Abstract

In this paper, a concept of polarimetric total internal reflection (TIR) biosensor based on the method of temporal phase modulation is presented. Measurements of the phase difference between s- and p- polarized light combined with their amplitudes allow simultaneous detection of the bulk refractive index and thickness of the surface biofilms. Obtained experimental sensitivity is better than 10−5 in terms of refractive index unit and 0.5 nm in biolayer thickness. Relatively simple technological implementation of the TIR sensors on the base of inexpensive and transparent substrates opens a number of novel applications in biosensing and microscopy.

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    [CrossRef]
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    [CrossRef] [PubMed]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]
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    [CrossRef]

2009 (2)

O. Svensson and T. Arnebrant, “Adsorption of serum albumin on silica--the influence of surface cleaning procedures,” J. Colloid Interface Sci. 344(1), 44–47 (2009).
[CrossRef]

S. Patskovsky, I.-H. Song, M. Meunier, and A. V. Kabashin, “Silicon based total internal reflection bio and chemical sensing with spectral phase detection,” Opt. Express 17(23), 20847–20852 (2009).
[CrossRef] [PubMed]

2008 (2)

2007 (3)

S. Patskovsky, M. Meunier, and A. V. Kabashin, “Phase-sensitive silicon-based total internal reflection sensor,” Opt. Express 15(19 ), 12523–12528 (2007).
[CrossRef] [PubMed]

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

2006 (1)

P. Adam, J. Dostalek, and J. Homola, “Multiple surface plasmon spectroscopy for study of biomolecular systems,” Sens. Actuators B Chem. 113(2), 774–781 (2006).
[CrossRef]

2004 (1)

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

2002 (1)

M. D. Marazuela and M. C. Moreno-Bondi, “Fiber-optic biosensors--an overview,” Anal. Bioanal. Chem. 372(5-6), 664–682 (2002).
[CrossRef] [PubMed]

2000 (1)

1999 (1)

J. J. Cras, C. A. Rowe-Taitt, D. A. Nivens, and F. S. Ligler, “Comparison of chemical cleaning methods of glass in preparation for silanization,” Biosens. Bioelectron. 14(8-9), 683–688 (1999).
[CrossRef]

1996 (1)

K. A. Peterlinz and R. Georgiadis, “Two-color approach for determination of thickness and dielectric constant of thin films using surface plasmon resonance spectroscopy,” Opt. Commun. 130(4-6), 260–266 (1996).
[CrossRef]

1990 (1)

Adam, P.

P. Adam, J. Dostalek, and J. Homola, “Multiple surface plasmon spectroscopy for study of biomolecular systems,” Sens. Actuators B Chem. 113(2), 774–781 (2006).
[CrossRef]

Arnebrant, T.

O. Svensson and T. Arnebrant, “Adsorption of serum albumin on silica--the influence of surface cleaning procedures,” J. Colloid Interface Sci. 344(1), 44–47 (2009).
[CrossRef]

Badoz, J.

Baev, A.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Brandenburg, A.

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

Canit, J. C.

Chao, Y.-F.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Chen, S.-S.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Cras, J. J.

J. J. Cras, C. A. Rowe-Taitt, D. A. Nivens, and F. S. Ligler, “Comparison of chemical cleaning methods of glass in preparation for silanization,” Biosens. Bioelectron. 14(8-9), 683–688 (1999).
[CrossRef]

Dostalek, J.

P. Adam, J. Dostalek, and J. Homola, “Multiple surface plasmon spectroscopy for study of biomolecular systems,” Sens. Actuators B Chem. 113(2), 774–781 (2006).
[CrossRef]

Georgiadis, R.

K. A. Peterlinz and R. Georgiadis, “Two-color approach for determination of thickness and dielectric constant of thin films using surface plasmon resonance spectroscopy,” Opt. Commun. 130(4-6), 260–266 (1996).
[CrossRef]

Hastings, J. T.

Ho, H. P.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Hoffmann, C.

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

Homola, J.

P. Adam, J. Dostalek, and J. Homola, “Multiple surface plasmon spectroscopy for study of biomolecular systems,” Sens. Actuators B Chem. 113(2), 774–781 (2006).
[CrossRef]

Jing, G.

Kabashin, A. V.

Kasunic, K. J.

Keathley, P. D.

Law, W. C.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Leou, K.-C.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Ligler, F. S.

J. J. Cras, C. A. Rowe-Taitt, D. A. Nivens, and F. S. Ligler, “Comparison of chemical cleaning methods of glass in preparation for silanization,” Biosens. Bioelectron. 14(8-9), 683–688 (1999).
[CrossRef]

Lin, T.-L.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Liu, Y.-W.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Maisonneuve, M.

Marazuela, M. D.

M. D. Marazuela and M. C. Moreno-Bondi, “Fiber-optic biosensors--an overview,” Anal. Bioanal. Chem. 372(5-6), 664–682 (2002).
[CrossRef] [PubMed]

Markowicz, P.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Meunier, M.

Meyrueis, P.

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

Moreno-Bondi, M. C.

M. D. Marazuela and M. C. Moreno-Bondi, “Fiber-optic biosensors--an overview,” Anal. Bioanal. Chem. 372(5-6), 664–682 (2002).
[CrossRef] [PubMed]

Nivens, D. A.

J. J. Cras, C. A. Rowe-Taitt, D. A. Nivens, and F. S. Ligler, “Comparison of chemical cleaning methods of glass in preparation for silanization,” Biosens. Bioelectron. 14(8-9), 683–688 (1999).
[CrossRef]

Patskovsky, S.

Peterlinz, K. A.

