Abstract

An investigation is reported of the identification and measurement of region of interest (ROI) in quantitative phase-contrast maps of biological cells by digital holographic microscopy. In particular, two different methods have been developed for in vitro bull sperm head morphometry analysis. We show that semen analysis can be accomplished by means of the proposed techniques . Extraction and measurement of various parameters are performed. It is demonstrated that both proposed methods are efficient to skim the data set in a preselective analysis for discarding anomalous data.

© 2011 OSA

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    [CrossRef] [PubMed]
  40. G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
    [CrossRef]

2011 (1)

2010 (4)

L. Camacho, V. Micó, Z. Zalevsky, and J. García, “Quantitative phase microscopy using defocusing by means of a spatial light modulator,” Opt. Express 18(7), 6755–6766 (2010).
[CrossRef] [PubMed]

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, “Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction,” Opt. Lett. 35(20), 3390–3392 (2010).
[CrossRef] [PubMed]

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

R. Puglisi, L. Krvavac, C. Bonacina, and A. Galli, “In vitro competitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility,” Zygote 18(04), 281–291 (2010).
[CrossRef] [PubMed]

2009 (1)

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Determination of 3D-region of interest using digital in-line holography with astigmatic Gaussian beams,” J. European Opt. Soc. Rapid Publications 4, 09038 (2009).
[CrossRef]

2008 (1)

2007 (1)

2006 (2)

L. Zhang and K. Wang, “New region of interest image coding and its applications for remote sensing image,” Chin. Opt. Lett. 4, 76–79 (2006).

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

2005 (2)

2004 (2)

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
[CrossRef]

P. Ferraro, S. De Nicola, G. Coppola, A. Finizio, D. Alfieri, and G. Pierattini, “Controlling image size as a function of distance and wavelength in Fresnel-transform reconstruction of digital holograms,” Opt. Lett. 29(8), 854–856 (2004).
[CrossRef] [PubMed]

2002 (2)

U. Schnars and W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13(9), R85–R101 (2002).
[CrossRef]

D. Comaniciu and P. Meer, “Mean-shift: a robust approach towards feature space analysis,” IEEE Trans. Pattern Anal. Mach. Intell. 24(5), 603–619 (2002).
[CrossRef]

1998 (1)

J. Weickert, B. H. Romeny, and M. A. Viergever, “Efficient and reliable schemes for nonlinear diffusion filtering,” IEEE Trans. Image Process. 7(3), 398–410 (1998).
[CrossRef] [PubMed]

1995 (1)

I. C. Macleod and D. S. Irvine, “The predictive value of computer-assisted semen analysis in the context of a donor insemination programme,” Hum. Reprod. 10(3), 580–586 (1995).
[PubMed]

1993 (1)

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

1992 (1)

J. M. DeJarnette, R. G. Saacke, J. Bame, and C. J. Vogler, “Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle,” J. Anim. Sci. 70(2), 484–491 (1992).
[PubMed]

1990 (2)

D. J. Jasko, D. H. Lein, and R. H. Foote, “Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988),” J. Am. Vet. Med. Assoc. 197(3), 389–394 (1990).
[PubMed]

P. Perona and J. Malik, “Scale-space and edge detection using anisotropic diffusion,” IEEE Trans. Pattern Anal. Mach. Intell. 12(7), 629–639 (1990).
[CrossRef]

1989 (1)

A. Galli, L. Paloschi, and F. Pizzi, “Some variability factors in the cytomorphological analysis of frozen bull semen,” Andrologia 21(2), 120–126 (1989).
[CrossRef] [PubMed]

1987 (2)

V. O. Sekoni and B. K. Gustafsson, “Seasonal variations in the incidence of sperm morphological abnormalities in dairy bulls regularly used for artificial insemination,” Br. Vet. J. 143(4), 312–317 (1987).
[PubMed]

M. C. Kot and M. A. Handel, “Binding of morphologically abnormal sperm to mouse egg zonae pellucidae in vitro,” Gamete Res. 18(1), 57–66 (1987).
[CrossRef] [PubMed]

1986 (2)

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

J. Canny, “A computational approach to edge detection,” IEEE Trans. Pattern Anal. Mach. Intell. PAMI-8(6), 679–698 (1986).
[CrossRef]

1979 (1)

N. Otsu, “A threshold selection method from gray-level histograms,” IEEE Trans. Syst. Man Cybern. 9(1), 62–66 (1979).
[CrossRef]

1955 (2)

G. Nomarski, “Differential microinterferometer with polarized waves,” J. Phys. Radium 16, 9S–13S (1955).

F. Zernike, “How I discovered phase contrast,” Science 121(3141), 345–349 (1955).
[CrossRef] [PubMed]

Acosta, A. A.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Alfieri, D.

