Abstract

The in-vivo investigation of highly dynamic biological samples, for example the beating zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is ideal for such samples as it records high-contrast images of entire planes within large samples at once. However, in order to obtain images of different planes, it has been necessary to move the sample relative to the fixed focal plane of the detection objective lens. This mechanical movement limits speed, precision and may be harmful to the sample. We have built a light-sheet microscope that uses remote focusing with an electrically tunable lens (ETL). Without moving specimen or objective we have thereby achieved flexible volume imaging at much higher speeds than previously reported. Our high-speed microscope delivers 3D snapshots of sensitive biological samples. As an example, we imaged 17 planes within a beating zebrafish heart at 510 frames per second, equivalent to 30 volume scans per second. Movements, shape changes and signals across the entire volume can be followed which has been impossible with existing reconstruction techniques.

© 2013 OSA

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2013 (2)

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

C.  Skupsch, C.  Brücker, “Multiple-plane particle image velocimetry using a light-field camera,” Opt. Express 21, 1726–1740 (2013).
[CrossRef] [PubMed]

2012 (3)

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

A.  Kaufmann, M.  Mickoleit, M.  Weber, J.  Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[CrossRef] [PubMed]

2011 (2)

B. F.  Grewe, F. F.  Voigt, M.  van’t Hoff, F.  Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Exp. 2, 2035–2046 (2011).
[CrossRef]

S.  Kumar, D.  Wilding, M. B.  Sikkel, A. R.  Lyon, K. T.  MacLeod, C.  Dunsby, “High-speed 2D and 3D fluorescence microscopy of cardiac myocytes,” Opt. Express 19, 13839–13847 (2011).
[CrossRef] [PubMed]

2010 (1)

2009 (3)

C.  Dunsby, “Optically sectioned imaging by oblique plane microscopy,” Opt. Express 16, 20306–20316 (2009).
[CrossRef]

J.  Huisken, D. Y. R.  Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[CrossRef] [PubMed]

M.  Levoy, Z.  Zhang, I.  McDowall, “Recording and controlling the 4D light field in a microscope using microlens arrays,” J. Mic. 235, 144–162 (2009).
[CrossRef]

2008 (3)

E. J.  Botcherby, R.  Juškaitis, M. J.  Booth, T.  Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281, 880–887 (2008).
[CrossRef]

T. F.  Holekamp, D.  Turaga, T. E.  Holy, “Fast Three-Dimensional Fluorescence Imaging of Activity in Neural Populations by Objective-Coupled Planar Illumination Microscopy,” Neuron 57, 661–672 (2008).
[CrossRef] [PubMed]

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

2007 (1)

2006 (1)

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

2004 (1)

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Abrahamsson, S.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Agard, D. A.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Ankerhold, R.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Bargmann, C. I.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Booth, M. J.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

E. J.  Botcherby, R.  Juškaitis, M. J.  Booth, T.  Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281, 880–887 (2008).
[CrossRef]

Botcherby, E. J.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

E. J.  Botcherby, R.  Juškaitis, M. J.  Booth, T.  Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281, 880–887 (2008).
[CrossRef]

Brücker, C.

Candelier, R.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Chao, J.

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

Chen, J.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Dahan, M.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Dalgarno, H. I. C.

Dalgarno, P. A.

Darzacq, X.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Débarre, D.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

Debrégeas, G.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Del Bene, F.

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Dickinson, M. E.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Dugast Darzacq, C.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Dunsby, C.

Forouhar, A. S.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Fraser, S. E.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Gharib, M.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Greenaway, A. H.

Grewe, B. F.

B. F.  Grewe, F. F.  Voigt, M.  van’t Hoff, F.  Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Exp. 2, 2035–2046 (2011).
[CrossRef]

Gustafsson, M. G. L.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Hajj, B.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Helmchen, F.

