Abstract

We report the design and implementation of a multispectral imaging Fourier transform endospectroscopy (IFTES) system. The IFTES system employs a flexible fiber bundle catheter coupled to a home-built imaging Fourier transform spectroscope. The instrument enables the performance of non- or minimally invasive subsurface imaging and multispectral imaging at the cellular level in vivo and in situ. A maximum spectral resolution of 0.2 nm at 632.8 nm and a lateral resolution of 4.4 μm were proved. Preliminary results of a standard resolution target, ex-vivo small animal tissue, single wavelength laser, fluorescence solution, in-vivo mouse skin, microspheres mixture, and in-vivo transgenic mouse brain were given to demonstrate the potential of the technique.

© 2012 OSA

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2012 (2)

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105–021107 (2012).
[CrossRef] [PubMed]

N. Hagen, R. T. Kester, L. Gao, and T. S. Tkaczyk, “Snapshot advantage: a review of the light collection improvement for parallel high-dimensional measurement systems,” Opt. Eng.51(11), 111702 (2012).
[CrossRef] [PubMed]

2011 (1)

R. T. Kester, N. Bedard, L. Gao, and T. S. Tkaczyk, “Real-time snapshot hyperspectral imaging endoscope,” J. Biomed. Opt.16(5), 056005 (2011).
[CrossRef] [PubMed]

2010 (6)

C. J. Engelbrecht, F. Voigt, and F. Helmchen, “Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging,” Opt. Lett.35(9), 1413–1415 (2010).
[CrossRef] [PubMed]

G. O. Fruhwirth, S. Ameer-Beg, R. Cook, T. Watson, T. Ng, and F. Festy, “Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells,” Opt. Express18(11), 11148–11158 (2010).
[CrossRef] [PubMed]

L. A. Gao, R. T. Kester, N. Hagen, and T. S. Tkaczyk, “Snapshot image mapping spectrometer (IMS) with high sampling density for hyperspectral microscopy,” Opt. Express18(14), 14330–14344 (2010).
[CrossRef] [PubMed]

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

C. Porrero, P. Rubio-Garrido, C. Avendaño, and F. Clascá, “Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice,” Brain Res.1345, 59–72 (2010).
[CrossRef] [PubMed]

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

2009 (4)

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

G. D. De Palma, “Confocal laser endomicroscopy in the “in vivo” histological diagnosis of the gastrointestinal tract,” World J. Gastroenterol.15(46), 5770–5775 (2009).
[CrossRef] [PubMed]

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Y. Sun, J. Phipps, D. S. Elson, H. Stoy, S. Tinling, J. Meier, B. Poirier, F. S. Chuang, D. G. Farwell, and L. Marcu, “Fluorescence lifetime imaging microscopy: in vivo application to diagnosis of oral carcinoma,” Opt. Lett.34(13), 2081–2083 (2009).
[CrossRef] [PubMed]

2008 (3)

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, “Multispectral confocal microendoscope for in vivo and in situ imaging,” J. Biomed. Opt.13(4), 044016–044019 (2008).
[CrossRef] [PubMed]

N. Bozinovic, C. Ventalon, T. Ford, and J. Mertz, “Fluorescence endomicroscopy with structured illumination,” Opt. Express16(11), 8016–8025 (2008).
[CrossRef] [PubMed]

2007 (4)

2006 (4)

L. Fu, A. Jain, H. K. Xie, C. Cranfield, and M. Gu, “Nonlinear optical endoscopy based on a double-clad photonic crystal fiber and a MEMS mirror,” Opt. Express14(3), 1027–1032 (2006).
[CrossRef] [PubMed]

R. K. Y. Chan, P. K. Lim, X. Z. Wang, and M. H. Chan, “Fourier transform ultraviolet-visible spectrometer based on a beam-folding technique,” Opt. Lett.31(7), 903–905 (2006).
[CrossRef] [PubMed]

Y. Garini, I. T. Young, and G. McNamara, “Spectral imaging: principles and applications,” Cytometry A69A(8), 735–747 (2006).
[CrossRef]

L. L. Nuffer, P. A. Medvick, H. P. Foote, and J. C. Solinsky, “Multispectral/hyperspectral image enhancement for biological cell analysis,” Cytometry A69(8), 897–903 (2006).
[CrossRef] [PubMed]

