Abstract

The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm2 at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.

© 2013 OSA

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2013 (2)

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

A. Orth and K. Crozier, “Gigapixel fluorescence microscopy with a water immersion microlens array,” Opt. Express21(2), 2361–2368 (2013).
[CrossRef] [PubMed]

2012 (3)

2011 (2)

S. Pang, C. Han, L. M. Lee, and C. H. Yang, “Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope,” Lab Chip11(21), 3698–3702 (2011).
[CrossRef] [PubMed]

J. G. Wu, G. A. Zheng, Z. Li, and C. H. Yang, “Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination,” Opt. Lett.36(12), 2179–2181 (2011).
[CrossRef] [PubMed]

2010 (2)

J. Wu, X. Cui, G. Zheng, Y. M. Wang, L. M. Lee, and C. Yang, “Wide field-of-view microscope based on holographic focus grid illumination,” Opt. Lett.35(13), 2188–2190 (2010).
[CrossRef] [PubMed]

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

2006 (1)

F. Pfeiffer, T. Weitkamp, O. Bunk, and C. David, “Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources,” Nat. Phys.2(4), 258–261 (2006).
[CrossRef]

2005 (1)

A. Olszak and M. Descour, “Microscopy in multiples,” SPIE oemagazine, May 2005, 16–18 (2005), doi: .
[CrossRef]

2003 (1)

C.-D. Hu and T. K. Kerppola, “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis,” Nat. Biotechnol.21(5), 539–545 (2003).
[CrossRef] [PubMed]

2000 (2)

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

1997 (1)

H. D. Luján, M. R. Mowatt, and T. E. Nash, “Mechanisms of Giardia lamblia differentiation into cysts,” Microbiol. Mol. Biol. Rev.61(3), 294–304 (1997).
[PubMed]

1990 (1)

1971 (1)

A. W. Lohmann and D. E. Silva, “An interferometer based on the Talbot effect,” Opt. Commun.2(9), 413–415 (1971).
[CrossRef]

1967 (1)

Bernstein, M.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Bower, D. V.

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

Bulkescher, J.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Bunk, O.

F. Pfeiffer, T. Weitkamp, O. Bunk, and C. David, “Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources,” Nat. Phys.2(4), 258–261 (2006).
[CrossRef]

Cetin, C.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Chapuis, C.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Conrad, C.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Crozier, K.

Cui, X.

David, C.

F. Pfeiffer, T. Weitkamp, O. Bunk, and C. David, “Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources,” Nat. Phys.2(4), 258–261 (2006).
[CrossRef]

Descour, M.

A. Olszak and M. Descour, “Microscopy in multiples,” SPIE oemagazine, May 2005, 16–18 (2005), doi: .
[CrossRef]

Durbin, R.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Eils, R.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Ellenberg, J.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Erfle, H.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Feng, G.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Gerlich, D. W.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Han, C.

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

S. Pang, C. Han, M. Kato, P. W. Sternberg, and C. H. Yang, “Wide and scalable field-of-view Talbot-grid-based fluorescence microscopy,” Opt. Lett.37(23), 5018–5020 (2012).
[CrossRef] [PubMed]

S. Pang, C. Han, L. M. Lee, and C. H. Yang, “Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope,” Lab Chip11(21), 3698–3702 (2011).
[CrossRef] [PubMed]

Hattori, S.

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

Held, M.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Hériché, J. K.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Hirose, S.

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

Hu, C.-D.

C.-D. Hu and T. K. Kerppola, “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis,” Nat. Biotechnol.21(5), 539–545 (2003).
[CrossRef] [PubMed]

Huber, W.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Hulsken, B.

B. Hulsken, D. Vossen, and S. Stallinga, “High NA diffractive array illuminators and application in a multi-spot scanning microscope,” J. European Opt. Soc.-Rapid Pub.7, 12026 (2012).
[CrossRef]

Hyman, A. A.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Kabbe, R.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Kato, M.

Keller-Peck, C.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Kerppola, T. K.

C.-D. Hu and T. K. Kerppola, “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis,” Nat. Biotechnol.21(5), 539–545 (2003).
[CrossRef] [PubMed]

Lee, L. M.

