Abstract

Line illumination geometries have advantageous properties for temporal focusing nonlinear microscopy. The characteristics of line temporal focusing (LITEF) in transparent and scattering media are studied here both experimentally and using numerical model simulations. We introduce an approximate analytical formula for the dependence of axial sectioning on the laser and microscope's parameters. Furthermore, we show that LITEF is more robust to tissue scattering than wide-field temporal focusing, and can penetrate much deeper into scattering tissue while maintaining good sectioning capabilities. Based on these observations, we propose a new design for LITEF-based tissue imaging at depths that could potentially exceed the out-of-focus physical excitation limit.

© 2013 OSA

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  1. D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express13(5), 1468–1476 (2005).
    [CrossRef] [PubMed]
  2. G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express13(6), 2153–2159 (2005).
    [CrossRef] [PubMed]
  3. E. Tal, D. Oron, and Y. Silberberg, “Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing,” Opt. Lett.30(13), 1686–1688 (2005).
    [CrossRef] [PubMed]
  4. E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express17(7), 5391–5401 (2009).
    [CrossRef] [PubMed]
  5. H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express19(6), 4937–4948 (2011).
    [CrossRef] [PubMed]
  6. O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express2(3), 696–704 (2011).
    [CrossRef] [PubMed]
  7. L. C. Cheng, C. Y. Chang, C. Y. Lin, K. C. Cho, W. C. Yen, N. S. Chang, C. Xu, C. Y. Dong, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express20(8), 8939–8948 (2012).
    [CrossRef] [PubMed]
  8. H. Dana, A. Marom, N. Kruger, A. Ellman, and S. Shoham. “Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization,” Proc. SPIE822603 (2012).
  9. J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
    [CrossRef] [PubMed]
  10. A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
    [CrossRef] [PubMed]
  11. A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
    [CrossRef] [PubMed]
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    [CrossRef]
  13. D. N. Vitek, D. E. Adams, A. Johnson, P. S. Tsai, S. Backus, C. G. Durfee, D. Kleinfeld, and J. A. Squier, “Temporally focused femtosecond laser pulses for low numerical aperture micromachining through optically transparent materials,” Opt. Express18(17), 18086–18094 (2010).
    [CrossRef] [PubMed]
  14. D. Kim and P. T. C. So, “High-throughput three-dimensional lithographic microfabrication,” Opt. Lett.35(10), 1602–1604 (2010).
    [CrossRef] [PubMed]
  15. D. N. Vitek, E. Block, Y. Bellouard, D. E. Adams, S. Backus, D. Kleinfeld, C. G. Durfee, and J. A. Squier, “Spatio-temporally focused femtosecond laser pulses for nonreciprocal writing in optically transparent materials,” Opt. Express18(24), 24673–24678 (2010).
    [CrossRef] [PubMed]
  16. F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
    [CrossRef]
  17. E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
    [CrossRef] [PubMed]
  18. B. K. Andrasfalvy, B. V. Zemelman, J. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A.107(26), 11981–11986 (2010).
    [CrossRef] [PubMed]
  19. M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing for axial scanning,” Opt. Express14(25), 12243–12254 (2006).
    [CrossRef] [PubMed]
  20. H. Dana and S. Shoham, “Remotely scanned multiphoton temporal focusing by axial grism scanning,” Opt. Lett.37(14), 2913–2915 (2012).
    [CrossRef] [PubMed]
  21. P. Theer and W. Denk, “On the fundamental imaging-depth limit in two-photon microscopy,” J. Opt. Soc. Am. A23(12), 3139–3149 (2006).
    [CrossRef] [PubMed]
  22. Q. Fang and D. A. Boas, “Monte Carlo simulation of photon migration in 3D turbid media accelerated by graphics processing units,” Opt. Express17(22), 20178–20190 (2009).
    [CrossRef] [PubMed]
  23. F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods2(12), 932–940 (2005).
    [CrossRef] [PubMed]
  24. C. G. Durfee, M. Greco, E. Block, D. Vitek, and J. A. Squier, “Intuitive analysis of space-time focusing with double-ABCD calculation,” Opt. Express20(13), 14244–14259 (2012).
    [CrossRef] [PubMed]
  25. E. Papagiakoumou, A. Bègue, O. Schwartz, D. Oron, and V. Emiliani, “Shaped two-photon excitation deep inside scattering tissue,” arXiv preprint arXiv:1109.0160 (2011).

2012

2011

O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express2(3), 696–704 (2011).
[CrossRef] [PubMed]

H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express19(6), 4937–4948 (2011).
[CrossRef] [PubMed]

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

2010

2009

2008

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

2006

2005

Adams, D. E.

Andrasfalvy, B. K.

B. K. Andrasfalvy, B. V. Zemelman, J. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A.107(26), 11981–11986 (2010).
[CrossRef] [PubMed]

Anselmi, F.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Aubé, B.

Backus, S.

Bègue, A.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Bellouard, Y.

Blake, G. A.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Block, E.

Boas, D. A.

Chang, C. Y.

Chang, N. S.

Chen, S. J.

Chen, Y.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Cheng, L. C.

Cheng, Y.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Cho, K. C.

Côté, D.

Dana, H.

Davidson, M. W.

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

de Sars, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express17(7), 5391–5401 (2009).
[CrossRef] [PubMed]

Denk, W.

Dong, C. Y.

Durfee, C. G.

Durst, M.

Durst, M. E.

Ellman, A.

H. Dana, A. Marom, N. Kruger, A. Ellman, and S. Shoham. “Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization,” Proc. SPIE822603 (2012).

Emiliani, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express17(7), 5391–5401 (2009).
[CrossRef] [PubMed]

Fang, Q.

Ghitani, A.

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

Glückstad, J.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Greco, M.