2005 (3)

E. E. Benarroch, “Neuron-astrocyte interactions: partnership for normal function and disease in the central nervous system,” Mayo Clin. Proc.80(10), 1326–1338 (2005).
[CrossRef] [PubMed]

J. T. Motz, D. Yelin, B. J. Vakoc, B. E. Bouma, and G. J. Tearney, “Spectral- and frequency-encoded fluorescence imaging,” Opt. Lett.30(20), 2760–2762 (2005).
[CrossRef] [PubMed]

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

2004 (1)

A. Nimmerjahn, F. Kirchhoff, J. N. D. Kerr, and F. Helmchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods1(1), 31–37 (2004).
[CrossRef] [PubMed]

2003 (3)

C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth, “In vivo two-photon calcium imaging of neuronal networks,” Proc. Natl. Acad. Sci. U.S.A.100(12), 7319–7324 (2003).
[CrossRef] [PubMed]

T. Zimmermann, J. Rietdorf, and R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett.546(1), 87–92 (2003).
[CrossRef] [PubMed]

J. Siegel, D. S. Elson, S. E. D. Webb, K. C. B. Lee, A. Vlandas, G. L. Gambaruto, S. Lévêque-Fort, M. J. Lever, P. J. Tadrous, G. W. H. Stamp, A. L. Wallace, A. Sandison, T. F. Watson, F. Alvarez, and P. M. W. French, “Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles,” Appl. Opt.42(16), 2995–3004 (2003).
[CrossRef] [PubMed]

2002 (2)

R. K. Jain, L. L. Munn, and D. Fukumura, “Dissecting tumor pathophysiology using intravital microscopy,” Nat. Rev. Cancer2(4), 266–276 (2002).
[CrossRef] [PubMed]

V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc.207(Pt 2), 108–117 (2002).
[CrossRef] [PubMed]

2000 (1)

1993 (2)

Abramowski, D.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Adelsberger, H.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Alvarez, F.

Ameer-Beg, S.

Avendaño, C.

C. Porrero, P. Rubio-Garrido, C. Avendaño, and F. Clascá, “Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice,” Brain Res.1345, 59–72 (2010).
[CrossRef] [PubMed]

Aziz, D.

Bartoo, A. C.

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Bedard, N.

R. T. Kester, N. Bedard, L. Gao, and T. S. Tkaczyk, “Real-time snapshot hyperspectral imaging endoscope,” J. Biomed. Opt.16(5), 056005 (2011).
[CrossRef] [PubMed]

Benarroch, E. E.

E. E. Benarroch, “Neuron-astrocyte interactions: partnership for normal function and disease in the central nervous system,” Mayo Clin. Proc.80(10), 1326–1338 (2005).
[CrossRef] [PubMed]

Bouma, B. E.

Bourg-Heckly, G.

Bozinovic, N.

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

N. Bozinovic, C. Ventalon, T. Ford, and J. Mertz, “Fluorescence endomicroscopy with structured illumination,” Opt. Express16(11), 8016–8025 (2008).
[CrossRef] [PubMed]

Burns, L. D.

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

Busche, M. A.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Chan, M. H.

Chan, R. K. Y.

Cheung, E. L. M.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Chu, K. K.

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Chuang, F. S.

Ciurea, T.

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

Clascá, F.

C. Porrero, P. Rubio-Garrido, C. Avendaño, and F. Clascá, “Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice,” Brain Res.1345, 59–72 (2010).
[CrossRef] [PubMed]

Cocker, E. D.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Cook, R.

Cranfield, C.

De Palma, G. D.

G. D. De Palma, “Confocal laser endomicroscopy in the “in vivo” histological diagnosis of the gastrointestinal tract,” World J. Gastroenterol.15(46), 5770–5775 (2009).
[CrossRef] [PubMed]

Dubaj, V.

V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc.207(Pt 2), 108–117 (2002).
[CrossRef] [PubMed]

Eichhoff, G.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Elson, D. S.

Engelbrecht, C. J.

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

C. J. Engelbrecht, F. Voigt, and F. Helmchen, “Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging,” Opt. Lett.35(9), 1413–1415 (2010).
[CrossRef] [PubMed]

Farwell, D. G.

Festy, F.

Flusberg, B. A.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Foote, H. P.

