Abstract

Layered peptide array (LPA) system enables multiplex screening of biomarkers [1-3]. One of the main problems of the LPA system is the screening of the layered-membranes stack. Nowadays, each membrane is imaged separately using conventional fluorescent imaging. This process is time consuming and requires extensive manual interaction. This paper describes a general solution for optical imaging of a layered grid medium using photogrammetric methods. The suggested method enables visualization of the LPA membranes stack by using only two images of the stack. This study is a proof of concept of the suggested solution using MATLAB simulation and phantom experiments.

© 2011 OSA

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  1. G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
    [CrossRef] [PubMed]
  2. G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
    [CrossRef] [PubMed]
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2007

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

2006

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

2005

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

2002

H. Mitchell and I. Newton, “Medical photogrammetric measurement: overview and prospects,” ISPRS J. Photogramm. Remote Sens. 56(5-6), 286–294 (2002).
[CrossRef]

2001

P. Rosin, “Unimodal thresholding,” Pattern Recognit. 34(11), 2083–2096 (2001).
[CrossRef]

R. Westaway, S. Lane, and D. Hicks, “Remote sensing of clear-water, shallow, gravel-bed rivers using digital photogrammetry,” Photogramm. Eng. Remote Sensing 67, 1271–1282 (2001).

2000

A. Fusiello, E. Trucco, and A. Verri, “A compact algorithm for rectification of stereo pairs,” Mach. Vis. Appl. 12(1), 16–22 (2000).
[CrossRef]

1993

H. Maas, A. Gruen, and D. Papantoniou, “Particle tracking velocimetry in three-dimensional flows,” Exp. Fluids 15(2), 133–146 (1993).
[CrossRef]

L. Vincent, “Morphological grayscale reconstruction in image analysis: applications and efficient algorithms,” IEEE Trans. Image Process. 2(2), 176–201 (1993).
[CrossRef] [PubMed]

1990

F. Meyer and S. Beucher, “Morphological segmentation,” J. Vis. Commun. Image Represent. 1(1), 21–46 (1990).
[CrossRef]

1977

G. W. Zack, W. E. Rogers, and S. A. Latt, “Automatic measurement of sister chromatid exchange frequency,” J. Histochem. Cytochem. 25(7), 741–753 (1977).
[CrossRef] [PubMed]

Beucher, S.

F. Meyer and S. Beucher, “Morphological segmentation,” J. Vis. Commun. Image Represent. 1(1), 21–46 (1990).
[CrossRef]

Chuaqui, R. F.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

De Grand, A. M.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Emmert-Buck, M. R.

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Erickson, H. S.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Flaig, M. J.

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

Frangioni, J. V.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Fusiello, A.

A. Fusiello, E. Trucco, and A. Verri, “A compact algorithm for rectification of stereo pairs,” Mach. Vis. Appl. 12(1), 16–22 (2000).
[CrossRef]

Gannot, G.

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Gillespie, J. W.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Gogbashian, A.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Gruen, A.

H. Maas, A. Gruen, and D. Papantoniou, “Particle tracking velocimetry in three-dimensional flows,” Exp. Fluids 15(2), 133–146 (1993).
[CrossRef]

Hartmann, D. P.

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Hewitt, S. M.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

Hicks, D.

R. Westaway, S. Lane, and D. Hicks, “Remote sensing of clear-water, shallow, gravel-bed rivers using digital photogrammetry,” Photogramm. Eng. Remote Sensing 67, 1271–1282 (2001).

Knezevic, V.

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Lane, S.

R. Westaway, S. Lane, and D. Hicks, “Remote sensing of clear-water, shallow, gravel-bed rivers using digital photogrammetry,” Photogramm. Eng. Remote Sensing 67, 1271–1282 (2001).

Latt, S. A.