Anastasio, M. A.

Balduzzi, D.

Bame, J.

J. M. DeJarnette, R. G. Saacke, J. Bame, and C. J. Vogler, “Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle,” J. Anim. Sci. 70(2), 484–491 (1992).
[PubMed]

Bloom, T. D.

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

Bonacina, C.

R. Puglisi, L. Krvavac, C. Bonacina, and A. Galli, “In vitro competitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility,” Zygote 18(04), 281–291 (2010).
[CrossRef] [PubMed]

Brunel, M.

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Determination of 3D-region of interest using digital in-line holography with astigmatic Gaussian beams,” J. European Opt. Soc. Rapid Publications 4, 09038 (2009).
[CrossRef]

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Digital in-line holography in thick optical systems: application to visualization in pipes,” Appl. Opt. 47(22), 4147–4157 (2008).
[CrossRef] [PubMed]

Camacho, L.

Canny, J.

J. Canny, “A computational approach to edge detection,” IEEE Trans. Pattern Anal. Mach. Intell. PAMI-8(6), 679–698 (1986).
[CrossRef]

Carapezza, E.

Coëtmellec, S.

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Determination of 3D-region of interest using digital in-line holography with astigmatic Gaussian beams,” J. European Opt. Soc. Rapid Publications 4, 09038 (2009).
[CrossRef]

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Digital in-line holography in thick optical systems: application to visualization in pipes,” Appl. Opt. 47(22), 4147–4157 (2008).
[CrossRef] [PubMed]

Colomb, T.

Comaniciu, D.

D. Comaniciu and P. Meer, “Mean-shift: a robust approach towards feature space analysis,” IEEE Trans. Pattern Anal. Mach. Intell. 24(5), 603–619 (2002).
[CrossRef]

Coppola, G.

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, “Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction,” Opt. Lett. 35(20), 3390–3392 (2010).
[CrossRef] [PubMed]

P. Ferraro, S. De Nicola, G. Coppola, A. Finizio, D. Alfieri, and G. Pierattini, “Controlling image size as a function of distance and wavelength in Fresnel-transform reconstruction of digital holograms,” Opt. Lett. 29(8), 854–856 (2004).
[CrossRef] [PubMed]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
[CrossRef]

Cuche, E.

De Nicola, S.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
[CrossRef]

P. Ferraro, S. De Nicola, G. Coppola, A. Finizio, D. Alfieri, and G. Pierattini, “Controlling image size as a function of distance and wavelength in Fresnel-transform reconstruction of digital holograms,” Opt. Lett. 29(8), 854–856 (2004).
[CrossRef] [PubMed]

Debeir, O.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

Decaestecker, Ch.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

DeJarnette, J. M.

J. M. DeJarnette, R. G. Saacke, J. Bame, and C. J. Vogler, “Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle,” J. Anim. Sci. 70(2), 484–491 (1992).
[PubMed]

Depeursinge, C.

Di Caprio, G.

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, “Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction,” Opt. Lett. 35(20), 3390–3392 (2010).
[CrossRef] [PubMed]

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

Dubois, F.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

DuToit, T. C.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Emery, Y.

Ferraro, P.

Finizio, A.

Foote, R. H.

D. J. Jasko, D. H. Lein, and R. H. Foote, “Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988),” J. Am. Vet. Med. Assoc. 197(3), 389–394 (1990).
[PubMed]

Franken, D. R.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Galli, A.