B. F.  Grewe, F. F.  Voigt, M.  van’t Hoff, F.  Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Exp. 2, 2035–2046 (2011).
[CrossRef]

Holekamp, T. F.

T. F.  Holekamp, D.  Turaga, T. E.  Holy, “Fast Three-Dimensional Fluorescence Imaging of Activity in Neural Populations by Objective-Coupled Planar Illumination Microscopy,” Neuron 57, 661–672 (2008).
[CrossRef] [PubMed]

Holy, T. E.

T. F.  Holekamp, D.  Turaga, T. E.  Holy, “Fast Three-Dimensional Fluorescence Imaging of Activity in Neural Populations by Objective-Coupled Planar Illumination Microscopy,” Neuron 57, 661–672 (2008).
[CrossRef] [PubMed]

Huisken, J.

A.  Kaufmann, M.  Mickoleit, M.  Weber, J.  Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[CrossRef] [PubMed]

J.  Huisken, D. Y. R.  Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[CrossRef] [PubMed]

J.  Huisken, D. Y. R.  Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32, 2608–2610 (2007).
[CrossRef] [PubMed]

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Juškaitis, R.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

E. J.  Botcherby, R.  Juškaitis, M. J.  Booth, T.  Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281, 880–887 (2008).
[CrossRef]

Katsov, A. Y.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Kaufmann, A.

A.  Kaufmann, M.  Mickoleit, M.  Weber, J.  Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[CrossRef] [PubMed]

Kohl, M. M.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

Kumar, S.

Lambert, R.

Levoy, M.

M.  Levoy, Z.  Zhang, I.  McDowall, “Recording and controlling the 4D light field in a microscope using microlens arrays,” J. Mic. 235, 144–162 (2009).
[CrossRef]

Liebling, M.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Logan, D. C.

Lyon, A. R.

MacLeod, K. T.

McDowall, I.

M.  Levoy, Z.  Zhang, I.  McDowall, “Recording and controlling the 4D light field in a microscope using microlens arrays,” J. Mic. 235, 144–162 (2009).
[CrossRef]

Mickoleit, M.

A.  Kaufmann, M.  Mickoleit, M.  Weber, J.  Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[CrossRef] [PubMed]

Mizuguchi, G.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Mueller, F.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Ober, R. J.

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

Olive, R.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Panier, T.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Paterson, L.

Paulsen, O.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

Pietri, T.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Prabhat, P.

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

Putoud, A.

Ram, S.

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

Romano, S.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Sikkel, M. B.

Skupsch, C.

Smith, C. W.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

Soule, P.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Stainier, D. Y. R.

J.  Huisken, D. Y. R.  Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[CrossRef] [PubMed]

J.  Huisken, D. Y. R.  Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32, 2608–2610 (2007).
[CrossRef] [PubMed]

Stallinga, S.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Stelzer, E. H. K.

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Sumbre, G.

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

Swoger, J.

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Towers, D. P.

Turaga, D.

T. F.  Holekamp, D.  Turaga, T. E.  Holy, “Fast Three-Dimensional Fluorescence Imaging of Activity in Neural Populations by Objective-Coupled Planar Illumination Microscopy,” Neuron 57, 661–672 (2008).
[CrossRef] [PubMed]

van’t Hoff, M.

B. F.  Grewe, F. F.  Voigt, M.  van’t Hoff, F.  Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Exp. 2, 2035–2046 (2011).
[CrossRef]

Voigt, F. F.

B. F.  Grewe, F. F.  Voigt, M.  van’t Hoff, F.  Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Exp. 2, 2035–2046 (2011).
[CrossRef]

Warburton, R. J.

Ward, E. S.

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

Weber, M.

A.  Kaufmann, M.  Mickoleit, M.  Weber, J.  Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[CrossRef] [PubMed]

Wilding, D.

Wilson, T.

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

E. J.  Botcherby, R.  Juškaitis, M. J.  Booth, T.  Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281, 880–887 (2008).
[CrossRef]

Wisniewski, J.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Wittbrodt, J.