L. L. Nuffer, P. A. Medvick, H. P. Foote, and J. C. Solinsky, “Multispectral/hyperspectral image enhancement for biological cell analysis,” Cytometry A69(8), 897–903 (2006).
[CrossRef] [PubMed]

Ford, T.

Ford, T. N.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105–021107 (2012).
[CrossRef] [PubMed]

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

French, P. M. W.

Fruhwirth, G. O.

Fu, L.

Fukumura, D.

R. K. Jain, L. L. Munn, and D. Fukumura, “Dissecting tumor pathophysiology using intravital microscopy,” Nat. Rev. Cancer2(4), 266–276 (2002).
[CrossRef] [PubMed]

Gambaruto, G. L.

Gao, L.

N. Hagen, R. T. Kester, L. Gao, and T. S. Tkaczyk, “Snapshot advantage: a review of the light collection improvement for parallel high-dimensional measurement systems,” Opt. Eng.51(11), 111702 (2012).
[CrossRef] [PubMed]

R. T. Kester, N. Bedard, L. Gao, and T. S. Tkaczyk, “Real-time snapshot hyperspectral imaging endoscope,” J. Biomed. Opt.16(5), 056005 (2011).
[CrossRef] [PubMed]

Gao, L. A.

Garaschuk, O.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth, “In vivo two-photon calcium imaging of neuronal networks,” Proc. Natl. Acad. Sci. U.S.A.100(12), 7319–7324 (2003).
[CrossRef] [PubMed]

Garini, Y.

Y. Garini, I. T. Young, and G. McNamara, “Spectral imaging: principles and applications,” Cytometry A69A(8), 735–747 (2006).
[CrossRef]

Georgescu, C. V.

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

Gheonea, D. I.

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

Ghosh, K. K.

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

Gillenwater, A.

Giniunas, L.

Gmitro, A. F.

Gu, M.

Haass, C.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Hagen, N.

N. Hagen, R. T. Kester, L. Gao, and T. S. Tkaczyk, “Snapshot advantage: a review of the light collection improvement for parallel high-dimensional measurement systems,” Opt. Eng.51(11), 111702 (2012).
[CrossRef] [PubMed]

L. A. Gao, R. T. Kester, N. Hagen, and T. S. Tkaczyk, “Snapshot image mapping spectrometer (IMS) with high sampling density for hyperspectral microscopy,” Opt. Express18(14), 14330–14344 (2010).
[CrossRef] [PubMed]

Harris, M.

V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc.207(Pt 2), 108–117 (2002).
[CrossRef] [PubMed]

Helmchen, F.

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

C. J. Engelbrecht, F. Voigt, and F. Helmchen, “Miniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging,” Opt. Lett.35(9), 1413–1415 (2010).
[CrossRef] [PubMed]

A. Nimmerjahn, F. Kirchhoff, J. N. D. Kerr, and F. Helmchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods1(1), 31–37 (2004).
[CrossRef] [PubMed]

Ho, E. T. W.

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

Holthoff, K.

C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth, “In vivo two-photon calcium imaging of neuronal networks,” Proc. Natl. Acad. Sci. U.S.A.100(12), 7319–7324 (2003).
[CrossRef] [PubMed]

Hourtoule, C.

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Jain, A.

Jain, R. K.

R. K. Jain, L. L. Munn, and D. Fukumura, “Dissecting tumor pathophysiology using intravital microscopy,” Nat. Rev. Cancer2(4), 266–276 (2002).
[CrossRef] [PubMed]

Jean, F.

Jo, J. A.

D. S. Elson, J. A. Jo, and L. Marcu, “Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens,” New J. Phys.9(5), 127 (2007).
[CrossRef] [PubMed]

Jung, J. C.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Juskaitis, R.

Kerr, J. N. D.

A. Nimmerjahn, F. Kirchhoff, J. N. D. Kerr, and F. Helmchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods1(1), 31–37 (2004).
[CrossRef] [PubMed]

Kester, R. T.