L. Miccio, A. Finizio, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, “Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization,” Biomed. Opt. Express 2(2), 331–344 (2011).
[CrossRef] [PubMed]

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, “Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction,” Opt. Lett. 35(20), 3390–3392 (2010).
[CrossRef] [PubMed]

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

R. Puglisi, L. Krvavac, C. Bonacina, and A. Galli, “In vitro competitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility,” Zygote 18(04), 281–291 (2010).
[CrossRef] [PubMed]

A. Galli, L. Paloschi, and F. Pizzi, “Some variability factors in the cytomorphological analysis of frozen bull semen,” Andrologia 21(2), 120–126 (1989).
[CrossRef] [PubMed]

García, J.

Gioffré, M.

Gioffré, M. A.

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

Grilli, S.

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, “Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction,” Opt. Lett. 35(20), 3390–3392 (2010).
[CrossRef] [PubMed]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
[CrossRef]

Gustafsson, B. K.

V. O. Sekoni and B. K. Gustafsson, “Seasonal variations in the incidence of sperm morphological abnormalities in dairy bulls regularly used for artificial insemination,” Br. Vet. J. 143(4), 312–317 (1987).
[PubMed]

Handel, M. A.

M. C. Kot and M. A. Handel, “Binding of morphologically abnormal sperm to mouse egg zonae pellucidae in vitro,” Gamete Res. 18(1), 57–66 (1987).
[CrossRef] [PubMed]

Iodice, M.

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
[CrossRef]

Irvine, D. S.

I. C. Macleod and D. S. Irvine, “The predictive value of computer-assisted semen analysis in the context of a donor insemination programme,” Hum. Reprod. 10(3), 580–586 (1995).
[PubMed]

Jasko, D. J.

D. J. Jasko, D. H. Lein, and R. H. Foote, “Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988),” J. Am. Vet. Med. Assoc. 197(3), 389–394 (1990).
[PubMed]

Javidi, B.

Jüptner, W. P. O.

U. Schnars and W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13(9), R85–R101 (2002).
[CrossRef]

Katz, D. F.

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

Kiss, R.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

Kot, M. C.

M. C. Kot and M. A. Handel, “Binding of morphologically abnormal sperm to mouse egg zonae pellucidae in vitro,” Gamete Res. 18(1), 57–66 (1987).
[CrossRef] [PubMed]

Kruger, T. F.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Krvavac, L.

R. Puglisi, L. Krvavac, C. Bonacina, and A. Galli, “In vitro competitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility,” Zygote 18(04), 281–291 (2010).
[CrossRef] [PubMed]

Lebrun, D.

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Determination of 3D-region of interest using digital in-line holography with astigmatic Gaussian beams,” J. European Opt. Soc. Rapid Publications 4, 09038 (2009).
[CrossRef]

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Digital in-line holography in thick optical systems: application to visualization in pipes,” Appl. Opt. 47(22), 4147–4157 (2008).
[CrossRef] [PubMed]

Legros, J.-C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

Lein, D. H.

D. J. Jasko, D. H. Lein, and R. H. Foote, “Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988),” J. Am. Vet. Med. Assoc. 197(3), 389–394 (1990).
[PubMed]

Lewis, E. L.

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

Lombard, C. J.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Macleod, I. C.

I. C. Macleod and D. S. Irvine, “The predictive value of computer-assisted semen analysis in the context of a donor insemination programme,” Hum. Reprod. 10(3), 580–586 (1995).
[PubMed]

Magistretti, P. J.

Malik, J.

P. Perona and J. Malik, “Scale-space and edge detection using anisotropic diffusion,” IEEE Trans. Pattern Anal. Mach. Intell. 12(7), 629–639 (1990).
[CrossRef]

Marquet, P.

Meer, P.

D. Comaniciu and P. Meer, “Mean-shift: a robust approach towards feature space analysis,” IEEE Trans. Pattern Anal. Mach. Intell. 24(5), 603–619 (2002).
[CrossRef]

Menkveld, R.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Miccio, L.

Micó, V.

Monnom, O.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

Moon, I.

Niswander, P. W.

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

Nomarski, G.

G. Nomarski, “Differential microinterferometer with polarized waves,” J. Phys. Radium 16, 9S–13S (1955).

Oehninger, S. C.

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Otsu, N.

