watson, G. Barbastathis, J. K. Barton, and R. K. Kostuk, “Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system,” Opt. Lett.33, 2098–2100 (2008).
[CrossRef] [PubMed]

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

2007 (6)

T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Bioph. Res. Co.359, 1–7 (2007).
[CrossRef]

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “Aberration-free optical refocusing in high numerical aperture microscopy,” Opt. Lett.32, 2007–2009 (2007).
[CrossRef] [PubMed]

E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7, 2043–2045 (2007).
[CrossRef] [PubMed]

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett.90, 053902 (2007).
[CrossRef]

R. Di Leonardo, F. Ianni, and G. Ruocco, “Computer generation of optimal holograms for optical trap arrays,” Opt. Express15, 1913–1922 (2007).
[CrossRef] [PubMed]

D. Palima and V. R. Daria, “Holographic projection of arbitrary light patterns with a suppressed zero-order beam,” Appl. Opt.46, 4197–4201 (2007).
[CrossRef] [PubMed]

2006 (4)

J. Sheng, E. Malkiel, and J. Katz, “Digital holographic microscope for measuring three-dimensional particle distributions and motions,” Appl. Opt.45, 3893–3901 (2006).
[CrossRef] [PubMed]

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91, 4258–4272 (2006).
[CrossRef] [PubMed]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Meth.3, 793–795 (2006).
[CrossRef]

2005 (3)

2004 (1)

P. Prabhat, S. Ram, E. Ward, and R. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobiosci.3, 237–242 (2004).
[CrossRef]

2003 (1)

2000 (1)

P. Blanchard and A. Greenaway, “Broadband simultaneous multiplane imaging,” Opt. Commun.183, 29–36 (2000).
[CrossRef]

1999 (1)

1993 (1)

1955 (1)

Abrahamsson, S.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Agard, D. A.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Alfieri, D.

Badieirostami, M.

Balci, H.

E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7, 2043–2045 (2007).
[CrossRef] [PubMed]

Barbastathis, G.

Bargmann, C. I.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Barton, J. K.

Bates, M.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Meth.3, 793–795 (2006).
[CrossRef]

Bennett, B. T.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Bernet, S.

Betzig, E.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Bewersdorf, J.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Biteen, J. S.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Blanchard, P.

P. Blanchard and A. Greenaway, “Broadband simultaneous multiplane imaging,” Opt. Commun.183, 29–36 (2000).
[CrossRef]

P. Blanchard and A. Greenaway, “Simultaneous multiplane imaging with a distorted diffraction grating,” Appl. Opt.38, 6692–6699 (1999).
[CrossRef]

Blehm, B. H.

E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7, 2043–2045 (2007).
[CrossRef] [PubMed]

Bonifacino, J. S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Booth, M. J.

Botcherby, E. J.

Brooker, G.

J. Rosen and G. Brooker, “Fresnel incoherent correlation holography (FINCH): a review of research,” Adv. Opt. Tech.1, 151–169 (2012).

Chen, J.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Colomb, T.

Coppola, G.

Cuche, E.

Dahan, M.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Dalgarno, P. A.

Daria, V. R.

Darzacq, C. D.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Darzacq, X.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Davidson, M. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

De Nicola, S.

de Sars, V.

Depeursinge, C.

Di Francia, G.

Di Leonardo, R.

Emery, Y.

Emiliani, V.

Farah, N.

L. Golan, I. Reutsky, N. Farah, and S. Shoham, “Design and characteristics of holographic neural photo-stimulation systems,” J Neural Eng.6, 066004 (2009).
[CrossRef] [PubMed]

Fassl, S.

Feng, Y.

Ferraro, P.

Finizio, A.

Gelsinger-Austin, P. J.

Girirajan, T. P. K.

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91, 4258–4272 (2006).
[CrossRef] [PubMed]

Golan, L.