N. Hagen, R. T. Kester, L. Gao, and T. S. Tkaczyk, “Snapshot advantage: a review of the light collection improvement for parallel high-dimensional measurement systems,” Opt. Eng.51(11), 111702 (2012).
[CrossRef] [PubMed]

R. T. Kester, N. Bedard, L. Gao, and T. S. Tkaczyk, “Real-time snapshot hyperspectral imaging endoscope,” J. Biomed. Opt.16(5), 056005 (2011).
[CrossRef] [PubMed]

L. A. Gao, R. T. Kester, N. Hagen, and T. S. Tkaczyk, “Snapshot image mapping spectrometer (IMS) with high sampling density for hyperspectral microscopy,” Opt. Express18(14), 14330–14344 (2010).
[CrossRef] [PubMed]

Kirchhoff, F.

A. Nimmerjahn, F. Kirchhoff, J. N. D. Kerr, and F. Helmchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods1(1), 31–37 (2004).
[CrossRef] [PubMed]

Konnerth, A.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth, “In vivo two-photon calcium imaging of neuronal networks,” Proc. Natl. Acad. Sci. U.S.A.100(12), 7319–7324 (2003).
[CrossRef] [PubMed]

Lee, C. M.

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

Lee, K. C. B.

Lévêque-Fort, S.

Lever, M. J.

Lim, D.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105–021107 (2012).
[CrossRef] [PubMed]

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Lim, P. K.

Makhlouf, H.

H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, “Multispectral confocal microendoscope for in vivo and in situ imaging,” J. Biomed. Opt.13(4), 044016–044019 (2008).
[CrossRef] [PubMed]

Malos, A.

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

Marcu, L.

Y. Sun, J. Phipps, D. S. Elson, H. Stoy, S. Tinling, J. Meier, B. Poirier, F. S. Chuang, D. G. Farwell, and L. Marcu, “Fluorescence lifetime imaging microscopy: in vivo application to diagnosis of oral carcinoma,” Opt. Lett.34(13), 2081–2083 (2009).
[CrossRef] [PubMed]

D. S. Elson, J. A. Jo, and L. Marcu, “Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens,” New J. Phys.9(5), 127 (2007).
[CrossRef] [PubMed]

Mazzolini, A.

V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc.207(Pt 2), 108–117 (2002).
[CrossRef] [PubMed]

McNamara, G.

Y. Garini, I. T. Young, and G. McNamara, “Spectral imaging: principles and applications,” Cytometry A69A(8), 735–747 (2006).
[CrossRef]

Medvick, P. A.

L. L. Nuffer, P. A. Medvick, H. P. Foote, and J. C. Solinsky, “Multispectral/hyperspectral image enhancement for biological cell analysis,” Cytometry A69(8), 897–903 (2006).
[CrossRef] [PubMed]

Meier, J.

Mertz, J.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105–021107 (2012).
[CrossRef] [PubMed]

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

N. Bozinovic, C. Ventalon, T. Ford, and J. Mertz, “Fluorescence endomicroscopy with structured illumination,” Opt. Express16(11), 8016–8025 (2008).
[CrossRef] [PubMed]

Motz, J. T.

Mukamel, E. A.

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

Muldoon, T. J.

Munn, L. L.

R. K. Jain, L. L. Munn, and D. Fukumura, “Dissecting tumor pathophysiology using intravital microscopy,” Nat. Rev. Cancer2(4), 266–276 (2002).
[CrossRef] [PubMed]

Ng, T.

Nida, D. L.

Nimmerjahn, A.

A. Nimmerjahn, F. Kirchhoff, J. N. D. Kerr, and F. Helmchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods1(1), 31–37 (2004).
[CrossRef] [PubMed]

Nuffer, L. L.

L. L. Nuffer, P. A. Medvick, H. P. Foote, and J. C. Solinsky, “Multispectral/hyperspectral image enhancement for biological cell analysis,” Cytometry A69(8), 897–903 (2006).
[CrossRef] [PubMed]

Peng, L.

Pepperkok, R.

T. Zimmermann, J. Rietdorf, and R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett.546(1), 87–92 (2003).
[CrossRef] [PubMed]

Phipps, J.

Pierce, M. C.

Piyawattanametha, W.

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Poirier, B.

Popescu, C.

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

Porrero, C.

C. Porrero, P. Rubio-Garrido, C. Avendaño, and F. Clascá, “Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice,” Brain Res.1345, 59–72 (2010).
[CrossRef] [PubMed]

Redmond, R. W.

Richards-Kortum, R.

Rietdorf, J.

T. Zimmermann, J. Rietdorf, and R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett.546(1), 87–92 (2003).
[CrossRef] [PubMed]

Rouse, A. R.