G. W. Zack, W. E. Rogers, and S. A. Latt, “Automatic measurement of sister chromatid exchange frequency,” J. Histochem. Cytochem. 25(7), 741–753 (1977).
[CrossRef] [PubMed]

Laurence, R. G.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Leakan, R. A.

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Lee, D. S.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Lomnes, S. J.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Maas, H.

H. Maas, A. Gruen, and D. Papantoniou, “Particle tracking velocimetry in three-dimensional flows,” Exp. Fluids 15(2), 133–146 (1993).
[CrossRef]

Marcus, E. M.

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

Meyer, F.

F. Meyer and S. Beucher, “Morphological segmentation,” J. Vis. Commun. Image Represent. 1(1), 21–46 (1990).
[CrossRef]

Mitchell, H.

H. Mitchell and I. Newton, “Medical photogrammetric measurement: overview and prospects,” ISPRS J. Photogramm. Remote Sens. 56(5-6), 286–294 (2002).
[CrossRef]

Morgan, T. G.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Newton, I.

H. Mitchell and I. Newton, “Medical photogrammetric measurement: overview and prospects,” ISPRS J. Photogramm. Remote Sens. 56(5-6), 286–294 (2002).
[CrossRef]

Ohnishi, S.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Papantoniou, D.

H. Maas, A. Gruen, and D. Papantoniou, “Particle tracking velocimetry in three-dimensional flows,” Exp. Fluids 15(2), 133–146 (1993).
[CrossRef]

Pietrzykowski, M.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

Pinto, P. A.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

Richardson, A. M.

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

Rogers, W. E.

G. W. Zack, W. E. Rogers, and S. A. Latt, “Automatic measurement of sister chromatid exchange frequency,” J. Histochem. Cytochem. 25(7), 741–753 (1977).
[CrossRef] [PubMed]

Rosin, P.

P. Rosin, “Unimodal thresholding,” Pattern Recognit. 34(11), 2083–2096 (2001).
[CrossRef]

Tangrea, M. A.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Trucco, E.

A. Fusiello, E. Trucco, and A. Verri, “A compact algorithm for rectification of stereo pairs,” Mach. Vis. Appl. 12(1), 16–22 (2000).
[CrossRef]

Verri, A.

A. Fusiello, E. Trucco, and A. Verri, “A compact algorithm for rectification of stereo pairs,” Mach. Vis. Appl. 12(1), 16–22 (2000).
[CrossRef]

Vincent, L.

L. Vincent, “Morphological grayscale reconstruction in image analysis: applications and efficient algorithms,” IEEE Trans. Image Process. 2(2), 176–201 (1993).
[CrossRef] [PubMed]

Wallis, B. S.

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

Westaway, R.

R. Westaway, S. Lane, and D. Hicks, “Remote sensing of clear-water, shallow, gravel-bed rivers using digital photogrammetry,” Photogramm. Eng. Remote Sensing 67, 1271–1282 (2001).

Zack, G. W.

G. W. Zack, W. E. Rogers, and S. A. Latt, “Automatic measurement of sister chromatid exchange frequency,” J. Histochem. Cytochem. 25(7), 741–753 (1977).
[CrossRef] [PubMed]

Clin. Chim. Acta

G. Gannot, M. A. Tangrea, A. M. Richardson, M. J. Flaig, S. M. Hewitt, E. M. Marcus, M. R. Emmert-Buck, and R. F. Chuaqui, “Layered expression scanning: multiplex molecular analysis of diverse life science platforms,” Clin. Chim. Acta 376(1-2), 9–16 (2007).
[CrossRef] [PubMed]

Exp. Fluids

H. Maas, A. Gruen, and D. Papantoniou, “Particle tracking velocimetry in three-dimensional flows,” Exp. Fluids 15(2), 133–146 (1993).
[CrossRef]

IEEE Trans. Image Process.

L. Vincent, “Morphological grayscale reconstruction in image analysis: applications and efficient algorithms,” IEEE Trans. Image Process. 2(2), 176–201 (1993).
[CrossRef] [PubMed]

ISPRS J. Photogramm. Remote Sens.