L. Golan, I. Reutsky, N. Farah, and S. Shoham, “Design and characteristics of holographic neural photo-stimulation systems,” J Neural Eng.6, 066004 (2009).
[CrossRef] [PubMed]

Gonda, K.

T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Bioph. Res. Co.359, 1–7 (2007).
[CrossRef]

Gould, T. J.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Greenaway, A.

P. Blanchard and A. Greenaway, “Broadband simultaneous multiplane imaging,” Opt. Commun.183, 29–36 (2000).
[CrossRef]

P. Blanchard and A. Greenaway, “Simultaneous multiplane imaging with a distorted diffraction grating,” Appl. Opt.38, 6692–6699 (1999).
[CrossRef]

Greenaway, A. H.

Grilli, S.

Guillon, M.

Gustafsson, M. G. L.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Hajj, B.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Hess, H. F.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Hess, S. T.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91, 4258–4272 (2006).
[CrossRef] [PubMed]

Higuchi, H.

T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Bioph. Res. Co.359, 1–7 (2007).
[CrossRef]

Holtzer, L.

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett.90, 053902 (2007).
[CrossRef]

Ianni, F.

Iqbal, Z.

P. S. Salter, Z. Iqbal, and M. J. Booth, “Analysis of the three-dimensional focal positioning capability of adaptive optic elements,” Int. J. Optomechatronics7, 1–14 (2013).
[CrossRef]

Javidi, B.

Jericho, M.

Jesacher, A.

A. Jesacher and M. J. Booth, “Parallel direct laser writing in three dimensions with spatially dependent aberration correction,” Opt. Express18, 21090–21099 (2010).
[CrossRef] [PubMed]

A. Jesacher and M. Ritsch-Marte, “Multi-focal light microscopy using liquid crystal spatial light modulators,” in “International Symposium on Optomechatronic Technologies (ISOT) 2012,” (2012), pp. 1–2.
[CrossRef]

Juette, M. F.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Juskaitis, R.

Kamm, R. D.

Y. Luo, I. K. Zervantonakis, S. B. Oh, R. D. Kamm, and G. Barbastathis, “Spectrally resolved multidepth fluorescence imaging,” J. Biomed. Opt.16, 096015 (2011).
[CrossRef] [PubMed]

Katsov, A. Y.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Katz, J.

Khan, S.

Kostuk, R. K.

Koumoutsakos, P.

I. Sbalzarini and P. Koumoutsakos, “Feature point tracking and trajectory analysis for video imaging in cell biology,” J. Struct. Biol.151, 182–195 (2005).
[CrossRef] [PubMed]

Kreuzer, H.

Lee, D.

Lee, S. F.

Lessard, M. D.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Lew, M. D.

Lindwasser, O. W.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Lippincott-Schwartz, J.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Liu, N.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Lord, S. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Love, G.

Luo, Y.

Magistretti, P.

Malkiel, E.

Marquet, P.

Mason, M. D.

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91, 4258–4272 (2006).
[CrossRef] [PubMed]

Maurer, C.

Meckel, T.

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett.90, 053902 (2007).
[CrossRef]

Meinertzhagen, I.

Mizuguchi, G.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Mlodzianoski, M. J.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Moerner, W. E.

M. D. Lew, S. F. Lee, M. Badieirostami, and W. E. Moerner, “Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects,” Opt. Lett.36, 202–204 (2011).
[CrossRef] [PubMed]

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Mueller, F.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Nagpure, B. S.

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

Ober, R.

P. Prabhat, S. Ram, E. Ward, and R. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobiosci.3, 237–242 (2004).
[CrossRef]

Oh, S. B.

Y. Luo, I. K. Zervantonakis, S. B. Oh, R. D. Kamm, and G. Barbastathis, “Spectrally resolved multidepth fluorescence imaging,” J. Biomed. Opt.16, 096015 (2011).
[CrossRef] [PubMed]

Olenych, S.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Palima, D.

