Abstract

We present a new technique for the correction of optical aberrations in wide-field fluorescence microscopy. Segmented-Pupil Image Interferometry (SPII) uses a liquid crystal spatial light modulator placed in the microscope’s pupil plane to split the wavefront originating from a fluorescent object into an array of individual beams. Distortion of the wavefront arising from either system or sample aberrations results in displacement of the images formed from the individual pupil segments. Analysis of image registration allows for the local tilt in the wavefront at each segment to be corrected with respect to a central reference. A second correction step optimizes the image intensity by adjusting the relative phase of each pupil segment through image interferometry. This ensures that constructive interference between all segments is achieved at the image plane. Improvements in image quality are observed when Segmented-Pupil Image Interferometry is applied to correct aberrations arising from the microscope’s optical path.

© 2012 OSA

PDF Article

References

  • View by:
  • |
  • |
  • |

  1. M. J. Booth, “Adaptive optics in microscopy,” Philos. Transact. A Math. Phys. Eng. Sci. 365(1861), 2829–2843 (2007).
    [CrossRef] [PubMed]
  2. J. Sebag, J. Arnaud, G. Lelièvre, J. L. Nieto, and E. L. Coarer, “High-resolution imaging using pupil segmentation,” J. Opt. Soc. Am. A 7(7), 1237–1242 (1990).
    [CrossRef]
  3. G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).
  4. J. Liang, B. Grimm, S. Goelz, and J. F. Bille, “Objective measurement of wave aberrations of the human eye with the use of a Hartmann-Shack wave-front sensor,” J. Opt. Soc. Am. A 11(7), 1949–1957 (1994).
    [CrossRef] [PubMed]
  5. P. Prieto, E. Fernández, S. Manzanera, and P. Artal, “Adaptive optics with a programmable phase modulator: applications in the human eye,” Opt. Express 12(17), 4059–4071 (2004).
    [CrossRef] [PubMed]
  6. E. Fernandez and P. Artal, “Membrane deformable mirror for adaptive optics: performance limits in visual optics,” Opt. Express 11(9), 1056–1069 (2003).
    [CrossRef] [PubMed]
  7. J. W. Hardy, Adaptive Optics for Astronomical Telescopes (Oxford University Press, New York, 1988).
  8. J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol. 20(1), 106–110 (2009).
    [CrossRef] [PubMed]
  9. W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
    [CrossRef] [PubMed]
  10. P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
    [CrossRef] [PubMed]
  11. I. M. Vellekoop and A. P. Mosk, “Focusing coherent light through opaque strongly scattering media,” Opt. Lett. 32(16), 2309–2311 (2007).
    [CrossRef] [PubMed]
  12. N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7(2), 141–147 (2010).
    [CrossRef] [PubMed]
  13. T. Cizmar, M. Mazilu, and K. Dholakia, “In situ wavefront correction and its application to micromanipulation,” Nat. Photonics 4(6), 388–394 (2010).
    [CrossRef]
  14. R. W. Bowman, A. J. Wright, and M. J. Padgett, “An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers,” J. Opt. 12(12), 124004 (2010).
    [CrossRef]
  15. G. Hall, G. C. Spalding, P. J. Campagnola, J. G. White, and K. W. Eliceiri, “Fast localized wavefront correction using area-mapped phase-shift interferometry,” Opt. Lett. 36(15), 2892–2894 (2011).
    [CrossRef] [PubMed]
  16. D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
    [CrossRef] [PubMed]
  17. P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
    [CrossRef] [PubMed]
  18. E. Fuchs, J. Jaffe, R. Long, and F. Azam, “Thin laser light sheet microscope for microbial oceanography,” Opt. Express 10(2), 145–154 (2002).
    [PubMed]

2011 (2)

W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
[CrossRef] [PubMed]

G. Hall, G. C. Spalding, P. J. Campagnola, J. G. White, and K. W. Eliceiri, “Fast localized wavefront correction using area-mapped phase-shift interferometry,” Opt. Lett. 36(15), 2892–2894 (2011).
[CrossRef] [PubMed]

2010 (4)

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7(2), 141–147 (2010).
[CrossRef] [PubMed]

T. Cizmar, M. Mazilu, and K. Dholakia, “In situ wavefront correction and its application to micromanipulation,” Nat. Photonics 4(6), 388–394 (2010).
[CrossRef]

R. W. Bowman, A. J. Wright, and M. J. Padgett, “An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers,” J. Opt. 12(12), 124004 (2010).
[CrossRef]

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

2009 (1)

J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol. 20(1), 106–110 (2009).
[CrossRef] [PubMed]

2008 (1)

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[CrossRef] [PubMed]

2007 (2)

I. M. Vellekoop and A. P. Mosk, “Focusing coherent light through opaque strongly scattering media,” Opt. Lett. 32(16), 2309–2311 (2007).
[CrossRef] [PubMed]

M. J. Booth, “Adaptive optics in microscopy,” Philos. Transact. A Math. Phys. Eng. Sci. 365(1861), 2829–2843 (2007).
[CrossRef] [PubMed]

2005 (1)

2004 (1)

2003 (1)

2002 (1)

1994 (1)

1990 (1)

1988 (1)

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Arnaud, J.

Artal, P.

Azam, F.

Beaurepaire, E.

W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
[CrossRef] [PubMed]

Betzig, E.

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7(2), 141–147 (2010).
[CrossRef] [PubMed]

Bille, J. F.