H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, “Multispectral confocal microendoscope for in vivo and in situ imaging,” J. Biomed. Opt.13(4), 044016–044019 (2008).
[CrossRef] [PubMed]

A. R. Rouse and A. F. Gmitro, “Multispectral imaging with a confocal microendoscope,” Opt. Lett.25(23), 1708–1710 (2000).
[CrossRef] [PubMed]

Rubio-Garrido, P.

C. Porrero, P. Rubio-Garrido, C. Avendaño, and F. Clascá, “Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice,” Brain Res.1345, 59–72 (2010).
[CrossRef] [PubMed]

Saftoiu, A.

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

Sandison, A.

Santos, S.

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Schnitzer, M. J.

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Seibel, E. J.

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

Shatalin, S. V.

Siegel, J.

Singh, S. K.

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

Solinsky, J. C.

L. L. Nuffer, P. A. Medvick, H. P. Foote, and J. C. Solinsky, “Multispectral/hyperspectral image enhancement for biological cell analysis,” Cytometry A69(8), 897–903 (2006).
[CrossRef] [PubMed]

Soper, T. D.

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

Stamp, G. W. H.

Staufenbiel, M.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Stosiek, C.

C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth, “In vivo two-photon calcium imaging of neuronal networks,” Proc. Natl. Acad. Sci. U.S.A.100(12), 7319–7324 (2003).
[CrossRef] [PubMed]

Stoy, H.

Sun, Y.

Tadrous, P. J.

Tanbakuchi, A. A.

H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, “Multispectral confocal microendoscope for in vivo and in situ imaging,” J. Biomed. Opt.13(4), 044016–044019 (2008).
[CrossRef] [PubMed]

Tearney, G. J.

Tinling, S.

Tkaczyk, T. S.

N. Hagen, R. T. Kester, L. Gao, and T. S. Tkaczyk, “Snapshot advantage: a review of the light collection improvement for parallel high-dimensional measurement systems,” Opt. Eng.51(11), 111702 (2012).
[CrossRef] [PubMed]

R. T. Kester, N. Bedard, L. Gao, and T. S. Tkaczyk, “Real-time snapshot hyperspectral imaging endoscope,” J. Biomed. Opt.16(5), 056005 (2011).
[CrossRef] [PubMed]

L. A. Gao, R. T. Kester, N. Hagen, and T. S. Tkaczyk, “Snapshot image mapping spectrometer (IMS) with high sampling density for hyperspectral microscopy,” Opt. Express18(14), 14330–14344 (2010).
[CrossRef] [PubMed]

Udovich, J. A.

H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, “Multispectral confocal microendoscope for in vivo and in situ imaging,” J. Biomed. Opt.13(4), 044016–044019 (2008).
[CrossRef] [PubMed]

Vakoc, B. J.

Ventalon, C.

Viellerobe, B.

Vlandas, A.

Voigt, F.

Wallace, A. L.

Wang, X. Z.

Watson, T.

Watson, T. F.

Webb, S. E. D.

Wiederhold, K.-H.

M. A. Busche, G. Eichhoff, H. Adelsberger, D. Abramowski, K.-H. Wiederhold, C. Haass, M. Staufenbiel, A. Konnerth, and O. Garaschuk, “Clusters of hyperactive neurons near amyloid plaques in a mouse model of Alzheimer’s disease,” Science321(5896), 1686–1689 (2008).
[CrossRef] [PubMed]

Williams, M. D.

Wilt, B. A.

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

Wood, A.

V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc.207(Pt 2), 108–117 (2002).
[CrossRef] [PubMed]

Xie, H. K.

Yelin, D.

Young, I. T.

Y. Garini, I. T. Young, and G. McNamara, “Spectral imaging: principles and applications,” Cytometry A69A(8), 735–747 (2006).
[CrossRef]

Zimmermann, T.