Patterson, G. H.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Pavani, S. R. P.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Pierattini, G.

Piestun, R.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Prabhat, P.

P. Prabhat, S. Ram, E. Ward, and R. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobiosci.3, 237–242 (2004).
[CrossRef]

Ram, S.

P. Prabhat, S. Ram, E. Ward, and R. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobiosci.3, 237–242 (2004).
[CrossRef]

Rappaz, B.

Reutsky, I.

L. Golan, I. Reutsky, N. Farah, and S. Shoham, “Design and characteristics of holographic neural photo-stimulation systems,” J Neural Eng.6, 066004 (2009).
[CrossRef] [PubMed]

Ritsch-Marte, M.

C. Maurer, S. Khan, S. Fassl, S. Bernet, and M. Ritsch-Marte, “Depth of field multiplexing in microscopy,” Opt. Express18, 3023–3034 (2010).
[CrossRef] [PubMed]

A. Jesacher and M. Ritsch-Marte, “Multi-focal light microscopy using liquid crystal spatial light modulators,” in “International Symposium on Optomechatronic Technologies (ISOT) 2012,” (2012), pp. 1–2.
[CrossRef]

Ronzitti, E.

Rosen, J.

J. Rosen and G. Brooker, “Fresnel incoherent correlation holography (FINCH): a review of research,” Adv. Opt. Tech.1, 151–169 (2012).

Ruocco, G.

Rust, M. J.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Meth.3, 793–795 (2006).
[CrossRef]

Salter, P. S.

P. S. Salter, Z. Iqbal, and M. J. Booth, “Analysis of the three-dimensional focal positioning capability of adaptive optic elements,” Int. J. Optomechatronics7, 1–14 (2013).
[CrossRef]

Sato, T.

T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Bioph. Res. Co.359, 1–7 (2007).
[CrossRef]

Sbalzarini, I.

I. Sbalzarini and P. Koumoutsakos, “Feature point tracking and trajectory analysis for video imaging in cell biology,” J. Struct. Biol.151, 182–195 (2005).
[CrossRef] [PubMed]

Schmidt, T.

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett.90, 053902 (2007).
[CrossRef]

Selvin, P. R.

E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7, 2043–2045 (2007).
[CrossRef] [PubMed]

Sheng, J.

Shoham, S.

L. Golan, I. Reutsky, N. Farah, and S. Shoham, “Design and characteristics of holographic neural photo-stimulation systems,” J Neural Eng.6, 066004 (2009).
[CrossRef] [PubMed]

Sougrat, R.

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Soule, P.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Stallinga, S.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Striano, V.

Thompson, M. A.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Thomson, R. R.

Toprak, E.

E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7, 2043–2045 (2007).
[CrossRef] [PubMed]

Twieg, R. J.

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Ward, E.

P. Prabhat, S. Ram, E. Ward, and R. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobiosci.3, 237–242 (2004).
[CrossRef]

Watanabe, T. M.

T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Bioph. Res. Co.359, 1–7 (2007).
[CrossRef]

Watson, J. M.

Wilson, T.

Wisniewski, J.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Wu, C.

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

Xu, W.

Yang, Y.

Zervantonakis, I. K.

Y. Luo, I. K. Zervantonakis, S. B. Oh, R. D. Kamm, and G. Barbastathis, “Spectrally resolved multidepth fluorescence imaging,” J. Biomed. Opt.16, 096015 (2011).
[CrossRef] [PubMed]

Zhuang, X.

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Meth.3, 793–795 (2006).
[CrossRef]

Adv. Opt. Tech. (1)

J. Rosen and G. Brooker, “Fresnel incoherent correlation holography (FINCH): a review of research,” Adv. Opt. Tech.1, 151–169 (2012).