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Wolleschensky, R.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Wu, C.

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Zhang, Z.

M.  Levoy, Z.  Zhang, I.  McDowall, “Recording and controlling the 4D light field in a microscope using microlens arrays,” J. Mic. 235, 144–162 (2009).
[CrossRef]

Zimmermann, B.

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Biomed. Opt. Exp. (1)

B. F.  Grewe, F. F.  Voigt, M.  van’t Hoff, F.  Helmchen, “Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens,” Biomed. Opt. Exp. 2, 2035–2046 (2011).
[CrossRef]

Biophys. J. (1)

S.  Ram, P.  Prabhat, J.  Chao, E. S.  Ward, R. J.  Ober, “High Accuracy 3D Quantum Dot Tracking with Multifocal Plane Microscopy for the Study of Fast Intracellular Dynamics in Live Cells,” Biophys. J. 95, 6025–6043 (2008).
[CrossRef] [PubMed]

Dev. Dynam. (1)

M.  Liebling, A. S.  Forouhar, R.  Wolleschensky, B.  Zimmermann, R.  Ankerhold, S. E.  Fraser, M.  Gharib, M. E.  Dickinson, “Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development,” Dev. Dynam. 235, 2940–2948 (2006).
[CrossRef]

Development (2)

J.  Huisken, D. Y. R.  Stainier, “Selective plane illumination microscopy techniques in developmental biology,” Development 136, 1963–1975 (2009).
[CrossRef] [PubMed]

A.  Kaufmann, M.  Mickoleit, M.  Weber, J.  Huisken, “Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope,” Development 139, 3242–3247 (2012).
[CrossRef] [PubMed]

Front. Neural Circuits (1)

T.  Panier, S.  Romano, R.  Olive, T.  Pietri, G.  Sumbre, R.  Candelier, G.  Debrégeas, “Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy,” Front. Neural Circuits 7, 65 (2013).
[CrossRef] [PubMed]

J. Mic. (1)

M.  Levoy, Z.  Zhang, I.  McDowall, “Recording and controlling the 4D light field in a microscope using microlens arrays,” J. Mic. 235, 144–162 (2009).
[CrossRef]

Nat. Methods (1)

S.  Abrahamsson, J.  Chen, B.  Hajj, S.  Stallinga, A. Y.  Katsov, J.  Wisniewski, G.  Mizuguchi, P.  Soule, F.  Mueller, C.  Dugast Darzacq, X.  Darzacq, C.  Wu, C. I.  Bargmann, D. A.  Agard, M.  Dahan, M. G. L.  Gustafsson, “Fast multicolor 3D imaging using aberration-corrected multifocus microscopy,” Nat. Methods 10, 60–63 (2012).
[CrossRef] [PubMed]

Neuron (1)

T. F.  Holekamp, D.  Turaga, T. E.  Holy, “Fast Three-Dimensional Fluorescence Imaging of Activity in Neural Populations by Objective-Coupled Planar Illumination Microscopy,” Neuron 57, 661–672 (2008).
[CrossRef] [PubMed]

Opt. Commun. (1)

E. J.  Botcherby, R.  Juškaitis, M. J.  Booth, T.  Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281, 880–887 (2008).
[CrossRef]

Opt. Express (4)

Opt. Lett. (1)

P. Natl Acad. Sci. USA (1)

E. J.  Botcherby, C. W.  Smith, M. M.  Kohl, D.  Débarre, M. J.  Booth, R.  Juškaitis, O.  Paulsen, T.  Wilson, “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” P. Natl Acad. Sci. USA 109, 2919–2924 (2012).
[CrossRef]

Science (1)

J.  Huisken, J.  Swoger, F.  Del Bene, J.  Wittbrodt, E. H. K.  Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[CrossRef] [PubMed]

Supplementary Material (4)

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