H. Mitchell and I. Newton, “Medical photogrammetric measurement: overview and prospects,” ISPRS J. Photogramm. Remote Sens. 56(5-6), 286–294 (2002).
[CrossRef]

J. Biomed. Opt.

A. M. De Grand, S. J. Lomnes, D. S. Lee, M. Pietrzykowski, S. Ohnishi, T. G. Morgan, A. Gogbashian, R. G. Laurence, and J. V. Frangioni, “Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons,” J. Biomed. Opt. 11(1), 014007 (2006).
[CrossRef] [PubMed]

J. Histochem. Cytochem.

G. W. Zack, W. E. Rogers, and S. A. Latt, “Automatic measurement of sister chromatid exchange frequency,” J. Histochem. Cytochem. 25(7), 741–753 (1977).
[CrossRef] [PubMed]

J. Mol. Diagn.

G. Gannot, M. A. Tangrea, H. S. Erickson, P. A. Pinto, S. M. Hewitt, R. F. Chuaqui, J. W. Gillespie, and M. R. Emmert-Buck, “Layered peptide array for multiplex immunohistochemistry,” J. Mol. Diagn. 9(3), 297–304 (2007).
[CrossRef] [PubMed]

G. Gannot, M. A. Tangrea, J. W. Gillespie, H. S. Erickson, B. S. Wallis, R. A. Leakan, V. Knezevic, D. P. Hartmann, R. F. Chuaqui, and M. R. Emmert-Buck, “Layered peptide arrays: high-throughput antibody screening of clinical samples,” J. Mol. Diagn. 7(4), 427–436 (2005).
[CrossRef] [PubMed]

J. Vis. Commun. Image Represent.

F. Meyer and S. Beucher, “Morphological segmentation,” J. Vis. Commun. Image Represent. 1(1), 21–46 (1990).
[CrossRef]

Mach. Vis. Appl.

A. Fusiello, E. Trucco, and A. Verri, “A compact algorithm for rectification of stereo pairs,” Mach. Vis. Appl. 12(1), 16–22 (2000).
[CrossRef]

Pattern Recognit.

P. Rosin, “Unimodal thresholding,” Pattern Recognit. 34(11), 2083–2096 (2001).
[CrossRef]

Photogramm. Eng. Remote Sensing

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Figures (9)

Fig. 1
Fig. 1

Schematic of the LPA System [1].

Fig. 2
Fig. 2

An overview of the algorithm.

Fig. 3
Fig. 3

Schematic drawing of the cameras adjustment.

Fig. 4
Fig. 4

Calibration images with 1mm grid.

Fig. 5
Fig. 5

The experiment set-up.

Fig. 6
Fig. 6

Agar gel phantom preparation tools.

Fig. 7
Fig. 7

Results for sample with 3 layers and 20 points of an Agar gel phantom. The top row is the captured images. The middle rows are the images after noise reduction with the segmented line marked in white. The bottom row is the Layer separation of the fluorescent points. The filled circles are the detected dots with their real dimension and the positions of the reconstructed 3D points are marked with a plus sign.

Fig. 8
Fig. 8

Results for sample with 3 layers and 27 points of a Perspex phantom. The top row is the captured images. The middle rows are the images after noise reduction with the segmented line marked in white. The bottom row is the Layer separation of the fluorescent points. The filled circles are the detected dots with their real dimension and the positions of the reconstructed 3D points are marked with a plus sign.

Fig. 9
Fig. 9

3D reconstruction Results for sample with 3 layers and 20 points of an Agar gel phantom.

Tables (3)

Tables Icon

Table1 Agar Gel Phantoms Results Evaluation

Tables Icon

Table 2 Perspex Phantom Results Evaluation

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Table 3 Simulation with the Experimental Orientation- Results Evaluation

Metrics