Appl. Opt. (4)

Appl. Phys. Lett. (1)

L. Holtzer, T. Meckel, and T. Schmidt, “Nanometric three-dimensional tracking of individual quantum dots in cells,” Appl. Phys. Lett.90, 053902 (2007).
[CrossRef]

Biochem. Bioph. Res. Co. (1)

T. M. Watanabe, T. Sato, K. Gonda, and H. Higuchi, “Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics,” Biochem. Bioph. Res. Co.359, 1–7 (2007).
[CrossRef]

Biophys. J. (1)

S. T. Hess, T. P. K. Girirajan, and M. D. Mason, “Ultra-high resolution imaging by fluorescence photoactivation localization microscopy,” Biophys. J.91, 4258–4272 (2006).
[CrossRef] [PubMed]

IEEE Trans. Nanobiosci. (1)

P. Prabhat, S. Ram, E. Ward, and R. Ober, “Simultaneous imaging of different focal planes in fluorescence microscopy for the study of cellular dynamics in three dimensions,” IEEE Trans. Nanobiosci.3, 237–242 (2004).
[CrossRef]

Int. J. Optomechatronics (1)

P. S. Salter, Z. Iqbal, and M. J. Booth, “Analysis of the three-dimensional focal positioning capability of adaptive optic elements,” Int. J. Optomechatronics7, 1–14 (2013).
[CrossRef]

J Neural Eng. (1)

L. Golan, I. Reutsky, N. Farah, and S. Shoham, “Design and characteristics of holographic neural photo-stimulation systems,” J Neural Eng.6, 066004 (2009).
[CrossRef] [PubMed]

J. Biomed. Opt. (1)

Y. Luo, I. K. Zervantonakis, S. B. Oh, R. D. Kamm, and G. Barbastathis, “Spectrally resolved multidepth fluorescence imaging,” J. Biomed. Opt.16, 096015 (2011).
[CrossRef] [PubMed]

J. Opt. Soc. Am. (1)

J. Struct. Biol. (1)

I. Sbalzarini and P. Koumoutsakos, “Feature point tracking and trajectory analysis for video imaging in cell biology,” J. Struct. Biol.151, 182–195 (2005).
[CrossRef] [PubMed]

Nano Lett. (1)

E. Toprak, H. Balci, B. H. Blehm, and P. R. Selvin, “Three-dimensional particle tracking via bifocal imaging,” Nano Lett.7, 2043–2045 (2007).
[CrossRef] [PubMed]

Nat. Meth. (3)

S. Abrahamsson, J. Chen, B. Hajj, S. Stallinga, A. Y. Katsov, J. Wisniewski, G. Mizuguchi, P. Soule, F. Mueller, C. D. Darzacq, X. Darzacq, C. Wu, C. I. Bargmann, D. A. Agard, M. Dahan, and M. G. L. Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Meth.10, 60–63 (2013).
[CrossRef]

M. F. Juette, T. J. Gould, M. D. Lessard, M. J. Mlodzianoski, B. S. Nagpure, B. T. Bennett, S. T. Hess, and J. Bewersdorf, “Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples,” Nat. Meth.5, 527–529 (2008).
[CrossRef]

M. J. Rust, M. Bates, and X. Zhuang, “Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM),” Nat. Meth.3, 793–795 (2006).
[CrossRef]

Opt. Commun. (1)

P. Blanchard and A. Greenaway, “Broadband simultaneous multiplane imaging,” Opt. Commun.183, 29–36 (2000).
[CrossRef]

Opt. Express (6)

Opt. Lett. (5)

Proc. Natl. Acad. Sci. USA (1)

S. R. P. Pavani, M. A. Thompson, J. S. Biteen, S. J. Lord, N. Liu, R. J. Twieg, R. Piestun, and W. E. Moerner, “Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function,” Proc. Natl. Acad. Sci. USA106, 2995–2999 (2009).
[CrossRef] [PubMed]

Science (1)