K. A. Peterlinz and R. Georgiadis, “Two-color approach for determination of thickness and dielectric constant of thin films using surface plasmon resonance spectroscopy,” Opt. Commun. 130(4-6), 260–266 (1996).
[CrossRef]

Prasad, P. N.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Rowe-Taitt, C. A.

J. J. Cras, C. A. Rowe-Taitt, D. A. Nivens, and F. S. Ligler, “Comparison of chemical cleaning methods of glass in preparation for silanization,” Biosens. Bioelectron. 14(8-9), 683–688 (1999).
[CrossRef]

Roy, I.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Schirmer, B.

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

Schmitt, K.

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

Silverman, M. P.

Song, I.-H.

Svensson, O.

O. Svensson and T. Arnebrant, “Adsorption of serum albumin on silica--the influence of surface cleaning procedures,” J. Colloid Interface Sci. 344(1), 44–47 (2009).
[CrossRef]

Tsai, F.-H.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Wang, M.-W.

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Yong, K. T.

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

Anal. Bioanal. Chem. (1)

M. D. Marazuela and M. C. Moreno-Bondi, “Fiber-optic biosensors--an overview,” Anal. Bioanal. Chem. 372(5-6), 664–682 (2002).
[CrossRef] [PubMed]

Appl. Opt. (1)

Biosens. Bioelectron. (3)

J. J. Cras, C. A. Rowe-Taitt, D. A. Nivens, and F. S. Ligler, “Comparison of chemical cleaning methods of glass in preparation for silanization,” Biosens. Bioelectron. 14(8-9), 683–688 (1999).
[CrossRef]

K. Schmitt, B. Schirmer, C. Hoffmann, A. Brandenburg, and P. Meyrueis, “Interferometric biosensor based on planar optical waveguide sensor chips for label-free detection of surface bound bioreactions,” Biosens. Bioelectron. 22(11), 2591–2597 (2007).
[CrossRef]

W. C. Law, P. Markowicz, K. T. Yong, I. Roy, A. Baev, S. Patskovsky, A. V. Kabashin, H. P. Ho, and P. N. Prasad, “Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics,” Biosens. Bioelectron. 23(5), 627–632 (2007).
[CrossRef] [PubMed]

J. Colloid Interface Sci. (1)

O. Svensson and T. Arnebrant, “Adsorption of serum albumin on silica--the influence of surface cleaning procedures,” J. Colloid Interface Sci. 344(1), 44–47 (2009).
[CrossRef]

J. Opt. Soc. Am. A (1)

Jpn. J. Appl. Phys. (1)

M.-W. Wang, Y.-F. Chao, K.-C. Leou, F.-H. Tsai, T.-L. Lin, S.-S. Chen, and Y.-W. Liu, “Calibrations of Phase Modulation Amplitude of Photoelastic Modulator,” Jpn. J. Appl. Phys. 43(2), 827–832 (2004).
[CrossRef]

Opt. Commun. (1)

K. A. Peterlinz and R. Georgiadis, “Two-color approach for determination of thickness and dielectric constant of thin films using surface plasmon resonance spectroscopy,” Opt. Commun. 130(4-6), 260–266 (1996).
[CrossRef]

Opt. Express (3)

Opt. Lett. (1)

Sens. Actuators B Chem. (1)

P. Adam, J. Dostalek, and J. Homola, “Multiple surface plasmon spectroscopy for study of biomolecular systems,” Sens. Actuators B Chem. 113(2), 774–781 (2006).
[CrossRef]

Other (4)

E. Hecht, “The propagation of light,” in Optics, 4th ed. (Addison Wesley, 2002), pp. 86–148.

“Concentrative properties of aqueous solutions: density, refractive index, freezing point depression and viscosity,” in CRC Handbook of Chemistry and Physics, Internet Version 2005, D. R. Lide, ed. (CRC press, Boca Raton, FL, 2005), pp. 8.58–8.84.

P. N. Prasad, Introduction to biophotonics (Wiley Intersciences, 2003).

J. Homola, Surface Plasmon Resonance Based Sensors (2006), p. 251.

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Figures (5)

Fig. 1
Fig. 1

Optical set-up for polarimetric measurement.

Fig. 2
Fig. 2

(a) Theoretical angular polarimetric curve (APC) with different initial retardance α. (b) Comparison of APC (α=-Δϕ max solid lines) with SPR (dashed lines). Black curves are for tests in water and red curves after 10−2 RIU bulk changes; blue curves show influence of surface thin film deposition (10 nm, n=1.45).

Fig. 3
Fig. 3

(a) Phase sensitivity experiment (phase: black line, polarimetric signal: red line) (b) Experimental results for first harmonic with the same retardance as in Fig. 2(a).

Fig. 4
Fig. 4

(a) Angular Polarimetric Curve with different beam propagation geometry. (b) Working distance minimizing the influence of the convergence/divergence near the focal point; Inset: Measured propagation Gaussian beam near the focal point.

Fig. 5
Fig. 5

(a) Angular variation of the peak position for different refractive index solutions. (b) Responses of the system with different concentrations of Bovine Serum Albumin.

Equations (3)

Equations on this page are rendered with MathJax. Learn more.

F 1 = 2 J 1 ( M ) R p R s sin ( α + ( ϕ p ϕ s ) )
F 2 = 2 J 2 ( M ) R p R s cos ( α + ( ϕ p ϕ s ) )
ϕ + α = tan 1 ( F 1 J 2 ( M ) F 2 J 1 ( M ) )   and   I = R p R s = ( F 1 J 1 ( M ) ) 2 + ( F 2 J 2 ( M ) ) 2

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