T. Zimmermann, J. Rietdorf, and R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett.546(1), 87–92 (2003).
[CrossRef] [PubMed]

Annu. Rev. Neurosci. (1)

B. A. Wilt, L. D. Burns, E. T. W. Ho, K. K. Ghosh, E. A. Mukamel, and M. J. Schnitzer, “Advances in light microscopy for neuroscience,” Annu. Rev. Neurosci.32(1), 435–506 (2009).
[CrossRef] [PubMed]

Appl. Opt. (2)

Brain Res. (1)

C. Porrero, P. Rubio-Garrido, C. Avendaño, and F. Clascá, “Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice,” Brain Res.1345, 59–72 (2010).
[CrossRef] [PubMed]

Cytometry A (2)

Y. Garini, I. T. Young, and G. McNamara, “Spectral imaging: principles and applications,” Cytometry A69A(8), 735–747 (2006).
[CrossRef]

L. L. Nuffer, P. A. Medvick, H. P. Foote, and J. C. Solinsky, “Multispectral/hyperspectral image enhancement for biological cell analysis,” Cytometry A69(8), 897–903 (2006).
[CrossRef] [PubMed]

FEBS Lett. (1)

T. Zimmermann, J. Rietdorf, and R. Pepperkok, “Spectral imaging and its applications in live cell microscopy,” FEBS Lett.546(1), 87–92 (2003).
[CrossRef] [PubMed]

J. Biomed. Opt. (4)

R. T. Kester, N. Bedard, L. Gao, and T. S. Tkaczyk, “Real-time snapshot hyperspectral imaging endoscope,” J. Biomed. Opt.16(5), 056005 (2011).
[CrossRef] [PubMed]

S. Santos, K. K. Chu, D. Lim, N. Bozinovic, T. N. Ford, C. Hourtoule, A. C. Bartoo, S. K. Singh, and J. Mertz, “Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle,” J. Biomed. Opt.14(3), 030502–030503 (2009).
[CrossRef] [PubMed]

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt.17(2), 021105–021107 (2012).
[CrossRef] [PubMed]

H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, “Multispectral confocal microendoscope for in vivo and in situ imaging,” J. Biomed. Opt.13(4), 044016–044019 (2008).
[CrossRef] [PubMed]

J. Biophotonics (1)

C. M. Lee, C. J. Engelbrecht, T. D. Soper, F. Helmchen, and E. J. Seibel, “Scanning fiber endoscopy with highly flexible, 1 mm catheter scopes for wide-field, full-color imaging,” J. Biophotonics3(5-6), 385–407 (2010).
[CrossRef] [PubMed]

J. Gastrointestin. Liver Dis. (1)

D. I. Gheonea, A. Saftoiu, T. Ciurea, C. Popescu, C. V. Georgescu, and A. Malos, “Confocal laser endomicroscopy of the colon,” J. Gastrointestin. Liver Dis.19(2), 207–211 (2010).
[PubMed]

J. Microsc. (1)

V. Dubaj, A. Mazzolini, A. Wood, and M. Harris, “Optic fiber bundle contact imaging probe employing a laser scanning confocal microscope,” J. Microsc.207(Pt 2), 108–117 (2002).
[CrossRef] [PubMed]

Mayo Clin. Proc. (1)

E. E. Benarroch, “Neuron-astrocyte interactions: partnership for normal function and disease in the central nervous system,” Mayo Clin. Proc.80(10), 1326–1338 (2005).
[CrossRef] [PubMed]

Nat. Methods (2)

A. Nimmerjahn, F. Kirchhoff, J. N. D. Kerr, and F. Helmchen, “Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo,” Nat. Methods1(1), 31–37 (2004).
[CrossRef] [PubMed]

B. A. Flusberg, E. D. Cocker, W. Piyawattanametha, J. C. Jung, E. L. M. Cheung, and M. J. Schnitzer, “Fiber-optic fluorescence imaging,” Nat. Methods2(12), 941–950 (2005).
[CrossRef] [PubMed]

Nat. Rev. Cancer (1)

R. K. Jain, L. L. Munn, and D. Fukumura, “Dissecting tumor pathophysiology using intravital microscopy,” Nat. Rev. Cancer2(4), 266–276 (2002).
[CrossRef] [PubMed]

New J. Phys. (1)

D. S. Elson, J. A. Jo, and L. Marcu, “Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens,” New J. Phys.9(5), 127 (2007).
[CrossRef] [PubMed]

Opt. Eng. (1)

N. Hagen, R. T. Kester, L. Gao, and T. S. Tkaczyk, “Snapshot advantage: a review of the light collection improvement for parallel high-dimensional measurement systems,” Opt. Eng.51(11), 111702 (2012).
[CrossRef] [PubMed]