N. Otsu, “A threshold selection method from gray-level histograms,” IEEE Trans. Syst. Man Cybern. 9(1), 62–66 (1979).
[CrossRef]

Overstreet, J. W.

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

Paloschi, L.

A. Galli, L. Paloschi, and F. Pizzi, “Some variability factors in the cytomorphological analysis of frozen bull semen,” Andrologia 21(2), 120–126 (1989).
[CrossRef] [PubMed]

Pan, X.

Paturzo, M.

Perona, P.

P. Perona and J. Malik, “Scale-space and edge detection using anisotropic diffusion,” IEEE Trans. Pattern Anal. Mach. Intell. 12(7), 629–639 (1990).
[CrossRef]

Pierattini, G.

Pizzi, F.

A. Galli, L. Paloschi, and F. Pizzi, “Some variability factors in the cytomorphological analysis of frozen bull semen,” Andrologia 21(2), 120–126 (1989).
[CrossRef] [PubMed]

Puglisi, R.

L. Miccio, A. Finizio, R. Puglisi, D. Balduzzi, A. Galli, and P. Ferraro, “Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization,” Biomed. Opt. Express 2(2), 331–344 (2011).
[CrossRef] [PubMed]

G. Coppola, G. Di Caprio, M. Gioffré, R. Puglisi, D. Balduzzi, A. Galli, L. Miccio, M. Paturzo, S. Grilli, A. Finizio, and P. Ferraro, “Digital self-referencing quantitative phase microscopy by wavefront folding in holographic image reconstruction,” Opt. Lett. 35(20), 3390–3392 (2010).
[CrossRef] [PubMed]

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

R. Puglisi, L. Krvavac, C. Bonacina, and A. Galli, “In vitro competitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility,” Zygote 18(04), 281–291 (2010).
[CrossRef] [PubMed]

Rappaz, B.

Romeny, B. H.

J. Weickert, B. H. Romeny, and M. A. Viergever, “Efficient and reliable schemes for nonlinear diffusion filtering,” IEEE Trans. Image Process. 7(3), 398–410 (1998).
[CrossRef] [PubMed]

Saacke, R. G.

J. M. DeJarnette, R. G. Saacke, J. Bame, and C. J. Vogler, “Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle,” J. Anim. Sci. 70(2), 484–491 (1992).
[PubMed]

Saffioti, N.

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

Samuels, S. J.

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

Schnars, U.

U. Schnars and W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13(9), R85–R101 (2002).
[CrossRef]

Sekoni, V. O.

V. O. Sekoni and B. K. Gustafsson, “Seasonal variations in the incidence of sperm morphological abnormalities in dairy bulls regularly used for artificial insemination,” Br. Vet. J. 143(4), 312–317 (1987).
[PubMed]

Van Ham, P.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

Verrier, N.

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Determination of 3D-region of interest using digital in-line holography with astigmatic Gaussian beams,” J. European Opt. Soc. Rapid Publications 4, 09038 (2009).
[CrossRef]

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Digital in-line holography in thick optical systems: application to visualization in pipes,” Appl. Opt. 47(22), 4147–4157 (2008).
[CrossRef] [PubMed]

Viergever, M. A.

J. Weickert, B. H. Romeny, and M. A. Viergever, “Efficient and reliable schemes for nonlinear diffusion filtering,” IEEE Trans. Image Process. 7(3), 398–410 (1998).
[CrossRef] [PubMed]

Vogler, C. J.

J. M. DeJarnette, R. G. Saacke, J. Bame, and C. J. Vogler, “Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle,” J. Anim. Sci. 70(2), 484–491 (1992).
[PubMed]

Wang, K.

Weickert, J.

J. Weickert, B. H. Romeny, and M. A. Viergever, “Efficient and reliable schemes for nonlinear diffusion filtering,” IEEE Trans. Image Process. 7(3), 398–410 (1998).
[CrossRef] [PubMed]

Yeom, S.

Yourassowsky, C.

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

Zalevsky, Z.

Zernike, F.

F. Zernike, “How I discovered phase contrast,” Science 121(3141), 345–349 (1955).
[CrossRef] [PubMed]

Zhang, L.