E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess, “Imaging intracellular fluorescent proteins at nanometer resolution,” Science313, 1642–1645 (2006).
[CrossRef] [PubMed]

Other (1)

A. Jesacher and M. Ritsch-Marte, “Multi-focal light microscopy using liquid crystal spatial light modulators,” in “International Symposium on Optomechatronic Technologies (ISOT) 2012,” (2012), pp. 1–2.
[CrossRef]

Supplementary Material (1)

» Media 1: AVI (250 KB)     

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Figures (5)

Fig. 1
Fig. 1

Left: experimental set-up; chromatic dependence of diffraction leads to color-dependent focus values and thus to an increased number of planes that can be imaged simultaneously; the sketch shows only the simple case of a binary off-axis Fresnel zone lens, which channels the light mainly into two diffraction orders. Right: optimized hologram used for experiments; a section is magnified to resolve its fine structure.

Fig. 2
Fig. 2

Results from the imaging of a clubmoss spore; (a) distribution of imaged planes; (b) raw image at the camera; (c) frames extracted from the multi-color image ( Media 1).

Fig. 3
Fig. 3

Comparison of a single-shot multicolor recording with a sequentially recorded image stack (using green illumination); the sample consists of PMMA beads in agarose gel; (a) transverse intensity profiles along the same line through the sample (marked in the inset Fig.); the frame taken from the multicolor-stack (red plot) and an independently taken acquisition of the same focal plane (blue plot) show similar contrasts; (b) axial sections through the sequentially acquired stack (upper image) and the single-shot multicolor recording; both images show comparable information; slight contrast differences and misalignments between the individual frames of the single-shot recording are noticeable.

Fig. 4
Fig. 4

Beam geometry in diffractive multi-plane imaging; each sub-image that is created by diffraction is represented by a separate light cone (here only two cones are shown for the sake of simplicity). In order to avoid overlapping of the cones one must either increase the diffraction angle α or narrow the FOV with a diaphragm in the intermediate image plane. The maximal possible diffraction angle αmax determines a maximum for the FOV.

Fig. 5
Fig. 5

Maps of accessible focus ranges: (a) The lines mark the maximal focal shifts that can be realized with pixelated diffractive lenses as a function of the objective NA. Three different values for the period (in pixels) of a grating lying on top of the lens have been considered, assuming a refractive index of n = 1.33; (b) accessible focusing range as function of the lateral image shift; if both lateral axes are considered, the focusing range defines a diamond-shaped volume (inset); objective pupil apodizations due to diffractive losses have been calculated for four different scenarios. The corresponding intensity distributions in the objective pupil are shown on the right, with the average diffraction efficiencies denoted in the pupil centers. (c) two examples for the distribution of multiple focal planes. The planes can be freely arranged within the diamond-shaped volume, but their projections onto the x- and y-axes must not overlap. The plane in the center of both examples is the zero diffraction order image which cannot be moved. All calculations presented in this figure assumed a wavelength of 532 nm, a hologram measuring 1080 pixels in diameter and matching refractive indices of sample and immersion medium.

Equations (10)

Equations on this page are rendered with MathJax. Learn more.

N P S F = FOV ( λ 0 2 NA ) .
N P S F = N δ ( λ 0 2 sin ( β / 2 ) ) ,
N P S F N 2
Δ λ 0 λ 0 ( N 2 ) .
| Δ z | ( 1 2 1 P ) N λ 0 2 NA 2 n 2 NA 2 .
D ( ρ ) = 2 π λ 0 NA n 2 NA 2 ρ 2 Δ z ,
| d D ( ρ ) d ρ | ( ρ = 1 ) π N 2 .
| Δ z | 1 4 N λ 0 NA 2 n 2 NA 2 .
| d d ξ ( D ( ξ ) + k ξ ) | ( ξ = 1 ) π N 2 .
| Δ z | ( 1 2 1 P ) N λ 0 2 NA 2 n 2 NA 2 ,

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