Opt. Express (6)

Opt. Lett. (7)

Proc. Natl. Acad. Sci. U.S.A. (1)

C. Stosiek, O. Garaschuk, K. Holthoff, and A. Konnerth, “In vivo two-photon calcium imaging of neuronal networks,” Proc. Natl. Acad. Sci. U.S.A.100(12), 7319–7324 (2003).
[CrossRef] [PubMed]

Science (1)

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G. D. De Palma, “Confocal laser endomicroscopy in the “in vivo” histological diagnosis of the gastrointestinal tract,” World J. Gastroenterol.15(46), 5770–5775 (2009).
[CrossRef] [PubMed]

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S. Wartewig, IR and Raman Spectroscopy (Wiley, 2003).

A. R. Rouse, “Multi-spectral confocal microendoscope for in-vivo imaging,” Ph.D Thesis, University of Arizona (2004).

J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, 2006).

J. Yin, Y. Shao, J. Qu, H. Lin, and H. Niu, “A fluorescence sectioning endoscopy using dynamic speckle illumination,” in Endoscopic Microscopy III, (SPIE, 2008), 68510N.

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Figures (8)

Fig. 1
Fig. 1

Imaging Fourier transform endospectroscopy (IFTES). DM: dichroic mirror; M: mirror; Obj: Objective lens; LP: long pass filter.

Fig. 2
Fig. 2

(a) Image of the USAF target used to determine spatial resolution illuminated by a cold light source from the rear side. (b) Enlarged detail of the red rectangle in (a). Scale bar in (a) is 100 μm.

Fig. 3
Fig. 3

Images from excised normal mouse tissues: (a) liver, (b) pancreas, (c) kidney, and (d) peritoneal wall. Scale bar is 100 μm.

Fig. 4
Fig. 4

Spectral imaging of a He-Ne Laser. (a) Interferogram frame of the fiber bundle illuminated by a He-Ne laser (false color by ImageJ software; the same below). (b) Enlarged detail of the blue rectangle in (a). (c) Spectrum of the red pixel pointed out by the blue arrow in (b). Scale bar is 100 μm.

Fig. 5
Fig. 5

(a) Data acquisition time T using various spectral resolution ∆λ (blue line). Red circles represent corresponding T by ∆λ of 21.8 nm, 10.9 nm, 2.2 nm, and 0.2 nm at 521 nm in wavelength measured by IFTES system, respectively. (b)–(e) Corresponding normalized emission spectra of fluorescein sodium solution with spectral resolution ∆λ of 21.8 nm, 10.9 nm, 2.2 nm and 0.2 nm at 521 nm in wavelength measured by IFTES system (blue lines) and commercial spectroscopy (red lines), respectively.

Fig. 6
Fig. 6

Spectral imaging results from C57 mouse skin in situ. (a) Full FOV image acquired by the fiber bundle in the living C57 mouse. Red rectangle represents ROI range. Scale bar is 50 μm. (b) Emission spectra of the bright spot pointed out by the red cross in (a) by the IFTES system and commercial spectroscopy are plotted as blue and red lines, respectively.

Fig. 7
Fig. 7

Spectral imaging from a mixture of 10-14 μm dual-color fluorescent beads. (a) Interferogram frame acquired by IFTES. (b) Spectral images at wavelength from 501 nm to 690 nm. (c) Spectra associated with yellow (green line) and purple (red line) beads measured by IFTES, respectively. (d) False-color composite image of the mixture. Green and red represent yellow and purple beads, respectively. Scale bars are 10 μm.

Fig. 8
Fig. 8

Spectral imaging from YFP-transgenic mouse labeled with SR101: (a) Photograph of YFP-transgenic mouse with inserting fiber bundle into cerebral cortex. (b) Fluorescence image of mouse brain in full FOV acquired by IFTES. (c) False-color composite image of ROI enclosed by the red rectangle in (b). Green and red represent neuron and astrocyte, respectively. (d) Emission spectra associated with neuron (green line) and astrocyte (red line) measured by IFTES, respectively. Scale bars in (b) and (c) are 50 μm and 10 μm, respectively.

Equations (3)

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T= d Δdfr .
d 1.207 λ 2 2Δλ ,
T 1.207 λ 2 ΔdΔλfr .

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