Booth, M. J.

M. J. Booth, “Adaptive optics in microscopy,” Philos. Transact. A Math. Phys. Eng. Sci. 365(1861), 2829–2843 (2007).
[CrossRef] [PubMed]

Bowman, R. W.

R. W. Bowman, A. J. Wright, and M. J. Padgett, “An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers,” J. Opt. 12(12), 124004 (2010).
[CrossRef]

Campagnola, P. J.

Carroll, J.

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

Cizmar, T.

T. Cizmar, M. Mazilu, and K. Dholakia, “In situ wavefront correction and its application to micromanipulation,” Nat. Photonics 4(6), 388–394 (2010).
[CrossRef]

Coarer, E. L.

Débarre, D.

W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
[CrossRef] [PubMed]

Dholakia, K.

T. Cizmar, M. Mazilu, and K. Dholakia, “In situ wavefront correction and its application to micromanipulation,” Nat. Photonics 4(6), 388–394 (2010).
[CrossRef]

Dubis, A. M.

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

Duncan, J. L.

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

Eliceiri, K. W.

Fernandez, E.

Fernández, E.

Fuchs, E.

Girkin, J. M.

J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol. 20(1), 106–110 (2009).
[CrossRef] [PubMed]

Godara, P.

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

Goelz, S.

Grimm, B.

Hall, G.

Jaffe, J.

Ji, N.

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7(2), 141–147 (2010).
[CrossRef] [PubMed]

Keller, P. J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[CrossRef] [PubMed]

Lelievre, G.

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Lelièvre, G.

Liang, J.

Llebaria, A.

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Long, R.

Manzanera, S.

Mazilu, M.

T. Cizmar, M. Mazilu, and K. Dholakia, “In situ wavefront correction and its application to micromanipulation,” Nat. Photonics 4(6), 388–394 (2010).
[CrossRef]

Milkie, D. E.

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7(2), 141–147 (2010).
[CrossRef] [PubMed]

Mosk, A. P.

Nieto, J. L.

Nieto, J.-L.

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Oron, D.

Padgett, M. J.

R. W. Bowman, A. J. Wright, and M. J. Padgett, “An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers,” J. Opt. 12(12), 124004 (2010).
[CrossRef]

Poland, S.

J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol. 20(1), 106–110 (2009).
[CrossRef] [PubMed]

Prieto, P.

Roorda, A.

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

Salmon, D.

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Schmidt, A. D.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[CrossRef] [PubMed]

Sebag, J.

Silberberg, Y.

Spalding, G. C.

Stelzer, E. H. K.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[CrossRef] [PubMed]

Supatto, W.

W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
[CrossRef] [PubMed]

Tal, E.

Thouvenot, E.

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Truong, T. V.

W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
[CrossRef] [PubMed]

Vellekoop, I. M.

White, J. G.

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[CrossRef] [PubMed]

Wright, A. J.

R. W. Bowman, A. J. Wright, and M. J. Padgett, “An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers,” J. Opt. 12(12), 124004 (2010).
[CrossRef]

J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol. 20(1), 106–110 (2009).
[CrossRef] [PubMed]

Astron. Astrophys. (1)

G. Lelievre, J.-L. Nieto, E. Thouvenot, D. Salmon, and A. Llebaria, “Very high resolution imaging using sub-pupil apertures, recentering and selection of short exposures,” Astron. Astrophys. 200, 301–311 (1988).

Curr. Opin. Biotechnol. (1)

J. M. Girkin, S. Poland, and A. J. Wright, “Adaptive optics for deeper imaging of biological samples,” Curr. Opin. Biotechnol. 20(1), 106–110 (2009).
[CrossRef] [PubMed]

Curr. Opin. Genet. Dev. (1)

W. Supatto, T. V. Truong, D. Débarre, and E. Beaurepaire, “Advances in multiphoton microscopy for imaging embryos,” Curr. Opin. Genet. Dev. 21(5), 538–548 (2011).
[CrossRef] [PubMed]

J. Opt. (1)

R. W. Bowman, A. J. Wright, and M. J. Padgett, “An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers,” J. Opt. 12(12), 124004 (2010).
[CrossRef]

J. Opt. Soc. Am. A (2)

Nat. Methods (1)

N. Ji, D. E. Milkie, and E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues,” Nat. Methods 7(2), 141–147 (2010).
[CrossRef] [PubMed]

Nat. Photonics (1)

T. Cizmar, M. Mazilu, and K. Dholakia, “In situ wavefront correction and its application to micromanipulation,” Nat. Photonics 4(6), 388–394 (2010).
[CrossRef]

Opt. Express (4)

Opt. Lett. (2)

Optom. Vis. Sci. (1)

P. Godara, A. M. Dubis, A. Roorda, J. L. Duncan, and J. Carroll, “Adaptive optics retinal imaging: emerging clinical applications,” Optom. Vis. Sci. 87(12), 930–941 (2010).
[CrossRef] [PubMed]

Philos. Transact. A Math. Phys. Eng. Sci. (1)

M. J. Booth, “Adaptive optics in microscopy,” Philos. Transact. A Math. Phys. Eng. Sci. 365(1861), 2829–2843 (2007).
[CrossRef] [PubMed]

Science (1)

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[CrossRef] [PubMed]

Other (1)

J. W. Hardy, Adaptive Optics for Astronomical Telescopes (Oxford University Press, New York, 1988).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Metrics