S. Pang, C. Han, L. M. Lee, and C. H. Yang, “Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope,” Lab Chip11(21), 3698–3702 (2011).
[CrossRef] [PubMed]

J. Wu, X. Cui, G. Zheng, Y. M. Wang, L. M. Lee, and C. Yang, “Wide field-of-view microscope based on holographic focus grid illumination,” Opt. Lett.35(13), 2188–2190 (2010).
[CrossRef] [PubMed]

Li, Z.

Lichtman, J. W.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Liebel, U.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Lohmann, A. W.

A. W. Lohmann and J. A. Thomas, “Making an array illuminator based on the Talbot effect,” Appl. Opt.29(29), 4337–4340 (1990).
[CrossRef] [PubMed]

A. W. Lohmann and D. E. Silva, “An interferometer based on the Talbot effect,” Opt. Commun.2(9), 413–415 (1971).
[CrossRef]

Luján, H. D.

H. D. Luján, M. R. Mowatt, and T. E. Nash, “Mechanisms of Giardia lamblia differentiation into cysts,” Microbiol. Mol. Biol. Rev.61(3), 294–304 (1997).
[PubMed]

Mellor, R. H.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Montgomery, W. D.

Mowatt, M. R.

H. D. Luján, M. R. Mowatt, and T. E. Nash, “Mechanisms of Giardia lamblia differentiation into cysts,” Microbiol. Mol. Biol. Rev.61(3), 294–304 (1997).
[PubMed]

Nash, T. E.

H. D. Luján, M. R. Mowatt, and T. E. Nash, “Mechanisms of Giardia lamblia differentiation into cysts,” Microbiol. Mol. Biol. Rev.61(3), 294–304 (1997).
[PubMed]

Nerbonne, J. M.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Neumann, B.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Nguyen, Q. T.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Nishimura, H.

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

Okumura, K.

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

Olszak, A.

A. Olszak and M. Descour, “Microscopy in multiples,” SPIE oemagazine, May 2005, 16–18 (2005), doi: .
[CrossRef]

Orth, A.

Pang, S.

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

S. Pang, C. Han, M. Kato, P. W. Sternberg, and C. H. Yang, “Wide and scalable field-of-view Talbot-grid-based fluorescence microscopy,” Opt. Lett.37(23), 5018–5020 (2012).
[CrossRef] [PubMed]

S. Pang, C. Han, L. M. Lee, and C. H. Yang, “Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope,” Lab Chip11(21), 3698–3702 (2011).
[CrossRef] [PubMed]

Pau, G.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Pepperkok, R.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Peters, J. M.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Pfeiffer, F.

F. Pfeiffer, T. Weitkamp, O. Bunk, and C. David, “Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources,” Nat. Phys.2(4), 258–261 (2006).
[CrossRef]

Poser, I.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Rogers, P.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Sanes, J. R.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Satagopam, V.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Schmitz, M. H. A.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Schneider, R.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Shirai, T.

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

Sieckmann, F.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Silva, D. E.

A. W. Lohmann and D. E. Silva, “An interferometer based on the Talbot effect,” Opt. Commun.2(9), 413–415 (1971).
[CrossRef]

Stallinga, S.

B. Hulsken, D. Vossen, and S. Stallinga, “High NA diffractive array illuminators and application in a multi-spot scanning microscope,” J. European Opt. Soc.-Rapid Pub.7, 12026 (2012).
[CrossRef]

Sternberg, P. W.

Thomas, J. A.

Tsurui, H.

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

Vossen, D.

B. Hulsken, D. Vossen, and S. Stallinga, “High NA diffractive array illuminators and application in a multi-spot scanning microscope,” J. European Opt. Soc.-Rapid Pub.7, 12026 (2012).
[CrossRef]

Wallace, M.

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Walter, T.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Wang, Y. M.

Weitkamp, T.

F. Pfeiffer, T. Weitkamp, O. Bunk, and C. David, “Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources,” Nat. Phys.2(4), 258–261 (2006).
[CrossRef]

Wu, J.