Andrologia (1)

A. Galli, L. Paloschi, and F. Pizzi, “Some variability factors in the cytomorphological analysis of frozen bull semen,” Andrologia 21(2), 120–126 (1989).
[CrossRef] [PubMed]

Appl. Opt. (1)

Biomed. Opt. Express (1)

Br. Vet. J. (1)

V. O. Sekoni and B. K. Gustafsson, “Seasonal variations in the incidence of sperm morphological abnormalities in dairy bulls regularly used for artificial insemination,” Br. Vet. J. 143(4), 312–317 (1987).
[PubMed]

Chin. Opt. Lett. (1)

Fertil. Steril. (1)

T. F. Kruger, T. C. DuToit, D. R. Franken, A. A. Acosta, S. C. Oehninger, R. Menkveld, and C. J. Lombard, “A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading,” Fertil. Steril. 59(1), 202–209 (1993).
[PubMed]

Gamete Res. (1)

M. C. Kot and M. A. Handel, “Binding of morphologically abnormal sperm to mouse egg zonae pellucidae in vitro,” Gamete Res. 18(1), 57–66 (1987).
[CrossRef] [PubMed]

Hum. Reprod. (1)

I. C. Macleod and D. S. Irvine, “The predictive value of computer-assisted semen analysis in the context of a donor insemination programme,” Hum. Reprod. 10(3), 580–586 (1995).
[PubMed]

IEEE J. Sel. Top. Quantum Electron. (1)

G. Di Caprio, M. A. Gioffré, N. Saffioti, S. Grilli, P. Ferraro, R. Puglisi, D. Balduzzi, A. Galli, and G. Coppola, “Quantitative label-free animal sperm imaging by means of digital holographic microscopy,” IEEE J. Sel. Top. Quantum Electron. 16(4), 833–840 (2010).
[CrossRef]

IEEE Trans. Image Process. (1)

J. Weickert, B. H. Romeny, and M. A. Viergever, “Efficient and reliable schemes for nonlinear diffusion filtering,” IEEE Trans. Image Process. 7(3), 398–410 (1998).
[CrossRef] [PubMed]

IEEE Trans. Pattern Anal. Mach. Intell. (3)

J. Canny, “A computational approach to edge detection,” IEEE Trans. Pattern Anal. Mach. Intell. PAMI-8(6), 679–698 (1986).
[CrossRef]

P. Perona and J. Malik, “Scale-space and edge detection using anisotropic diffusion,” IEEE Trans. Pattern Anal. Mach. Intell. 12(7), 629–639 (1990).
[CrossRef]

D. Comaniciu and P. Meer, “Mean-shift: a robust approach towards feature space analysis,” IEEE Trans. Pattern Anal. Mach. Intell. 24(5), 603–619 (2002).
[CrossRef]

IEEE Trans. Syst. Man Cybern. (1)

N. Otsu, “A threshold selection method from gray-level histograms,” IEEE Trans. Syst. Man Cybern. 9(1), 62–66 (1979).
[CrossRef]

J. Am. Vet. Med. Assoc. (1)

D. J. Jasko, D. H. Lein, and R. H. Foote, “Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988),” J. Am. Vet. Med. Assoc. 197(3), 389–394 (1990).
[PubMed]

J. Androl. (1)

D. F. Katz, J. W. Overstreet, S. J. Samuels, P. W. Niswander, T. D. Bloom, and E. L. Lewis, “Morphometric analysis of spermatozoa in the assessment of human male fertility,” J. Androl. 7(4), 203–210 (1986).
[PubMed]

J. Anim. Sci. (1)

J. M. DeJarnette, R. G. Saacke, J. Bame, and C. J. Vogler, “Accessory sperm: their importance to fertility and embryo quality, and attempts to alter their numbers in artificially inseminated cattle,” J. Anim. Sci. 70(2), 484–491 (1992).
[PubMed]

J. Biomed. Opt. (1)

F. Dubois, C. Yourassowsky, O. Monnom, J.-C. Legros, O. Debeir, P. Van Ham, R. Kiss, and Ch. Decaestecker, “Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration,” J. Biomed. Opt. 11(5), 054032 (2006).
[CrossRef] [PubMed]