Guo, C. L.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

He, F.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Helmchen, F.

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods2(12), 932–940 (2005).
[CrossRef] [PubMed]

Holland, D. B.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Isacoff, E. Y.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

Johnson, A.

Kim, D.

Kleinfeld, D.

Koninck, P. D.

Kruger, N.

H. Dana, A. Marom, N. Kruger, A. Ellman, and S. Shoham. “Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization,” Proc. SPIE822603 (2012).

Kuo, C. H.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Lin, C. Y.

Lin, J.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Marom, A.

H. Dana, A. Marom, N. Kruger, A. Ellman, and S. Shoham. “Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization,” Proc. SPIE822603 (2012).

Midorikawa, K.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Ni, J.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Oron, D.

Ouyang, M.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Pagès, S.

Papagiakoumou, E.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express17(7), 5391–5401 (2009).
[CrossRef] [PubMed]

Shank, C. V.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Shoham, S.

Shroff, H.

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Silberberg, Y.

So, P. T. C.

Squier, J. A.

Sugioka, K.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Tal, E.

Tang, J.

B. K. Andrasfalvy, B. V. Zemelman, J. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A.107(26), 11981–11986 (2010).
[CrossRef] [PubMed]

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Theer, P.

Therrien, O. D.

Tsai, P. S.

van Howe, J.

Vaziri, A.

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

B. K. Andrasfalvy, B. V. Zemelman, J. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A.107(26), 11981–11986 (2010).
[CrossRef] [PubMed]

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

Vitek, D.

Vitek, D. N.

Xu, C.

Xu, Z.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Yen, W. C.

York, A. G.

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

Yu, J. Y.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Zadoyan, R.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

Zemelman, B. V.

B. K. Andrasfalvy, B. V. Zemelman, J. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A.107(26), 11981–11986 (2010).
[CrossRef] [PubMed]

Zhu, G.

Zipfel, W.

Biomed. Opt. Express

J. Biomed. Opt.

J. Y. Yu, C. H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C. L. Guo, “Wide-field optical sectioning for live-tissue imaging by plane-projection multiphoton microscopy,” J. Biomed. Opt.16(11), 116009 (2011).
[CrossRef] [PubMed]

J. Opt. Soc. Am. A

J. Opt. Soc. Am. B

Nat. Methods

A. G. York, A. Ghitani, A. Vaziri, M. W. Davidson, and H. Shroff, “Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes,” Nat. Methods8(4), 327–333 (2011).
[CrossRef] [PubMed]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods7(10), 848–854 (2010).
[CrossRef] [PubMed]

F. Helmchen and W. Denk, “Deep tissue two-photon microscopy,” Nat. Methods2(12), 932–940 (2005).
[CrossRef] [PubMed]

New J. Phys.

F. He, Y. Cheng, J. Lin, J. Ni, Z. Xu, K. Sugioka, and K. Midorikawa, “Independent control of aspect ratios in the axial and lateral cross sections of a focal spot for three-dimensional femtosecond laser micromachining,” New J. Phys.13(8), 083014 (2011).
[CrossRef]

Opt. Express

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express13(5), 1468–1476 (2005).
[CrossRef] [PubMed]

G. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express13(6), 2153–2159 (2005).
[CrossRef] [PubMed]

M. E. Durst, G. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing for axial scanning,” Opt. Express14(25), 12243–12254 (2006).
[CrossRef] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express17(7), 5391–5401 (2009).
[CrossRef] [PubMed]

Q. Fang and D. A. Boas, “Monte Carlo simulation of photon migration in 3D turbid media accelerated by graphics processing units,” Opt. Express17(22), 20178–20190 (2009).
[CrossRef] [PubMed]

H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express19(6), 4937–4948 (2011).
[CrossRef] [PubMed]

L. C. Cheng, C. Y. Chang, C. Y. Lin, K. C. Cho, W. C. Yen, N. S. Chang, C. Xu, C. Y. Dong, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express20(8), 8939–8948 (2012).
[CrossRef] [PubMed]

C. G. Durfee, M. Greco, E. Block, D. Vitek, and J. A. Squier, “Intuitive analysis of space-time focusing with double-ABCD calculation,” Opt. Express20(13), 14244–14259 (2012).
[CrossRef] [PubMed]

D. N. Vitek, D. E. Adams, A. Johnson, P. S. Tsai, S. Backus, C. G. Durfee, D. Kleinfeld, and J. A. Squier, “Temporally focused femtosecond laser pulses for low numerical aperture micromachining through optically transparent materials,” Opt. Express18(17), 18086–18094 (2010).
[CrossRef] [PubMed]

D. N. Vitek, E. Block, Y. Bellouard, D. E. Adams, S. Backus, D. Kleinfeld, C. G. Durfee, and J. A. Squier, “Spatio-temporally focused femtosecond laser pulses for nonreciprocal writing in optically transparent materials,” Opt. Express18(24), 24673–24678 (2010).
[CrossRef] [PubMed]

Opt. Lett.

Proc. Natl. Acad. Sci. U.S.A.

A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A.105(51), 20221–20226 (2008).
[CrossRef] [PubMed]

B. K. Andrasfalvy, B. V. Zemelman, J. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A.107(26), 11981–11986 (2010).
[CrossRef] [PubMed]

Proc. SPIE

H. Dana, A. Marom, N. Kruger, A. Ellman, and S. Shoham. “Rapid volumetric temporal focusing multiphoton microscopy of neural activity: theory, image processing, and experimental realization,” Proc. SPIE822603 (2012).

Other

E. Papagiakoumou, A. Bègue, O. Schwartz, D. Oron, and V. Emiliani, “Shaped two-photon excitation deep inside scattering tissue,” arXiv preprint arXiv:1109.0160 (2011).

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