Wu, J. G.

Wünsche, A.

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Yang, C.

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

J. Wu, X. Cui, G. Zheng, Y. M. Wang, L. M. Lee, and C. Yang, “Wide field-of-view microscope based on holographic focus grid illumination,” Opt. Lett.35(13), 2188–2190 (2010).
[CrossRef] [PubMed]

Yang, C. H.

Yiu, P.

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

Zheng, G.

Zheng, G. A.

Anal. Chem. (1)

C. Han, S. Pang, D. V. Bower, P. Yiu, and C. Yang, “Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator,” Anal. Chem.85(4), 2356–2360 (2013).
[CrossRef] [PubMed]

Appl. Opt. (1)

J. European Opt. Soc.-Rapid Pub. (1)

B. Hulsken, D. Vossen, and S. Stallinga, “High NA diffractive array illuminators and application in a multi-spot scanning microscope,” J. European Opt. Soc.-Rapid Pub.7, 12026 (2012).
[CrossRef]

J. Histochem. Cytochem. (1)

H. Tsurui, H. Nishimura, S. Hattori, S. Hirose, K. Okumura, and T. Shirai, “Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition,” J. Histochem. Cytochem.48(5), 653–662 (2000).
[CrossRef] [PubMed]

J. Opt. Soc. Am. (1)

Lab Chip (1)

S. Pang, C. Han, L. M. Lee, and C. H. Yang, “Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope,” Lab Chip11(21), 3698–3702 (2011).
[CrossRef] [PubMed]

Microbiol. Mol. Biol. Rev. (1)

H. D. Luján, M. R. Mowatt, and T. E. Nash, “Mechanisms of Giardia lamblia differentiation into cysts,” Microbiol. Mol. Biol. Rev.61(3), 294–304 (1997).
[PubMed]

Nat. Biotechnol. (1)

C.-D. Hu and T. K. Kerppola, “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis,” Nat. Biotechnol.21(5), 539–545 (2003).
[CrossRef] [PubMed]

Nat. Phys. (1)

F. Pfeiffer, T. Weitkamp, O. Bunk, and C. David, “Phase retrieval and differential phase-contrast imaging with low-brilliance X-ray sources,” Nat. Phys.2(4), 258–261 (2006).
[CrossRef]

Nature (1)

B. Neumann, T. Walter, J. K. Hériché, J. Bulkescher, H. Erfle, C. Conrad, P. Rogers, I. Poser, M. Held, U. Liebel, C. Cetin, F. Sieckmann, G. Pau, R. Kabbe, A. Wünsche, V. Satagopam, M. H. A. Schmitz, C. Chapuis, D. W. Gerlich, R. Schneider, R. Eils, W. Huber, J. M. Peters, A. A. Hyman, R. Durbin, R. Pepperkok, and J. Ellenberg, “Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes,” Nature464(7289), 721–727 (2010).
[CrossRef] [PubMed]

Neuron (1)

G. Feng, R. H. Mellor, M. Bernstein, C. Keller-Peck, Q. T. Nguyen, M. Wallace, J. M. Nerbonne, J. W. Lichtman, and J. R. Sanes, “Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP,” Neuron28(1), 41–51 (2000).
[CrossRef] [PubMed]

Opt. Commun. (1)

A. W. Lohmann and D. E. Silva, “An interferometer based on the Talbot effect,” Opt. Commun.2(9), 413–415 (1971).
[CrossRef]

Opt. Express (2)

Opt. Lett. (3)

SPIE oemagazine (1)

A. Olszak and M. Descour, “Microscopy in multiples,” SPIE oemagazine, May 2005, 16–18 (2005), doi: .
[CrossRef]

Other (3)

Hamamastu NanoZoomer 2.0-HT Catalog”, retrieved http://jp.hamamatsu.com/resources/products/sys/pdf/eng/e_ndp20.pdf .

J. Pawley, Handbook of Biological Confocal Microscopy (Springer, 2006).

W. J. Smith and I. Genesee, Optics Software, Modern Lens Design: A Resource Manual (McGraw-Hill, 1992).

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