J. European Opt. Soc. Rapid Publications (1)

N. Verrier, S. Coëtmellec, M. Brunel, and D. Lebrun, “Determination of 3D-region of interest using digital in-line holography with astigmatic Gaussian beams,” J. European Opt. Soc. Rapid Publications 4, 09038 (2009).
[CrossRef]

J. Phys. Radium (1)

G. Nomarski, “Differential microinterferometer with polarized waves,” J. Phys. Radium 16, 9S–13S (1955).

Meas. Sci. Technol. (2)

U. Schnars and W. P. O. Jüptner, “Digital recording and numerical reconstruction of holograms,” Meas. Sci. Technol. 13(9), R85–R101 (2002).
[CrossRef]

G. Coppola, P. Ferraro, M. Iodice, S. De Nicola, A. Finizio, and S. Grilli, “A digital holographic microscope for complete characterization of microelectromechanical systems,” Meas. Sci. Technol. 15(3), 529–539 (2004).
[CrossRef]

Opt. Express (2)

Opt. Lett. (4)

Science (1)

F. Zernike, “How I discovered phase contrast,” Science 121(3141), 345–349 (1955).
[CrossRef] [PubMed]

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R. Puglisi, L. Krvavac, C. Bonacina, and A. Galli, “In vitro competitive binding index using fluorochrome-labelled spermatozoa for predicting bull fertility,” Zygote 18(04), 281–291 (2010).
[CrossRef] [PubMed]

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H. Carrillo, J. Villarreal, M. Sotaquirà, A. Goelkel, and R. Gutierrez, “A computer aided tool for the assessment of human sperm morphology,” in Proceedings of the 7th IEEE International Conference on Bioinformatics and Bioengineering, 2007. BIBE 2007 (2007), pp. 1152–1157.

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Supplementary Material (1)

» Media 1: AVI (271 KB)     

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Figures (10)

Fig. 1
Fig. 1

phase reconstructions of sperm. The maximum value is (a) before the beginning of head and (b) inside the head.

Fig. 2
Fig. 2

(a) masks after rotational searching on Fig. 1(a); the red box is the mask centered in the maximum value, the blue box begins on the maximum value. Obviously, the extraction algorithm chooses the blue box. (b) first frame of video Media 1 that shows the extraction algorithm for the blue box.

Fig. 3
Fig. 3

Schematic view of the experimental setup.

Fig. 4
Fig. 4

(a 1-4) original, (b 1-4) extraction of Algorithm 1, (c 1-4) extraction of Algorithm 2, (d 1-4) extraction by Canny edge detection.

Fig. 5
Fig. 5

detection of sperm head for phase map in Fig. 1 using Algorithm 1: (a) and (d) are the results of the diffusion filtering, (b) and (e) are the results of thresholding, (c) and (f) are the best fit ellipses.

Fig. 6
Fig. 6

detection of sperm head for phase map in Fig. 1 using Algorithm 2: (a) and (d) are the results of the denoising, (b) and (e) are the results of extraction algorithm, (c) and (f) are the best fit ellipses.

Fig. 7
Fig. 7

Estimated PDF of area on data set with two proposed algorithms

Fig. 8
Fig. 8

Estimated PDFs of other parameters of best fit ellipse for both algorithms.

Fig. 9
Fig. 9

Recorded holograms of sperm head for distortion on the head. (b) to (d) are related to Algorithm 1; (e) to (g) are related to Algorithm 2.

Fig. 10
Fig. 10

Recorded holograms of sperm head for rotated head. (b) to (d) are related to Algorithm 1; (e) to (g) are related to Algorithm 2.

Equations (10)

Equations on this page are rendered with MathJax. Learn more.

t u=div( g | u σ | 2 u )    on  Ω×(0,)
u( x,0 )=f( x )onΩ
n u=0onΩ×( 0, )
u σ =( G σ u )
G σ = 1 2π σ 2 exp( | x | 2 2 σ 2 )
g( s 2 )={ 1 s 2 =0 1exp( c ( s/λ ) 8 ) s 2 >0
λ=1.4826median{ | f |median{ | f | } }
p ^ N ( y )= 1 N i=1 N 1 h N K( y y i h N )
K N ( x )=exp( 1 2 x )x1
ShapeFactor= ( 1-E ) P 2 4πA

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